Reverse transcription-polymerase chain reaction(RT-PCR)was used to amplify the Small Envelope(E)gene of avian Infectious bronchitis virus(IBV)LX4 strain and the gene was cloned into the pMD18-T *** digestion with rest...
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Reverse transcription-polymerase chain reaction(RT-PCR)was used to amplify the Small Envelope(E)gene of avian Infectious bronchitis virus(IBV)LX4 strain and the gene was cloned into the pMD18-T *** digestion with restriction enzymes SalⅠand BamHⅠ,the E gene was subcloned into pET-30a vector to construct recombinant plasmid *** recombinant plasmid was transformed into *** BL21(DE3)and induced with *** was demonstrated by SDS-PAGE that a protein of 14kDa,which was comparable in size to the native E protein,was expressed in *** 14KDa protein was purified and used to prepare the rabbit antiserum against IBV E *** result showed that the antibody could react with purified E protein in Western blotting.
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