检索结果-内蒙古大学图书馆

Overexpression of GRP78 enhances survival of CHO cells in response to serum deprivation and oxidative stress

ENGINEERING IN LIFE SCIENCES 2017年第2期17卷 107-116页

作者： Jiang, Qing Sun, Yuanli Guo, Zilong Fu, Mingpeng Wang, Qiang Zhu, Huifen Lei, Ping Shen, Guanxin Huazhong Univ Sci & Technol Tongji Med Coll Sch Basic Med Dept Immunol Wuhan Hubei Peoples R China Wuhan Univ Sci & Technol Coll Med Dept Immunol Wuhan Hubei Peoples R China

Chinese hamster ovary (CHO) cells are regarded as one of the most commonly used mammalian hosts, which decreases the productivity due to loss in culture viability. Overexpressing antiapoptosis genes in CHO cells was developed as a means of limiting cell death upon exposure to environmental insults. Glucose- regulated protein 78 (GRP78) is traditionally regarded as a major ER chaperone that participates in protein folding and other cell processes. It is also a potent antiapoptotic protein and plays a critical role in cell survival, proliferation, and metastasis. In this study, the impact of GRP78 on CHO cells in response to environmental insults such as serum deprivation and oxidative stress was investigated. First, it was confirmed that CHO cells were very sensitive to environmental insults. Then, GRP78 overexpressing CHO cell line was established and exposed to serum deprivation and H2O2. Results showed that GRP78 engineering increased the viability and decreased the apoptosis of CHO cells. The survival advantage due to GRP78 engineering could be mediated by suppression of caspase- 3 involved in cell death pathways in stressed cells. Besides, GRP78 engineering also enhanced yields of antibody against transferrin receptor in CHO cells. GRP78 should be a potential application in the biopharmaceutical industries.

关键词： Apoptosis ER stress Glucose-regulated protein 78 Oxidative stress Serum deprivation

来源：评论

学校读者我要写书评

暂无评论

Vehicle traffic delay prediction in ferry terminal based on Bayesian multiple models combination method

引用

TRANSPORTMETRICA A-TRANSPORT SCIENCE 2017年第5期13卷 467-490页

作者： Zhang, Weibin Qi, Yong Henrickson, Kristian Tang, Jinjun Wang, Yinhai Univ Washington Dept Civil & Environm Engn Smart Transportat Applicat & Res Lab Seattle WA 98195 USA Nanjing Univ Sci & Technol Nanjing 210094 Jiangsu Peoples R China Cent S Univ Sch Traff & Transportat Engn Changsha 410075 Hunan Peoples R China

This paper presents a new class of models for predicting vehicle traffic delay in ferry terminals. Ferry service plays an important role in many cities adjacent to navigable bodies of water. Transportation agencies, port authorities, and drivers can benefit from a reliable method for ferry traffic delay prediction, which can support improved ferry service scheduling, land-based transit and ferry coordination, and proactive trip planning. In this study, vehicle traffic delay within a ferry terminal is considered to be comprised of both periodic and dynamic components. Frequency analysis is applied to confirm the presence of a consistent periodic trend. A combination method is adopted to fit the dynamic component, which assembles artificial neural network, support vector machine, and ARIMA model in a Bayesian framework. The results presented indicate that, by aggregating the output of multiple models, this model ensemble approach can lead to greater predictive accuracy in modelling ferry traffic delay.

关键词： Ferry service traffic delay traffic prediction models ensemble Bayesian combination method

来源：评论

学校读者我要写书评

暂无评论

Microwave Staring Correlated Imaging Based on 3-Dimensinal Randomly Distributed Antenna Array 5

Microwave Staring Correlated Imaging Based on 3-Dimensinal R...

引用

5th IEEE Microwaves, Radar and Remote Sensing Symposium (MRRS)

作者： Guo, Yuanyue Li, Hongmin Liu, Falin Wang, Dongjin Univ Sci & Technol China Key Lab Electromagnet Space Informat Chinese Acad Sci Hefei Anhui Peoples R China

ISBN: (纸本)9781509053919

In this paper, we demonstrate a new three dimensional (3D) randomly distributed antenna array for microwave staring correlated imaging. Its construction method is presented and the imaging model based on it is established;In order to characterize its random spatial arrangement of array antennas quantitatively, 3D random distributed entropy is defined;Based on the optimal model of maximizing the distribution entropy, optimal random distribution of 3D antenna array can be obtained by using genetic algorithm. Numerical simulation confirms its validity of the proposed 3D antenna array in improving its stochastic characteristics of radiation fields and achieving better imaging results.

关键词： microwave staring correlated imaging 3D randomly distributed antenna array 3D random distributed entropy genetic algorithm

来源：评论

学校读者我要写书评

暂无评论

Global optimal energy management control strategies for connected four-wheel-drive hybrid electric vehicles

引用

IET INTELLIGENT TRANSPORT SYSTEMS 2017年第5期11卷 264-272页

作者： Qiu, Lihong Qian, Lijun Zomorodi, Hesam Pisu, Pierluigi Hefei Univ Technol Dept Vehicle Engn Hefei 230009 Peoples R China Clemson Univ Int Ctr Automot Res Greenville SC 29607 USA

This study presents a novel decentralised hierarchical global energy management control strategy for a group of connected four-wheel-drive hybrid electric vehicles (HEVs) in urban road conditions. In the higher level controller, signal phase and timing information and the optimal cruising velocity are combined to generate the target velocities for the HEVs. A model predictive control framework that focuses on the tracking of the target velocity and the associated desired control variable for every individual vehicle is proposed for the prediction of the optimal velocity that compromises fuel economy, mobility and safety. In the lower level controller, a dynamic programming problem is formulated that utilises the predicted velocity for the global energy management optimisation of every individual HEV. Simulation results validate the advantages of the proposed higher and lower level controllers.

关键词： energy management systems hybrid electric vehicles wheels predictive control fuel economy dynamic programming level control optimal control global optimal energy management control strategy connected four-wheel-drive hybrid electric vehicles decentralised hierarchical global energy management control strategy urban road conditions level controller timing information optimal cruising velocity target velocity generation HEVs model predictive control framework fuel economy dynamic programming problem global energy management optimisation

来源：评论

学校读者我要写书评

暂无评论

The catalytic performances and reaction mechanism of nanoparticle Cd/Ce-Ti oxide catalysts for NH₃-SCR reaction

引用

RSC ADVANCES 2017年第79期7卷 50127-50134页

作者： Duan, Zhichen Chi, Kebin Liu, Jian Shi, Juan Zhao, Zhen Wei, Yuechang Song, Weiyu China Univ Petr State Key Lab Heavy Oil Proc Beijing Key Lab Oil & Gas Pollut Control 18 Fuxue Rd Beijing 102249 Peoples R China Petro China Co Ltd Petrochem Res Inst Beijing 100195 Peoples R China

Ce-0.3-TiOx nanoparticle carrier was prepared by the sol-gel method, and a series of Cd-Ce-Ti nanoparticle catalysts with variable Cd contents were prepared by the means of an improved incipientwetness impregnation. The catalysts were characterized by means of XRD, N-2 adsorption-desorption analysis, SEM, TEM, NH3-TPD and in situ DRIFTS. The catalytic activities for deNOx were evaluated by NH3-SCR reaction. All these nanoparticle catalysts contain mesopores with a pore size around 7 nm, and the average particle size is 20 nm. Among the catalysts, 2 wt% Cd/Ce0.3TiOx catalyst exhibits the best NH3-SCR performance with a wide temperature window of 250-400 degrees C for NO conversion above 90%. Moreover, in situ DRIFTS spectra of NOx reduction over 2 wt% Cd/Ce0.3TiOx catalyst were also investigated. The results show that this reaction mainly follows the Langmuir-hinshelwood mechanism at room temperature, while Eley-Rideal mechanism plays more important role when the reaction temperature is higher than 150 degrees C. The adsorbed NH3 coordinately linked to Lewis acid site is easy to react with NOx at high temperature.

关键词： Catalyst activity

来源：评论

学校读者我要写书评

暂无评论

Diversity analysis and quantification of lactic acid bacteria in traditionally fermented yaks' milk products from Tibet

引用

FOOD BIOtechNOLOGY 2017年第1期31卷 1-19页

作者： Ren, Yan Yang, Yanrong Zhang, Donglei Wang, Dan Zhang, Heping Liu, Wenjun Inner Mongolia Agr Univ Key Lab Dairy Biotechnol & Engn Minist Educ Hohhot 010018 Peoples R China

Fermented yaks' milk is a characteristic fermented dairy product with a rich microflora (especially lactic acid bacteria [LAB]), that has been produced by Tibetan people in china throughout history. Systematic analysis of the LAB composition of fermented yaks' milk from central Tibet is essential. In the present study, 38 samples of fermented yaks' milk were collected from three regions in central Tibet. From these, a total of 211 isolates of LAB were generated using traditional pure culture methods. To accurately analyze their biodiversity, 16S rRNA gene sequence analysis and denaturing gradient gel electrophoresis (DGGE) were used. The isolates were identified as belonging to six genera and 22 species/subspecies. Among them, Lactobacillus species predominated (117 isolates) accounting for 55.45% of all isolates. Overall, L. delbrueckii subsp. bulgaricus (60 isolates;28.43% of the total) was the most commonly isolated species of LAB from fermented yaks' milk from central Tibet. Seven Lactobacillus species and the Bifidobacterium genus were confirmed by quantitative PCR (q-PCR). The q-PCR results showed that the abundance of these groups in fermented yaks' milk from central Tibet ranged from 1.12 +/- 0.36 log cfu/ml (for L. sakei from Ningzhong) to 7.77 +/- 0.84 log cfu/ml (for L. helvetivus from Ningzhong). Lactobacillus acidophilus, L. delbrueckii subsp. bulgaricus, L. fermentum, L. helveticus varied in concentration depending on the region. The results indicated that traditional fermented yaks' milk from different regions of central Tibet have complex compositions of LAB species. The diversity of LAB might be related to geographical origin.

关键词： Lactic acid bacteria Fermented yaks' milk 16S rRNA gene sequencing denaturing gradient gel electrophoresis (DGGE) analysis quantitative PCR

来源：评论

学校读者我要写书评

暂无评论

Audio Feature Learning with Triplet-Based Embedding Network 31

Audio Feature Learning with Triplet-Based Embedding Network

引用

31st AAAI Conference on Artificial Intelligence

作者： Qi, Xiaoyu Yang, Deshun Chen, Xiaoou Peking Univ Inst Comp Sci & Technol 128th ZhongGuanCun North St Beijing 100080 Peoples R China

We propose a triplet-based network for audio feature learning for version identification. Existing methods use hand-crafted features for a music as a whole while we learn features by a triplet-based neural network on segment-level, focusing on the most similar parts between music versions. We conduct extensive experiments and demonstrate our merits.

关键词： Music

来源：评论

学校读者我要写书评

暂无评论

Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts

引用

BIOtechNOLOGY FOR BIOFUELS 2018年第1期11卷 277-277页

作者： Wang, Laiyou Deng, Aihua Zhang, Yun Liu, Shuwen Liang, Yong Bai, Hua Cui, Di Qiu, Qidi Shang, Xiuling Yang, Zhao He, Xiuping Wen, Tingyi Chinese Acad Sci Inst Microbiol CAS Key Lab Pathogen Microbiol & Immunol Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Chinese Acad Sci Inst Microbiol CAS Key Lab Microbial Physiol & Metab Engn Beijing 100101 Peoples R China Univ Chinese Acad Sci Savaid Med Sch Beijing 100049 Peoples R China

Background: The thermotolerant methylotrophic yeast Ogataea polymorpha has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized as an efficient and safe cell factory in fermentative productions of chemicals, biofuels and other bio-products. However, it is difficult to genetically engineer for the deficiency of an efficient and versatile genome editing technology. Results: In this study, we developed a CRISPR-Cas9-assisted multiplex genome editing (CMGE) approach including multiplex genes knock-outs, multi-locus (ML) and multi-copy (MC) integration methods in yeasts. Based on CMGE, various genome modifications, including gene deletion, integration, and precise point mutation, were performed in O. polymorpha. Using the CMGE-ML integration method, three genes TAL from Herpetosiphon aurantiacus, 4CL from Arabidopsis thaliana and STS from Vitis vinifera of resveratrol biosynthetic pathway were simultaneously integrated at three different loci, firstly achieving the biosynthesis of resveratrol in O. polymorpha. Using the CMGE-MC method,similar to 10 copies of the fusion expression cassette P-ScTEF1-TAL-P-ScTPI1-4CL-P-ScTEF2-STS were integrated into the genome. Resveratrol production was increased similar to 20 fold compared to the one copy integrant and reached 97.23 +/- 4.84 mg/L. Moreover, the biosynthesis of human serum albumin and cadaverine were achieved in O. polymorpha using CMGE-MC to integrate genes HSA and cadA, respectively. In addition, the CMGE-MC method was successfully developed in Saccharomyces cerevisiae. Conclusions: An efficient and versatile multiplex genome editing method was developed in yeasts. The method would provide an efficient toolkit for genetic engineering and synthetic biology researches of O. polymorpha and other yeast species.

关键词： CRISPR-Cas9-assisted multiplex genome editing Markerless multi-locus integration Markerless multi-copy integration Ogataea polymorpha Saccharomyces cerevisiae

来源：评论

学校读者我要写书评

暂无评论

A method of emission and traveller behavior analysis under multimodal traffic condition

引用

TRANSPORTATION research PART D-TRANSPORT AND ENVIRONMENT 2017年第PartA期52卷 139-155页

作者： Zhang, Weibin Qi, Yong Yan, Ying Tang, Jinjun Wang, Yinhai Univ Washington Dept Civil & Environm Engn Smart Transportat Applicat & Res Lab Seattle WA 98195 USA Nanjing Univ Sci & Technol Sch Comp Sci & Engn Nanjing 210094 Jiangsu Peoples R China Changan Univ Automobile Sch Key Lab Transportat Safety Support Technol Xian 710064 Shaanxi Peoples R China Cent S Univ Sch Traff & Transportat Engn Changsha 410075 Hunan Peoples R China

The growth of urban traffic in recent decades has led to environmental problems in terms of increased emissions. In some cities located along waterfronts, multimodal transportation that includes ferry trips plays an important role in public transportation. Compared with roadway traffic, ferry service can often provide shorter routes between given origins and destinations as well as reduce emissions. However, in some areas with high ferry demand, travel delay occurs frequently because of traffic congestion caused by drivers waiting to board the ferry, which can significantly increase travel time and decrease its reliability. In addition, changes in other factors, such as fuel prices, also may influence drivers' decision making processes in terms of route choice. In this paper, a study focusing on travel time and travel time reliability of ferry service during peak travel periods is performed. Furthermore, as a key result an emission loss model under multimodal conditions is developed that also takes travel cost into account. The analysis of route choice is conducted based on a mixed logit model which is able to handle individual preference. Based upon validation with data from the Seattle area, the proposed model is verified as an efficient way to analyse emission factors in a multimodal transportation environment. (C) 2017 Elsevier Ltd. All rights reserved.

关键词： Emission Ferry traffic Route choice Travel time reliability Multimodal transportation

来源：评论

学校读者我要写书评

暂无评论

Abnormal n-p-n type conductivity transition of hollow ZnO/ZnFe₂O₄ nanostructures during gas sensing process: The role of ZnO-ZnFe₂O₄ hetero-interface

引用

SENSORS AND ACTUATORS B-CHEMICAL 2017年 253卷 144-155页

作者： Li, Wenhui Wu, Xiaofeng Chen, Jiayuan Gong, Yan Han, Ning Chen, Yunfa Chinese Acad Sci Inst Proc Engn State Key Lab Multiphase Complex Syst Beijing 100190 Peoples R China Univ Chinese Acad Sci 19A Yuquan Rd Beijing 100049 Peoples R China

The hollow ZnO/ZnFe2O4 microspheres with heterogeneous structure are synthesized by direct pyrolysis of metal-organic frameworks. The as-prepared ZnO/ZnFe2O4 microspheres have well-defined spherical morphology with 1.5 pin in diameter and multiple porous shells constructed by interpenetrated ZnO and ZnFe2O4 heterogeneous nanoparticles. Interestingly, the hollow ZnO/ZnFe2O4 microspheres based gas sensors show interestingly temperature-dependent n-p-n type conductivity transition in detecting low-concentration VOC gases including ethanol, acetone, toluene and benzene. This interestingly n-p-n transition phenomenon is mainly ascribed to the trade-off of highly separated electron-hole pairs originated from the staggered type-II band alignment of in-shell ZnO-ZnFe2O4 hetero-interfaces, which is modulated by thermally-dependent ionization reaction of surface-absorbed oxygen molecules and extra electron injection due to surface reaction of reductive VOCs during gas-sensing process. This work presents a facile route to construct hollow nanostructures with heterogeneous feature and provides a new insight into sensing mechanism, exhibiting the potential application of ZnO/ZnFe2O4 microspheres in developing highly sensitive and selective gas-sensing materials in detecting low-concentration VOCs. (C) 2017 Elsevier B.V. All rights reserved.

关键词： ZnO/ZnFe2O4 Hollow microspheres Gas sensor p-n conducting transition

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：