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Lactic acid bacteria associated with the digestive tract of Atlantic salmon (Salmo salar L.)

JOURNAL OF APPLIED MICROBIOLOGY 2000年第2期89卷 317-322页

作者： Ringo, E Bendiksen, HR Wesmajervi, MS Olsen, RE Jansen, PA Mikkelsen, H Norwegian Sch Vet Sci Dept Arctic Vet Med NO-9292 Tromso Norway Univ Tromso Dept Med Microbiol Tromso Norway Finnmark Coll Dept Fisheries Alta Norway Finnmark Res Ctr Hammerfest Norway Norwegian Inst Fisheries & Aquaculture Ltd Tromso Norway

The present study reports the effect of excessive handling stress and starvation on the lactic acid bacteria associated with the digestive tract of Atlantic salmon (Salmo salar L.). A relatively low population level (approximately 2 x 10(3) bacteria per gram wet tissue) of viable adherent heterotrophic bacteria was associated with the digestive tract (foregut, midgut and hindgut). Of the 752 bacterial isolates isolated from diet, water and the digestive tract, 201 isolates belonged to the carnobacteria. Of these isolates, one from the diet, one from the rearing water and 80 from the gastrointestinal tract, were further identified on the basis of 16S rDNA sequence analysis. All these isolates were identified as being Carnobacterium piscicola-like. Daily repeated stress and starvation of the fish over 11 d had no influence on the total culturable bacterial numbers or population level of C. piscicola associated with the digestive tract. C. piscicola-like isolates colonizing the various intestinal regions (foregut, midgut and hindgut) were also screened for their ability to produce growth inhibitory compounds active against the fish pathogen Aeromonas salmonicida. Of the 199 C. piscicola isolates tested, 139 inhibited growth of the pathogen.

关键词：气单胞菌属/生长和发育气单胞菌属/分离和提纯抗生作用集落计数微生物 DNA 细菌/分析 DNA 细菌/遗传学 DNA 核糖体/分析 DNA 核糖体/遗传学消化系统/微生物学脂肪酸类/分析鱼疾病/微生物学革兰氏阴性菌感染/微生物学革兰氏阴性菌感染/兽医学乳杆菌科/化学乳杆菌科/生长和发育乳杆菌科/分离和提纯表型 RNA 核糖体 16S/遗传学盐湖鲑鱼/微生物学盐湖鲑鱼/生理学动物

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Hemolytic activity and siderophore production in different Aeromonas species isolated from fish

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY 1999年第12期65卷 5612-5614页

作者： Santos, JA González, CJ Otero, A García-López, ML Univ Leon Fac Vet Dept Higiene & Tecnol Alimentos E-24071 Leon Spain

The hemolytic activity and siderophore production of several strains of motile aeromonads were determined. The hemolytic activity of Aeromonas caviae and Aeromonas eucrenophila was enhanced after trypsinization of the samples. The enhancement of hemolysis was observed in strains that carried an aerolysin-like gene, detected by a PCR procedure. Siderophore production was demonstrated in all but one strain of Aeromonas jandaei. No apparent relationship was observed between the presence of plasmid DNA and hemolysis or siderophore production.

关键词：气单胞菌属/分离和提纯气单胞菌属/致病力气单胞菌属/生理学细菌毒素类/遗传学狗鱼科/微生物学溶血素蛋白质类/遗传学溶血大麻哈鱼裂喉/微生物学质粒聚合酶链反应成孔毒素蛋白质类绵羊铁载体/生物合成鳟/微生物学动物兔

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Monoclonal antibodies against AsaP1, a major exotoxin of the fish pathogen Aeromonas salmonicida subsp achromogenes, and their application in ELISA

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JOURNAL OF APPLIED MICROBIOLOGY 1999年第4期87卷 620-629页

作者： Wagner, U Gudmundsdóttir, BK Drössler, K Univ Leipzig Inst Zool D-04103 Leipzig Germany Univ Iceland Inst Expt Pathol Reykjavik Iceland

Two monoclonal antibodies (Mabs) binding to a toxic extracellular metallo-proteinase of Aeromonas salmonicida subsp. achromogenes, AsaP1, were produced. Both reacted with common epitopes of the native enzyme and recognized this 20 kDa antigen on Western blots. One of these Mabs had an inhibitory effect on the caseinase activity of the exotoxin. A Mab-based ELISA was set up and evaluated for serological detection of AsaP1 in bacterial culture filtrates. The exotoxin was identified serologically in the extracellular products of 11 of 26 atypical Aer. salmonicida isolates, including the type strain for subsp. achromogenes NCIMB 1110. The ELISA was approximately 100-fold more sensitive in detecting AsaP1 compared with an azocasein assay. The established serological test enables AsaP1 to be quantified reliably with a lower detection limit of about 0.12 ng ml(-1) and has a potential use for the phenotypic differentiation of atypical Aer. salmonicida isolates.

关键词：气单胞菌属/免疫学气单胞菌属/分离和提纯气单胞菌属/代谢抗体单克隆/生物合成印迹法蛋白质载体蛋白质类/化学载体蛋白质类/免疫学电泳聚丙烯酰氨凝胶酶联免疫吸附测定外毒素类/化学外毒素类/免疫学小鼠近交BALBC 沉淀素试验动物女(雌)性小鼠

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Prevalence and virulence properties of Vibrio cholerae non-O1, Aeromonas spp. and Plesiomonas shigelloides isolated from Cambe Stream (State of Parana, Brazil)

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JOURNAL OF APPLIED MICROBIOLOGY 2000年第1期89卷 70-75页

作者： Gibotti, A Saridakis, HO Pelayo, JS Tagliari, KC Falcao, DP UNESP Fac Ciencias Farmaceut Dept Ciencias Biol BR-14801902 Araraquara SP Brazil

The incidence of Vibrio cholerae, Aeromonas spp, and Plesiomonas shigelloides was determined in Rater samples from Cambe Stream. The samples were collected from seven different sites. The serogroups, virulence markers and drug resistance profiles were also evaluated. Twelve. Aer. hydrophila, 12 Aer. caviae, eight Aer. sobria, seven Ple. shigelloides and two V. cholerae non-O1 were isolated. They belonged to different serogroups and all produced haemolysis in different assays. Five of the Aeromonas strains and one of V, cholerae non-O1 were positive for enterotoxin activity. Haemagglutination and its inhibition, using erythrocytes of different origins, was variable for Aeromonas spp and V. cholerae, while none of the Plt. shigelloides haemagglutinated in association with any type of erythrocyte. All isolates exhibited multiple drug resistance. These results indicate that the occurrence of V. cholerae non-O1, Aeromonas spp, and Ple. shigelloides, in water used for vegetable irrigation, human recreation and animal consumption, among others, represents a potential risk for humans.

关键词：气单胞菌属/分类气单胞菌属/分离和提纯气单胞菌属/致病力巴西淡水/微生物学血凝集血凝集抑制试验溶血马邻单胞菌属/分类邻单胞菌属/分离和提纯邻单胞菌属/致病力霍乱弧菌/分类霍乱弧菌/分离和提纯霍乱弧菌/致病力毒力水微生物学动物豚鼠人类小鼠

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PCR detection, characterization, and distribution of virulence genes in Aeromonas spp.

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY 1999年第12期65卷 5293-5302页

作者： Bin Kingombe, CI Huys, G Tonolla, M Albert, MJ Swings, J Peduzzi, R Jemmi, T Swiss Fed Vet Off Microbiol Sect CH-3003 Liebefeld Bern Switzerland State Univ Ghent Microbiol Lab B-9000 Ghent Belgium Ist Cantonale Batteriosierol Lugano Switzerland Int Ctr Diarrhoeal Dis Res Dhaka 2 Bangladesh

We found 73.1 to 96.9% similarity by aligning the cytolytic enterotoxin gene of Aeromonas hydrophila SSU (AHCYTOEN;GenBank accession no. M84709) against aerolysin genes of Aeromonas spp,, suggesting the possibility of selecting common primers. Identities of 90 to 100% were found among the eight selected primers from those genes, Amplicons obtained from Aeromonas sp, reference strains by using specific primers for each gene or a cocktail of primers were 232 bp long. Of hybridization group 4/5A/5B (HG4/5A/5B), HG9, and HG12 or non-Aeromonas reference strains, none were positive. PCR-restriction fragment Length polymorphism (PCR-RFLP) with HpaII yielded three types of patterns. PCR-RFLP 1 contained two fragments (66 and 166 bp) found in HG6, HG7, HCS, HG10, and HG11. PCR-RFLP 2 contained three fragments (18, 66, and 148 bp) found in HG1, HG2, HG3, and HG11. PCR-RFLP 3, with four fragments (7, 20, 66, and 139 bp), was observed only in HG13, PCR-amplicon sequence analysis (PCR-ASA) revealed three main types. PCR-ASA 1 had 76 to 78% homology with AHCYTOEN and included strains in HG6, HG7, HG8, HG10, and HG11. PCR-ASA 2, with 82% homology, was found only in HG13. PCR-ASA 3, with 91 to 99% homology, contained the strains in HG1, HG2, HG3, and HG11. This method indicated that 37 (61%) of the 61 reference strains were positive with the primer cocktail master mixture, and 34 (58%) of 59 environmental isolates, 93 (66%) of 141 food isolates, and 100 (67%) of 150 clinical isolates from around the world carried a virulence factor when primers AHCF1 and AHCR1 were used. In conclusion, this PCR-based method is rapid, sensitive, and specific for the detection of virulence factors of Aeromonas spp. It overcomes the handicap of time-consuming biochemical and other DNA-based methods.

关键词：气单胞菌属/遗传学气单胞菌属/分离和提纯气单胞菌属/致病力细菌毒素类/遗传学 DNA引物脱氧核糖核酸酶HpaⅡ 欧洲溶血素蛋白质类/遗传学肉/微生物学分子序列数据聚合酶链反应/方法多态性限制性片段长度成孔毒素蛋白质类家禽海味/微生物学猪蔬菜/微生物学毒力/遗传学动物牛

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RECURRENT AEROMONAS SEPSIS IN A PATIENT WITH LEUKEMIA

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AMERICAN JOURNAL OF CLINICAL PATHOLOGY 1975年第4期64卷 525-530页

作者： TAPPER, ML MCCARTHY, LR MAYO, JB ARMSTRONG, D CORNELL UNIV MED COLLNEW YORKNY MEM SLOAN KETTERING CANC CTR DEPT MEDINFECT DIS SERV1275 YORK AVENEW YORKNY 10021

A case of recurrent sepsis due to Aeromonas hydrophila in a patient with acute myelogenous leukemia is reported. The patient's first infection leading to bacteremia followed contamination of a mosquito bite by stagnant water. After recovery from the first bacteremia, the patient again became septic with a second strain of Aeromonas hydrophila, which again responded to antimicrobial therapy. It is hypothesized that contamination of the local water supply may have led to the establishment of a gastrointestinal carrier state that produced the second bout of Aeromonas sepsis when the patient was markedly leukopenic. The importance of the oxidase test to differentiate Aeromonas species from members of the family Enterobacteriaceae is re-emphasized.

关键词：气单胞菌属/分离和提纯氯霉素/治疗应用肠杆菌科感染/并发症肠杆菌科感染/药物疗法肠杆菌科感染/病因学庆大霉素类/治疗应用白血病髓样/并发症白血病髓样/微生物学复发成年人人类男(雄)性

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Incidence and identification of mesophilic Aeromonas spp. from retail foods

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LETTERS IN APPLIED MICROBIOLOGY 2000年第5期31卷 359-363页

作者： Neyts, K Huys, G Uyttendaele, M Swings, J Debevere, J State Univ Ghent Lab Food Microbiol & Food Preservat B-9000 Ghent Belgium State Univ Ghent Microbiol Lab Ghent Belgium

Sixty-eight food samples were examined for the presence of mesophilic Aeromonas species both qualitatively and quantitatively. Aeromonads were isolated from 26% of the vegetable samples, 70% of the meat and poultry samples and 72% of the fish and shrimps. Numbers of motile aeromonads present in the food samples varied from < 10(2) cfu g(-1) to > 10(5) cfu g(-1). GLC analysis of FAMEs was used to identify a selection of presumptive Aeromonas colonies to fenospecies or genomic species level. Aeromonas strains belonging to the Aer. caviae complex, which also includes the potentially pathogenic genospecies HG4, were mostly isolated from vegetables but were also found in meat, poultry and fish. In addition, three strains of the virulent taxon Aer. veronii biovar sobria HG8 were isolated from poultry and minced meat. All members of the Aer. hydrophila complex, predominant in the fish, meat and poultry samples, were classified in the non-virulent taxon HG3. Although the significance of Aeromonas in foods remains undefined, the isolation of Aeromonas HG4 and HG8 strains from a variety of retail foods may indicate that these products can act as possible vehicles for the dessimination of food-borne Aeromonas gastroenteritis.

关键词：气单胞菌属/分离和提纯细菌分型技术食品微生物学肉/微生物学家禽/微生物学海味/微生物学温度蔬菜/微生物学动物

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ACUTE ARTHRITIS CAUSED BY AEROMONAS-HYDROPHILA - CLINICAL AND THERAPEUTIC ASPECTS

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ARTHRITIS AND RHEUMATISM 1976年第2期19卷 169-172页

作者： CHMEL, H ARMSTRONG, D MEM SLOAN KETTERING CANC CTR DEPT MED SERV INFECT DIS SERV NEW YORK NY USA CORNELL UNIV COLL MED NEW YORK NY USA

Two cases of A. hydrophila arthritis are reported and a 3rd case present in the literature is reviewed. All 3 cases occurred in [immunoincompetent] patients with myelogenous leukemia;the knee joint was involved in 2 patients and the metacarpal-phalangeal joint in the 3rd. In the 2 patients presented here synovial fluid antibiotic levels were measured and were adequate;but A. hydrophila was repeatedly cultured from the knee joints, suggesting that other factors, i.e., normal functioning leukocytes and specific opsonizing antibody against the organism, are also necessary to kill A. hydrophila.

关键词：急性病气单胞菌属/分离和提纯气单胞菌属/致病力关节炎感染性/病因学关节炎感染性/微生物学肠杆菌科感染关节疾病/微生物学白血病髓样/并发症白血病髓样急性/并发症成年人女(雌)性人类男(雄)性

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Identification of inadequately cleaned equipment used in a sheep carcass-breaking process

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JOURNAL OF FOOD PROTECTION 1999年第6期62卷 637-643页

作者： Gill, CO Baker, LP Jones, T Agr & Agri Food Canada Res Ctr Lacombe AB T4L 1W1 Canada

Aeromonads deposited on pork during a carcass-breaking process were recovered on hydrophobic grid membrane filters placed on ampicillin-dextrin-ethanol agar. Isolates from 85 honey-yellow colonies from filters on that medium were Aeromonas hydrophila (95%) or Vibrio sp. (5%). Presumptive aeromonads, coliforms, and Escherichia coli in swab samples from product passing through a sheep carcass-breaking process were enumerated by hydrophobic grid membrane filtration techniques with a detection level of 1 CFU/100 cm(2). Total aerobic counts were determined by a spread plate procedure with a detection level of 1 CFU/cm(2) The numbers of aerobes, coliforms, and E. coli in the product were apparently unaffected by the carcass-breaking process, although coliforms and E. coli appeared to be redistributed from shoulder to loin and leg portions. However, the numbers of aeromonads on product increased by about two orders of magnitude as a result of the process. Few bacteria were recovered from most cleaned, large items of equipment, but aerobes, coliforms, and aeromonads were recovered at log total numbers of 5.25, 3.96, and 3.26, respectively, from most of 25 samples from bars supporting a conveyor belt. Also, aerobes, coliforms, E. coli, and aeromonads were recovered from 25 supposedly cleaned steel mesh gloves at log total numbers of 10.14, 5.54, 4.73, and 8.30, respectively Those findings indicate that inspection of cleaned equipment and microbiological sampling of only food-contacting surfaces, as is the current practice at meat plants, cannot provide assurance that the cleaning of carcass-breaking equipment is adequate. Instead, enumeration of indicator organisms on product passing through a process seems to be required as well, with subsequent sampling of equipment to identify sources of contaminants if increases in numbers during processing are observed. For surety of adequate cleaning, enumeration of several types of indicator organism may be necessary, as increases dur

关键词：气单胞菌属/分离和提纯细菌需氧/分离和提纯集落计数微生物肠杆菌科/分离和提纯设备污染肉/微生物学肉类加工业/方法肉类加工业/标准绵羊/微生物学猪/微生物学动物

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豚鼠气单胞菌被错误地鉴定为河流弧菌1例分析

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中国误诊学杂志 2008年第16期8卷 3894-3894页

作者：隋秀丽王燕宫浩山东省文登整骨医院 264400

2007-11我们从1例患者痰液标本中分离到一株发酵葡萄糖且氧化酶阳性的菌株,用商品化鉴定试剂盒鉴定,结果被错误地鉴定为河流弧菌。现报道如下。[第一段]

关键词：痰/微生物学气单胞菌属/分离和提纯弧菌属/分离和提纯

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