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Isolation and structural characterization of a polysaccharide FCAP1 from the fruit of Cornus officinalis

CARBOHYDRATE research 2010年第13期345卷 1909-1913页

作者： Yang, Liyan Wang, Zhongfu Huang, Linjuan NW Univ Xian Life Sci Coll Prov Key Lab Biotechnol Key LabMinist Educ Western China Resource Biol & Xian 710069 Peoples R China

A water-soluble polysaccharide, FCAP1, was isolated from an alkaline extract from the fruits of Cornus officinalis. Its molecular weight was 34.5 kDa. Monosaccharide composition analysis revealed that it was composed of fucose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 0.29:0.19:1.74:1:3.30:1.10. On the basis of partial acid hydrolysis and methylation analysis, FCAP1 was shown to be a highly branched polysaccharide with a backbone of beta-(1 -> 4)-linked-glucose partially substituted at the O-6 position with xylopyranose residues. The branches were composed of (1 -> 3)-linked-Ara, (1 -> 4)-linked-Man, (1 -> 4,6)-linked-Man, (1 -> 4)-linked-Glc, and (1 -> 2)-linked-Gal. Arabinose. fucose, and galactose were located at the terminal of the branches. The structure was further elucidated by a specific enzymatic degradation with an endo-beta-(1 -> 4)-glucanase and MALDI-TOF-MS analysis. Oligosaccharides generated from FCAP1 indicated that FCAP1 contained)0((G-type and XXG-type xyloglucan fragments. (C) 2010 Elsevier Ltd. All rights reserved.

关键词： Cornus officinalis Polysaccharide Structure Xyloglucan

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LEAF AREA INDEX RETRIEVAL FROM REMotELY SENSED DATA: SCALING EFFECT AND PROPAGATION MECHANISMS 30

LEAF AREA INDEX RETRIEVAL FROM REMOTELY SENSED DATA: SCALING...

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30th IEEE International Geoscience and Remote Sensing Symposium (IGARSS) on Remote Sensing - Global Vision for Local Action

作者： Wu, Hua Tang, Bo-Hui Li, Chuanrong Li, Zhao-Liang Chinese Acad Sci State Key Lab Resources & Environm Informat Syst Inst Geog Sci & Nat Resources Res Beijing 100101 Peoples R China Chinese Acad Sci Acad Optoelectron Beijing Peoples R China CNRS LSIIT UdS F-67412 Illkirch Graffenstaden France

ISBN: (纸本)9781424495665

This paper makes an attempt to address the scaling problem of Leaf Area Index (LAI) and to analyze the propagation of scaling effect of LAI. On the basis of the Taylor series expansion and following the general scaling procedure, it is demonstrated that the magnitude of the scaling effect is the product of the degree of the non-linearity of the retrieval model and the spatial heterogeneity of input variables involved in this model. Finally, a scaling correction model is proposed to correct for the scaling effect of LAI. The validation using the simulated data indicates that the proposed scaling correction model of LAI gives promising accuracy if the spatial heterogeneity is well characterized by its wavelet variance. The RMSE and relative error of retrieved LAI induced by the scale effect can be greatly reduced after scaling correction. The scaling propagation analysis of LAI reveals that the scaling effects caused by several non-linear components may compensate for each other, which would enhance our confidence in using LAI product over heterogeneity areas.

关键词： Leaf area index Scaling Scaling effect Spatial heterogeneity Model non-linearity

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MELTING BEHAVIOR AND THE GRUNEISEN PARAMETER OF NaCl AT hiGH PRESSURE: A MOLECULAR DYNAMICAL STUDY

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INTERNATIONAL JOURNAL OF MODERN PHYSICS B 2010年第3期24卷 331-341页

作者： Cui, Shouxin Cai, Lingcang Hu, Haiquan Gong, Zizheng Zhao, Jijun Liaocheng Univ Sch Phys Sci & Informat Technol Liaocheng 252059 Peoples R China Inst Fluid Phys Lab Shock Wave & Detonat Phys Res Mianyang 621900 Peoples R China SW Jiaotong Univ Inst High Pressure & High Temp Phys Chengdu 610031 Peoples R China Dalian Univ Technol Coll Adv Sci & Technol State Key Lab Mat Modificat Laser Elect & Ion Bea Dalian 116024 Peoples R China

The improved Tosi-Fumi pair potential has been employed to simulate the melting behavior and Gruneisen parameters of sodium chloride (NaCl) using molecular dynamics (MD) method at constant volume. The melting curve of NaCl is compared with the experimental data and other calculations in a pressure range from 0 to 144 GPa. The simulated results validate the amount of 20% superheating of the NaCl solid and yield an approximate power law dependence of the Gruneisen parameter (gamma) on compression gamma = gamma(0)(V/V-0)(q), with q approximate to 1.078, in the temperature range from 298 to 1073 K and pressure range from 0 to 60 GPa.

关键词： NaCl melting Gruneisen parameter high pressure molecular dynamics

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The serum interleukin-18 is a potential marker for development of post-stroke depression

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NEUROLOGICAL research 2010年第4期32卷 340-346页

作者： Yang, Lingli Zhang, Zhijun Sun, Dingming Xu, Zhi Zhang, Xiangrong Li, Lingjiang Southeast Univ Dept Neurol Affiliated ZhongDa Hosp Nanjing 210009 Peoples R China Southeast Univ Neuropsychiat Res Inst Nanjing 210009 Peoples R China Southeast Univ Coll Med Nanjing 210009 Peoples R China Cent S Univ Xiangya Hosp 2 Mental Hlth Inst Changsha Hunan Peoples R China

Objective: Depression is a common mood disorder affecting stroke patients. It is associated with poorer outcome and increased mortality in stroke patients. The aim of this work was to test whether serum levels of proinflammatory cytokines are correlated with the development of depression after stroke. Methods: One hundred ischemic stroke patients admitted to the hospital within the first 24 hours after stroke onset were consecutively recruited and followed up for 6 months. The 17-item Hamilton Depression Rating Scale (HDRS) and Montgomery-Asberg Depression Rating Scale (MADRS) were used to screen for depressive symptoms on days 3, 7 and 14 after admission and at 6 months after stroke onset. Based on the symptoms elicited from these two scales, diagnoses of depression were made in accordance with DSM-IV criteria for post-stroke depression. Serum levels of proinflammatory cytokines (IL-6, IL-18 and TNF-alpha) of all the patients were determined by ELISA on both days 1 and 7 after admission. Meanwhile, 50 healthy control subjects were also recruited;they underwent measurement of serum levels of proinflammatory cytokines once. Results: Thirty-seven patients (37.0%) were diagnosed as having major depression at 2 weeks. Serum IL-18 on both days 1 and 7 was significantly higher in both post-stroke depression patients and non-post-stroke depression patients than in normal controls. Serum IL-18 on day 7 was significantly higher in post-stroke depression patients than in non-post-stroke depression patients. Serum IL-18>377.84 pg/ml on day 7 was independently associated with incident post-stroke depression at the acute stage of stroke (odds ratio: 12.280, 95% confidence interval: 3.848-39.190, p<0.001 after adjustment). At 6 months, 31 patients (33.0%) were diagnosed with major depression. Serum IL-18 >376.67 pg/ml on day 7 was independently associated with post-stroke depression at 6 months (odds ratio: 7.431, 95% confidence interval: 1.741-31.712, p=0.007 after adjustment).

关键词： Post-stroke depression biomarker interleukin-18

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A new car-following model yielding log-normal type headways distributions

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Chinese Physics B 2010年第2期19卷 180-185页

作者：李力王法姜锐胡坚明吉岩 Tsinghua National Laboratory for Information Science and Technology (TNList) Department of AutomationTsinghua University School of Engineering Science University of Science and Technology of China

Modeling time headways between vehicles has attracted increasing interest in the traffic flow research field recently, because the corresponding statistics help to reveal the intrinsic interactions governing the vehicle dynamics. However, most previous micro-simulation models cannot yield the observed log-normal distributed headways. This paper designs a new car-following model inspired by the Galton board to reproduce the observed time-headway distributions as well as the complex traffic phenomena. The consistency between the empirical data and the simulation results indicates that this new car-following model provides a reasonable description of the car-following behaviours.

关键词： traffic flow car-following log-normal distribution

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Microstructure and high tensile strength of Mg-10Gd-2Y-0.5Zr alloy by solid-state recycling

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MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING 2010年第2期528卷 715-720页

作者： Peng, Tao Wang, Qudong Han, York Zheng, Jiang Guo, Wei Shanghai Jiao Tong Univ Natl Engn Res Ctr Light Alloy Net Forming Shanghai 200240 Peoples R China Shanghai Jiao Tong Univ State Key Lab Met Matrix Composites Shanghai 200240 Peoples R China Monash Univ ARC Ctr Excellence Design Light Met Clayton Vic 3800 Australia

In general, the tensile strengths of the solid-recycled Mg alloys are increased by T6 treatment after extrusion. For Mg alloy with high rare earth (RE) content, it is difficult to obtain the desired higher tensile strength compared with those extruded ones from the original ingots. In this paper, according to some characteristic of Mg-RE alloys, effect of heat treatment and extrusion temperature on the properties of the recycled Mg-10Gd-2Y-0.5Zr was studied. The results show that the aging response in the recycled specimens is similar to that in as-extruded reference samples. The deformation temperature is increased in order to inhibit Mg-24(Gd,Y)(5) particles precipitation and realize interfacial bonding between chips. After peak-aged treatment at 225 degrees C, the GW102K alloy consolidated at 450 degrees C exhibits excellent tensile strength. It is feasible to obtain the desired tensile properties of the recycled alloys by simultaneously promoting the precipitation of the metastable RE-containing phases and retaining the work-hardening effect during heat treatment. (C) 2010 Elsevier B.V. All rights reserved.

关键词： Rare earth (RE) Mg-10Gd-2Y-0.5Zr Solid-state recycling Aging response Machined chips Interface bonding

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New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm

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TRANSGENIC research 2010年第1期19卷 29-44页

作者： Zhao, Aichun Zhao, Tianfu Zhang, Yuansong Xia, Qingyou Lu, Cheng Zhou, Zeyang Xiang, Zhonghuai Nakagaki, M. Southwest Univ Inst Sericulture & Syst Biol Minist Agr Key Sericultural Lab Chongqing 400716 Peoples R China Shinshu Univ Fac Text Sci & Technol Dept Appl Biol Ueda Nagano 3868567 Japan Southwest Univ Coll Text & Garment Chongqing 400716 Peoples R China

We constructed three different fibroin H-chain expression systems to estimate the efficacy of producing recombinant proteins in the cocoon of transgenic silkworms. The results showed that the three different EGFP/H-chain fusion genes were all expressed selectively in the posterior silk gland of the transgenic silkworm. The recombinant protein content of transgenic silkworm cocoons is up to 15% (w/w) when using the most highly efficient H-chain expression system. To our knowledge, in comparison with silkworm silk gland expression systems in the literature, the highly efficient expression system developed in this study is the most efficient silkworm silk gland expression system to date. This expression system is the best candidate for foreign gene production and for creation of novel functional silk material. The results suggested the N-terminal domain and the intron of the H-chain gene are important in the secretion of fibroin and its transcription, respectively.

关键词： Transgenic silkworms Fibroin heavy chain Bombyx mori Silk gland expression system piggyBac

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Genetic variation in apolipoprotein E alters regional gray matter volumes in remitted late-onset depression

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JOURNAL OF AFFECTIVE DISORDERS 2010年第3期121卷 273-277页

作者： Yuan, Yonggui Zhang, Zhijun Bai, Feng You, Jiayong Yui, Hui Shi, Yongmei Liu, Wen Southeast Univ Sch Clin Med Nanjing 210009 Peoples R China Nanjing Med Univ Nanjing Brain Hosp Dept Psychiat Nanjing 210029 Peoples R China Southeast Univ ZhongDa Hosp Dept Neurol Nanjing 210009 Peoples R China

Background The apolipoprotein E (ApoE) gene has been confirmed as the major genetic risk factor for late-onset Alzheimer's disease (AD) The effect of ApoE polymorphism on brain morphology still remains unclear in remains late-onset depression (RLOD) Methods A total of 37 patients with remitted geriatric depression were investigated with optimized voxel-based morphometry. We tested for differences in gray matter volume between ApoE epsilon 4 allele noncarriers (n = 25) and ApoE 1:4 allele carriers (n = 12) in RLOD patients Results. The volumes of right medial frontal gyrus, left middle frontal gyrus and left inferior occipital gyrus were significantly smaller in RLOD patients with ApoE 1:4 allele carriers as compared to ApoE epsilon 4 allele noncarners There was a significant positive correlation between gray matter volume of right medial frontal gyrus and Digit Span Test score in RLOD patients with ApoE epsilon 4 allele carriers. Limitations This study is cross-sectional. therefore it cannot determine whether abnormal gray matter volume is a state marker or trait market of RLOD with ApoE epsilon 4 allele carriers Conclusion Our findings support the hypothesis that the ApoE genotype might be associated with structural changes in RLOD. Crown Copyright (C) 2009 Published by Elseviei B.V All rights reserved

关键词： Remitted late-onset depression Apolipoprotein E Magnetic resonance imaging Voxel-based morphometry

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Adolescent escitalopram administration modifies neurochemical alterations in the hippocampus of maternally separated rats

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EUROPEAN NEUROPSYCHOPHARMACOLOGY 2010年第12期20卷 875-883页

作者： Hui, Jiaojie Zhang, Zhijun Liu, Shanshan Xi, Guangjun Zhang, Xiangrong Teng, GaoJun Chan, Kevin C. Wu, Ed X. Nie, Binbin Shan, Baoci Li, Lingjiang Reynolds, Gavin P. Southeast Univ Dept Neurol Affiliated ZhongDa Hosp Nanjing Jiangsu Peoples R China Southeast Univ Sch Clin Med Nanjing Jiangsu Peoples R China Southeast Univ Inst Mol Imaging Nanjing Jiangsu Peoples R China Univ Hong Kong Lab Biomed Imaging & Signal Proc Pokfulam Hong Kong Peoples R China Univ Hong Kong Dept Elect & Elect Engn Pokfulam Hong Kong Peoples R China Zhengzhou Univ Phys Sci & Technol Coll Zhengzhou Henan Peoples R China Chinese Acad Sci Inst High Energy Phys Key Lab Nucl Analyt Tech Beijing Peoples R China Cent S Univ Xiangya Hosp 2 Mental Hlth Inst Changsha Hunan Peoples R China Queens Univ Belfast Dept Psychiat Belfast BT9 7BL Antrim North Ireland

Early life stress is a potential precursor of eventual neuropsychiatric diseases and may result in altered neurodevelopment and function of the hippocampus, which thus provides a site at which potential interventions to modify the effects of early life stress may act. In this study, Sprague Dawley rat pups comprising male and female animals underwent maternal separation (MS) for 180 min from postnatal days (PND) 2 to 14, or were left with their dams. They subsequently received daily administration of saline (0.9%), escitalopram (10 mg/kg), or no treatment during adolescence (PND 43-60). All adult animals underwent brain magnetic resonance imaging (MRI) and bilateral hippocampal proton magnetic resonance spectroscopy (H-1-MRS). Neither MS nor escitalopram treatment had a significant effect on hippocampal volume. Adult rats that experienced MS displayed significantly increased choline-containing compounds (Cho) and decreased N-acetylaspartate (NAA), glutamate (Glu) and Myo-inositol (MI) relative to the stable neurometabolite creatine (Cr) in hippocampus. Administration of escitalopram during adolescence could modify the alterations of NAA/Cr, Glu/Cr and MI/Cr. The effects of MS on hippocampal neurochemistry were most significant in the right hippocampus. These results indicate that MS in rats has long-term consequences on hippocampal neurochemistry reflective of neural density/functional integrity, especially on the right hippocampus, and adolescent administration with escitalopram can at least partially ameliorate these neurochemical alterations. Furthermore, these metabolite changes seem to be more sensitive indicators of the results from early life stress than volume changes. (C) 2010 Elsevier B.V. and ECNP. All rights reserved

关键词： hippocampus Maternal separation Escitalopram Magnetic resonance imaging Proton magnetic resonance spectroscopy Rat

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highly efficient dynamic kinetic resolution of secondary aromatic alcohols with low-cost and easily available acid resins as racemization catalysts

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TETRAHEDRON LETTERS 2010年第17期51卷 2366-2369页

作者： Cheng, Yongmei Xu, Gang Wu, Jianping Zhang, Chensheng Yang, Lirong Zhejiang Univ Inst Bioengn Dept Biol & Chem Engn Hangzhou 310027 Zhejiang Peoples R China

A new and efficient dynamic kinetic resolution (DKR) process of secondary aromatic alcohols was developed with acid resins as racemization catalysts. Acid resin CD8604 was shown to have excellent racemization activity and good biocompatibility. When employing CD8604 and complex acyl donors as racemization catalyst and acyl donor, respectively, enantiomerically pure aromatic acetate was obtained with excellent yield and ee values through the DKR process. It is noteworthy that the system could be reused more than 10 times with little loss of yield and ee value. (C) 2010 Elsevier Ltd. All rights reserved.

关键词： ENZYMATIC RESOLUTION RUTHENIUM

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