检索结果-内蒙古大学图书馆

In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2000年第16期97卷 9191-9196页

作者： Schechner, JS Nath, AK Zheng, L Kluger, MS Hughes, CCW Sierra-Honigmannn, MR Lorber, MI Tellides, G Kashgarian, M Bothwell, ALM Pober, JS Yale Univ Sch Med Dept Dermatol Interdepartmental Program Vasc Biol & Transplanta New Haven CT 06520 USA Yale Univ Sch Med Immunobiol Sect New Haven CT 06520 USA Yale Univ Sch Med Dept Pathol New Haven CT 06520 USA Yale Univ Sch Med Dept Surg New Haven CT 06520 USA

We have identified conditions for forming cultured human umbilical vein endothelial cells (HUVEC into tubes within a three-dimensional gel that on implantation into immunoincompetent mice undergo remodeling into complex microvessels lined by human endothelium, HUVEC suspended in mixed collagen/ fibronectin gels organize into cords with early lumena by 24 h and then apoptose, Twenty-hour constructs, s,c, implanted in immunodeficient mice, display HUVEC-lined thin-walled microvessels within the gel 31 days after implantation. Retroviral-mediated overexpression of a caspase-resistant Bcl-2 protein delays HUVEC apoptosis in vitro for over 7 days. Bcl-2-transduced HUVEC produce an increased density of HUVEC-lined perfused microvessels in vivo compared with untransduced or control-transduced HUVEC. Remarkably, Bcl-2- but not control-transduced HUVEC recruit an ingrowth of perivascular smooth-muscle alpha-actin-expressing mouse cells at 31 days, which organize by 60 days into HUVEC-lined multilayered structures resembling true microvessels, This system provides an in vivo model for dissecting mechanisms of microvascular remodeling by using genetically modified endothelium, Incorporation of such human endothelial-lined microvessels into engineered synthetic skin may improve graft viability, especially in recipients with impaired angiogenesis.

关键词：毛细血管/细胞学毛细血管/超微结构细胞培养的共同培养技术内皮血管/细胞学内皮血管/超微结构小鼠 SCID 显微镜检查电子动物人类小鼠

来源：评论

学校读者我要写书评

暂无评论

Signaling via fibroblast growth factor receptor-1 is dependent on extracellular matrix in capillary endothelial cell differentiation

引用

EXPERIMENTAL CELL RESEARCH 1999年第1期248卷 203-213页

作者： Kanda, S Tomasini-Johansson, B Klint, P Dixelius, J Rubin, K Claesson-Welsh, L Biomed Ctr Dept Med Biochem & Microbiol S-75123 Uppsala Sweden

Differentiation of endothelial cells, i.e., formation of a vessel lumen, is a prerequisite for angiogenesis. The underlying molecular mechanisms are ill defined. We have studied a brain capillary endothelial cell line (IBEC) established from H-2K(b)-tsA58 transgenic mice. These cells form hollow tubes in three-dimensional type I collagen gels in response to fibroblast growth factor-2 (FGF-2). Culture of IBEC on collagen gels in the presence of FGF-S protected cells from apoptosis and allowed tube formation (i.e., differentiation) but not growth of the cells. FGF-induced differentiation, but not cell survival, was inhibited by treatment of the cells with an anti-beta(1)-integrin IgG. Changes in integrin expression in the collagen-gel cultures could not be detected. Rather, cell-matrix interactions critical for endothelial cell differentiation were created during the culture, as indicated by the gradual increase in tyrosine phosphorylation of focal adhesion kinase in the collagen-gel cultures. Inclusion of laminin in the collagen gels led to FGF-2-independent formation of tube structures, but cells were not protected from apoptosis. These data indicate that FGF receptor-1 signal transduction in this cell model results in cell survival. Through mechanisms dependent on cell-matrix interactions, possibly involving the alpha(3)beta(1)-integrin and laminin produced by the collagen-cultured IBE cells, FGF stimulation also leads to differentiation of the cells. (C) 1990 Academic Press.

关键词：氨基酸序列抗原 CD29/代谢细胞凋亡毛细血管/细胞学毛细血管/代谢细胞黏附分子/代谢细胞分化细胞系胶原内皮血管/细胞学细胞外基质/代谢成纤维细胞生长因子1 成纤维细胞生长因子2/药物作用黏着斑激酶1 黏着斑蛋白酪氨酸激酶类整合素类/生物合成层黏连蛋白/代谢分子序列数据磷酰化蛋白酪氨酸激酶类/代谢受体蛋白质酪氨酸激酶类/代谢受体成纤维细胞生长因子 1型受体成纤维细胞生长因子/代谢信号传导酪氨酸/代谢动物小鼠

来源：评论

学校读者我要写书评

暂无评论

Effect of indomethacin on capillary growth and microvasculature in chronically stimulated rat skeletal muscles

引用

JOURNAL OF PHYSIOLOGY-LONDON 2000年第2期526卷 435-443页

作者： Pearce, SC Hudlická, O Brown, MD Univ Birmingham Sch Med Dept Physiol Birmingham W Midlands England Univ Birmingham Sch Med Sch Sport & Exercise Sci Birmingham W Midlands England

1. Capillary proliferation and microvessel diameters were studied in rat ankle flexors subjected to chronic electrical stimulation by implanted electrodes (10 Hz, 0.3 ms pulse width, up to 6 V, 8 h day(-1)) for 2 or 7 days with or without concurrent indomethacin treatment (similar to 2 mg day(-1) in drinking water) to study the role of prostaglandins in the microcirculation in relation to capillary growth. *** of terminal arterioles, capillaries and confluent venules were measured in epi-illuminated muscles, together with capillary red cell velocity, to evaluate whether changes in capillary pressure and/or shear stress participate in capillary growth via release of prostaglandins. 3. Cell proliferation was detected following bromodeoxyuridine (BrdU) incorporation and immune-staining of frozen sections. Labelling was assessed as the percentage of all interstitial nuclei (Haematoxylin-stained) that were BrdU positive. By comparison with serial sections stained for alkaline phosphatase, from which the capillary-to-fibre ratio (C:F) was obtained, labelling was derived for nuclei colocalised either to capillaries or to other non-capillary interstitial cells. 4. C:F increased to 1.89 +/- 0.06 from 1.47 +/- 0.04 in controls only after 7 days stimulation;indomethacin reduced this to 1.55 +/- 0.07. Capillary labelling increased from 2.9 +/- 0.5% in controls to 11.3 +/- 2.2 % after 2 days stimulation and 10.6 +/- 0.8% after 7 days. The increase was attenuated by indomethacin at both time points (to 5.8 +/- 1.6% and 4.2 +/- 0.5%, respectively). 5. Non-capillary interstitial labelling (2.0 +/- 0.4% in controls) increased to 9.5 +/- 2.7% after 2 days stimulation and was back to normal after 7 days (3.2 +/- 0.07 %). Indomethacin depressed the increase at 2 days to 4.0 +/- 1.3% and had no effect at 7 days (2.9 +/- 0.13%). Labelling in sham-operated rats with or without indomethacin or in vehicle-treated animals was no different from controls. 6. Arteriolar and venular diam

关键词：小动脉/细胞学小动脉/药物作用小动脉/生理学毛细血管/细胞学毛细血管/药物作用毛细血管/生理学细胞分裂/药物作用环加氧酶抑制药/药理学电刺激吲哚美辛/药理学微循环/细胞学微循环/药物作用微循环/生理学肌骨骼/血液供给肌骨骼/药物作用肌骨骼/生理学大鼠 Sprague-Dawley 应力物理小静脉/细胞学小静脉/药物作用小静脉/生理学动物男(雄)性大鼠

来源：评论

学校读者我要写书评

暂无评论

Ability of Ni-containing biomedical alloys to activate monocytes and endothelial cells in vitro

引用

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 1999年第3期45卷 251-257页

作者： Wataha, JC Lockwood, PE Marek, M Ghazi, M Med Coll Georgia Sch Dent Dept Oral Rehabil Augusta GA 30912 USA Georgia Inst Technol Atlanta GA 30332 USA Georgia State Univ Atlanta GA 30303 USA

Nickel-containing alloys commonly are used in medical and dental applications that place them into longterm contact with soft: tissues. The release of Ni ions from these alloys is disturbing because of the toxic, immunologic, and carcinogenic effects that have been documented for some Ni compounds. In particular, Ni ions in solution recently have been shown to cause expression of inflammatory mediators, such as interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and intercellular adhesion molecules (ICAMs) from keratinocytes, monocytes, and endothelial cells. However, the ability of the solid alloys themselves to induce these inflammatory effects has not been demonstrated. An in vitro system was used to determine if Ni-containing biomedical alloys could cause secretion of either IL-1 beta or TNF-alpha from monocytes or expression of ICAMs on endothelial cells. Pure nickel, titanium, and three biomedical alloys-18-8 stainless steel, NiTi, and Rexillium III-were evaluated. First, it was determined whether or not the alloys or pure metals could cause cytotoxicity to THP-1 human monocytes or human microvascular endothelial cells (HMVECs) by measuring the succinic dehydrogenase (SDH) activity of the cells. Then, using identical conditions of exposure, the secretion of IL-1 beta or TNP-alpha from monocytes or ICAM-1 expression on the HMVECs was determined. Only pure nickel suppressed (by 48% compared to Teflon controls) the SDH activity of the HMVECs or THT-1 monocytes. No alloy or metal caused the HMVECs to express ICAM-1, but the NiTi alloy caused a significant (ANOVA/Tukey) secretion of IL-1 beta from the THP-1 monocytes. Secretion of TNF-alpha induced by NiTi was detectable but not statistically significant. The levels of IL-1 beta secretion from monocytes were sufficient to induce ICAM-1 expression on HMVECs. The release of Ni from the NiTi was a logical suspect in causing the IL-1 beta secretion by monocytes, but its role was not confirmed since

关键词：合金/毒性毛细血管/细胞学毛细血管/药物作用细胞存活/药物作用细胞培养的内皮血管/细胞学内皮血管/药物作用内皮血管/代谢胞间黏附分子1/生物合成白细胞介素1/代谢巨噬细胞活化/药物作用单核细胞/化学单核细胞/药物作用镍/分析镍/毒性琥珀酸脱氢酶/代谢钛/毒性肿瘤坏死因子α/代谢人类

来源：评论

学校读者我要写书评

暂无评论

Physiology of rat retinal pericytes: modulation of ion channel activity by serum-derived molecules

引用

JOURNAL OF PHYSIOLOGY-LONDON 1999年第3期521卷 637-650页

作者： Sakagami, K Wu, DM Puro, DG Univ Michigan Dept Ophthalmol & Visual Sci Ann Arbor MI 48105 USA Univ Michigan Dept Physiol Ann Arbor MI 48105 USA

1. Pericytes, which are contractile cells located on the outer wall of microvessels, are thought to be particularly important in the retina where the ratio of these cells to vascular endothelial cells is the highest of any tissue. Retinal pericytes are of interest since they may regulate capillary blood flow and because their selective loss is an early event in diabetic retinopathy, which is a common sight-threatening disorder associated with dysfunction of the blood-retinal barrier. 2. Although a breakdown in the vascular endothelial barrier is a frequent pathophysiological event, knowledge of the effects of blood-derived molecules on pericyte function is limited. Based on the premise that ion channels play a vital role in cellular function, we examined the effect of serum on the ionic currents of retinal pericytes. To do this, we used the perforated patch configuration of the patch-clamp technique to monitor the whole-cell currents of pericytes located on freshly isolated rat retinal microvessels. 3. Exposure to serum reversibly activated inward and outward currents in virtually all of the sampled retinal pericytes. Two types of sustained conductances were induced by serum. These were a calcium-permeable non-specific cation (NSC) current and a voltage-dependent potassium current. In addition, exposure to serum increased the activity of chloride channels which caused transient depolarizing currents. 4. Associated with the activation of these conductances, the membrane potential showed a sustained decrease of 10 +/- 2 mV from -56 mV to -46 mV and, also, transient depolarizations to near -30 mV. The serum-induced depolarizations can activate the voltage-gated calcium channels expressed by the retinal pericytes. 5. Calcium-permeable NSC channels appear to play a critical role in the response of pericytes to serum-derived molecules. Consistent with this, activation of the chloride and potassium channels was sensitive to SK&F 98365, which is a blocker of NSC channels. I

关键词：血蛋白质类/药理学血视网膜屏障/药物作用血视网膜屏障/生理学钙通道/药物作用钙通道/生理学毛细血管/细胞学毛细血管/药物作用氯化物通道/药物作用氯化物通道/代谢电生理学离子通道闸门/药物作用离子通道闸门/生理学离子通道/药物作用离子通道/生理学微透析显微镜检查电子扫描肌收缩/药物作用肌收缩/生理学肌平滑血管/细胞学肌平滑血管/药物作用肌平滑血管/生理学膜片钳术钾通道/药物作用钾通道/代谢视网膜/细胞学视网膜/药物作用视网膜/生理学四乙基铵/药理学动物大鼠

来源：评论

学校读者我要写书评

暂无评论

ANGIOTENSIN-II - RENAL LOCALIZATION IN GLOMERULAR MESANGIAL CELLS BY AUTORADIOGRAPHY

引用

KIDNEY INTERNATIONAL 1975年第4期8卷 245-254页

作者： OSBORNE, MJ DROZ, B MEYER, P MOREL, F HOP NECKER INSERMUNIT 7PARISFRANCE COMM ENERGIE ATOM DEPT BIOLGIF SUR YVETTEFRANCE

Angiotensin II: Renal localization in glomerular mesangial cells by autoradiography. The intrarenal localization of 3H-angiotensin II after intraaortic administration in anesthetized rats has been studied by autoradio... 详细信息

Angiotensin II: Renal localization in glomerular mesangial cells by autoradiography. The intrarenal localization of ³H-angiotensin II after intraaortic administration in anesthetized rats has been studied by autoradiography. A net concentration of radioactivity is seen in the glomeruli as compared with tubules, blood vessels and interstices. Within the glomerulus this radioactivity is predominantly localized in the mesangial cells. This mesangial concentration of radioactivity is present at 1, 2 and 5 min after ³H-angiotensin administration but is considerably diminished after 30 min. Administration of a large dose of angiotensin II or of Ile⁸-angiotensin II (a competitive inhibitor of angiotensin II) with the ³H-angiotensin II prevented the mesangial concentration of radioactivity. A similar dose of noradrenaline did not have this effect. ³H-2-8-angiotensin II-heptapeptide also showed a mesangial distribution, whereas ³H-tyrosine did not. Less mesangial radioactivity after ³H-angiotensin administration was seen in rats kept on a low salt diet for four days than in those receiving a high salt diet. The possibility of an action of angiotensin II at the level of the mesangial cells is discussed.

关键词：血管紧张素Ⅱ/投药和剂量血管紧张素Ⅱ/分离和提纯血管紧张素Ⅱ/药理学放射自显影术/方法毛细血管/细胞学毛细血管/超微结构剂量效应关系药物组织学技术肾/细胞学肾小球/生理学肾小球/超微结构去甲肾上腺素/药理学放射性示踪剂钠/药理学氚动物男(雄)性大鼠

来源：评论

学校读者我要写书评

暂无评论

Interpretation of quantitative measurement of skin capillaries using native in vivo microscopy

引用

MICROVASCULAR RESEARCH 2000年第2期60卷 189-192页

作者： Lamah, M Mortimer, PS Dormandy, JA St George Hosp Sch Med Dept Surg London SW17 0RE England St George Hosp Sch Med Dept Dermatol London SW17 0RE England

Intravital native microscopy is a useful tool in the study of the microcirculation in vivo. The interpretation of photographic findings remains controversial, however, as native microscopy relies principally upon the presence of pigment in the hemoglobin contained in red blood cells. Theoretically, therefore, visible capillaries might only represent a proportion of the total number of capillaries. The aim of this study was to estimate the proportion of capillaries, which remain unseen during native microscopy and so relate the number of visible capillaries to the true total number of capillaries.

关键词：毛细血管/细胞学微循环/细胞学显微镜检查电视皮肤/血液供给成年人老年人女(雌)性人类男(雄)性中年人

来源：评论

学校读者我要写书评

暂无评论

Inhibitory effect of full-length human endostatin on in vitro angiogenesis

引用

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1999年第2期263卷 340-345页

作者： Taddei, L Chiarugi, P Brogelli, L Cirri, P Magnelli, L Raugei, G Ziche, M Granger, HJ Chiarugi, V Ramponi, G Dept Biochem Sci I-50134 Florence Italy Inst Gen Pathol I-50134 Florence Italy Dept Clin & Preclin Pharmacol I-50134 Florence Italy Texas A&M Univ Hlth Sci Ctr Cardiovasc Res Inst College Stn TX USA Texas A&M Univ Hlth Sci Ctr Dept Med Physiol College Stn TX USA

Endostatin, a C-terminal product of collagen XVIII, is a very powerful angiogenesis inhibitor. In vivo experiments in mice indicate that endostatin dramatically reduces tumor mass without causing the onset of any resistance to the treatment. Recently, a 12-aa shorter human endostatin has been purified from plasma, but is ineffective in in vitro angiogenesis assays. Here we report that the full-length human recombinant endostatin has a potent inhibitory activity in in vitro angiogenesis assays. Two powerful angiogenic factors were used to stimulate endothelial cells: FGF-2 and VEGF-165. Endostatin prevented cell growth both in the basal condition and after stimulation with FGF-2 or VEGF-165. Migration of microvascular endothelial cells toward FGF-2 or VEGF-165 was impaired, both when cells mere pretreated with the inhibitor and when endostatin was added together with the growth factors. Furthermore, experiments of inhibition of proliferation performed on nonmicroendothelial cells showed that endostatin was ineffective. This study indicates that human endostatin is a potent angiogenesis inhibitor and suggests its use in human anticancer therapy. (C) 1999 Academic Press.

关键词：毛细血管/细胞学毛细血管/药物作用细胞分裂/药物作用细胞运动/药物作用克隆分子胶原/遗传学胶原/药理学胶原ⅩⅧ型内皮抑素类内皮生长因子/药理学内皮血管/细胞学内皮血管/药物作用成纤维细胞生长因子2/药理学淋巴因子/药理学新生血管化生理性/药物作用肽碎片/遗传学肽碎片/药理学重组蛋白质类/药理学血管内皮生长因子A 血管内皮生长因子类小静脉/细胞学小静脉/药物作用人类

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：