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Deep Learning-Assisted Label-Free Parallel Cell Sorting with Digital Microfluidics

advanced SCIENCE 2025年第1期12卷 e2408353页

作者： Guo, Zongliang Li, Fenggang Li, Hang Zhao, Menglei Liu, Haobing Wang, Haopu Hu, Hanqi Fu, Rongxin Lu, Yao Hu, Siyi Xie, Huikai Ma, Hanbin Zhang, Shuailong Beijing Inst Technol Beijing Adv Innovat Ctr Intelligent Robots & Syst Sch Mechatron Engn Beijing 100081 Peoples R China Beijing Inst Technol Sch Med Technol Beijing 100081 Peoples R China Beijing Inst Technol Engn Res Ctr Integrated Acousto Optoelect Microsys Sch Integrated Circuits & Elect Minist Educ China Beijing 100081 Peoples R China Chinese Acad Sci Suzhou Inst Biomed Engn & Technol CAS Key Lab Biomed Diagnost Suzhou 215163 Peoples R China ACX Instruments Ltd St Johns Innovat Ctr Cambridge CB40WS England

Sorting specific cells from heterogeneous samples is important for research and clinical applications. In this work, a novel label-free cell sorting method is presented that integrates deep learning image recognition with microfluidic manipulation to differentiate cells based on morphology. Using an Active-Matrix Digital Microfluidics (AM-DMF) platform, the YOLOv8 object detection model ensures precise droplet classification, and the Safe Interval Path Planning algorithm manages multi-target, collision-free droplet path planning. Simulations and experiments revealed that detection model precision, concentration ratios, and sorting cycles significantly affect recovery rates and purity. With HeLa cells and polystyrene beads as samples, the method achieved 98.5% sorting precision, 96.49% purity, and an 80% recovery over three cycles. After a series of experimental validations, this method can also be used to sort HeLa cells from red blood cells, cancer cells from white blood cells (represented by HeLa and Jurkat cells), and differentiate white blood cell subtypes (represented by HL-60 cells and Jurkat cells). Cells sorted using this method can be lysed directly on chip within their hosting droplets, ensuring minimal sample loss and suitability for downstream bioanalysis. This innovative AM-DMF cell sorting technique holds significant potential to advance diagnostics, therapeutics, and fundamental research in cell biology.

关键词： artificial intelligence cell sorting digital microfluidics droplet single cell research label-free

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Interactive Virtual Ankle Movement Controlled by Wrist sEMG Improves Motor Imagery: An Exploratory Study

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IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS 2024年第8期30卷 5507-5524页

作者： Xiao, Yanqing Bai, Hongming Gao, Yang Hu, Ben Zheng, Jia Cai, Xiaoe Rao, Jiasheng Li, Xiaoguang Hao, Aimin Beihang Univ Beijing Adv Innovat Ctr Biomed Engn Sch Biol Sci & Med Engn Beijing Key Lab Biomat & Neural Regenerat Beijing 100191 Peoples R China Beihang Univ State Key Lab Virtual Real Technol & Syst Beijing 100191 Peoples R China Chinese Acad Med Sci Res Unit Virtual Body & Virtual Surg 2019RU004 Beijing 100050 Peoples R China Capital Med Univ Beijing Childrens Hosp Beijing 100045 Peoples R China Beijing Haidian Hosp Dept Rehabil Med Beijing 100080 Peoples R China Capital Med Univ Sch Basic Med Sci Dept Neurobiol Beijing 100069 Peoples R China

Virtual reality (VR) techniques can significantly enhance motor imagery training by creating a strong illusion of action for central sensory stimulation. In this article, we establish a precedent by using surface electromyography (sEMG) of contralateral wrist movement to trigger virtual ankle movement through an improved data-driven approach with a continuous sEMG signal for fast and accurate intention recognition. Our developed VR interactive system can provide feedback training for stroke patients in the early stages, even if there is no active ankle movement. Our objectives are to evaluate: 1) the effects of VR immersion mode on body illusion, kinesthetic illusion, and motor imagery performance in stroke patients;2) the effects of motivation and attention when utilizing wrist sEMG as a trigger signal for virtual ankle motion;3) the acute effects on motor function in stroke patients. Through a series of well-designed experiments, we have found that, compared to the 2D condition, VR significantly increases the degree of kinesthetic illusion and body ownership of the patients, and improves their motor imagery performance and motor memory. When compared to conditions without feedback, using contralateral wrist sEMG signals as trigger signals for virtual ankle movement enhances patients' sustained attention and motivation during repetitive tasks. Furthermore, the combination of VR and feedback has an acute impact on motor function. Our exploratory study suggests that the sEMG-based immersive virtual interactive feedback provides an effective option for active rehabilitation training for severe hemiplegia patients in the early stages, with great potential for clinical application.

关键词： Training Stroke (medical condition) Wrist Muscles Legged locomotion Biological system modeling Real-time systems VR-based stroke rehabilitation training motor imagery sEMG-based virtual feedback

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High-temperature wear behaviour of plasma cladded WC/Ni60A composite coating on copper

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MATERIALS SCIENCE AND technology 2023年第4期39卷 403-411页

作者： Zhang, Jun Hu, Rong Wang, Ruidong Bai, Dan Zhang, Chao Zhang, Yuwen Wu, Wenhe Li, Guangshi Yang, Yuzhu Liu, Quanli Lu, Xionggang Shanghai Univ State Key Lab Adv Special Steel Shanghai 200444 Peoples R China Shanghai Univ Shanghai Key Lab Adv Ferromet Shanghai 200444 Peoples R China Shanghai Univ Sch Mat Sci & Engn Shanghai 200444 Peoples R China Changli Xingguo Precis Machinery Co Ltd Changli Peoples R China

With the aim of improving the surface properties of copper, WC/Ni60A composite coatings were successfully prepared by plasma cladding. A good metallurgical bonding was formed between composite coating and copper substrate. The large amount of dispersed WC and W2C phases in the coating significantly increased the microhardness (17 times that of the copper substrate). With the rise in temperature, the wear resistance of the coating first increased and then decreased. At 400 degrees C, the coating had the best wear resistance due to the lubricating effect of the oxide layer. However, due to the simultaneous occurrence of various wear mechanisms, the wear resistance at 600 degrees C was significantly reduced, so the service temperature must be controlled below 600 degrees C.

关键词： Copper pipe plasma cladding WC Ni60A microstructure high-temperature wear behaviour

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Molecular Dynamics Study of GaN Thin Films Deposited on Non-polar m-plane of A1N Substrate 25

Molecular Dynamics Study of GaN Thin Films Deposited on Non-...

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25th International Conference on Electronic Packaging technology (ICEPT)

作者： Li, Rui Wang, Shizhao Wu, Gai Sun, Xiang Liang, Kang Liu, Sheng Wuhan Univ Sch Power & Mech Engn Wuhan Peoples R China IC Res Technol Wuhan Co Ltd Wuhan Peoples R China Wuhan Univ Inst Technol Sci Wuhan Peoples R China

ISBN: (纸本)9798350353808

The GaN deposited on polar surfaces exhibits significant spontaneous polarization effects, which can cause Quantum Confinement Stark Effect. However, growing on non-polar surfaces can avoid these issues. This study used molecular dynamics method to investigate the epitaxial growth of GaN thin film on non-polar m-plane (1-100) at different temperatures. The variation of the surface morphology and the crystalline structure of simulation system were discussed in detail. The simulation results showed that as the epitaxial temperature rises, the mobility of surface atoms along [0001] direction was significantly higher than that along [11-20] direction, so that the small island structure on the surface were connected along [0001] direction. In addition, the increase in temperature was beneficial for improving the atomic migration ability, which leads to the number of wurtzite atoms increases and the number of amorphous atoms decreases.

关键词： Molecular dynamics GaN m-plane Epitaxial growth Different temperatures

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Analysis of physiological and biochemical changes and metabolic shifts during 21-Day fasting hypometabolism

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SCIENTIFIC REPORTS 2024年第1期14卷 1-13页

作者： Dai, Zhongquan Zhang, Hongyu Sui, Xiukun Wu, Feng Zhang, Cheng Fan, Zhiqi Wang, Hailong Guo, Yaxiu Yang, Chao Jiang, Siyu Wang, Linjie Xin, Bingmu Li, Yinghui Astronaut Res & Training Ctr State Key Lab Space Med Beijing 100094 Peoples R China Space Sci & Technol Inst Engn Res Ctr Human Circadian Rhythm & Sleep Shenzhen 518117 Peoples R China

This study aimed to evaluate the impact of prolonged fasting on the physiological and biochemical alterations and metabolic shifts in healthy adults and to provide experimental data and theoretical support for the hypometabolic state induced by prolonged fasting. Thirteen volunteers were selected through public recruitment to undergo a 21-day complete fasting experiment. The experimental period lasted 34 days, including a 3-day baseline, 21-day completing fasting, 5-day calorie restriction and 5-day full recovery diet. Physiological indicators such as body weight, blood pressure, blood glucose, blood ketones, and blood uric acid were evaluated along with resting metabolic rate, routine blood tests, liver function, and heart function indexes employing traditional approaches. During the 21-day complete fasting period, there was a significant decrease in body weight (average - 14.96 +/- 1.55%), a reduction in blood glucose (average - 21.63 +/- 0.058%), an increase in blood ketones (from baseline 0.1 +/- 0.04 mmol/L to 6.61 +/- 1.25 mmol/L) and blood uric acid (from baseline 385.38 +/- 57.78 mu mol/L to 866.31 +/- 172.01 mu mol/L), a continuous decline in resting energy expenditure (average - 20.3 +/- 11.13%), and the respiratory quotient tending towards fat metabolism. Most of the items in the complete blood count and liver indicators remained stable and within the normal range. Heart function showed functional adaptive changes without structural damage. Prolonged fasting can reduce the body's resting energy expenditure and adapt to body weight loss through physiological regulatory mechanisms without adverse effects on basic physiological functions or the structure of important organs. Under medical supervision, healthy adults can safely engage in prolonged fasting for up to 21 days with metabolic adaption and no damage to pivotal organ, which could provide potential technical support for human health and survival strategies in extreme conditions such as food shortages

关键词： Prolonged fasting Survival protection techniques Hypometabolism, physiological safety Energy expenditure Manned spaceflight

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An in-situ synthesised TiC/Ni60A composite coating on copper by plasma cladding

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MATERIALS SCIENCE AND technology 2023年第6期39卷 729-735页

作者： Hu, Rong Wang, Ruidong Zhang, Jun Zhang, Chao Zhang, Yuwen Li, Guangshi Lu, Xionggang Yang, Yuzhu Liu, Quanli Shanghai Univ State Key Lab Adv Special Steel Shanghai 200444 Peoples R China Shanghai Univ Shanghai Key Lab Adv Ferromet Shanghai 200444 Peoples R China Shanghai Univ Sch Mat Sci & Engn Shanghai 200444 Peoples R China Changli Xingguo Precis Machinery Co Ltd Changli Peoples R China

The in-situ synthesis of TiC reinforced Ni-based coatings were prepared on copper substrates by using precursor materials (TiFe and Cr3C2) by plasma cladding. The coating consisted mainly of gamma-(Cu, Fe, Ni), TiC, CrB and Cr23C6. The generated TiC particles tend to float up to the upper part of the coating to aggregate and grow, so the TiC concentration in the upper part is larger. The hardness of the TiC composite coating is distributed in a gradient, and the average hardness is 700 HV, which is 13.0 times that of the copper matrix. The wear mechanism of TiC composite coating is abrasive wear, and the wear resistance is 13.0 times that of the copper substrate.

关键词： In-situ coating TiC particles wear resistance

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Recent advances in dendrite-free lithium metal anodes for high-performance batteries

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PHYSICAL CHEMISTRY CHEMICAL PHYSICS 2022年第34期24卷 19996-20011页

作者： Zhang, Xiang Sun, Chunwen China Univ Min & Technol Beijing Sch Chem & Environm Engn Beijing 100083 Peoples R China

With the merits of high energy density, light weight, and low electrode potential, lithium metal anodes (LMAs) have lately sparked worldwide attention in the field of batteries. However, their low Coulombic efficiency, tremendous volume expansion, and serious dendrite growth make lithium metal batteries (LMBs) unsuitable for a wide variety of applications. Moreover, when lithium dendrite crosses the electrolyte and reaches the cathode material, it may cause short circuit and safety issues for batteries. Herein, to accelerate the development of LMBs, we give a brief summary of the dendrite growth mechanisms in both liquid and solid systems of electrolytes. In particular, various modification approaches to dendrite-free lithium metal batteries are discussed. Furthermore, advanced in situ characterization techniques for the real-time observation of lithium dendrite growth are presented. To address the application issues, various potential research routes for improving the performance of LMBs are provided as well.

关键词： Electrolytes

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Microstructures and Properties of AlCoCrNiFe High-Entropy Alloy Sintered by Hot Oscillating Pressing Under Different Sintering Times

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JOM 2024年第5期76卷 2554-2562页

作者： Gao, Ka Wu, Ziqian Ren, Chunyang Xiong, Yan Sun, Dejian Gao, Yang An, Linan Univ Shanghai Sci & Technol Sch Mech Engn Shanghai 200093 Peoples R China Chongqing Univ Technol Key Lab Adv Manufacture Technol Automobile Parts Minist Educ Chongqing 401135 Peoples R China Zhengzhou Univ Aeronaut Sch Mat Sci & Engn Zhengzhou 450015 Peoples R China Hubei Univ Technol Hubei Prov Key Lab Green Mat Light Ind Wuhan 430068 Peoples R China Dongguan Univ Technol Sch Mech Engn Dongguan 523808 Peoples R China

Powder metallurgy is an effective method for preparing high-performance powder high-entropy alloys (HEAs). Among them, the sintering time has an important effect on the microstructure, density, Vickers hardness, and corrosion resistance of AlCoCrNiFe HEAs sintered by hot oscillating pressing (HOP). Thus the effect of sintering time (0.5 h, 1 h, and 1.5 h) on the microstructure and properties of AlCoCrNiFe HEAs has been systematically investigated in this work. The results showed that the densities of HEAs alloys showed a linear relationship with the content of the FCC phase and sintering time. At the same sintering time, HOP could effectively promote the disappearance of the original particle boundary, improve the microstructure uniformity, and reduce the internal pores. The hardness and corrosion resistance properties were increased with increasing sintering time, and were better than those of the hot press (HP)-sintered samples. When the sintering time was 1.5 h, the density and hardness of a HOP-ed sample could reach 99.18% and 479.12 HV1, respectively, while the minimum corrosion current density and corrosion rate were only 6.5 mu A/cm2 and 0.055 mm/year, respectively. Thus, the hardness and corrosion resistance properties of AlCoCrNiFe had been simultaneously enhanced. Therefore, we believe that appropriately increasing the sintering time is beneficial for improving the performance of HOP-ed HEAs alloys, but this does not mean that the sintering time can be endlessly increased.

关键词： Iron alloys

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Deoxygenation-Promoted Aggregation-Induced TADF-RTP Dual Emission for High-Contrast Channel-Selectable Hypoxia Probing

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JOURNAL OF PHYSICAL CHEMISTRY C 2024年第22期128卷 9385-9392页

作者： Ye, Danfeng Zhou, Lulu Sahoo, Smruti Ranjan Baryshnikov, Glib V. Agren, Hans Zhu, Liangliang Ningbo Univ Technol Coll Mat Sci & Chem Engn Ningbo 315211 Peoples R China Fudan Univ Dept Macromol Sci State Key Lab Mol Engn Polymers Shanghai 200438 Peoples R China Linkoping Univ Dept Sci & Technol Lab Organ Elect SE-60174 Norrkoping Sweden Uppsala Univ Dept Phys & Astron SE-75120 Uppsala Sweden

Thermally activated delayed fluorescence (TADF) and room-temperature phosphorescence (RTP) are two highly applicable emissive processes due to their time-resolved characteristics and their ability to respond to external stimuli. They have recently been integrated for comprehensive use in some solid-state luminogens;however, there remains a lack of molecular systems that can integrate them in the solution aggregated state, which considerably limits relevant biological applications. In this study, we present a molecular design enabling aggregation-induced TADF-RTP dual emission in the solution aggregated state, relying on the coexistence of T-1-to-S-1 and T-1-to-S-0 electronic processes in push-pull structures. This dual emission feature constitutes a broad spectral band with full width at half-maximum up to 175 nm, providing the ability to select different channels for biological detection and imaging. Moreover, deoxygenation is shown to promote molecular aggregation, leading to a significantly strengthened dual emission that enables a high-contrast hypoxia probing effect. These results not only reveal new photophysical mechanisms on organic molecules but also expand the detection functionality of luminescent materials in the biological field.

关键词： Aggregation Biological imaging Fluorescence Luminescence Oxygen

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Rational Design of Near-Infrared Reflective Pigments by Adjusting the Ni²⁺ Coordination Environment

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INORGANIC CHEMISTRY 2025年第10期64卷 5241-5251页

作者： Wei, Jiawan Wu, Yi Sun, Kangzhen Li, Pengzhai Fang, Qiuli Zhang, Yin Nanjing Tech Univ Coll Mat Sci & Engn Nanjing 211816 Peoples R China

Inorganic pigments offer excellent potential because of their resilience in a variety of environments, and high-reflectance near-infrared (NIR) pigments are a useful tactic to reduce the heat island effect. Nonetheless, the design of inorganic pigments is still dominated by serendipity, and it has never been easy to come up with logical designs for NIR reflective inorganic pigments. By modifying the coordination environment of Ni2+ using the generic formulas LiMg1-x Ni x PO4 and LiZn1-x Ni x PO4 (x = 0.02-0.12), yellow and violet pigments were successfully developed in this research. According to structural investigations, Ni2+ effectively replaced Mg2+ and Zn2+ to create [NiO4] tetrahedra and [NiO6] octahedra, causing an orderly alteration of the cell parameters. The diverse transition modes of Ni2+ in various crystal fields are the cause of the noticeable color changes caused by the solid solutions, according to the UV-vis-NIR spectra. Furthermore, both pigments have outstanding NIR solar reflectivity and coloring capabilities. Both the sample-colored coatings and the coating's surface under infrared light demonstrated lower temperatures than the commercial pigmented coatings for thermal insulation applications.

关键词： Thermal insulation

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