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An in-situ tool wear measurement method based on super-pixels and enhanced corner detection algorithm

MEASUREMENT SCIENCE AND TECHNOLOGY 2025年第1期36卷 015001-015001页

作者： Wang, Zhizhuo Wang, Guofeng Wang, Haotian Li, Xuwei Yan, Shuang Sheng, Yanliang Tianjin Univ Sch Mech Engn Tianjin Peoples R China

In robotic milling scenarios, lighting conditions that are difficult to optimize and random angles of the cutting edge often lead to inaccuracies in in-situ tool wear detection. This paper proposes a method based on machine vision to accurately measure the wear parameters of end mills. Firstly, a cropping algorithm based on polar coordinate transformation is introduced to extract the Region of Interest from the flank face of the tool bottom. Subsequently, a segmentation algorithm that combines super-pixels and multi-thresholding is proposed, with a focus on maintaining the connectivity of the damage regions during segmentation. Finally, an algorithm based on improved corner detection is presented to reconstruct the tool breakage region. Experimental results demonstrate that the proposed method achieves precise tool wear measurement despite random cutting edge angles and exhibits good robustness under varying lighting conditions. In the in-situ detection experiment, the method achieved a measurement accuracy of 97% for maximum wear width and 95% for wear area, indicating the effectiveness of the proposed method.

关键词： machine vision tool wear super-pixels corner detection

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Identification of interferon-stimulated response elements (ISREs) in canines

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BMC VETERINARY research 2025年第1期21卷 1-14页

作者： Pan, Zhichao Liu, Yutong Dai, Huilin Xu, Siqi Qin, Miaomiao Wang, Ke Luo, Chenying Luo, Changqi Zhang, Qinying Liang, Zhaoping Feng, Saixiang South China Agr Univ Coll Vet Med Guangzhou Peoples R China Key Lab Zoonosis Prevent & Control Guangdong Prov Guangzhou Peoples R China South China Agr Univ Guangdong Lab Lingnan Modern Agr Guangzhou Peoples R China Minist Agr Key Lab Vet Vaccine Innovat Guangzhou Peoples R China Natl & Reg Joint Engn Lab Medicament Zoonosis Prev Guangzhou Peoples R China

Interferon (IFN) responses are vital for antiviral defense, with interferon-stimulated response elements (ISREs) crucial for regulating IFN signaling. While ISREs are well-studied in humans and mice, research on canine ISREs is limited. This study aimed to clarify the role of canine ISREs and create a new method for detecting IFN activity. Canine IFN alpha (CaIFN alpha) was produced using the Pichia pastoris (P. pastoris) system, and an ISRE-based flow cytometry method was developed to measure its activity. ISREs for CaIFN alpha were predicted via bioinformatics analysis. Subsequently, viral suppression assays were conducted using vesicular stomatitis virus, canine influenza virus, and H9N2 to evaluate the antiviral activity of recombinant CaIFN alpha. Fluorescence analysis confirmed that CaIFN alpha activates ISRE2, ISRE8, and ISRE10, thereby enhancing the transcription and expression of the enhanced green fluorescent protein (EGFP) fusion gene. A novel ISRE and EGFP based flow cytometry method enabled precise quantification of CaIFN alpha levels through fluorescence cell counts, with a detection sensitivity reaching 0.1 x 10- 7 mg/mL. Results demonstrate that CaIFN alpha possesses multiple antiviral activity and activates specific ISREs, augmenting gene expression. This approach advances the study of canine ISREs and supports the development and clinical application of CaIFN alpha for diagnosing viral infections and monitoring treatment efficacy.

关键词： Canine interferon alpha Fluorescent reporter assay ISRE Flow cytometry Antiviral activity EGFP

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Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta)

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MOLECULAR HORTICULTURE 2025年第1期5卷 1-19页

作者： Li, Yukuo Song, Zhe Zhan, Xu Li, Xiaohan Ye, Lingshuai Lin, Miaomiao Wang, Ran Huang, Hailei Guo, Jian Sun, Leiming Gu, Hong Chen, Jinyong Fang, Jinbao Qi, Xiujuan Chinese Acad Agr Sci Zhengzhou Fruit Res Inst Natl Key Lab Germplasm Innovat & Utilizat Hort Cro Zhengzhou 450009 Peoples R China Chinese Acad Agr Sci Zhongyuan Res Ctr Xinxiang 453500 Peoples R China Zhengzhou Univ Sch Agr Sci Zhengzhou 450001 Peoples R China Henan Univ Sci & Technol Coll Hort & Plant Protect Luoyang 471000 Peoples R China Shiyan Econ Crops Res Inst Shiyan Peoples R China Shandong Agr Univ Coll Hort Sci & Engn Tai An 271018 Peoples R China

Actinidia arguta is a newly emerged, commercially cultivated Actinidia species. A. arguta has a beautiful appearance and is rich in anthocyanin, and is thus highly welcomed by consumers. However, the mechanism of anthocyanin regulation in A. arguta remains unclear. In this study, we assembled the nearly complete genome of the first red A. arguta cultivar, 'Tianyuanhong', with an N50 of 21 Mb. Comparative genome analysis revealed a role of the expansion/contraction of gene families in the species-specific trait formation of A. arguta. Through verification of transient overexpression and stable transformation, RNA-seq analysis revealed a key bHLH transcription factor, AaBEE1, which negatively regulates anthocyanin biosynthesis. DAP-seq analysis combined with Y1H, EMSA, Chip-qPCR and LUC suggested that AaBEE1 binds to the G-box of the AaLDOX promoter and suppresses its expression. Overall, we assembled the genome of A. arguta and clarified its AaBEE1-AaLDOX module-mediated molecular mechanism of anthocyanin regulation.

关键词： Genome assembly Comparative genome Gene expression Anthocyanin biosynthesis Actinidia arguta

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Microfluidic-Based dsRNA Delivery Nanoplatform for Efficient Spodoptera exigua Control

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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024年第22期72卷 12508-12515页

作者： Xie, Jinshan Zhang, Jiaxin Yang, Jingyi Wu, Shuqin Teng, Xuanming Han, Hongyu Xu, Yufang Qian, Xuhong Zhu, Weiping Yang, Yangyang East China Univ Sci & Technol Sch Pharm Shanghai Key Lab Chem Biol Shanghai 200237 Peoples R China East China Univ Sci & Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China East China Univ Sci & Technol Engn Res Ctr Pharmaceut Proc Chem Sch Pharm Minist EducShanghai Key Lab Chem Biol Shanghai 200237 Peoples R China East China Univ Sci & Technol Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met Sch Pharm Shanghai 200237 Peoples R China

Nanotechnology-based RNA interference (RNAi) offers a promising approach to pest control. However, current methods for producing RNAi nanopesticides are mainly implemented in a batch-to-batch manner, lacking consistent quality control. Herein, we present a microfluidic-based nanoplatform for RNA nanopesticide preparation using lipid nanoparticles (LNPs) as nanocarriers, taking advantage of the enhanced mass transfer and continuous processing capabilities of microfluidic technology. The dsRNA@LNPs were rapidly formed within seconds, which showed uniform size distribution, improved leaf wettability, and excellent dispersion properties. The delivery efficiency of dsRNA@LNPs was evaluated by targeting the chitin synthetase B (CHSB) gene ofSpodoptera exigua. The dsRNA@LNPs can effectively resist nuclease-rich midgut fluid degradation. Importantly, dsCHSB@LNPs exhibited increased mortality rates, significant reduction of larvae growth, and enhanced gene suppression efficiency. Therefore, a continuous nanoplatform for RNAi nanopesticide preparation is demonstrated by utilizing microfluidic technology, representing a new route to produce RNAi nanopesticides with enhanced quality control and might accelerate their practical applications.

关键词： nanopesticide RNA interference microfluidictechnology lipid nanoparticle Spodopteraexigua

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Current-Tuned Tunable In-Series DFB Laser Array Assisted With Thin Film Heater

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IEEE PHOTONICS TECHNOLOGY LETTERS 2024年第15期36卷 921-924页

作者： Li, Zizhuo Sun, Zhenxing Zhao, Jie Xu, Zhenzhen Yang, Zijiang Wang, Zhuoying Zhang, Yue Yin, Zhiqian Nie, Jiaqiang Wang, Wenxuan Xiao, Rulei Chen, Xiangfei Nanjing Univ Key Lab Intelligent Opt Sensing & Manipulat Natl Lab Solid State Microstruct Coll Engn & Appl SciMinist Educ Nanjing 210093 Peoples R China Nanjing Univ Inst Opt Commun Engn Nanjing 210093 Peoples R China Jiangsu Univ Sci & Technol Ocean Coll Zhenjiang 212000 Peoples R China

In this letter, we propose and demonstrate a tunable in-series distributed feedback (DFB) laser array, and the wavelength is continuously tuned with the assistance of the monolithically integrated thin-film heater. Compared with an in-parallel laser array with an optical combiner, the proposed in-series DFB laser array avoids significant power loss resulting from the optical combiner. The three-section structure is utilized to suppress the grating crosstalk among the in-series laser array and guarantees single-mode stabilities. Besides, the reconstruction-equivalent-chirp technique is utilized to simplify grating fabrication and enhance the precise control of the grating phase. Four lasers with wavelength spacings of 2.4 nm are integrated into the in-series laser array and the Ti/Pt thin film heaters are monolithically integrated near the waveguides of each laser unit. The wavelength of the proposed structure is coarsely tuned by selecting the working laser and finely tuned by adjusting the injection current to the laser unit and the thin-film heaters. 9.6 nm continuous wavelength tuning range and fast wavelength tuning are obtained for the proposed in-series laser array without adjusting the temperature of the thermal-electric cooler (TEC). The proposed structure provides a solution for the wavelength tunable laser, which is promised to be used in the dense wavelength division multiplexing (DWDM) systems.

关键词： Distributed feedback laser array tunable laser thin film heater reconstruction-equivalent-chirp technique

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CMT-SRv6: A Customizable Multipath Transmission Scheme for 6G Edge Network

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IEEE SENSORS JOURNAL 2024年第24期24卷 42138-42151页

作者： Zhao, Ying Liao, Xingxing You, Wei Wang, Min Yang, Jie Ji, Xinsheng Li, Cong Southeast Univ Sch Cyber Sci Engn Nanjing 211102 Peoples R China Purple Mt Labs Nanjing 211111 Peoples R China Informat Engn Univ Zhengzhou 450001 Peoples R China Xidian Univ Sch Cyber Engn Xian 710126 Peoples R China

5G continuously advances deep integration with vertical industries, facilitated by technologies such as network slicing. As a further evolution, the architecture design of 6G will continue to deepen the user plane sinking, targeting more diversified edge scenarios. In this regard, the sinking user plane will expose the previously closed bearer network to untrusted domains, leading to more security challenges in data transmission. In addition, diverse edge scenarios often have differentiated transmission requirements, urgently requiring transmission strategies that can provide customizable performance metrics. Furthermore, the development trend of customizable services renders the traditional GPRS tunneling protocol (GTP) inadequate due to its lack of underlying network awareness, positioning segment routing over IPv6 (SRv6) as a viable alternative given its powerful programmability. Hence, this article proposes a customizable multipath transmission scheme (CMT-SRv6) based on the information dispersal algorithm (IDA) and multipath routing for SRv6 transmission. It supports dynamically adjusting transmission strategies for scenarios with different security levels and integrates with the 6G network architecture. The simulation section comprehensively considers security, reliability, and cost, verifying that the comprehensive performance of this solution is optimal in various security scenarios. A prototype system based on the proposed solution is built and validated in terms of feasibility, security, and reliability. Results show that CMT-SRv6 has better throughput and effectively enhances the security and reliability of network transmission.

关键词： Security Reliability 6G mobile communication Reliability theory Data communication Sensors Costs Encoding Industries 5G mobile communication 6G edge network information dispersal algorithm (IDA) multipath transmission SRv6

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Easy-to-perform organic-solvent-free synthesis of carbon dots with strong green photoluminescence

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Chinese Chemical Letters 2024年第3期35卷 373-378页

作者： Jiazhuang Guo Yifeng Chen Pan Zhang Ge Li Xiaoning Yang Cai-Feng Wang Su Chen State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing 210009China

Carbon dots(CDs)have been extensively studied owing to their fascinating optical properties and wide potential ***,we report an easy-to-perform and organic-solvent-free synthesis strategy for green-emissive CDs(G-CDs)possessing high photoluminescence(PL)quantum yield(QY).The G-CDs are synthesized by heating the homogeneous precursors of citric acid and cyanamide in an open vessel,circumventing the use of organic solvents,complex operations,high-pressure reactors,and expensive instruments in the synthesis *** effect of various reaction variables on the formation and the optical properties of G-CDs are systematically *** resultant G-CDs show bright PL emission at 521 nm with PL QY up to 73%.Then a white light-emitting diode(LED)with Commission Internationable de L'Eclairage(CIE)coordinates of(0.33,0.34)and color rendering index(CRI)of 92 is constructed based on G-CDs/thermoplastic polyurethane(TPU)***,a visual microfluidic detection platform is designed by using G-CDs as fluorescent probes for rapid quantitative detection of Fe^(3+),Cu^(2+),and Mn^(2+)metal ions,which can realize synchronized testing of multiple *** study might promote the development and preparation methods of high-performance CDs with various optical applications.

关键词： Carbon dots Efficient synthesis Green fluorescence Quantum yield White LEDs Microfluidic detection platform

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Enhancing rice (Oryza sativa L.) yield and quality by improving photosynthesis with foliar application of zinc oxide nanoparticles

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ENVIRONMENTAL SCIENCE-NANO 2025年第4期12卷 2331-2342页

作者： Zhang, Haipeng Chen, Jie Liu, Xinyue Wang, Rui Zhang, Hongcheng Yang, Yanju Yangzhou Univ Res Inst Rice Ind Engn Technol Coinnovat Ctr Modern Prod Technol Grain Crops Key Lab Cultivated Land Qual Monitoring & Evaluat Yangzhou 225009 Peoples R China

Zinc (Zn) is a critical co-factor for enzymes involved in photosynthesis, delaying leaf senescence and enhancing photosynthetic efficiency. Supplementing rice leaves with Zn can improve yield, quality, and Zn content in edible parts, addressing food security and micronutrient deficiencies. In this study, we evaluated the effects of spraying Zn oxide nanoparticles (ZnO NPs) (0, 5, 10, and 20 mg L-1) at the rice panicle initiation stage on photosynthesis, yield, and grain quality through a two-year field experiment. Results showed that foliar application of ZnO NPs at the panicle initiation stage increased the leaf area index, net photosynthetic rate, and photosynthetic potential, leading to a 1.5-6.4% increase in grain yield through a higher grain filling rate and 1000-grain weight. ZnO NPs also delayed leaf senescence and prolonged the duration of active photosynthesis, which significantly contributed to higher biomass production and improved grain filling, further enhancing yield. Additionally, the enhancement in photosynthetic efficiency and delayed senescence promoted the production of high-quality grains. ZnO NPs improved rice appearance quality by reducing the chalkiness grain rate and degree. The rice tasting value increased by 3.3-7.0%, reflecting improvements in appearance, viscosity, and balance, along with reductions in hardness. ZnO NPs raised peak viscosity and breakdown values while lowering setback values. Furthermore, ZnO NPs significantly increased Zn content in brown and milled rice by 13.8-56.0% and 20.1-78.6%, respectively, and improved Zn bioavailability by reducing the phytate-to-zinc molar ratio. These findings highlight the potential of ZnO NPs as a sustainable nanotechnology-based approach to simultaneously improve rice productivity, quality, and nutritional value, offering a promising solution for addressing food security and micronutrient deficiency in rice-based diets.

关键词： Food chemistry

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BRD4 promotes immune escape of glioma cells by upregulating PD-L1 expression

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JOURNAL OF NEURO-ONCOLOGY 2025年第3期171卷 669-679页

作者： Liu, Yongsheng Cai, Lize Wang, Hao Yao, Lin Wu, Yue Zhang, Kai Su, Zuopeng Zhou, Youxin Soochow Univ Affiliated Hosp 1 Neurosurg & Brain & Nerve Res Lab Suzhou Peoples R China

PurposeGlioblastoma multiforme (GBM) poses significant challenges in treatment due to its aggressive nature and immune escape mechanisms. Despite recent advances in immune checkpoint blockade therapies, GBM prognosis remains poor. The role of bromodomain and extraterminal domain (BET) protein BRD4 in GBM, especially its interaction with immune checkpoints, is not well understood. Our study aimed to explore the role of BRD4 in GBM, especially the immune *** this study, we performed bioinformatics gene expression and survival analysis of BRD4 using TCGA and CGGA databases. In addition, we investigated the effects of BRD4 on glioma cell proliferation, invasion and migration by clone formation assay, Transwell assay, CCK8 assay and wound healing assay. Chromatin immunoprecipitation (ChIP) assay was conducted to confirm BRD4 binding to the programmed death ligand 1 (PD-L1) promoter. GL261 cells with BRD4 shRNA and/or PD-L1 cDNA were intracranially injected into mice to investigate tumor growth and survival time. Tumor tissue characteristics were analyzed using H&E and IHC staining and immune cell infiltration were assessed by flow *** results showed that elevated expression of BRD4 in high-grade gliomas was associated with poor patient survival. In addition, we validated the promotional effects of BRD4 on glioma cell proliferation, invasion and migration. The results of ChIP experiments showed that BRD4 is a regulator of PD-L1 at the transcriptional level, implying that it is involved in the immune escape mechanism of glioma cells. In vivo studies showed that BRD4 knockdown inhibited tumor growth and reduced immunosuppression, improving ***4 has the capability to regulate the growth of glioblastoma and enhance immune suppression by promoting PD-L1 expression. Targeting BRD4 represents a promising direction for future research and treatment.

关键词： BRD4 GBM Immunosuppression PD-L1 Target therapy

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Genome-Wide Identification, Evolution, and Characterization of GATA Gene Family and GATA Gene Expression Analysis Post-MeJA Treatment in Platycodon grandiflorum

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JOURNAL OF PLANT GROWTH REGULATION 2025年第1期44卷 155-167页

作者： Ren, Weichao Kong, Lingyang Jiang, Shan Ma, Lengleng Wang, Honggang Li, Xiangquan Liu, Yunwei Ma, Wei Yan, Xueying Heilongjiang Univ Chinese Med Coll Pharmaceut Sci Harbin 150040 Heilongjiang Peoples R China Yichun Branch Heilongjiang Acad Forestry Yichun 153000 Peoples R China

The GATA-binding factor (GATA) plays a major role in regulating plant development and response to distinct environmental stresses. At present, GATAs are characterized in various model plant species, including Arabidopsis thaliana and Oryza sativa. However, the GATA gene family in Platycodon grandiflorum is not yet fully understood. The study aimed to develop a comprehensive understanding of the GATA TFs and explore the regulatory mechanism of methyl jasmonate (MeJA) on the GATA members in P. grandiflorum. A total of 22 PgGATAs were identified based on publicly available genome data of P. grandiflorum, and each member was analyzed in detail. The 22 identified genes were distributed across nine chromosomes. Their phylogenetic tree and domain structures showed that the GATAs could be clustered into four subfamilies (A-D). The structural protein domains and conserved motifs of the PgGATA family members were relatively conserved across different subfamilies. Light and hormone response elements were found in abundance in the promoter sequences. In addition, quantitative real-time polymerase chain reaction (qRT-PCR) indicated that PgGATA4, 6, 7, 8, and 11 were sensitive to MeJA treatment in P. grandiflorum roots. Nevertheless, co-expression network analysis revealed that the activities of the genes in the family remained significantly correlated, suggesting possible synergy in their functions. Two (PgGATA5 and PgGATA9) and three (PgGATA8, PgGATA11, and PgGATA22) hub PgGATAs were identified that might have central functions in P. grandiflorum tissues and MeJA-treated roots, respectively. This study provided detailed information about the PgGATA gene family and facilitated a functional characterization of the candidate genes.

关键词： Platycodon grandiflorum Genome-wide GATA transcription factors Gene expression MeJA

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