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Expression of receptor for ecotropic murine leukemia virus on hematopoietic cells

ARCHIVES OF VIROLOGY 2001年第3期146卷 507-519页

作者： Suzuki, T Aizawa, S Ikeda, H Natl Inst Anim Hlth Immunogenet Lab Tsukuba Ibaraki 3050856 Japan Natl Inst Radiol Sci Div Radiobiol & Biodosimetry Chiba 260 Japan Natl Inst Anim Hlth Lab Virol Prod Tsukuba Ibaraki 305 Japan

A fusion protein (F-SU/GFP) which is comprised of the surface (SU) subunit of the Friend MuLV envelope glycoprotein and the green fluorescence protein (GFP) was generated by a baculovirus expression system. The F-SU/GFP specifically bound to mammalian tissue cultured cells expressing the mCAT-1, the receptor for ecotropic murine leukemia virus (Eco-MuLV). The expression level of mCAT-1 on hematopoietic cells was measured based on the capacity of cells to absorb the F-SU/GFP. In BALB/c mice susceptible to Eco-MuLV infection, all hematopoietic cell subpopulations absorbed the F-SU/GFP with the most prominent absorption observed in the TER119(+) erythroblasts. Hematopoietic cells from C4W and AKR mice did not absorb the F-SU/GFP as readily as the BALB/c cells, probably due to expression of the envelope glycoproteins of endogenous Eco-MuLVs in these mice. Although AKR mice are susceptible to Eco-MuLV infection while C4W mice are resistant, these mice showed no apparent difference in the F-SU/GFP-absorbing capacity.

关键词：氨基酸转运系统碱性杆状病毒科/遗传学载体蛋白质类/代谢细胞系幼红细胞/代谢弗林德鼠白血病病毒/代谢遗传载体膜糖蛋白类/代谢膜蛋白质类/代谢小鼠近交AKR 小鼠近交BALBC 蛋白质结合受体病毒/代谢重组蛋白质类/代谢病毒包膜蛋白质类/遗传学病毒包膜蛋白质类/代谢病毒融合蛋白质类/代谢动物小鼠

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HOST RESTRICTION OF FRIEND LEUKEMIA-VIRUS - SYNTHESIS AND INTEGRATION OF PROVIRUS

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1976年第7期73卷 2356-2360页

作者： SVEDA, MM SOEIRO, R YESHIVA UNIV ALBERT EINSTEIN COLL MED DEPT MED BRONX NY 10461 USA YESHIVA UNIV ALBERT EINSTEIN COLL MED DEPT CELL BIOL BRONX NY 10461 USA

Host restriction of exogenous infection by murine leukemia viruses is controlled in vitro predominantly by the murine Fv-1 locus. The mechanism of this host restriction was investigated by comparing the early events in the replication of N-tropic vs. B-tropic Friend leukenia virus in [mouse fibroblast] NIH 3T3 cells. These cells, which are Fv-1nn in type, are permissive for the N-tropic strain, but nonpermissive for the B-tropic strain, which replicates permissively in [mouse fibroblast] Balb/c 3T3 cells. The synthesis, intracellular location and molecular form of virus-specific DNA early in replication was studied using molecular hybridization with a virus-specific DNA probe. In the permissive infection, viral DNA rapidly becomes integrated with cellular DNA. In the nonpermissive infection, although almost equal amounts of positive and negative strand viral DNA are synthesized, integration of the provirus does not occur.

关键词：细胞系 DNA/代谢 DNA 环状/分析 DNA 病毒/分析 DNA 病毒/生物合成 DNA 病毒/代谢弗林德鼠白血病病毒/代谢基因核酸构象病毒复制

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PURINE METABOLISM IN MURINE VIRUS-INDUCED ERYTHROLEUKEMIC CELLS DURING DIFFERENTIATION INVITRO

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1975年第4期72卷 1630-1634页

作者： REEM, GH FRIEND, C NYU SCH MEDDEPT PHARMACOLNEW YORKNY 10016 CITY UNIV NEW YORK MT SINAI SCH MEDCTR EXPTL CELL BIOLMOLLIE B ROTH LABNEW YORKNY 10029

Purine metabolism was studied in murine virus-induced erythroleukemia cells stimulated to differentiate in vitro in the presence of dimethylsulfoxide. The activities of the enzymes that catalyze the synthesis of the first intermediate of the de novo purine pathway, phosphoribosyl-1-amine, were decreased while the enzymes that catalyze the conversion of purine bases to purine ribonucleotides remained unchanged at the time the cells acquired the specialized function of hemoglobin synthesis. In addition, cytidine deaminase (cytidine aminohydrolase, EC 3.5.4.5) activity increased with erythropoietic maturation, as it does during murine erythropoiesis in vivo. Stimulation of cellular proliferation of stationary erythroleukemic cells resulted in a marked increase in the activities of purine biosynthetic enzymes. These data provide a convincing example of repression and derepression of the PRA synthesizing enzymes in mammalian cells in vitro, and further evidence that the regulatory mechanisms operative in the normal development of erythrocytes can be activated by exposure of erythroleukemic cells to dimethylsulfoxide.

关键词：腺嘌呤/代谢酰胺磷酸核糖基转移酶/代谢氨细胞系细胞转化肿瘤色谱法薄层克隆细胞胞苷脱氨酶/代谢二甲亚砜/药理学弗林德鼠白血病病毒/代谢次黄嘌呤类/代谢白血病实验性/代谢连接酶类/代谢嘌呤类/代谢一磷酸核糖时间因素动物小鼠

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DISTRIBUTION AND VIROGENIC EFFECTS OF 5-BROMODEOXYURIDINE IN SYNCHRONIZED RAT EMBRYO CELLS

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1975年第5期72卷 1829-1833页

作者： SCHWARTZ, SA PANEM, S KIRSTEN, WH UNIV CHICAGO PRITZKER SCH MEDDEPT PATHOLCHICAGOIL 60637 UNIV CHICAGO PRITZKER SCH MEDJOSEPH P KENNEDY JR MENTAL RETARDATION RES CTRCHICAGOIL

Rat embryo cell cultures were synchronized by a double thymidine block. The DNA replication phase (S) was divided into an early, middle, and late period. Cell cultures in the early, middle, or late S phase were pulsed with 0.1 muM 5-bromo[(3)H]deoxyuridine (BrdU) or equimolar [(3)H]dT. DNA-DNA reassociation experiments of each sample revealed that [(3)H]BrdU was more concentrated in the intermediate repetitive than the repetitive or unique DNA sequences of the early and middle S phase. In contrast, [(3)H]dT was nearly uniformly jistributed throughout all nucleotide sequences during the entire S phase. synchronized rat cells were pulsed during various portions of the S phase with unlabeled 0.1 mM or 0.1 muM BrdU and examined for sytoplasmic immumofluorescence against the 30,000 molecular weight group-specific antigen (p30) of Friend mouse leukemia virus. Equally strong fluorescence was detected 12 hr later in cells treated with each concentration of BrdU. Furthermore, incorporation of BrdU during late S phase was suffieient to elicit maximal antigen expression.

关键词：抗原病毒/分析溴脱氧尿苷/药理学细胞分裂/药物作用色谱法 DNA/生物合成 DNA复制/药物作用弗林德鼠白血病病毒/免疫学弗林德鼠白血病病毒/代谢动力学核酸复性大鼠/胚胎学病毒复制/药物作用动物

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ERYTHROID COLONY INDUCTION WITHOUT ERYTHROPOIETIN BY FRIEND LEUKEMIA-VIRUS INVITRO

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1975年第9期72卷 3556-3560页

作者： CLARKE, BJ AXELRAD, AA SHREEVE, MM MCLEOD, DL UNIV TORONTO FAC MEDDEPT ANATDIV HISTOLTORONTO M5S 1A8ONTARIOCANADA

Erythroid colonies could be produced without the addition of erythropeietin in plasma cultures seeded with bone marrow cells from normal C3Hf/Bi mice by exposure of the cells in vitro to medium from a cell line (IS) that continuously produces Friend leukemia virus in culture. The activity in the culture medium was viral rather than erythropoietin-like, since it was sedimentable by high-speed centrifugation and heat labile. Erythroid colonies did not develop when the bone marrow cells exposed to virus-containing medium were from mice genetically resistant to Friend virus. IS culture medium contained both Friend spleen focus-forming and XC-plaque-forming activities. No erythroid colonies were induced when genetically sensitive cells were exposed to a preparation from which the spleen focus-forming activity had been removed, but which contained XC plaque-forming activity in high concentration. Thus the spleen focus-forming component of Friend virus appeared to be responsible for inducing erythroid colony formation without erythropoietin in vitro. Some erythroid colonies were also found in control cultures to which neither virus nor erythropoietin had been added. Reduction in the concentration of fetal calf serum in the culture medium substantially decreased the number of these colonies but had only a minor effect on the number of virus-induced colonies. The number of erythroid colonies produced after 2 days of culture without erythropoietin or fetal calf serum was approximately proportional to the titer of Friend spleen focus-forming virus to whcih the bone marrow cells had been exposed. This system should prove useful for investigation in vitro of Friend virus--host cell interactions which lead to erythropoietin-independent erythropoiesis.

关键词：骨髓/代谢骨髓/微生物学骨髓细胞牛/血液细胞系红细胞生成红细胞生成素弗林德鼠白血病病毒/代谢小鼠近交BALBC 小鼠近交C3H 小鼠近交系种特异性动物小鼠

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