The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into *** BL21(DE3).High level expression of the specific protein was achieved by IPTG *** results of SDS P...
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The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into *** BL21(DE3).High level expression of the specific protein was achieved by IPTG *** results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a.
以生物学测定和酶联检测鉴定出的大麦黄矮病毒 GAV 株系为材料,研究了病毒在麦二叉蚜体内的运行途径。在麦二叉蚜不同组织的超薄切片中,只有在唾液附腺组织中观察到膜包被的病毒粒体,在后肠腔和血淋巴中的病毒粒体是游离的。说明病毒与...
详细信息
以生物学测定和酶联检测鉴定出的大麦黄矮病毒 GAV 株系为材料,研究了病毒在麦二叉蚜体内的运行途径。在麦二叉蚜不同组织的超薄切片中,只有在唾液附腺组织中观察到膜包被的病毒粒体,在后肠腔和血淋巴中的病毒粒体是游离的。说明病毒与唾液附腺膜上传毒蛋白(受体)的相互识别是介导传播的根本原因,而麦蚜的获毒过程不存在专化的识别过程。
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