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现代医药卫生 2017年第14期33卷 2081-2083页

作者：王德婧胡斌丽严文霞徐卫红许洁遵义医学院附属医院贵州遵义563000 遵义医学院公共卫生学院贵州遵义563099

目的应用PCR芯片技术检测壬基酚(NP)暴露对仔鼠海马组织差异基因的表达,探讨NP神经毒性的机制。方法将交配成功的孕鼠根据妊娠日期分层后随机分配两组,即对照组(溶剂花生油)和NP暴露组[NP 200 mg/(kg·d)],每组7只,NP暴露时间为受孕... 详细信息

目的应用PCR芯片技术检测壬基酚(NP)暴露对仔鼠海马组织差异基因的表达,探讨NP神经毒性的机制。方法将交配成功的孕鼠根据妊娠日期分层后随机分配两组,即对照组(溶剂花生油)和NP暴露组[NP 200 mg/(kg·d)],每组7只,NP暴露时间为受孕第6天至出生后21 d,提取NP暴露组和对照组出生21 d仔鼠海马组织m RNA,应用大鼠神经毒理PCR芯片有针对性地分析暴露NP仔鼠模型海马组织的差异表达基因。结果与对照组相比,NP暴露组共有7个差异表达基因,上调超过2倍的基因有2个:Gal、Tph1;下调超过2倍的基因有5个:Camk2d、Casp7、Gch1、Trpm1、Tyrp1。其中,与凋亡相关的基因为Casp7、Gch1;代谢相关基因为Gal、Tph1、Tyrp1;离子转运相关基因为Camk2d、Trpm1。结论 NP能影响仔鼠神经系统的发育与功能,可能与神经元物质代谢、细胞凋亡、离子转运等相关基因表达改变密切相关。

关键词：海马聚合酶链反应生物传感技术/仪器和设备酚类/药理学仔鼠

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使生物传感器小型化的流体学立方体装置

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生命科学仪器 2003年第2期1卷 52页

作者：邵和玉

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Theoretical understanding of an absorption-based surface plasmon resonance sensor based on Kretchmann's theory

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ANALYTICAL CHEMISTRY 2002年第3期74卷 696-701页

作者： Kurihara, K Suzuki, K Kanagawa Acad Sci & Technol Takatsu Ku Kawasaki Kanagawa 2130012 Japan Keio Univ Dept Appl Chem Kohoku Ku Yokohama Kanagawa 2238522 Japan

An optical-absorption-based surface plasmon resonance (SPR) sensor is proposed, and its theoretical aspects are discussed in terms of mathematical descriptions and numerical simulations of the SPR curve. The response theory of the absorption-based SPR sensing is based on the expansion of Kretchmann's SPR theory into the case in which optical absorption in the sensing layer is expressed by the Lorentz model. The numerical simulations were performed using a three-layer Fresnel equation of p-polarization. It was found that SPR curve behavior of the absorption-based SPR sensor depends on the frequency relation between the light source and the optical absorption and the thickness of the metal layer. The SPR curve behavior is divided into three types according to the large, small, and equal relations between excitation and absorption frequencies. Each type of behavior is further divided into two types that are due to thin and thick metal layers. The theory of this new type of sensor based on optical absorption was explained and demonstrated by the simulation of the SPR curves using optical parameters relating to a silver-metal-based SPR sensor.

关键词：生物传感技术/仪器和设备生物传感技术/方法离子/分析模型理论散射辐射表面等离激元

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A three-dimensional numerical fluid dynamic model of antigen-antibody surface adsorption on piezoelectric immunosensors

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ANNALS OF BIOMEDICAL ENGINEERING 2002年第10期30卷 1313-1322页

作者： Barak-Shinar, D Rosenfeld, M Zisman, E Abboud, S Tel Aviv Univ Fac Engn Dept Biomed Engn IL-69978 Tel Aviv Israel Tel Aviv Univ Fac Engn Dept Fluid Mech & Heat Transfer IL-69978 Tel Aviv Israel Savyon Diagnost Ltd Ashdod Israel

A piezoelectric crystal is a unit that changes its frequency in parallel with a change in its mass. This characteristic is exploited in designing flow cell-based immunosensors for detecting the concentration of antibodies in liquid samples. In the present study, computational fluid dynamic techniques are used to optimize the antigen-antibody binding process on an electrode surface placed on the base of a conical flow cell. The geometry optimization of the flow cell was determined to minimize the test time. This time is needed for the electrode to be saturated by the antibody, a process that requires the maximization of the adsorption rate and be accomplished by increasing the shear rate in the vicinity of the electrode. To validate the numerical model and to determine its parameters, experiments were carried out using an identical flow cell. In the experiments, the system did not reach saturation within an acceptable time frame, therefore, the model parameters were determined based on the unsaturated state. The experimental results confirmed the applicability of numerical simulations in predicting the effect of changing the inlet section area of the flow cells, proving the computational model to be very valuable in designing immunosensors based on flow cells. (C) 2002 Biomedical Engineering Society.

关键词：吸附抗体/分析抗体/化学抗体细菌/分析抗体细菌/免疫学抗体单克隆/分析抗体单克隆/免疫学抗原抗体复合物/分析抗原抗体复合物/化学生物传感技术/仪器和设备计算机模拟计算机辅助设计结晶电化学/仪器和设备电化学/方法电极设备设计/方法大肠杆菌/免疫学流式细胞术/仪器和设备流式细胞术/方法免疫测定/仪器和设备免疫测定/方法模型生物学模型化学

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Simultaneous determination of pesticides using a four-band disposable optical capillary immunosensor

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ANALYTICAL CHEMISTRY 2002年第23期74卷 6064-6072页

作者： Mastichiadis, C Kakabakos, SE Christofidis, I Koupparis, MA Willetts, C Misiakos, K Natl Ctr Sci Res Demokritos Inst Radioiostopes & Radiodiagnost Prod Immunoassay Lab GR-15310 Athens Greece Natl Ctr Sci Res Demokritos Inst Microelect GR-15310 Athens Greece Univ Athens Dept Chem Analyt Chem Lab Athens 15771 Greece Syngenta Jealotts Hill Int Res Ctr Bracknell RG42 6EY Berks England

The development of a four-band capillary optical immunosensor for the simultaneous determination of mesotrione, hexaconazole, paraquat, and diquat is described. Four distinct bands (each corresponding to a different analyte) are created in the internal walls of a plastic capillary by immobilizing protein conjugates of the analytes. To perform the assay, the capillary is filled with a mixture of anti-analyte-specific antibodies together with a standard or sample containing the analyte(s). After a short incubation, a mixture of the appropriate second antibodies labeled with fluorescein is introduced into the capillary. To measure the fluorescence intensity bound onto each band, the capillary was scanned, perpendicularly to its axis, by a laser light beam. Part of the emitted photons were trapped into the capillary walls and waveguided to a photomultiplier placed at the one end of the capillary. The analytical characteristics of the assays of mesotrione, paraquat, diquat, and hexaconazole were as follows: detection limits of 0.04, 0.06, 0.09, and 0.10 ng/mL, respectively;dynamic ranges up to 9, 6, 12, and 15 ng/ ml, respectively;intra- and interassay CVs less than 10%. The analytical characteristics of the assays were comparable with those of the corresponding single-analyte fluoroimmunoassays performed in microtitration wells, proving the ability of the proposed immunosensor for reliable multianalyte determinations. Moreover, the combination of low-cost disposable plastic capillary tubes with the low consumption of reagents, the short assay time, and the multianalyte feature of the proposed immunosensor indicates its potential for environmental analysis.

关键词：生物传感技术/仪器和设备生物传感技术/方法生物传感技术/标准环己酮类/分析敌草快/分析一次性设备荧光素荧光染料免疫测定/仪器和设备免疫测定/方法免疫测定/标准百草枯/分析农药/分析敏感性与特异性三唑类/分析

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Sensor arrays and array sensors

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ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2002年第1期372卷 16-17页

作者： Wittstock, G Univ Oldenburg Dept Chem FB9 D-26111 Oldenburg Germany Univ Oldenburg ICBM D-26111 Oldenburg Germany

Electrochemical sensors are based either on the measurement of potentiometric, impedimetric, capacitive, amperometric, or voltammetric response of a specifically designed electrode. Increased mass transport towards the transducer and reduced Ohmic drop may improve the performance of sensors fabricated with one dimension smaller than the diffusion length of the analyte. The use of independent addressable sensors to construct multianalyte sensors and interdigitation of two comb-shaped arrangements constitute an important strategy for using ultramicroelectrode in modern electroanalytical chemistry. It is stated that arrays of individually addressable electrodes can be used to determine different analytes or to provide spatially resolved measurements.

关键词：生物传感技术/仪器和设备生物传感技术/方法酶类/分析酶类/代谢微电极显微镜检查电子扫描/仪器和设备显微镜检查电子扫描/方法氧化还原

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A disposable optrode using immobilized tyrosinase films

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ANALYTICAL BIOCHEMISTRY 2001年第2期294卷 102-107页

作者： Paranjpe, P Dutta, S Karve, M Padhye, S Narayanaswamy, R Univ Poona Dept Chem Poona 411007 Maharashtra India UMIST Dept Instrumentat & Analyt Sci Manchester M60 1QD Lancs England

A disposable fiber-optic sensor based on the immobilized tyrosinase enzyme in a composite biopolymer and its application for the detection of 3,4-dihydroxyphenyalanine (L-dopa) and its analog are described. The enzymatic oxidation product of L-dopa was stabilized through formation of an adduct with 3-methyl-2-benzothiazoline hydrazone resulting in enhanced accuracy and sensitivity of the measurements. The response was found to be linear and concentration dependent in the range of 5 x 10(-5) to 4 x 10(-4) M (r(2) = 0.9307) for the substrate L-dopa over the pH range 5.8 to 7.2 with response times of 8 min. The immobilized enzyme films are stable for 4 months when stored under moisture-free conditions at 4 degreesC. (C) 2001 Academic Press.

关键词：生物聚合物/化学生物传感技术/仪器和设备酶稳定性酶类固相/化学酶类固相/代谢纤维光学/仪器和设备氢离子浓度左旋多巴/化学左旋多巴/代谢分子结构一元酚单氧酶/化学一元酚单氧酶/代谢植物蛋白质类/化学植物蛋白质类/代谢光谱分析/方法

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A protein biosensor for lactate

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ANALYTICAL BIOCHEMISTRY 2000年第1期283卷 83-88页

作者： D'Auria, S Gryczynski, Z Gryczynski, I Rossi, M Lakowicz, JR Univ Maryland Ctr Fluorescence Spect Dept Biochem & Mol Biol Baltimore MD 21201 USA CNR Inst Prot Biochem & Enzymol I-80125 Naples Italy

Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactatesensing protein. (C) 2000 Academic Press.

关键词：苯胺基萘磺酸类/代谢生物传感技术/仪器和设备生物传感技术/方法剂量效应关系药物荧光染料/代谢同工酶类 L-乳酸脱氢酶/代谢乳酸/血液模型统计学心肌/酶学光谱法荧光动物牛

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Relative merits of optical biosensors based on flow-cell and cuvette designs

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ANALYTICAL BIOCHEMISTRY 2000年第2期285卷 179-193页

作者： Ward, LD Winzor, DJ Univ Queensland Dept Biochem Brisbane Qld 4072 Australia Virax Holdings Ltd Kew Vic 3101 Australia

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Direct measurement of adenosine release during hypoxia in the CA1 region of the rat hippocampal slice

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JOURNAL OF PHYSIOLOGY-LONDON 2000年第1期526卷 143-155页

作者： Dale, N Pearson, T Frenguelli, BG Univ Dundee Ninewells Hosp & Med Sch Dept Pharmacol & Neurosci Dundee DD1 9SY Scotland Univ Warwick Dept Sci Biol Coventry CV4 7AL W Midlands England

1.. We have used an enzyme-based, twin-barrelled sensor -to measure adenosine release during hypoxia in the CA1 region of rat hippocampal slices in conjunction with simultaneous extracellular field recordings of excitatory synaptic transmission. 2. When loaded with a combination of adenosine deaminase, nucleoside phosphorylase and xanthine oxidase, the sensor responded linearly to exogenous adenosine over the concentration range 10 nM to 20 mu M. 3. Without enzymes, the sensor when placed on the surface of hippocampal slices recorded a very small net signal during hypoxia of 40 +/- 43 pA (mean +/- S.E.M.;n = 7). Only when one barrel was loaded with the complete sequence of enzymes and the other with the last two in the cascade did the sensor record a large net difference signal during hypoxia (1226 +/- 423 pA;n = 7). 4. This signal increased progressively during the hypoxic episode, scaled with the hypoxic depression of the simultaneously recorded field excitatory postsynaptic potential and was greatly reduced (67 +/- 6.5%;n = 9) by coformycin (0.5-2 mu M), a selective inhibitor of adenosine deaminase, the first enzyme in the enzymic cascade within the sensor. 5. For 5 min hypoxic episodes, the sensor recorded a peak concentration of adenosine of 5.6 +/- 1.2 mu M (n = 16) with an IC(50) for the depression of transmission of approximately 3 mu M. 6. In slices pre-incubated for 3-6 h in nominally Ca(2+)-free artificial cerebrospinal fluid, 5 min of hypoxia resulted in an approximately 9-fold greater release of adenosine (48.9 +/- 17.7 mu M;n = 6). 7. High extracellular Ca(2+) (4 mM) both reduced the adenosine signal recorded by the sensor during hypoxia (3.5 +/- 0.6 mu M;n = 4) and delayed the hypoxic depression of excitatory synaptic transmission.

关键词：腺苷/代谢腺苷/药理学腺苷脱氨酶/代谢腺苷脱氨酶抑制剂生物传感技术/仪器和设备生物传感技术/方法钙/代谢钙/药理学细胞低氧/药物作用细胞低氧/生理学助间型霉素/药理学酶抑制剂/药理学评价研究(主题) 兴奋性突触后电位/药物作用兴奋性突触后电位/生理学海马/细胞学海马/药物作用海马/代谢缺氧脑/代谢戊糖基转移酶类/代谢大鼠 Sprague-Dawley 结果可重复性敏感性与特异性突触传递/药物作用突触传递/生理学黄嘌呤氧化酶/代谢动物女(雌)性男(雄)性大鼠

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