检索结果-内蒙古大学图书馆

Stimulation of human neutrophils by chemotactic factors is associated with the activation of phosphatidylinositol 3-kinase γ

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 2000年第31期275卷 23636-23641页

作者： Naccache, PH Levasseur, S Lachance, G Chakravarti, S Bourgoin, SG McColl, SR Univ Laval Ctr Rech Rhumatol & Immunol Quebec City PQ G1V 4G2 Canada Univ Laval Dept Physiol & Med Quebec City PQ G1V 4G2 Canada Univ Adelaide Dept Microbiol & Immunol Adelaide SA 5005 Australia

The activation of human polymorphonuclear neutrophil leukocytes (neutrophils) is associated with an increased synthesis of the highly phosphorylated phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P-3). The aims of the present investigation were to determine whether the newly described, G protein-dependent phosphatidylinositol 3-kinase (PI3K), p110 gamma, was involved in the responses to chemotactic factors interacting with G protein-coupled receptors. The presence of p110 gamma in neutrophils was first established both at the protein and the mRNA level. Stimulation of the cells with fMet-Leu-Phe or interleukin-8 increased the PI3K activity in p110 gamma, but not p85, immunoprecipitates. The time course of this effect (threshold within less than 5 s, maximal activation at 10-15 s) was consistent with that of the generation of PtdIns(3,4,5)P-3. Wortmannin, a PI3K inhibitor, abrogated the effects of Met-Leu-Phe, which were also significantly inhibited by pertussis toxin. Finally, fMet-Leu-Phe also induced a significant translocation of p110 gamma to a particulate fraction derived from these cells. These data indicate that p110 gamma represent the major PI3K activated by fMet-Leu-Phe and interleukin-8 at very early time points following the stimulation of human neutrophils.

关键词：雄二烯类/药理学生物转运细胞区室化趋化因子类/药理学白细胞介素8/药理学同工酶类/代谢 N-甲酰甲硫氨酰亮氨酰-苯丙氨酸/药理学嗜中性白细胞激活/生理学百白咳毒素 RNA 信使/分析毒力因子鲍特菌属/药理学成年人人类

来源：评论

学校读者我要写书评

暂无评论

Neutrophil production and function in newborn infants

引用

BRITISH JOURNAL OF HAEMATOLOGY 2000年第1期110卷 18-28页

作者： Carr, R Kings Coll London St Thomas Hosp Dept Haematol London SE1 7EH England

Presents a study which investigated neutrophil production and function in newborn infants. Morbidity rates among preterm neonates from bacterial infection; Kinetics of neutrophil production in immature newborn infants... 详细信息

关键词：细菌感染/免疫学血液杀菌作用趋化性白细胞粒细胞集落刺激因子/投药和剂量粒细胞巨噬细胞集落刺激因子/投药和剂量免疫球蛋白类静脉内婴儿新生/免疫学婴儿早产/免疫学白细胞计数白细胞生成嗜中性白细胞激活/生理学中性白细胞/生理学吞噬作用呼吸爆发动物人类大鼠

来源：评论

学校读者我要写书评

暂无评论

P-selectin inhibition prevents early neutrophil activation but provides only modest protection against myocardial injury in dogs with ischemia and forty-eight hours reperfusion

引用

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY 1999年第1期34卷 280-288页

作者： Arai, M Masui, Y Goldschmidt-Clermont, P DiPaula, A Siu, C Kondo, T Becker, LC Johns Hopkins Med Inst Dept Med Div Cardiol Baltimore MD 21287 USA

OBJECTIVES This study was designed to determine whether antibody neutralization of the adhesion protein P-selectin would prevent neutrophil activation and reduce myocardial reperfusion injury. BACKGROUND Although inhibition of P-selectin markedly reduces short-term myocardial injury after ischemia and reperfusion, it is unknown whether it can provide meaningful long-term protection and preserve left ventricular function. METHODS Closed-chest dogs underwent 90 min left anterior descending coronary artery occlusion and 48 h reperfusion, and were randomized to 1) a treatment group (n = 11) receiving 1 mg/kg of the blocking anti-P-selectin antibody PB1.3, or 2) a control group receiving 1 mg/kg PNB1.6 (nonblocking antibody against P-selectin, n = 7) or an equivalent volume of saline (n = 2) 10 min before reperfusion. Infarct size was assessed postmortem by triphenyl tetrazolium chloride staining. Contrast left ventriculography was used to measure left ventricular function. Activation of circulating polymorphonuclear neutrophils (PMNs) was assessed by an increase in surface CD18 expression. RESULTS Neutrophil activation was observed at 30 min after reperfusion in the control group, but was abolished in the treatment group. Infarct size was reduced about 25%, in the treatment group after controlling for variations in ischemic blood flow (p = 0.003, by analysis of covariance). However, this protective effect was not associated with preservation of blood flow to the ischemic-reperfused myocardium, nor with any improvement in global or regional left ventricular function. CONCLUSIONS The anti-P-selectin antibody PB1.3 prevented early PMN activation, but had only a modest long-term infarct-limiting effect over 48 h reperfusion. Adhesion molecules other than P-selectin may mediate delayed PMN activation and accumulation in reperfused myocardium. (C) 1999 by the American College of Cardiology.

关键词：抗体单克隆侧支循环冠状血管/生理学交叉反应疾病模型动物评价研究(主题) 心肌再灌注损伤/病理生理学心肌再灌注损伤/预防和控制嗜中性白细胞激活/生理学 P选择素/免疫学 P选择素/生理学随机分配局部血流动物狗女(雌)性

来源：评论

学校读者我要写书评

暂无评论

Regulation of p21rac activation in human neutrophils

引用

BLOOD 1999年第3期94卷 1121-1130页

作者： Geijsen, N van Delft, S Raaijmakers, JAM Lammers, JWJ Collard, JG Koenderman, L Coffer, PJ Univ Utrecht Hosp Dept Pulm Dis NL-3584 CX Utrecht Netherlands Netherlands Canc Inst Div Cell Biol Amsterdam Netherlands

The small guanosine triphosphate (GTPase) p21rac is highly expressed in human neutrophils where it is thought to play a role in cytoskeletal reorganization and superoxide production. Using the p21rac binding domain of PAK (PAK-RBD) as an activation-specific probe, we have investigated agonist-stimulated activation of p21rac. Stimulation of neutrophils with the chemoattractants fMet-Leu-Phe (fMLP) or platelet-activating factor (PAF) induced an extremely rapid and transient p21rac activation, being optimal within 5 seconds. This activation correlates with the rapid changes of intracellular free Ca2+ ([Ca2+](i)) stimulated by fMLP;however, changes in [Ca2+](i) were neither sufficient nor required for p21rac activation. Furthermore, fMLP-induced p21rac activation was not inhibited by broad tyrosine kinase inhibitors or specific inhibitors of ERK, p38 mitogen activated protein kinase, Src, or phosphatidylinositol 3-kinases. Surprisingly, the cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor-alpha did not cause p21rac activation or modulate fMLP-induced p21rac activation. AlF-, a potent activator of heterotrimeric G-protein cu-subunits, however, was found to activate p21rac. Stimulation of neutrophils with phorbol myristate acetate (PMA) strongly activated the respiratory burst, but did not induce p21rac activation, suggesting that superoxide production per se can occur independently of p21rac activation. These data suggest that in human granulocytes, G-protein coupled receptors, but not cytokine receptors, activate p21rac via a rapid, novel exchange-mechanism independently of changes in [Ca2+](i), tyrosine phosphorylation, or PI3K. (C) 1999 by The American Society of Hematology.

关键词：钙/生理学趋化因子类/药理学 GTP磷酸水解酶类/生理学 GTP结合蛋白质类/生理学粒细胞巨噬细胞集落刺激因子/药理学鸟苷三磷酸/生理学嗜中性白细胞激活/药物作用嗜中性白细胞激活/生理学中性白细胞/生理学血小板活化因子/药理学肿瘤坏死因子α/药理学 racGTP结合蛋白质类人类

来源：评论

学校读者我要写书评

暂无评论

Clinical evaluation of leukocyte-depleted blood cardioplegia for pediatric open heart operation

引用

ANNALS OF THORACIC SURGERY 2000年第6期69卷 1914-1919页

作者： Hayashi, Y Sawa, Y Nishimura, M Ichikawa, H Kagisaki, K Ohtake, S Matsuda, H Osaka Univ Grad Sch Med Dept Surg Course Intervent Med E1 Suita Osaka 5650871 Japan

Background. Blood cardioplegia (BCP) is widely used for myocardial protection during open heart operation. However, BCP may have a chance to induce neutrophil-mediated myocardial injury during aortic crossclamping. We clinically evaluated the myocardial protective effect of leukocyte-depleted blood cardioplegia (LDBCP) for initial and intermittent BCP administration in pediatric patients. Methods. Fifty patients undergoing open heart operation for congenital heart disease between January 1997 and March 1999 were reviewed. Twenty-five were administered LDBCP for myocardial protection during ischemic periods (LDBCP group), and the remaining 25 were given BCP without leukocyte depletion (BCP group). Results. The difference in plasma concentrations of malondialdehyde between coronary sinus effluent blood and arterial blood just after reperfusion in the LDBCP group (1.68 +/- 0.56 mu mol/L) was significantly lower than that in the BCP group (2.35 +/- 0.62 mu mol/L;p < 0.01). The LDBCP group showed significantly lower plasma concentrations of human heart fatty acid-binding protein at 50 minutes after reperfusion (LDBCP group, 103.5 +/- 38.7 IU/L;BCP group, 144.8 +/- 48.8 IU/L;p < 0.01) and the peak value of creatine kinase-MB during the first 24 postoperative hours (LDBCP group, 17.0 +/- 8.5 IU/L;BCP group, 26.0 +/- 11.6 IU/L;p < 0.01) than did the BCP group. The maximum dose of catecholamine was significantly smaller in the LDBCP group (LDBCP group, 3.20 +/- 2.18 mu g . kg(-1) . min(-1);BCP group, 5.60 +/- 2.83 mu g . kg(-1.) min(-1);p < 0.01). Conclusions. These results suggest the usefulness of LDBCP for protection from the myocardial injury that can be induced by BCP administration during aortic cross-clamping. (C) 2000 by The Society of Thoracic Surgeons.

关键词：输血自体心麻痹液心脏停搏人工心脏缺损先天性/血液心脏缺损先天性/外科学淋巴细胞去除术丙二醛/血液心肌再灌注损伤/血液嗜中性白细胞激活/生理学儿童儿童学龄前女(雌)性人类婴儿男(雄)性

来源：评论

学校读者我要写书评

暂无评论

Reperfusion injury: Experimental evidence and clinical implications

引用

AMERICAN HEART JOURNAL 1999年第2期138卷 S69-S75页

作者： Ambrosio, G Tritto, I Univ Perugia Div Cardiol Sch Med I-06122 Perugia Italy Azienda Ospedaliera Perugia Dipartimento Malattie Cardiovascolari Perugia Italy

Postischemic reperfusion may profoundly alter cardiac function. Principal mediators of this phenomenon are oxygen radicals and neutrophils, Upon reflow, oxygen radicals are generated in large amounts, overwhelming cellular defenses and inducing oxidative tissue damage;biochemical markers of oxygen radical formation and attack can be found in postischemic myocardium, Reintroduction of neutrophils in postischemic tissues is accompanied by their activation, with release of lytic enzymes that directly induce tissue damage and proinflammatory mediators that amplify the local inflammatory reaction. Neutrophils may also plug capillaries, mechanically blocking flow. Oxidants can also modulate various events, ultimately leading to tissue injury, such as nitric oxide formation, platelet-activating Factor metabolism, tissue factor synthesis, and exposure of adhesion molecules. In the clinical setting, important consequences of postischemic reperfusion are reversible contractile dysfunction ("stunning"), which is mostly caused by oxygen radical attack, and impairment to flow at the microvascular level ("no-reflow") secondary to neutrophil plugging and vasoconstriction.

关键词：毛细血管/病理学毛细血管/病理生理学细胞黏附分子/生理学冠状动脉循环/生理学冠状血管/病理学冠状血管/病理生理学自由基心脏/病理生理学炎症介导素类/生理学心肌缺血/病理生理学心肌缺血/治疗心肌再灌注损伤/病因学心肌再灌注损伤/病理生理学心肌顿抑/病理生理学心肌/代谢心肌/病理学嗜中性白细胞激活/生理学中性白细胞/生理学一氧化氮/代谢氧化性应激/生理学血小板活化因子/代谢活性氧凝血致活酶/生物合成人类

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：