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Respiratory Metabolism and Metabolomics of Red Swamp Crayfish Procambarus clarkii Under Low Temperature Stress

JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY 2025年第5期343卷 578-589页

作者： Ding, Yu Sha, Wenbin Sun, Yunfei Cheng, Yongxu Shanghai Ocean Univ Key Lab Integrated Rice Fish Farming Ecosyst Minist Agr & Rural Affairs Shanghai Peoples R China Shanghai Ocean Univ Key Lab Freshwater Aquat Genet Resources Minist Agr & Rural Affairs Shanghai Peoples R China Shanghai Ocean Univ Shanghai Aquaculture Engn & Technol Res Ctr Shanghai Peoples R China Shanghai Ocean Univ Natl Demonstrat Ctr Expt Fisheries Sci Educ Shanghai Peoples R China

In the integrated rice-crayfish aquaculture systems, crayfish Procambarus clarkii need to spend a long winter in the ring ditch, which is vulnerable to low temperature stress, especially in the northern part of china, where cold waves and other low-temperature climates are frequent. To study the metabolic response of P. clarkii to low temperature stress experimentally, the temperature was lowered from the control (23 degrees C) to the low temperature group (9 degrees C, 5 degrees C, and 1 degrees C) by slow and uniform cooling, and molecular and physiological samples were collected for measurement. The results showed that low temperature stress damaged the gill membrane and the epithelial layer of gill tissues, with an increase in vacuoles area and a reduced and irregular distribution of hemocytes. As the temperature decreased, the oxygen consumption rate, ammonia excretion rate and maximum metabolic rate of P. clarkii decreased gradually, the oxygen-nitrogen ratio decreased but still remained at a high level, and the metabolic energy supply substances were always mainly lipids and carbohydrates. The pyruvate kinase activity tended to increase with decreasing temperature under low temperature stress, while hexokinase, succinate dehydrogenase and lactate dehydrogenase activities decreased gradually. The 183 differential metabolites were screened in the low temperature group compared with the control mostly enriched in amino acid metabolism and citrate cycle metabolic pathways. In conclusion, under low temperature stress, the gill was damaged, respiratory metabolism decreased, and glycolysis was enhanced. Since the citrate cycle metabolism was suppressed, P. clarkii needed to resist low temperature stress by increasing the amino acid metabolism to provide more energy to maintain cellular activity. The results unraveled metabolic response mechanisms of metabolic response mechanism of P. clarkii to low temperature stress, and provided theoretical references for the sele

关键词： low temperature metabolomics Procambarus clarkii respiratory metabolic rate respiratory metabolism enzymes

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Multidimensional landscape changes contribute significantly to vegetation distribution: The evidences from the Luo River Watershed in the eastern part of the Qinling Mountains, china

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ENVIRONMENTAL MANAGEMENT 2025年 1-16页

作者： Wang, Jin Wang, Jinman Zhang, Jianing China Univ Geosci Sch Land Sci & Technol 29 Xueyuan Rd Beijing 100083 Peoples R China Minist Nat Resources Technol Innovat Ctr Ecol Restorat Min Areas Beijing 100035 Peoples R China

Assessment of vegetation characteristics plays an important role in monitoring ecosystem health and restoration status, and it is necessary to consider the response characteristics of vegetation assessment indicator (VAI) to multidimensional landscape structure. Therefore, this research constructed a framework for analyzing landscape structure from two dimensions of landscape element and landscape pattern, selected fractional vegetation cover (FVC) and net primary productivity (NPP) as VAI, determined the response degree and spatial correlation of VAI to landscape structure by using geographical detector and bivariate Moran's index, and finally obtained the change threshold of VAI based on the constraints of landscape pattern by building a cubic polynomial. FVC and NPP were significantly higher from 2001 to 2021, with the expansion of the high-value areas. Cropland and woodland were the dominant landscape elements. The spatial explanatory power of landscape element for VAI was better than that of landscape pattern characteristics, while the combined factors in landscape structure all explained VAI better than univariate. The response of FVC to landscape structure was more significant compared to NPP. The high value areas of FVC tended to occur in natural landscape elements. FVC showed spatial correlation and clustering characteristics with both PD and LPI. One or more thresholds existed in the response of FVC to changes in landscape pattern. The research results have important reference value in the optimization of landscape structure and vegetation restoration, and can provide practical guidance for the sustainable development of the ecosystem.

关键词： Landscape structure Fractional vegetation cover Net primary productivity Vegetation restoration Watershed

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Electrospun anisotropic fiber reinforced composites for artificial heart valves

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JOURNAL OF MATERIALS CHEMISTRY B 2025年第12期13卷 3918-3929页

作者： Yang, Xiuling Chen, Yifeng Wu, Qijun Yang, Haoqi Duan, Gaigai Fu, Qiliang He, Haonan Zhang, Peng Ji, Jian Jiang, Shaohua Nanjing Forestry Univ Coll Mat Sci & Engn Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo Int Innovat Ctr Forest Chem & Mat Nanjing 210037 Peoples R China Zhejiang Univ Dept Polymer Sci & Engn MOE Key Lab Macromol Synth & Functionalizat Hangzhou Peoples R China Yangzhou Univ Inst Technol Carbon Neutralizat Coll Elect Energy & Power Engn Yangzhou 225127 Jiangsu Peoples R China

Anisotropic composite valves that approximate natural heart valves are essential for the successful construction of tissue-engineered heart valves. In this work, anisotropic nylon (polyamides, PA) fiber membranes were prepared via electrospinning and further composited with thermoplastic polyurethane (TPU) by the impregnation method to obtain anisotropic PA/TPU composite valves. Young's modulus of the PA/TPU composite valves in the axial and radial directions along the fibers was 85.07 +/- 4.22 MPa and 28.72 +/- 1.16 MPa, respectively. The anisotropic PA/TPU composite valve exhibited excellent anisotropy, and its anisotropy ratio was 3.03, which was close to that of natural valves. Besides, the anisotropic PA/TPU composite valve exhibits high transparency (similar to 87%). The in vitro experiments revealed that anisotropic PA/TPU composite valves have better resistance to calcification and good blood compatibility compared with anisotropic nylon fiber membranes. Moreover, the hydrophilicity presented by the anisotropic PA/TPU composite valve reduced the adhesion of calcified particles. The good anticoagulant properties exhibited by the anisotropic PA/TPU composite valve effectively reduced the formation of thrombus after implantation. Cellular experiments and subcutaneous implantation experiments in rats showed that anisotropic PA/TPU composite valves exhibit cellular nontoxicity and good biocompatibility, contributing to cellular differentiation and growth. This preparation method has great potential in preparing anisotropic composite valves.

关键词： Rayon

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Positive Feedback Mechanism of Stability Degradation of the IrO2/TiO2 Electrocatalyst Induced by Inferior Catalytic Activity

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ENERGY & FUELS 2025年第3期39卷 1735-1745页

作者： Zhang, Zeping Yao, Muhan Huang, Renxing Sang, Chengcheng Mao, Zongqiang Wang, Yunan Yin, Yanan Wang, Cheng Tsinghua Univ Inst Nucl & New Energy Technol Beijing 100084 Peoples R China Hill Sch Pottstown PA 19464 USA Nanjing Univ Sch Phys Nanjing 210003 Peoples R China Hunan Univ Coll Mech & Vehicle Engn Changsha 410082 Peoples R China Chinese Acad Sci Ningbo Inst Mat Technol & Engn Ningbo 315201 Peoples R China

Iridium (Ir)-based catalysts embracing both catalytic activity and stability are of great significance for scaling up the application of proton exchange membrane water electrolysis (PEMWE). Herein, supported IrO2 on TiO2 (IrO2/TiO2) with high specific surface area and electrolytic activity was prepared by adopting a chlorine (Cl)-free synthesis based on the Adams fusion method. The crucial influential factors of annealing temperature were systematically explored on the catalytic activity and stability of IrO2/TiO2. Although increasing annealing temperature is believed to be of benefit to the increase of crystallinity and nanoparticle size in the catalyst, thus improving the stability of the catalyst, the as-prepared catalyst annealed at high temperature exhibited inferior stability at a constant-current density working state. This result is mainly attributed to the adverse impact of the annealing process at high temperatures on the catalytic activity, in terms of decreasing the active surface area due to the aggregation effect and decreasing the intrinsic activity due to the formation of a highly crystalline structure. The inferior catalytic activity caused by the adverse impact can induce a large working voltage, which can accelerate the catalyst degradation via a positive feedback mechanism (PFM) beneath the electrocatalytic system consisting of 'catalytic activity-electrocatalytic performance (working voltage)-aging'. Besides, the influences of Cl residue on the catalytic activity and stability of the as-prepared IrO2/TiO2 were also investigated by substituting a Cl-free precursor using IrCl3 (IrO2/TiO2-Cl). It presented slightly inferior initial oxygen evolution reaction (OER) electrocatalytic performance to that using a Cl-free precursor, perhaps due to the strong adsorption of Cl residue on IrO2 nanoparticles. However, the stability of the as-prepared IrO2/TiO2-Cl deteriorates significantly in chronopotentiometric measurement probably due to the PFM. Consequen

关键词： Annealing

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Establishment of a real-time fluorescence PCR method for the determination of snail meat components in Liuzhou snail rice noodles

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INTERnational JOURNAL OF FOOD SCIENCE AND TECHNOLOGY 2025年第1期60卷

作者： Lu, Siyuan Xing, Ranran Zhang, Jiukai Zhao, Mi Wei, Wei Chen, Ying Li, Qian Deng, Tingting Dalian Polytech Univ Sch Biol Engn Dalian Peoples R China Chinese Acad Inspect & Quarantine Beijing Peoples R China State Adm Market Regulat Key Lab Food Authent Identificat Beijing Peoples R China Liuzhou Qual Inspect & Testing Res Ctr Liuzhou Peoples R China

In recent years, because of a booming development of the snail rice noodles industry, investigations of Liuzhou Quality Inspection and Testing research Centre have revealed many snail rice noodle products in the market contain adulterated snail meat ingredients. Therefore, we developed a real-time fluorescence PCR assay for the rapid identification of snail meat components in snail rice noodles. The 16S ribosomal RNA genes of Cipangopaludina and Bellamya used as target genes for TaqMan real-time fluorescence PCR were designed. The assay for the detection of snail meat is highly specific, and the detection limit is 0.05% snail meat (w/w). When the assay was applied to commercially available snail rice noodle soups, the samples that contained snail meat tested positive, whereas none of the samples that did not contain snail meat yielded amplification curves. The assay allows rapid and efficient authenticity screening of snail meat components in rice noodles.

关键词： snail meat 16S rRNA gene real-time fluorescence PCR authenticity screening

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Tobacco-waste homologous red-/blue-emitting carbon dots for enhancing photosynthesis

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JOURNAL OF MATERIALS CHEMISTRY A 2025年第15期13卷 10801-10813页

作者： Wang, Hongyang Yang, Ning Zhao, Shijie Dong, Boru Buresova, Zuzana Bures, Filip Liu, Jialei Chinese Acad Agr Sci CAAS Inst Environm & Sustainable Dev Agr Minist Agr & Rural Key Lab Prevent & Control Residual Pollut Agr Film Beijing 100081 Peoples R China Shenyang Agr Univ Plant Protect Coll Shenyang 110866 Peoples R China Taiyuan Univ Technol Minist Educ Key Lab Interface Sci & Engn Adv Mat Taiyuan 030024 Shanxi Peoples R China Inst Technol & Business Ceske Budejovice Okruzni 517-10 Ceske Budejovice 37001 Czech Republic CAAS Zhongyuan Res Ctr Xinxiang 453003 Henan Peoples R China

Carbon dots (CDs), as a medium for enhancing photosynthesis, have attracted widespread attention, among which biomass-based CDs are ideal candidate materials owing to their multiple advantages. However, most of the biomass-based CDs are fabricated using a single synthesis strategy and employed directly, lacking rational classification and screening of diverse CD types within the products to identify homologous CDs that could maximize plant photosynthetic utilization efficiency. Hence, two types of CDs, blue (B-CDs) and red (R-CDs), were obtained from tobacco waste through a facile hydrothermal or solvothermal synthesis strategy. The prepared CDs proved to have tunable structures and properties, including tunable photoluminescence. The latter property matched well with the absorption spectra of chlorophyll and enabled the construction of chloroplast/CD hybrid photosynthesis systems. The 1 : 1 combination of B-CDs and R-CDs showed the most pronounced effect on the chloroplast photosynthetic activity, exhibiting a maximum increase of 367%. This significant improvement was attributed to CDs, which converted the energy of ineffective ultraviolet (B-CDs) and yellow-green light (R-CDs), thereby enhancing plant photosynthesis. Further in vivo studies showed that the 1 : 1 mixture of both CDs had a positive effect on light capture and absorption, acceleration of the electron transfer, CO2 assimilatory power, chlorophyll content, photosynthetic activity, psbA gene expression, and Rubisco activity, all of which promoted plant photosynthesis. Improved accumulation of lettuce biomass and nutritional quality were other advantageous effects. In summary, both the developed CDs proved to be very efficient and highly applicable CD-based photosynthetic fertilizers.

关键词： Tobacco

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Nitrogen isotopes of marine oils in the Tarim Basin,china:Implications for the origin of organic matters and the paleoenvironment

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Petroleum Science 2024年第6期21卷 3692-3705页

作者： Yang Bai Jian-Fa Chen Wen-Zhe Gang Xin-Jian Zhu Vaclav Suchy Shuai-Qi Tang Jin Wu Min Li Sheng-Bao Shi National Key Laboratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing102249China College of Geoscience China University of Petroleum(Beijing)Beijing102249China PetroChina Hangzhou Research Institute of Geology Hangzhou310023ZhejiangChina Nuclear Physics Institute v.v.i.Academy of Sciences of the Czech RepublicNa Truhlarce 39/6418086 Prague 8Czech Republic Chinese Academy of Geological Sciences Beijing100037China

Nitrogen isotope compositions(δ15N)of sedimentary rocks are usually used to reconstruct the paleoenvironment and nitrogen(N)biogeochemical ***δ15N values of crude oils inherit the characteristics of relevant source rocks and can well reflect the information of hydrocarbon-forming organisms and environment in ancient water ***,studies on theδ15N of crude oils are limited due to the low N *** this study,a new efficient method is applied to the marine oils from the Bashituo(BST)and Halahatang(HLHT)areas of the Tarim Basin to obtain the nitrogenous components(i.e.,nonhydrocarbons and asphaltenes)for the achievement of N *** carbon and nitrogen isotopes of these components and the biomarkers of oils were ***δ15N values in asphaltenes(δ15NAsp)are significantly heavier than those in nonhydrocarbons(δ15NNSOs)in these oils,which are attributed to the potential directional N transfer and kinetic isotope fractionation during the thermal evolution of organic matters(OM).Theδ15NAspvalues have significant correlations with OM origin associated parameters and weak correlations with environmental parameters,suggesting that the difference inδ15NAspvalues is mainly resulted from biological source rather than redox ***δ15NNSOsvalues have a closer relationship with the redox condition than biological characteristics,indicating that they have a good response to paleoenvironmental variation in the water column,which is not completely overprinted by the difference of OM *** redox conditions give rise to distinct nitrogen cycles,resulting in variousδ15N *** occurs in the water column of the Early Cambrian dominated by physically stratified conditions with significant isotope fractionation,resulting in relatively heavierδ15N of OM in the BST *** the Middle-Late Ordovician period,the limited suboxic zone leads to an insignificant positive bias ofδ15N caused by partial denitrification in the HLHT *** ev

关键词： Nitrogen isotope Nitrogenous components Crudeoils Organic matter Redox conditions Nitrogen cycle

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Dual Copper/Photoredox Catalysis for Radical-Mediated Arylation and Alkylation of Sulfenamides

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ACS CATALYSIS 2025年第5期15卷 4007-4016页

作者： Zhang, Mingjun Tan, Yuhao Yang, Hehe Fu, Xiaoyang Liu, Yuxiu Wang, Ziwen Wang, Qingmin Nankai Univ Res Inst Elemento Organ Chem Coll Chem Frontiers Sci Ctr New Organ MatterState Key Lab E Tianjin 300071 Peoples R China Tianjin Normal Univ Coll Chem Tianjin Key Lab Struct & Performance Funct Mol Tianjin 300071 Peoples R China

Radical-based pathways provide an attractive approach for constructing C(sp2/sp3)-S bonds from various substrates. Herein we report two strategies that can be used for aryl radical sulfilimination of aryl sulfonium salts and aryl radical-mediated cross-coupling reactions between alkyl iodides and sulfenamides, both via synergetic photoredox and copper catalysis. These mild, operationally simple reactions have a broad substrate scope and potential utility for late-stage functionalization of natural products and drug molecules. In addition, both sulfilimination reactions can be carried out on a gram scale under continuous-flow conditions. Mechanistic studies indicate that rapid abstraction of the iodine atom from the alkyl iodide by sterically hindered, electron-rich aryl radicals and tuning of the electronic properties of the copper catalyst by varying the ligand contribute to the chemoselectivity for alkylation.

关键词： sulfilimines aryl radical-mediated XAT arylsulfonium salts alkyl iodides photochemistry

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A review of the role of bioactive components in legumes in the prevention and treatment of cardiovascular diseases

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FOOD & FUNCTION 2025年第3期16卷 797-814页

作者： Chen, Mengqian Li, You Liu, Xinqi Beijing Technol & Business Univ China Food Flavor & Nutr Hlth Innovat Ctr Beijing 100048 Peoples R China Beijing Technol & Business Univ Natl Soybean Proc Ind Technol Innovat Ctr Beijing 100048 Peoples R China

Cardiovascular diseases (CVD) represent a primary global health challenge. Poor dietary choices and lifestyle factors significantly increase the risk of developing CVD. Legumes, recognized as functional foods, contain various bioactive components such as active peptides, protease inhibitors, saponins, isoflavones, lectins, phytates, and tannins. Studies have demonstrated that several of these compounds are associated with the prevention and treatment of cardiovascular diseases, notably active peptides, saponins, isoflavones, and tannins. This review aims to analyze and summarize the relationship between bioactive compounds in legumes and cardiovascular health. It elaborates on the mechanisms through which active ingredients in legumes interact with risk factors for cardiovascular diseases, such as hypertension, hypercholesterolemia, endothelial dysfunction, and atherosclerosis. These mechanisms include, but are not limited to, lowering blood pressure, regulating lipid levels, promoting anticoagulation, enhancing endothelial function, and modulating TLR4 and NF-kappa B signaling pathways. Together, these mechanisms emphasize the potential of legumes in improving cardiovascular health. Additionally, the limitations of bioactive components in legumes and their practical applications, with the goal of fostering further advancements in this area were discussed.

关键词： Tannins

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Physical Cross-Linking of Cellulose Nanofibrils with Zein Particles as an Eco-Friendly Detergent

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LANGMUIR 2025年第8期41卷 5147-5157页

作者： Liu, Wenli Wang, Wenxue Cui, Yanru Liu, Jiayu Liu, Pengtao Tianjin Univ Sci & Technol State Key Lab Biobased Fiber Mfg Technol Tianjin 300457 Peoples R China Tianjin Univ Sci & Technol Tianjin Key Lab Pulp & Paper Tianjin 300457 Peoples R China Tianjin Univ Sci & Technol China Light Ind Key Lab Papermaking & Biorefinery Tianjin 300457 Peoples R China

Pickering particles can self-assemble to form a rigid barrier film at the oil-water interface, effectively inhibiting droplet aggregation and stabilizing the emulsion. This essay explores the potential of Zein/TOCNF (TEMPO-oxidized cellulose nanofiber) complex aqueous dispersion as an environmentally friendly, nontoxic, versatile, and inexpensive cleaning agent based on the Pickering effect. The Zein/TOCNF complex exhibited an improvement in its surface hydrophobicity compared to that of pure TOCNF, so that it could be used as Pickering emulsion stabilizers. The Zein/TOCNF complex has better washing ability than the washing powder for the removal of the dirt from the cotton cloth, the glass, the stainless steel, the ceramic, and the plastic sheet. In addition, residual amounts of Zein/TOCNF complexes on the fabric were insignificant. Moreover, research results have shown that Zein/TOCNF complexes are feasible as a secure, cost-effective, and sustainable alternative to commercial washing cleaners.

关键词： Emulsification

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