检索结果-内蒙古大学图书馆

中华眼底病杂志 2010年第2期26卷 143-146页

作者：陈瑛马进朱铁培浙江大学医学院附属二院眼科中心杭州310009

目的观察发生糖尿病与未发病非肥胖性糖尿病（NOD）小鼠玻璃体内opticin蛋白表达差异.方法建立糖尿病NOD小鼠模型作为实验组.选用同龄NOD小鼠作为对照.颈椎脱臼法处死两组所有小鼠.摘除眼球,钝性分离玻璃体、视网膜、巩膜.采用蛋白质... 详细信息

目的观察发生糖尿病与未发病非肥胖性糖尿病（NOD）小鼠玻璃体内opticin蛋白表达差异.方法建立糖尿病NOD小鼠模型作为实验组.选用同龄NOD小鼠作为对照.颈椎脱臼法处死两组所有小鼠.摘除眼球,钝性分离玻璃体、视网膜、巩膜.采用蛋白质免疫印迹（Wesrern blot）和实时定量逆转录聚合酶链反应（RT-PCR）对比分析实验组和对照组玻璃体、视网膜、巩膜中opticin蛋白及其相关基因（OPTC）mRNA的表达.结果 Western blot检测显示,实验组和对照组玻璃体、视网膜及巩膜中均可见opticin蛋白表达条带,主要位于相对分子质量60×10~3处.实验组玻璃体、视网膜中opticin蛋白较对照组显著降低（t=4.42,4.58 P=0.002,0.002）,巩膜中opticin蛋白差异无统计学意义（t=0.27,P=0.794）.RT-PCR检测显示,OPTC mRNA表达分布规律为玻璃体、视网膜、巩膜依次递减.实验组玻璃体、视网膜中OPTC mRNA表达较对照组显著降低（t=3.30,2.48 P=0.01,0.04）巩膜中OPTC mRNA表达差异无统计学意义（t=0.27,P=0.80）.结论糖尿病NOD小鼠玻璃体、视网膜中opticin蛋白表达较未发病NOD小鼠受抑制.

关键词：糖尿病视网膜病变/病理生理学基因表达/遗传学糖尿病,实验性

来源：评论

学校读者我要写书评

暂无评论

Peptide-oligonucleotide phosphorothioate conjugates with membrane translocation and nuclear localization properties

引用

BIOCONJUGATE CHEMISTRY 1999年第4期10卷 598-606页

作者： Antopolsky, M Azhayeva, E Tengvall, U Auriola, S Jääskeläinen, I Rönkkö, S Honkakoski, P Urtti, A Lönnberg, H Azhayev, A Univ Kuopio Dept Pharmaceut Chem FIN-70211 Kuopio Finland Univ Kuopio Dept Pharmaceut FIN-70211 Kuopio Finland Univ Turku Dept Chem FIN-20014 Turku Finland

Eighteen peptide - oligonucleotide phosphorothioate conjugates were prepared in good yield and thoroughly characterized with electrospray ionization mass spectra. When applied to the living cells, conjugates exhibiting membrane translocation and nuclear localization properties displayed efficient intracellular penetration but failed to show any serious antisense effect. Studies on the intracellular distribution of the fluorescein-labeled conjugates revealed their trapping in endosomes.

关键词：细胞膜/化学细胞核/化学交联试剂二硫化物类/化学电泳聚丙烯酰氨凝胶荧光素-5-异硫氰酸盐基因表达/遗传学水解作用萤光素酶类/生物合成萤光素酶类/遗传学质谱分析法显微镜检查共焦寡核苷酸类/化学合成寡核苷酸类/化学寡核苷酸类/代谢寡核苷酸类反义/化学合成寡核苷酸类反义/化学寡肽类/化学合成寡肽类/化学寡肽类/代谢有机硫磷化合物/化学合成有机硫磷化合物/化学有机硫磷化合物/代谢

来源：评论

学校读者我要写书评

暂无评论

In vivo gene transfer by low-volume jet injection

引用

ANALYTICAL BIOCHEMISTRY 2000年第2期282卷 262-265页

作者： Cartier, R Ren, SXV Walther, W Stein, U Lewis, A Schlag, PM Li, ML Furth, PA Univ Maryland Sch Med Ctr Med Biotechnol Inst Human Virol Baltimore MD 21201 USA Univ Maryland Sch Med Ctr Med Biotechnol Dept Med Baltimore MD 21201 USA Univ Maryland Sch Med Ctr Med Biotechnol Dept Physiol Baltimore MD 21201 USA Max Delbruck Ctr Mol Med Dept Surg & Surg Oncol D-13092 Berlin Germany

来源：评论

学校读者我要写书评

暂无评论

The genomic action potential

引用

NEUROBIOLOGY OF LEARNING AND MEMORY 2000年第3期74卷 185-216页

作者： Clayton, DF Univ Illinois Beckman Inst Neuronal Pattern Anal Grp Dept Cell & Struct Biol Urbana IL 61801 USA Univ Illinois Neurosci Program Urbana IL 61801 USA

Neurons compute in part by integrating, on a time scale of milliseconds, many synaptic inputs and generating a digital output-the "action potential" of classic electrophysiology, Recent discoveries indicate that neurons also perform a second, much slower, integration operating on a time scale of minutes or even hours. The output of this slower integration involves a pulse of gene expression which may be Likened to the electrophysiological action potential. Its function, however, is not directed toward immediate transmission of a synaptic signal but rather toward the experience-dependent modification of the underlying synaptic circuitry. Commonly termed the "immediate early gene" (IEG) response, this phenomenon is often assumed to be a necessary component of a linear, deterministic cascade of memory consolidation. Critical review of the large literature describing the phenomenon, however, leads to an alternative model of IEG function in the brain. In this alternative, IEG activation is not directed at the consolidation of memories of a specific inducing event;instead, it sets the overall gain or efficiency of memory formation and directs it to circuits engaged by behaviorally significant contexts. The net result is a sharpening of the selectivity of memory formation, a recruitment of temporally correlated associations, and an ultimate enhancement of long-term memory retrieval. (C) 2000 Academic Press.

关键词：动作电位/生理学结合部位基因表达/遗传学基因组神经元/生理学第二信使系统/遗传学人类

来源：评论

学校读者我要写书评

暂无评论

Multivariate measurement of gene expression relationships

引用

GENOMICS 2000年第2期67卷 201-209页

作者： Kim, SC Dougherty, ER Chen, YD Sivakumar, K Meltzer, P Trent, JM Bittner, M Natl Human Genome Res Inst Canc Genet Branch NIH Bethesda MD 20892 USA Texas A&M Univ Dept Elect Engn College Stn TX 77843 USA Washington State Univ Dept Elect Engn Pullman WA 99164 USA

The operational activities of cells are based on an awareness of their current state, coupled to a programmed response to internal and external cues in a context-dependent manner. One key goal of functional genomics is to develop analytical methods for delineating the ways in which the individual actions of genes are integrated into our understanding of the increasingly complex systems of organelle, cell, organ, and organism. This paper describes a novel approach to assess the codetermination of gene transcriptional states based upon statistical evaluation of reliably informative subsets of data derived from large-scale simultaneous gene expression measurements with cDNA microarrays. The method finds associations between the expression patterns of individual genes by determining whether knowledge of the transcriptional levels of a small gene set can be used to predict the associated transcriptional state of another gene. To test this approach for identification of the relevant contextual elements of cellular response, we have modeled our approach using data from known gene response pathways including ionizing radiation and downstream targets of inactivating gene mutations. This approach strongly suggests that evaluation of the transcriptional status of a given gene(s) can be combined with data from global expression analyses to predict the expression level of another gene. With data sets of the size currently available, this approach should be useful in finding sets of genes that participate in particular biological processes. As larger data sets and more computing power become available, the method can be extended to validating and ultimately identifying biologic (transcriptional) pathways based upon large-scale gene expression analysis. (C) 2000 Academic Press.

关键词：周期素依赖激酶抑制剂p21 细胞周期蛋白类/遗传学 DNA 互补/遗传学基因表达/遗传学基因表达调控/辐射效应多元分析核蛋白质类寡核苷酸序列分析原癌基因蛋白质类/遗传学原癌基因蛋白质c-mdm2 肿瘤抑制蛋白质p53/遗传学

来源：评论

学校读者我要写书评

暂无评论

Exogenous Ca²⁺-ATPase isoform effects on Ca²⁺ transients of embryonic chicken and neonatal rat cardiac myocytes

引用

JOURNAL OF PHYSIOLOGY-LONDON 2000年第1期528卷 53-63页

作者： Cavagna, M O'Donnell, JM Sumbilla, C Inesi, G Klein, MG Univ Maryland Sch Med Dept Biochem & Mol Biol Baltimore MD 21201 USA

1. Sarco-endoplasmic reticulum Ca2+-ATPase from fast skeletal (SERCA1) or cardiac muscle (SERCA2a) was expressed in embryonic chicken and neonatal rat cardiac myocytes by adenovirus vectors, with c-1. myc tags on both constructs to compare expression and distinguish exogenous from endogenous SERCA2a in myocytes. 2. Expression of the two isoforms was similar (approximately 3-fold higher than endogenous SERCA). However, SERCA1 activity wax 2-fold greater than SERCA2a activity, due to intrinsic differences in turnover rates. Activation of both exogenous SERCA isoforms by Ca2+ was displaced to slightly lon er [Ca2+], suggesting that the overexpressed isoforms were independent of phospholamban. In fact, phospholamban and calsequestrin expression were unchanged. 3. Decay time constants of cytosolic Ca2+ transients from cells overexpressing SERCA1 were reduced by 30-40% and half-widths by 10-15% compared to controls. SERCA2a overexpression produced much less acceleration of transients in chick than in rat, and less acceleration than SERCA1 overexpression in either species. There was no significant change in resting [Ca2+], peak amplitudes, or in the amount of Ca2+ releasable by: caffeine from overexpression of either SERCA isoform. However, the amplitudes of the transients increased with SERCA1 overexpression when pacing frequency limited refilling of the sarcoplasmic reticulum. 4. It is concluded that total SERCA transport velocity has a primary effect on the decay phase of transients. Transport velocity is affected by SERCA isoform turnover rate, temperature, and/or SERCA copy number.

关键词：动物新生咖啡因/药理学钙/代谢钙/药代动力学钙结合蛋白质类/生物合成钙转运ATP酶类/遗传学钙转运ATP酶类/代谢肌集钙蛋白/生物合成细胞区室化细胞培养的胞质溶胶/代谢荧光染料基因剂量基因表达/遗传学遗传载体/遗传学离子转运/药物作用同工酶类/遗传学同工酶类/代谢心肌/细胞学心肌/代谢反应时间/生理学结果可重复性肌浆网钙转运ATP酶类温度转染动物鸡胚大鼠

来源：评论

学校读者我要写书评

暂无评论

Knowledge-based analysis of microarray gene expression data by using support vector machines

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2000年第1期97卷 262-267页

作者： Brown, MPS Grundy, WN Lin, D Cristianini, N Sugnet, CW Furey, TS Ares, M Haussler, D Univ Calif Santa Cruz Dept Comp Sci Santa Cruz CA 95064 USA Univ Calif Santa Cruz Ctr Mol Biol RNA Dept Biol Santa Cruz CA 95064 USA Columbia Univ Dept Comp Sci New York NY 10025 USA Univ Bristol Dept Engn Math Bristol BS8 1TR Avon England

We introduce a method of functionally classifying genes by using gene expression data from DNA microarray hybridization experiments. The method is based on the theory of support vector machines (SVMs). SVMs are considered a supervised computer learning method because they exploit prior knowledge of gene function to identify unknown genes of similar function from expression data. SVMs avoid several problems associated with unsupervised clustering methods, such as hierarchical clustering and self-organizing maps. SVMs have many mathematical features that make them attractive for gene expression analysis, including their flexibility in choosing a similarity function, sparseness of solution when dealing with large data sets, the ability to handle large feature spaces, and the ability to identify outliers. We test several SVMs that use different similarity metrics, as well as some other supervised learning methods, and find that the SVMs best identify sets of genes with a common function using expression data. Finally, we use SVMs to predict functional roles for uncharacterized yeast ORFs based on their expression data.

关键词：算法计算机 DNA/分析数据库事实型真菌蛋白质类/分类真菌蛋白质类/遗传学基因表达/遗传学基因真菌/遗传学核酸杂交开放读码框架/遗传学酿酒酵母菌/遗传学

来源：评论

学校读者我要写书评

暂无评论

Heat shock protein 101 plays a crucial role in thermotolerance in arabidopsis

引用

PLANT CELL 2000年第4期12卷 479-492页

作者： Queitsch, C Hong, SW Vierling, E Lindquist, S Univ Chicago Dept Mol Genet & Cell Biol Howard Hughes Med Inst Chicago IL 60615 USA Univ Arizona Dept Biochem Tucson AZ 85721 USA

Plants are sessile organisms, and their ability to adapt to stress is crucial for survival in natural environments. Many observations suggest a relationship between stress tolerance and heat shock proteins (HSPs) in plants, but the roles of individual HSPs are poorly characterized. We report that transgenic Arabidopsis plants expressing less than usual amounts of HSP101, a result of either antisense inhibition or cosuppression, grew at normal rates but had a severely diminished capacity to acquire heat tolerance after mild conditioning pretreatments. The naturally high tolerance of germinating seeds, which express HSP101 as a result of developmental regulation, was also profoundly decreased. Conversely, plants constitutively expressing HSP101 tolerated sudden shifts to extreme temperatures better than did vector controls. We conclude that HSP101 plays a pivotal role in heat tolerance in Arabidopsis. Given the high evolutionary conservation of this protein and the fact that altering HSP101 expression had no detrimental effects on normal growth or development, one should be able to manipulate the stress tolerance of other plants by altering the expression of this protein.

关键词：拟南芥属/遗传学拟南芥属/生长和发育拟南芥属/生理学印迹法蛋白质保守序列/遗传学 DNA 反义/遗传学基因表达/遗传学基因必要/遗传学基因植物/遗传学萌芽/遗传学萌芽/生理学热休克蛋白质类/遗传学热休克蛋白质类/代谢热休克反应/遗传学热休克反应/生理学下胚轴/遗传学下胚轴/生长和发育下胚轴/生理学表型植物蛋白质类/遗传学植物蛋白质类/代谢植物基因修饰种子/遗传学种子/生长和发育种子/生理学时间因素转录因子/遗传学转录因子/代谢

来源：评论

学校读者我要写书评

暂无评论

Olfaction in Drosophila

引用

CURRENT OPINION IN NEUROBIOLOGY 2000年第4期10卷 498-503页

作者： Vosshall, LB Columbia Univ Ctr Neurobiol & Behav HHSC New York NY 10032 USA

The fruit fly, Drosophila melanogaster, is equipped with a sophisticated olfactory sensory system that permits it to recognize and discriminate hundreds of discrete odorants. The perception of these odorants is essential for the animal to identify relevant food sources and suitable sites for egg-laying. Advances in the last year have begun to define the molecular basis of this insect's discriminately power. The identification of a large multi-gene family of candidate Drosophila odorant receptors suggests that, as in other animals, a multitude of distinct odorants is recognized by a diversity of ligand-binding receptors. How olfactory signals are transduced and interpreted by the brain remains an important question for future analysis. The availability of genetic tools and a complete genome sequence makes Drosophila a particularly attractive organism for studying the molecular basis of olfaction.

关键词：果蝇属/生理学基因表达/遗传学基因组嗅觉受体神经元/生理学受体有气味性物质/遗传学嗅觉/遗传学动物

来源：评论

学校读者我要写书评

暂无评论

Observing the living genome

引用

CURRENT OPINION IN GENETICS & DEVELOPMENT 1999年第6期9卷 715-722页

作者： Ferea, TL Brown, PO Stanford Univ Dept Genet Sch Med Stanford CA 94305 USA Stanford Univ Howard Hughes Med Inst Sch Med Stanford CA 94305 USA Stanford Univ Dept Biochem Sch Med Stanford CA 94305 USA

Dynamic pictures of living genomes are now beginning to emerge from systematic studies of gene expression patterns using DNA microarrays. The rich information represented in the variation in each gene's expression... 详细信息

关键词：计算生物学进化分子基因表达/遗传学基因表达谱/方法基因表达调控发育期基因组肿瘤/遗传学寡核苷酸序列分析/方法动物人类

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：