为对犬弓首蛔虫(***)免疫抑制卵巢信息蛋白(Tc-iom)、卵黄原蛋白(Tc-vit)、酪氨酸蛋白激酶(Tctpk)和丝/苏氨酸蛋白磷酸酶(Tc-stp)四个性别相关基因进行差异表达分析。本研究采用SYBR Green I qRT-PCR方法检测Tc-vit、Tc-iom、Tc-tpk、Tc...
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为对犬弓首蛔虫(***)免疫抑制卵巢信息蛋白(Tc-iom)、卵黄原蛋白(Tc-vit)、酪氨酸蛋白激酶(Tctpk)和丝/苏氨酸蛋白磷酸酶(Tc-stp)四个性别相关基因进行差异表达分析。本研究采用SYBR Green I qRT-PCR方法检测Tc-vit、Tc-iom、Tc-tpk、Tc-stp在***雌虫卵巢、输卵管、子宫和雄虫精巢、贮精囊、输精管的转录情况。结果显示,Tc-vit在雌虫卵巢、输卵管和子宫中均有转录,在雄虫精巢和贮精囊中也有转录,但在输精管中无转录;Tc-iom在雌虫卵巢、输卵管和子宫中高水平转录,在雄虫精巢和贮精囊中转录量较低,输精管中无转录;Tc-tpk在雄虫精巢和贮精囊中的转录明显高于其他生殖组织;Tc-stp在雄虫精巢和输精管中转录量较高,在雌虫卵巢、输卵管和子宫中转录量较低。本试验为***性别相关基因的进一步研究奠定了基础。
Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis *** 150 differentially expressed cDNA fragme...
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Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis *** 150 differentially expressed cDNA fragments were obtained,49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among *** fragments displayed high homology(96.1%,99.6% and 100.0%) to phosphatidylinositol transfer-like protein IV(Sec14p protein),TAC clone K9I9 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana,while alignment with NCBI databases using BLAST *** was speculated that these fragments might relate to disease-resistance of Arabidopsis to ***.
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