检索结果-内蒙古大学图书馆

Modeling of the interaction between new ethidium derivatives and TAR RNA of HIV-1

JOURNAL OF CHEMICAL INFORMATION AND COMPUTER SCIENCES 1999年第2期39卷 413-419页

作者： Terreux, R Cabrol-Bass, D Peytou, V Condom, R Guedj, R Univ Nice Equipe LARTIC GRECFO F-06108 Nice 2 France Univ Nice CNRS ESA 6001 Chim Bioorgan Lab F-06108 Nice France

During the HIV-1 replication process, interactions between the first sequence of RNA synthesized named TAR RNA and a viral protein named Tat permit a fast and efficient transcription of viral DNA in RNA. Based on the NMR structure of TAR RNA found on the PDB, new derivatives of ethidium were designed by molecular modeling to inhibit this interaction. The studied molecules are composed of three domains: an arginine, a linker, and an ethidium. Three linkers of different lengths were considered in the first step, with the TAR RNA-arginine interaction and the intercalation of the ethidium simulated by docking methods. In a second step, the structure of the TAR RNA was completed to obtain a whole ethidium interaction site and docking of the whole studied molecules was investigated. Molecules were synthesized and tested on infected cells. The predicted models and activity are in good agreement with the reported experimental results.

关键词：碱基序列结合部位药物设计胡米胺/类似物和衍生物胡米胺/化学胡米胺/药理学 HIV-1/药物作用 HIV-1/遗传学 HIV-1/生理学模型生物学模型分子核酸构象 RNA 病毒/化学 RNA 病毒/药物作用 RNA 病毒/遗传学 RNA结合蛋白质类/遗传学软件热力学病毒复制/药物作用人类

来源：评论

学校读者我要写书评

暂无评论

Inhibitors of strand transfer that prevent integration and inhibit HIV-1 replication in cells

引用

SCIENCE 2000年第5453期287卷 646-650页

作者： Hazuda, DJ Felock, P Witmer, M Wolfe, A Stillmock, K Grobler, JA Espeseth, A Gabryelski, L Schleif, W Blau, C Miller, MD Merck Sharp & Dohme Res Labs Dept Antiviral Res W Point PA 19486 USA

Integrase is essential for human immunodeficiency virus-type 1 (HIV-1) replication;however, potent inhibition of the isolated enzyme in biochemical assays has not readily translated into antiviral activity in a manner consistent with inhibition of integration. In this report, we describe diketo acid inhibitors of HIV-1 integrase that manifest antiviral activity as a consequence of their effect on integration. The antiviral activity of these compounds is due exclusively to inhibition of one of the two catalytic functions of integrase, strand transfer.

关键词：乙酰乙酸盐类/化学乙酰乙酸盐类/代谢乙酰乙酸盐类/药理学抗HIV药/化学抗HIV药/代谢抗HIV药/药理学催化作用/药物作用共同培养技术 DNA 环状/生物合成 DNA 环状/代谢 DNA 病毒/生物合成 DNA 病毒/代谢抗药性微生物 HIV整合酶/遗传学 HIV整合酶/代谢 HIV整合酶抑制剂/化学 HIV整合酶抑制剂/代谢 HIV整合酶抑制剂/药理学 HIV长末端重复序列/药物作用 HIV-1/药物作用 HIV-1/酶学 HIV-1/遗传学 HIV-1/生理学突变吡咯类/化学吡咯类/代谢吡咯类/药理学重组蛋白质类/拮抗剂和抑制剂重组蛋白质类/代谢 T淋巴细胞/病毒学转录遗传肿瘤细胞培养的病毒整合/药物作用病毒复制/药物作用人类

来源：评论

学校读者我要写书评

暂无评论

EFFICACY OF RIBAVIRIN AGAINST INFLUENZA-VIRUS IN TISSUE-CULTURE AND IN MICE

引用

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1975年第AUG8期255卷 366-371页

作者： DURR, FE LINDH, HF LEDERLE LABS PEARL RIVER NY 10965 USA

来源：评论

学校读者我要写书评

暂无评论

INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) TYPE-1 MULTIPLICATION BY AN AVIAN CELLULAR FACTOR

引用

ARCHIVES OF VIROLOGY 1992年第3-4期123卷 309-321页

作者： SEMMEL, M ALILECHE, A COULAUD, D AGUILAR, J KRIEF, P JASMIN, C INST RECH SCI CANC F-94802 VILLEJUIFFRANCE INST GUSTAVE ROUSSY F-94805 VILLEJUIFFRANCE

A factor secreted from avian cells infected non productively with a non cytopathogenic mutant of vesicular stomatitis virus (VSV ts 1026) interferes with HIV replication in CEM cells and peripheral blood monocytes (PBL). Production of infectious particles is decreased and many virions lack cores and/or spikes. In CEM cells the prmRNA is spliced into 7.5, 4, and 2 kb mRNA. Residual virus contains less env encoded proteins and p 18;p 25 appears as several bands. The processing of tat, rev, and nef proteins differs in treated cells and in controls.

关键词：印迹法 RNA 印迹法蛋白质细胞系细胞因子类/代谢细胞因子类/药理学成纤维细胞/代谢成纤维细胞/微生物学 HIV-1/药物作用 HIV-1/生理学 HIV-1/超微结构淋巴细胞活化显微镜检查电子 RNA 信使/生物合成 RNA 信使/药物作用 RNA 病毒/生物合成 RNA 病毒/药物作用 T淋巴细胞/免疫学 T淋巴细胞/微生物学转录遗传水疱性口炎印第安纳病毒/生理学病毒复制/药物作用动物鸡胚

来源：评论

学校读者我要写书评

暂无评论

Potentiation of the anti-HIV activity of zalcitabine and lamivudine by a CTP synthase inhibitor, 3-deazauridine

引用

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS 2000年第1-2期19卷 371-377页

作者： Gao, WY Johns, DG Mitsuya, H NCI Med Chem Lab Div Basic Sci NIH Bethesda MD 20892 USA NCI Expt Retrovirol Sect Med Branch Div Clin SciNIH Bethesda MD 20892 USA

Low levels of the CTP synthase inhibitor 3-deazauridine (3-DU) strongly potentiated the anti-HLV-1 activity of the 5'-triphosphates of the cytidine-based analogues [-]2'-deoxy-3'-thiacytidine (3TC;lamivudine) and 2',3'-dideoxycytidine (ddC). The potentiation was associated with a 3-DU-induced decrease in dCTP pool size;no changes were seen in cellular pool sizes of dATP, dGTP or dTTP.

关键词： 3-脱氧尿苷/药理学抗HIV药/药理学碳氮连接酶类/拮抗剂和抑制剂脱氧胞苷激酶/代谢药物协同作用酶抑制剂/药理学 HIV-1/药物作用拉米夫定/药理学白细胞单核/药物作用白细胞单核/病毒学逆转录酶抑制剂/药理学病毒复制/药物作用扎昔他宾/药理学人类

来源：评论

学校读者我要写书评

暂无评论

Inhibition of various steps in the replication cycle of vesicular stomatitis virus contributes to its photoinactivation by AlPcS₄ or Pc4 and red light

引用

PHOTOCHEMISTRY AND PHOTOBIOLOGY 1999年第3期69卷 353-359页

作者： Moor, ACE Wagenaars-van Gompel, AE Hermanns, RCA van der Meulen, J Smit, J Wilschut, J Brand, A Dubbelman, TMAR VanSteveninck, J Leiden Univ Med Ctr Immunohematol & Blood Bank NL-2300 RA Leiden Netherlands Leiden Univ Med Ctr Ctr Electron Microscopy NL-2300 RA Leiden Netherlands Univ Groningen Groningen Netherlands Leiden Amsterdam Ctr Drug Res Leiden Netherlands

Vesicular stomatitis virus (VSV) was used as a model virus to study the processes involved in photoinactivation by aluminum phthalocyanine tetrasulfonate (AIPcS(4),) or silicon phthalocyanine HOSiPcOSi(CH3)(2)(CH2)(3)N(CH3)(2) (Pc4) and red light. Previously a very rapid decrease in the intracellular viral RNA synthesis after photodynamic treatment was observed. This decrease was correlated to different steps in the replication cycle. Binding of VSV to host cells and internalization were only slightly impaired and could be visualized by electron microscopy. The capability of the virus to fuse with membranes in an acidic endosomal environment was studied using both pyrene-labeled liposomes and a hemolysis assay as a model. These tests indicate a rapid decrease of fusion capacity after AIPcS(4), treatment, which correlated with the decrease in RNA synthesis, For Pc4 treatment no such correlation was found. The fusion process is the first step in the replication cycle, affected by AIPcS(4) treatment, but also in vitro RNA polymerase activity was previously shown to be inhibited. Inactivation of VSV by Pc4 treatment is apparently caused by damage to a variety of viral components. Photodynamic treatment of virus suspensions with both sensitizers causes formation of 8-oxo-7,8-dihydroguanosine in viral RNA as measured by HPLC with electrochemical detection. This damage might be partly responsible for inhibition of the in vitro viral RNA polymerase activity by photodynamic treatment.

关键词：细胞系仓鼠亚科吲哚类/药理学光显微镜检查电子有机金属化合物/药理学有机硅化合物/药理学光化学疗法光敏感药/药理学 RNA/药物作用 RNA/辐射效应硅烷类水疱性口炎印第安纳病毒/药物作用水疱性口炎印第安纳病毒/生理学水疱性口炎印第安纳病毒/辐射效应病毒复制/药物作用病毒复制/辐射效应动物

来源：评论

学校读者我要写书评

暂无评论

F FACTOR-MEDIATED INHIBITION OF BACTERIOPHAGE T7 GROWTH - ANALYSIS OF T7 RNA AND PROTEIN-SYNTHESIS INVIVO AND INVITRO USING MALE AND FEMALE ESCHERICHIA-COLI

引用

JOURNAL OF MOLECULAR BIOLOGY 1975年第1期98卷 31-43页

作者： CONDIT, RC STEITZ, JA YALE UNIV DEPT MOLEC BIOPHYS & BIOCHEM NEW HAVEN CT 06510 USA

We have used two approaches to determine whether the inhibition of phage T7 growth by the F factor in male Escherichia coli can be ascribed to a preferential inhibition of late phage protein synthesis. (1) Proteins and RNAs were pulselabeled with [ 35 S]methionine and 32 PO 4 2− during T7 infection of male and female E. coli , and the products were analyzed by polyacrylamide gel electrophoresis. Compared to T7 infected female E. coli , a simultaneous inhibition of protein and RNA synthesis is observed in male cells between eight and ten minutes after infection at 30°C. (2) Late T7 proteins were labeled with [ 35 S]methionine in a DNA-dependent coupled in vitro protein synthesizing system using extracts from uninfected and T7 infected male and female E. coli and analyzed by polyacrylamide gel electrophoresis. Under conditions where faithful synthesis of 16 out of 20 phage proteins is directed by T7 DNA in vitro , extracts derived from uninfected or T7-infected male E. coli are fully active in phage-specific transcription and translation. We therefore conclude that the cessation of RNA and protein synthesis in T7-infected male E. coli cannot be directly ascribed to any specific modification or inhibition of the transcription or translation apparatus.

关键词：细菌蛋白质类/药理学细菌蛋白质类/生理学大肠杆菌噬菌体/药物作用大肠杆菌噬菌体/代谢大肠杆菌/代谢大肠杆菌/生理学基因型质粒蛋白质生物合成/药物作用 RNA 病毒/生物合成种特异性转录遗传/药物作用病毒蛋白质类/生物合成病毒复制/药物作用

来源：评论

学校读者我要写书评

暂无评论

Genetic analysis reveals that both haemagglutinin and neuraminidase determine the sensitivity of naturally occurring avian influenza viruses to zanamivir in vitro

引用

VIROLOGY 1999年第2期263卷 323-338页

作者： Baigent, SJ Bethell, RC McCauley, JW Inst Anim Hlth Dept Mol Biol Newbury RG20 7NN Berks England Glaxo Wellcome Med Res Ctr Dept Enzyme Pharmacol Stevenage SG1 2NY Herts England

The basis of differential sensitivity of replication of influenza Viruses to the neuraminidase-specific inhibitor zanamivir was examined using four avian influenza viruses and reassortants produced between them. IC50 values for inhibition of neuraminidase activity by zanamivir were similar for each of the four viruses, whereas the haemagglutinating activity of each of the viruses was relatively insensitive to zanamivir. However, the four viruses showed distinct zanamivir-sensitivity profiles in tissue culture. Analysis of the reassortant viruses showed that sensitivity was determined by the haemagglutinin gene (segment 4) and the neuraminidase gene (segment 6) and was independent of the remaining six RNA segments. Decreased sensitivity to zanamivir was associated with possession of a haemagglutinin that is released from cells with decreased dependence on neuraminidase and with possession of a neuraminidase that has a short stalk region. (C) 1999 Academic Press.

关键词：氨基酸序列抗病毒药/药理学碱基序列细胞培养的鸡/血液鸡/病毒学红细胞/病毒学基因病毒/遗传学基因病毒/生理学糖基化胍类血凝集病毒性/药物作用血凝素类病毒/遗传学血凝素类病毒/代谢流感病毒A型/药物作用流感病毒A型/酶学流感病毒A型/遗传学流感病毒A型/生理学半数抑制浓度乳糖/类似物和衍生物乳糖/代谢分子序列数据突变/遗传学神经氨酸酶/拮抗剂和抑制剂神经氨酸酶/化学神经氨酸酶/遗传学神经氨酸酶/代谢吡喃类再装配病毒/药物作用再装配病毒/酶学再装配病毒/遗传学再装配病毒/生理学唾液酸类/代谢唾液酸类/药理学底物特异性病毒复制/药物作用扎那米韦动物鸡胚狗

来源：评论

学校读者我要写书评

暂无评论

Anti-HIV-1 activity of enfuvirtide (T-20) by inhibition of bystander cell death

引用

ANTIVIRAL THERAPY 2003年第2期8卷 155-161页

作者： Barretina, J Blanco, J Armand-Ugón, M Gutiérrez, A Clotet, B Esté, JA Univ Autonoma Barcelona Hosp Germans Trias & Pujol Retrovirol Lab IrsiCaixa Badalona 08916 Spain

Infection by human immunodeficiency virus type 1 (HIV-1) has been associated with increased cell death of both infected and bystander cells. The envelope glycoprotein complex appears to play an active role in HIV-induced death of bystander cells. We quantified cell-to-cell fusion, single cell death and membrane lipid mixing in cocultures of effector, HIV-1 envelope-expressing cells with peripheral blood mononuclear cells or purified CD4 T lymphocytes from HIV-negative donors, in the presence or the absence of the fusion inhibitor enfuvirtide (T-20, pentafuside, Fuzeon (TM)). T-20, which blocks gp41-dependent virus-cell fusion, showed a complete and dose-dependent inhibition of syncytium formation in cocultures of envelope-expressing cells with uninfected cells. Similarly, T-20 totally abrogated death of single bystander CD4 T cells with an IC50 of 0.04 mu g/ml. Membrane lipid mixing, as a measure of interaction between envelope-expressing cells and CD4 cells, was also dose-dependently inhibited by T-20. Moreover, effector cells chronically infected with a T-20-resistant virus recovered the ability to induce bystander cell death in the presence of the drug, supporting the role of gp41 in single cell death. In conclusion, T-20 is able to protect CD4 T cells from envelope presentation with a dual effect: inhibition of virus replication and blockade of HIV-1 envelope-induced cell death of bystander CD4 T cells. Protection of cells prior to infection from HIV envelope-dependent bystander effect could lead to a better immune restoration of HIV-1-infected patients that are treated with T-20.

关键词：抗HIV药/药理学 CD4阳性T淋巴细胞/药物作用 CD4阳性T淋巴细胞/病理学细胞死亡/药物作用细胞培养的共同培养技术剂量效应关系药物巨细胞/药物作用 HIV包膜蛋白质gp41/代谢 HIV包膜蛋白质gp41/药理学 HIV-1/药物作用 HIV-1/致病力肽碎片/药理学毒力/药物作用病毒复制/药物作用人类

来源：评论

学校读者我要写书评

暂无评论

Metabolism of O⁶-propyl and N⁶-propyl-carbovir in CEM cells

引用

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS 2000年第4期19卷 795-804页

作者： Parker, WB Shaddix, SC Rose, LM Pham, PT Hua, M Vince, R So Res Inst Birmingham AL 35205 USA Univ Minnesota Coll Pharm Dept Med Chem Minneapolis MN 55455 USA

The metabolism of O-6-propyl-carbovir and N-6-propyl-carbovir, two selective inhibitors of HIV replication, has been evaluated in CEM cells. Both compounds were phosphorylated in intact cells to carbovir-5'-triphosphate. The metabolism of these two agents was inhibited by deoxycoformycin and mycophenolic acid, but not erythro-P (2-hydroxy-3-nonyl)adenine. No evidence of the 5'-triphosphate of either compound was detected in CEM cells.

关键词：烷化作用抗HIV药/拮抗剂和抑制剂抗HIV药/化学合成抗HIV药/代谢细胞系/代谢双脱氧核苷类/拮抗剂和抑制剂双脱氧核苷类/化学合成双脱氧核苷类/代谢 HIV-1/药物作用 HIV-1/代谢霉酚酸/药理学喷司他丁/药理学磷酰化/药物作用病毒复制/药物作用

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：