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中华传染病杂志 2024年第2期42卷 84-90页

作者：许文李阿敏李璟成艳张野空军军医大学第二附属医院(第四军医大学唐都医院)疾病预防控制科西安710038 空军军医大学第二附属医院(第四军医大学唐都医院)传染病科西安710038

目的探讨非围产期成人单核细胞性李斯特菌(LM)感染者的临床特征。方法纳入2010年1月至2023年7月第四军医大学唐都医院收治的经病原学确诊的25例非围产期李斯特菌病患者,回顾性分析患者人口学资料、基础疾病、临床表现、实验室检查指标... 详细信息

目的探讨非围产期成人单核细胞性李斯特菌(LM)感染者的临床特征。方法纳入2010年1月至2023年7月第四军医大学唐都医院收治的经病原学确诊的25例非围产期李斯特菌病患者,回顾性分析患者人口学资料、基础疾病、临床表现、实验室检查指标、头颅影像学表现、抗感染治疗方案、临床转归等特征。分别比较李斯特菌败血症组和李斯特菌性脑膜脑炎组、康复组和死亡组患者的临床特征。统计学分析采用独立样本t检验和曼-惠特尼U检验。结果25例患者年龄为(52.84±12.17)岁,男18例,女7例,主要临床表现为发热[23/25(92%)]、头痛[15/25(60%)]、意识障碍[12/25(48%)]、呕吐[8/25(32%)],13例(52%)患者存在基础疾病(包括血液系统疾病、自身免疫病、实体肿瘤、肝硬化等)。李斯特菌败血症组和李斯特菌性脑膜脑炎组在血常规、转氨酶、蛋白质水平、肾功能、电解质、血糖、炎症指标之间的差异均无统计学意义(均P>0.05)。死亡组血小板计数高于康复组,差异有统计学意义[(243.10±92.96)×10^(9)/L比(157.80±75.55)×10^(9)/L,t=2.45,P=0.022],但康复组和死亡组在生物化学、炎症指标之间的差异均无统计学意义(均P>0.05)。李斯特菌性脑膜脑炎患者头颅影像学检查表现为颅内感染、脑水肿/脑积水或脑疝。19例李斯特菌性脑膜脑炎患者的脑脊液压力[220.0(130.0,290.0)mmH_(2)O(1 mmH_(2)O=0.0098 kPa)]、脑脊液细胞总数[522.0(350.0,783.0)×10^(6)/L]、脑脊液白细胞数[356.0(266.0,668.0)×10^(6)/L]和脑脊液蛋白质定量[1817.0(822.5,6000.0)mg/L]均升高,15例患者脑脊液氯化物降低[(111.70±8.51)mmol/L],10例患者脑脊液葡萄糖水平降低[3.71(1.45,7.11)mmol/L]。血培养和脑脊液培养药物敏感试验结果示LM对氨苄西林均敏感,分别有1例、3例、3例患者对青霉素、美罗培南、复方磺胺甲噁唑耐药。17例康复患者中13例使用基于氨苄西林或青霉素等敏感抗菌药物的治疗方案。结论罹患基础疾病、免疫功能低下的患者出现高热、中枢神经系统感染等临床症状时,应警惕李斯特菌病可能,早期应用以氨苄西林为基础的治疗方案有利于患者临床康复。

关键词：单核细胞增生利斯特菌中枢神经系统感染败血症临床特征

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单核细胞增生李斯特菌脑膜炎两例报道并文献复习

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实用心脑肺血管病杂志 2019年第11期27卷 114-117页

作者：魏冕程丽妞张亚恒河南科技大学临床医学院河南科技大学第一附属医院

单核细胞增生李斯特菌脑膜炎是由单核细胞增生李斯特菌(LM)感染引起的一种细菌性脑膜炎症,具有低发病率、高致死率的特点,常发生于免疫功能缺陷患者。LM脑膜炎临床表现缺乏特异性,易误诊为结核性脑膜炎而延误治疗。本文报道了两例LM脑... 详细信息

单核细胞增生李斯特菌脑膜炎是由单核细胞增生李斯特菌(LM)感染引起的一种细菌性脑膜炎症,具有低发病率、高致死率的特点,常发生于免疫功能缺陷患者。LM脑膜炎临床表现缺乏特异性,易误诊为结核性脑膜炎而延误治疗。本文报道了两例LM脑膜炎患者,其中第1例患者确诊为LM脑膜炎合并结核性脑膜炎,可能为首例LM脑膜炎合并结核性脑膜炎患者;并通过文献复习总结了LM脑膜炎的临床特点,以期为提高临床对LM脑膜炎的认识提供参考。

关键词：脑膜炎,利斯特菌性单核细胞增生利斯特菌结核,脑膜病例报告历史文献

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Listeria monocytogenes: a bacterial pathogen to hit on the SUMO pathway

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细胞研究（英文版） 2010年第7期20卷 738-740页

作者： Simona Citro Susanna Chiocca European Institute of Oncology Department of Experimental Oncology IFOM-IEO Campus Via Adamello 16 20139 Milan Italy

Attachment of ubiquitin (Ub) or ubiquitin-like (Ubl) modifiers, such as SUMO, is a reversible post-translational modification that regulates the fate and function of proteins.

关键词：蛋白质类泛素单核细胞增生利斯特菌胰岛素

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Evidence for the involvement of ActA in maturation of the Listeria monocytogenes phagosome

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细胞研究（英文版） 2010年第1期20卷 109-112页

作者： Mathilde A Poussin Howard Goldfine Department of Microbiology University of Pennsylvania School of Medicine Philadelphia PA 19104-6076 USA Ovarian Cancer Research Center-421 Curie Boulevard Room 1326-1328A BRB Ⅱ/Ⅲ University of Pennsylvania School of Medicine Philadelphia PA 19104 Department of Microbiology University of Pennsylvania School of Medicine Philadelphia PA 19104-6076 USA

Dear Editor,Listeria monocytogenes (Lm), a Gram-positive facultative bacterium, is the causative agent of listeriosis, a food-borne disease affecting humans [1]. During infections, Lm enters phagosomes from which it escapes into the cytoplasm. Listeriolysin O (LLO) is essential, and two phospholipases play a role in this process [1]. Actin polymerization provides the propulsive force that moves the bacteria through the cytoplasm and into adjacent cells. It is initiated by the interaction of the N-terminal domain of ActA, a 639 amino acid membrane protein, with the host Arp2/3 complex.

关键词：肌动蛋白类单核细胞增生利斯特菌磷脂酶类食品吞噬体细胞质细菌胰岛素人类

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基因芯片技术检测3种肠道病原微生物方法的建立

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生物技术通讯 2007年第3期18卷 441-445页

作者：李禾徐琦吴忠华金大智杨宁敏高爽浙江省国际旅行卫生保健中心浙江杭州310003 军事医学科学院放射与辐射医学研究所北京100850 杭州致远医学检验所浙江杭州310009

目的:建立一种运用多重PCR和基因芯片技术检测和鉴定伤寒沙门氏菌、痢疾杆菌和单核细胞增生利斯特菌的方法。方法:分别选取伤寒沙门氏菌染色体ViaB区域中编码调控Vi抗原表达的基因(vipR)、痢疾杆菌编码侵袭质粒抗原H基因(ipaH)和单核细... 详细信息

目的:建立一种运用多重PCR和基因芯片技术检测和鉴定伤寒沙门氏菌、痢疾杆菌和单核细胞增生利斯特菌的方法。方法:分别选取伤寒沙门氏菌染色体ViaB区域中编码调控Vi抗原表达的基因(vipR)、痢疾杆菌编码侵袭质粒抗原H基因(ipaH)和单核细胞增生利斯特菌溶血素基因(hlyA)设计引物和探针,探针3'端进行氨基修饰,下游引物标记荧光素Cy3。在优化的PCR和杂交反应条件下,进行三重PCR扩增,产物与包括3种致病菌特异性探针的基因芯片杂交。在评价基因芯片的特异性和灵敏度之后,对临床样本进行检测。结果:只有3种目的致病菌的PCR产物在相应探针位置出现特异性信号,其他阴性细菌均无信号出现;3种致病菌的检测灵敏度均可达到103CFU/mL;检测30例临床样本的结果与常规细菌学培养结果一致。结论:所建立的可同时检测伤寒沙门氏菌、痢疾杆菌和单核细胞增生利斯特菌的基因芯片方法快速、准确,特异性高,重复性好,为3种肠道致病菌的快速检测和鉴定提供了新方法和新思路。

关键词：基因芯片多重PCR 伤寒沙门氏菌痢疾杆菌单核细胞增生利斯特菌

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Selective amplification of bacterial RNA:: use of a DNA primer containing mismatched bases near its 3′ terminus to reduce false-positive signals

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LETTERS IN APPLIED MICROBIOLOGY 2000年第3期31卷 187-192页

作者： Koo, K Jaykus, LA N Carolina State Univ Dept Food Sci Coll Agr & Life Sci Raleigh NC 27695 USA N Carolina State Univ SE Dairy Foods Res Ctr Raleigh NC 27695 USA

A reverse transcription PCR (RT-PCR) method designed to reduce false-positive results due to the co-amplification of contaminating genomic DNA is reported. Feasibility of the method was evaluated using 16S rRnrA sequences specific to Bacillus cereus. A DNA oligonucleotide primer, consisting of 22-bases containing three consecutive mismatched bases near its 3' terminus (primer B16RT), was used for reverse transcription and in subsequent cDNA amplification. Specific rRNA was reverse transcribed at low temperature (40 degrees C or 45 degrees C) in the presence of primer B16RT. As subsequent PCR using primer B16RT at high (62 degrees C) annealing temperatures is not nearly as efficient as amplification using the specific primer, amplification of genomic DNA was hindered relative to the amplification of cDNA. The method was readily adapted to the selective amplification of mRNA of the Listeria monocytogenes listeriolysin O (hly) gene.

关键词： 3'非翻译区蜡状芽孢杆菌/遗传学蜡状芽孢杆菌/生长和发育细菌毒素类碱基错配/遗传学 DNA引物/遗传学假阳性反应热休克蛋白质类/遗传学溶血素蛋白质类单核细胞增生利斯特菌核酸扩增技术/方法 RNA 细菌/遗传学 RNA 细菌/分离和提纯 RNA 信使/遗传学 RNA 核糖体 16S/遗传学逆转录聚合酶链反应

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Survival of Listeria innocua in salmon following cold-smoke application

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JOURNAL OF FOOD PROTECTION 2000年第6期63卷 715-720页

作者： Sabanadesan, S Lammerding, AM Griffiths, MW Univ Guelph Dept Food Sci Guelph ON N1G 2W1 Canada Canadian Food Inspect Agcy Fish Seafood & Prod Div Nepean ON K1A 0Y9 Canada Hlth Canada Guelph ON N1G 3W4 Canada

The ability of Listeria innocua to survive on salmon fillets during cold smoking in a commercial processing plant was investigated using a central composite rotatable response surface design to examine smoking temperatures in the range of 18 to 30 degrees C and a smoke time from 2 to 14 h. Smoke temperature did not significantly (P < 0.05) reduce counts of L. innocua on the salmon. However, the smoking time had a significant effect on L. innocua. The smoking time was directly related to the reduction in count (R-2 = 0.831), and a 3-log cycle reduction in count was observed when the smoking time was 12 h. The reduction in L. innocua levels on the fish was unaffected by the pH, water activity, and salt concentration of the fillet.

关键词：鱼制品/微生物学食品保存/方法氢离子浓度利斯特菌属/分离和提纯单核细胞增生利斯特菌大麻哈鱼/微生物学烟氯化钠温度时间因素动物

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Inactivation of Listeria monocytogenes Scott A on artificially contaminated frankfurters by high-pressure processing

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JOURNAL OF FOOD PROTECTION 2000年第5期63卷 662-664页

作者： Lucore, LA Shellhammer, TH Yousef, AE Ohio State Univ Dept Food Sci & Technol Columbus OH 43210 USA

Vacuum-packaged frankfurters, inoculated with 24-h cultures of Listeria monocytogenes Scott A (similar to 10(9) CFU/ml) by injection into the packages, were held at pressures of 300, 500, and 700 MPa for up to 9 min. L. monocytogenes were washed from the surface of the frankfurter and plated onto brain heart infusion agar. During the time to achieve 300, 500, and 700 MPa (come-up time), L. monocytogenes populations decreased by 1, >3, and >5 logs, respectively. Additional inactivation of L. monocytogenes occurred while the samples were held at 300 and 500 MPa. A 5-log reduction in bacterial population was possible at all pressure treatments;however, pressurization at 700 MPa showed the fastest inactivation with L. monocytogenes reduced from 10(8) to 10(2) CFU/package during the come-up time. These results show that high-pressure processing may be a viable method for controlling foodborne pathogens in postprocessed, packaged frankfurters.

关键词：食品处理和加工/方法食品微生物学单核细胞增生利斯特菌肉制品/微生物学压力猪动物牛

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A predictive model to determine the effects of pH, milkfat, and temperature on thermal inactivation of Listeria monocytogenes

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JOURNAL OF FOOD PROTECTION 1999年第10期62卷 1143-1149页

作者： Chhabra, AT Carter, WH Linton, RH Cousin, MA Purdue Univ Dept Food Sci W Lafayette IN 47907 USA Virginia Commonwealth Univ Dept Biostat Richmond VA 23298 USA

Listeria monocytogenes is a foodborne pathogen of significance because of its comparatively high heat resistance, zero tolerance in ready-to-eat foods, and growth at refrigeration temperatures. A 3 x 3 x 3 factorial study was done to determine the effects of milkfat (0%, 2.5%, 5.0%), pH (5.0, 6.0, 7.0), and processing temperature (55 degrees C, 60 degrees C, 65 degrees C) on the thermal resistance of L. monocytogenes in a formulated and homogenized milk system. Data were fit to a modified Gompertz equation where parameter estimates characterized three regions of a survival curve: the shoulder, maximum slope, and tail. Statistical analysis was done for each of the 27 individual treatment sets to visualize individual effects on parameter estimates and to evaluate how well the Gompertz equation represented the data. A regression model for the Gompertz equation was generated to predict the logarithmic surviving fraction of L. monocytogenes based on all 27 treatments and their single and interactive effects. The shoulder region of the survival curve was affected by pH;however, the maximum slope was affected by temperature, milkfat, and the interaction of temperature x milkfat. Validation of the model suggests that the predictions are best suited for processing above 62 degrees C. Trends over time for a 4-log reduction in cells (4D values) were evaluated using results from the 27 individual treatment sets, the regression model for the Gompertz equation, and a linear equation. At lower temperatures, 4D values by the three methods varied by twofold. At higher temperatures, all methods gave similar 4D values, suggesting that death became more linear. Based on this study all three factors affect heat resistance for specific regions of a survival curve, and a predictive model was developed that can be used as a preliminary estimate for L. monocytogenes inactivation.

关键词：食品微生物学食品配方/微生物学氢离子浓度脂类/药理学单核细胞增生利斯特菌乳/微生物学温度动物

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Foodborne infections during pregnancy

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JOURNAL OF FOOD PROTECTION 1999年第7期62卷 818-829页

作者： Smith, JL USDA ARS Microbial Food Safety Res Unit Eastern Reg Res Ctr Wyndmoor PA 19038 USA

The consequences of foodborne illness can be particularly devastating during pregnancy because, both the woman and her fetus are at risk. Escalated production of progesterone during pregnancy leads to down-regulation of cellular (cell-mediated) immune functions. Many foodborne pathogens (and other pathogens) are intracellular pathogens, and infections caused by these pathogens are controlled by cell-mediated immunity. The pregnancy-induced decrease in cell-mediated immune functions leads to increased susceptibility of the pregnant woman to certain infections. Hepatitis E virus, Coxiella burnetii, Listeria monocytogenes, and Toxoplasma gondii are intracellular pathogens that have a predilection for the maternal-fetal unit and may induce serious disease:in the mother and/or fetus. In the United States, T. gondii and L. monocytogenes are the most important foodborne pathogens in pregnancy, and these organisms can induce death or grave disease in the fetus and newborn. The pregnant woman, in order to protect herself and her fetus from the consequences of foodborne illness, must practice a high standard of food hygiene and personal cleanliness.

关键词：食品微生物学食物传播疾病/并发症单核细胞增生利斯特菌李斯特菌病/并发症李斯特菌病/流行病学母体胎儿间交换妊娠并发症感染性弓形虫病/流行病学美国/流行病学女(雌)性人类妊娠

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