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检索条件"主题词=病毒蛋白质类/分离和提纯"
20 条 记 录,以下是1-10 订阅
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Replication-associated activities of purified human papillomavirus type 11 E1 helicase
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PROTEIN EXPRESSION AND PURIFICATION 2000年 第2期18卷 148-159页
作者: Rocque, WJ Porter, DJT Barnes, JA Dixon, EP Lobe, DC Su, JL Willard, DH Gaillard, R Condreay, JP Clay, WC Hoffman, CR Overton, LK Pahel, G Kost, TA Phelps, WC Glaxo Wellcome Inc Dept Mol Sci Res Triangle Pk NC 27709 USA Glaxo Wellcome Inc Dept Mol Biochem Res Triangle Pk NC 27709 USA Glaxo Wellcome Inc Dept Virol Res Triangle Pk NC 27709 USA
Replication of human papillomavirus type11 (HPV11) requires both the E1 and the E2 proteins. E1 is structurally and functionally similar to SV40 large T-antigen and is a DNA helicase/NTPase that binds to the origin of... 详细信息
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Helix-destabilizing properties of the baculovirus single-stranded DNA-binding protein (LEF-3)
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VIROLOGY 2000年 第1期270卷 180-189页
作者: Mikhailov, VS NK Koltsov Dev Biol Inst Moscow 117808 Russia Inst Phys & Chem Res Lab Mol Entomol & Baculovirol Wako Saitama Japan
The helix-destabilizing properties of a single-stranded DNA-binding protein, LEF-3, of Bombyx mori nucleopolyhedrovirus (BmNPV) were studied. Partial duplexes of DNA containing single-stranded (ss) tails of different ... 详细信息
来源: 评论
Recombinant expression and modification analysis of protein agno-1b encoded by avian polyomavirus BFDV
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ARCHIVES OF VIROLOGY 2000年 第6期145卷 1211-1223页
作者: Liu, Q Hintz, M Li, J Linder, M Geyer, R Hobom, G Univ Giessen Inst Mikrobiol & Mol Biol D-35392 Giessen Germany Univ Giessen Klinikum Inst Biochem D-6300 Giessen Germany
Among two pairs of agnoproteins encoded in upstream positions in the late mRNAs of avian polyomavirus BFDV, either agno-1a or its splice derivative agno-1b are required for viral propagation. Out of the two proteins b... 详细信息
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Crystallization and preliminary X-ray analysis of the matrix protein from Ebola virus
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ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 2000年 第6期56卷 758-760页
作者: Dessen, A Forest, E Volchkov, V Dolnik, O Klenk, HD Weissenhorn, W European Mol Biol Lab F-38000 Grenoble France Inst Biol Struct Jean Pierre Ebel F-38027 Grenoble France Inst Virol D-35037 Marburg Germany
The matrix protein from Ebola virus is a membrane-associated molecule that plays a role in viral budding. Despite its functional similarity to other viral matrix proteins, it displays no sequence similarity and hence ... 详细信息
来源: 评论
Synthesis, subcellular localization and VP16 interaction of the herpes simplex virus type 2 UL46 gene product
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ARCHIVES OF VIROLOGY 2000年 第10期145卷 2149-2162页
作者: Kato, K Daikoku, T Goshima, F Kume, H Yamaki, K Nishiyama, Y Nagoya Univ Sch Med Dis Mechanism & Control Res Inst Nagoya Aichi 4668550 Japan Nagoya Univ Sch Med Dept Internal Med 2 Nagoya Aichi 466 Japan
We developed a rabbit polyclonal antiserum reactive against a recombinant 6x His-UL46 fusion protein expressed in Escherichia coli, and using this antiserum identified the UL46 gene product of herpes simplex virus typ... 详细信息
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Human cytomegalovirus - no longer just a DNA virus
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NATURE MEDICINE 2000年 第8期6卷 863-864页
作者: Huang, ES Johnson, RA Univ N Carolina Dept Med Chapel Hill NC 27599 USA Univ N Carolina Lineberger Comprehens Canc Ctr Chapel Hill NC 27599 USA
Human cytomegalovirus (HCMV) is classified as a DNA herpesvirus. A recent study showed that the HCMV virion contains not only DNA, but also four species of mRNA, indicating that this virus is more complex than previou... 详细信息
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Differential functional behavior of viral φ29, Nf and GA-1 SSB proteins
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NUCLEIC ACIDS RESEARCH 2000年 第10期28卷 2034-2042页
作者: Gascón, I Lázaro, JM Salas, M Univ Autonoma Madrid Ctr Biol Mol Severo Ochoa CSIC UAM E-28049 Madrid Spain
DNA replication of phi 29 and related phages takes place via a strand displacement mechanism, a process that generates large amounts of single-stranded DNA (ssDNA). Consequently, phage-encoded ssDNA-binding proteins (... 详细信息
来源: 评论
Viral coinfection in salmonids:: Infectious pancreatic necrosis virus interferes with infectious hematopoietic necrosis virus
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ARCHIVES OF VIROLOGY 1999年 第4期144卷 657-673页
作者: Alonso, M Rodríguez, S Pérez-Prieto, SI CSIC Ctr Invest Biol Dept Mol Microbiol E-28006 Madrid Spain
Coinfection of farm-reared salmonids involving two viruses has been described, but there is no report on the interactions between viruses. Here we examine whether infectious pancreatic necrosis virus (IPNV) strain Sp ... 详细信息
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Identification of MaTu-MX agent as a new strain of lymphocytic choriomeningitis virus (LCMV) and serological indication of horizontal spread of LCMV in human population
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VIROLOGY 1999年 第1期257卷 73-83页
作者: Reiserová, L Kaluzová, M Kaluz, S Willis, AC Závada, J Závodská, E Závadová, Z Ciampor, F Pastorek, J Pastoreková, S Slovak Acad Sci Inst Virol Bratislava 84246 Slovakia Univ Oxford Dept Biochem MRC Immunochem Unit Oxford OX1 3QU England Acad Sci Czech Republ Inst Mol Genet Prague Czech Republic
In this study we elucidated the molecular character of MaTu-MX, previously described as an unusual transmissible agent. Amino acid sequencing of peptides generated from a 58-kDa MX-related protein purified from MaTu h... 详细信息
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Histidine codons appended to the gene encoding the RP022 subunit of vaccinia virus RNA polymerase facilitate the isolation and purification of functional enzyme and associated proteins from virus-infected cells
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VIROLOGY 1999年 第2期258卷 469-479页
作者: Katsafanas, GC Moss, B NIAID Viral Dis Lab NIH Bethesda MD 20892 USA
Vaccinia virus encodes a eukaryotic-like RNA polymerase composed of two large and six small subunit protein species. A replication-competent virus with 10 histidine codons added to the single endogenous J4R open readi... 详细信息
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