This study reports the presence of oval-shaped pores in the basement membrane of the human bronchial airway that may be used as conduits for immune cells to traffic between the epithelial and mesenchymal compartments....
详细信息
This study reports the presence of oval-shaped pores in the basement membrane of the human bronchial airway that may be used as conduits for immune cells to traffic between the epithelial and mesenchymal compartments. Human bronchial mucosa collected after surgery was stripped of epithelial cells without damaging the basement membrane. Both scanning and transmission electron microscopy showed oval-shaped pores 0.75 to 3.85 mum in diameter in the bronchial basement membrane at a density of 863 pores/ mm(2). Transmission electron microscopy showed that the pores spanned the full depth of the basement membrane, with a concentration of collagen-like fibers at the lateral edges of the pore. Infiltrating cells apparently moved through the pores, both in the presence and absence of the epithelium. Taken together, these results suggest that immune cells use basement membrane pores as predefined routes to move between the epithelial and mesenchymal compartments without disruption of the basement membrane. As a persistent feature of the basement membrane, pores could facilitate inflammatory cell access to the epithelium and greatly increase the frequency of intercellular contact between trafficking cells.
Each of six calves inoculated endobronchially with bovine herpesvirus 1 (BHV-1) by means of a bronchoscope developed viral pneumonia. Gross and histopathological lesions were mainly localized to the right diaphragmati...
详细信息
Each of six calves inoculated endobronchially with bovine herpesvirus 1 (BHV-1) by means of a bronchoscope developed viral pneumonia. Gross and histopathological lesions were mainly localized to the right diaphragmatic lobe (middle to caudal region) of the lung and were closely associated with the site of the deposition of the inoculum. The lesions were characterized by intranuclear inclusion bodies associated with focal necrosis of the epithelium in the lower respiratory tract. BHV-1 antigen and BHV particles were detected in the degenerating bronchial, bronchiolar and alveolar epithelial cells. After infection, the total cell count in the broncho-alveolar lavage (BAL) fluid increased. In addition, BHV-1 antigen and virus were detected in the desquamated cells and macrophages of BAL fluid from the right diaphragmatic lobe, but not from the left diaphragmatic lobe. It is concluded that examination of BAL fluid is valuable for immunohistopathological and virological confirmation of BHV-1 infection. (C) 2000 Harcourt Publishers Ltd.
The presence of actin has been demonstrated in ciliated cells of the lower respiratory tract by means of immunological techniques and electron microscopy. This protein may play a part in ciliary movement and in its co...
详细信息
The presence of actin has been demonstrated in ciliated cells of the lower respiratory tract by means of immunological techniques and electron microscopy. This protein may play a part in ciliary movement and in its coordination.
暂无评论