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中华外科杂志 1982年第5期20卷 275-277页

作者：朱兆明王玉芝李元敏中国人民解放军总医院烧伤科军事医学科学院放射医学研究所

在低温条件下储存保持活力的生物组织,早已被广泛地应用于畜牧、生物及医学方面。我们于1973年开始进行了利用液态氮储存异体皮的研究工作,并已大量应用于临床,成功地抢救了很多大面积深度烧伤病人。但我们发现经低温储存的皮肤的琥珀... 详细信息

在低温条件下储存保持活力的生物组织,早已被广泛地应用于畜牧、生物及医学方面。我们于1973年开始进行了利用液态氮储存异体皮的研究工作,并已大量应用于临床,成功地抢救了很多大面积深度烧伤病人。但我们发现经低温储存的皮肤的琥珀酸脱氢酶的活力(改良的 Hershey 氏法)只为储存前的45～

关键词：冷冻外科手术皮肤/超微结构

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激光脱毛术后家狗皮肤组织学和超微结构变化

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浙江大学学报（医学版） 2003年第4期32卷 330-334,341页

作者：黄新炎郑敏任秋实浙江大学医学院附属第二医院浙江杭州310009 上海市静安区康奥医院整形美容激光中心

目的 :探讨翠绿宝石激光脱毛术后的皮肤组织学和超微结构的改变。方法 :对 7只黑毛狗用翠绿宝石激光脱毛 ,术后 0 .5 h,2、3、5、10及 30 d分别取材 ,在光镜和透射电镜下观察皮肤标本。结果 :激光术后 ,皮肤中毛干凝固及碳化 ,治疗区损... 详细信息

目的 :探讨翠绿宝石激光脱毛术后的皮肤组织学和超微结构的改变。方法 :对 7只黑毛狗用翠绿宝石激光脱毛 ,术后 0 .5 h,2、3、5、10及 30 d分别取材 ,在光镜和透射电镜下观察皮肤标本。结果 :激光术后 ,皮肤中毛干凝固及碳化 ,治疗区损伤的毛囊与未损伤的毛囊散乱分布 ,黑素细胞分布多的基底层可见微观改变。术后 10 d内可见轻微的炎性反应。能量大的治疗区毛囊损伤程度大 ,单脉冲及双脉冲激光术后毛囊损伤程度无明显差异。结论 :翠绿宝石激光对毛囊有选择性损伤作用 ,同时有表皮基底层的微观改变 ,术后无疤痕形成 ;翠绿宝石激光脱毛的疗效有能量依赖性 ;双脉冲与单脉冲激光脱毛疗效无明显差异 ;激光枪头备有冷却装置可减少术后的副反应。

关键词：激光/治疗应用毛发去除/方法皮肤/超微结构狗

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“围刺”对自然老化大鼠真皮羟脯氨酸含量及超微结构的影响

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中国针灸 2008年第1期28卷 61-64页

作者：芦源辽宁中医药大学附属医院医学美容科沈阳110032

目的:探讨针刺疗法抗皮肤老化的作用机制。方法:选用18月龄大鼠腹部皮肤为研究对象,采用毫针局部围刺作为处理因素,同时设立空白组、小切口除皱手术对照组、8月龄青年大鼠组,比较其皮肤可溶性及总羟脯氨酸含量,并通过透射电镜观察其成... 详细信息

目的:探讨针刺疗法抗皮肤老化的作用机制。方法:选用18月龄大鼠腹部皮肤为研究对象,采用毫针局部围刺作为处理因素,同时设立空白组、小切口除皱手术对照组、8月龄青年大鼠组,比较其皮肤可溶性及总羟脯氨酸含量,并通过透射电镜观察其成纤维细胞的形态以及胶原纤维的排列,研究局部围刺对老化皮肤胶原纤维含量及真皮超微结构的影响,并与参考文献方法进行手术的实验结果相对照。结果:围刺后大鼠皮肤可溶性羟脯氨酸含量显著高于老年空白组,总羟脯氨酸无显著差异。电镜可见,老龄大鼠真皮成纤维细胞的细胞器减少,细胞结构退化,同时胶原纤维之间空隙加宽,老化性架桥增多,围刺治疗后其成纤维细胞活性增强。结论:围刺法可能是通过增强皮肤中成纤维细胞的活性进而增加其可溶性胶原含量来改变皮肤的老化状态。

关键词：围刺皮肤/代谢羟脯氨酸/针灸效应皮肤/超微结构大鼠

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中国人使用800nm半导体激光脱毛的疗效和组织学研究

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四川大学学报（医学版） 2009年第2期40卷 361-363页

作者：陆华蒋献黄俊李利杜小平李咏邓次冰四川大学华西医院皮肤科成都610041 郧阳医学院附属人民医院整形美容科

目的观察中国人使用800nm半导体激光脱毛仪脱毛的临床疗效和组织病理变化，评价中国人使用激光脱毛的疗效和安全性。方法82例患者使用半导体激光脱毛仪治疗（包括腋窝、双下肢、面颊、上唇），每次治疗间隔6周，以脱毛率评价脱毛的疗效... 详细信息

目的观察中国人使用800nm半导体激光脱毛仪脱毛的临床疗效和组织病理变化，评价中国人使用激光脱毛的疗效和安全性。方法82例患者使用半导体激光脱毛仪治疗（包括腋窝、双下肢、面颊、上唇），每次治疗间隔6周，以脱毛率评价脱毛的疗效。取10名患者第1次治疗前、第1次治疗后30min、第2次治疗前、第3次治疗后3月的皮肤组织（腋窝），于光镜及透射电镜下观察脱毛前后皮肤的组织病理变化和超微结构改变。结果经过3次治疗后毛发较治疗前明显减少，不同部位脱毛率不同，腋窝3次治疗后的脱毛率达81％；上唇脱毛率最低，为50％左右。光镜观察可见治疗后破坏、退行的毛囊，表皮结构未见改变，胶原纤维可见肿胀但未见断裂。透射电镜观察可见治疗后黑素颗粒破碎和呈空泡状。4例患者治疗后出现色素沉着，均于术后1～6月内消退。结论中国人使用800nm半导体激光脱毛仪脱毛安全、有效。

关键词：半导体激光脱毛毛囊病理学皮肤/超微结构

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WIDE-FIELD ELECTRON-MICROSCOPY - RAPID METHOD FOR STUDY OF HISTOLOGIC MATERIAL THAT PROVIDES A BRIDGE BETWEEN LIGHT AND ELECTRON-MICROSCOPY

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AMERICAN JOURNAL OF CLINICAL PATHOLOGY 1975年第5期64卷 648-654页

作者： YANG, GCH MORRISON, AB COLL MED & DENT NEW JERSEY RUTGERS MED SCH DEPT PATHOL PISCATAWAY NJ 08854 USA

Wide fields of tissue can be rapidly examined by electron microscopy by use of Formvar films for the support of ultrathin sections on slot grids. The intervention of the grid bars of conventional mesh grids is avoided, and a continuous micrograph of the specimen at scanning magnifications can be obtained. Enough material is sublimed from the section and the supporting film by deliberate exposure to the electron beam to permit one to obtain an image with good contrast. This method of examination, which takes in all about two hours, permits examination of an extensive area of tissue in relation both to its topography at low magnifications and to its ultrastructural detail, and accordingly adds to electron microscopy a dimension characteristic of the lower powers of the light microscope. It offers the histopathologist the option of using micrographs taken during the scanning survey of a tissue to detect regions that can be readily re-examined at high magnification in the same ultrathin section.

关键词：肾/超微结构肾小球/超微结构显微镜检查电子/仪器和设备显微镜检查电子/方法皮肤/超微结构动物儿童学龄前女(雌)性人类大鼠

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CUTANEOUS INFECTION DUE TO A ROUGH VARIANT OF MYCOBACTERIUM-MARINUM

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AMERICAN JOURNAL OF CLINICAL PATHOLOGY 1975年第2期64卷 263-270页

作者： SMITH, AG JIJI, RM UNIV MARYLAND SCH MEDDEPT PATHOLBALTIMOREMD 21201

Spreading lesions clinically resembling lymphangitic sporotrichosis developed on the right arm and chest of a 60-year-old man with chronic lymphocytic leukemia. Acid-fast bacilli were seen in exudates from lesions and in biopsies, and were cultured from them. The isolant grew initially as a yellowish-orange scotochromogen on Lowenstein-Jensen medium at room temperature and at 35 C., but failed to grow at 37 C. It failed to grow on 7-H-10 medium. On repeated subculturing over a 2-year period it gradually converted to a photochromogen. Histologically, there was ulceration with extensive acute and chronic inflammation with fibrosis. Organisms occurred intracellularly as dense, compact, cigar-like packets resembling lepara bacilli. The appeared to have a predilection for the nucleus. The patient was anergic to PPD S, B, Y and G, and lacked antibodies to BCG phosphoglycolipids. The mycobacteriosis was alleviated by combined INH and ethambutol therapy. The isolant was identified as a rough variant of Mycobacterium marinum. It may have been transmitted by an insect vector.

关键词：抗体细菌抗原细菌卡介苗细菌学技术细胞核/微生物学培养基白血病淋巴样/并发症分枝杆菌属/生长和发育分枝杆菌属/免疫学分枝杆菌属/分离和提纯分枝杆菌属/代谢牛分枝杆菌/免疫学坏死色素类生物学/生物合成皮肤/超微结构温度结核皮肤/并发症结核皮肤/微生物学结核皮肤/病理学人类男(雄)性中年人

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Heterocyclic derivatives of all-trans retinoic acid:: in vitro effects on fibroblast/keratinocyte growth and differentiation, and in vivo effects on guinea-pig skin

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PHARMACOLOGY & TOXICOLOGY 1999年第2期85卷 49-55页

作者： Parnigotto, PP Conconi, MT Bortoletto, C Manfredini, S Montesi, F Schiavon, M Cancellotti, FM Univ Padua Interdepartmental Ctr Study Keratinocytes Pharmac I-35131 Padua Italy Univ Ferrara Dept Pharmaceut Sci I-44100 Ferrara Italy Expt Zooprophylact Inst Venezie I-35020 Padua Italy

We investigated the biological effects of Eve all-trans retinoic acid derivatives, bearing heterocyclic ring systems in the side chain. Growth assays performed on submerged human fibroblast and keratinocyte cultures revealed that (E)4-[2-(5-terbuthyl-thiophen-2-yl)propenyl]benzoic acid (compound 5) is the best compound among the studied derivatives for it exibits a weaker antiproliferative activity and induces, like all-trans retinoic acid does, a significant increase in fibroblast and keratinocyte growth. The morphological and morphometrical analyses of submerged human fibroblast cultures and human epidermis reconstructed in vitro shelved that the compound 5 behaves similarly to all-trans retinoic acid: it induces a decrease in all the cell parameters of submerged fibroblast cultures, and modulates the differentiation of keratinocytes in in vitro reconstructed epidermis. Compound 5 induces thickening of epidermis in vivo, one of the most remarkable pharmacological effects of retinoids on skin, but compared to all-trans retinoic acid, it induces a weaker irritation on guinea-pig skin in terms of both erythema and scaling. Compound 5 could then represent a promising candidate for the treatment of certain dermatological diseases.

关键词：细胞分化/药物作用着色剂成纤维细胞/药物作用成纤维细胞/超微结构荧光抗体技术杂环化合物/药理学异构现象角蛋白细胞/药物作用角蛋白细胞/超微结构角质溶解药/药理学皮肤/细胞学皮肤/药物作用皮肤/生长和发育皮肤/超微结构四唑鎓盐类噻唑类维甲酸/类似物和衍生物维甲酸/药理学动物儿童学龄前豚鼠人类婴儿

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THIAMINE PYROPHOSPHATASE AND ACID-PHOSPHATASE - THEIR ULTRASTRUCTURAL LOCALIZATION IN RAT SKIN

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ARCHIV FUR DERMATOLOGISCHE FORSCHUNG 1975年第2期252卷 131-137页

作者： ITOIZ, ME DEREY, BM CABRINI, RL UNIV BUENOS AIRES FAC DENTORAL PATHOL DEPTBUENOS AIRESARGENTINA COMISION NACL ENERGIA ATOM DEPT RADIOBIOLAVDA DEL LIBERATADOR 8250BUENOS AIRESARGENTINA

The ultrastructural localization of TPPase and AcPase in rat skin was studied.

关键词：酸性磷酸酶/分析细胞膜/酶学细胞质/酶学细胞外隙/酶学高尔基体/酶学组织细胞化学角蛋白质类/生物合成溶酶体/酶学焦磷酸酶类/分析皮肤/酶学皮肤/超微结构硫胺素焦磷酸盐动物大鼠

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Mechanical forces induce scar remodeling -: Study in non-pressure-treated versus pressure-treated hypertrophic scars

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AMERICAN JOURNAL OF PATHOLOGY 1999年第5期155卷 1671-1679页

作者： Costa, AMA Peyrol, S Pôrto, LC Comparin, JP Foyatier, JL Desmoulière, A Univ Bordeaux 2 INSERM GREF F-33076 Bordeaux France CNRS Inst Biol & Chim Prot Lyon France Univ Estado Rio de Janeiro Dept Histol & Embriol Rio De Janeiro Brazil Fac Med Laennec Serv Commun Microscopie Elect Lyon France Ctr Hosp St Joseph & St Luc Ctr Brules Lyon France

Reparative process of second and third degree burns usually results in hypertrophic scar formation that can be treated by pressure. Although this method is efficient, its mechanisms of action are not known. In this work, we have studied the histological organization of hypertrophic scars submitted to pressure. Skin biopsies were performed 2 to 7 months after the onset of treatment in two adjacent regions of the scar, non-pressure- or pressure-treated and analyzed by immunohistochemistry and transmission electron microscopy for extracellular matrix organization and cellular morphology, In non-pressure-treated regions, fibrillin deposits did not present the classical candelabra-like pattern under epidermis and were reduced in dermis;in pressure-treated regions the amount was increased compared to non-pressure-treated regions but the organization was still disturbed, in non-pressure-treated regions, elastin was present in patch deposits;in pressure-treated regions elastin formed fibers, smaller than in normal dermis. Tenascin was present in the whole dermis in non-pressure-treated regions, whereas in pressure-treated regions it was observed only under epidermis and around vessels, as in normal skin. alpha-Smooth muscle actin-expressing myofibroblasts were absent in normal skin, present in large amounts in non-pressure-treated regions, and almost absent in pressure-treated regions. The disturbed ultrastructural organization of dermal-epidermal junction observed in non-pressure-treated regions disappeared after pressure therapy;typical features of apoptosis in fibroblastic cells and morphological aspects of collagen degradation were observed in pressure-treated regions. Our results show that, in hypertrophic scars, pressure therapy restores in part the extracellular matrix organization observed in normal scar and induces the disappearance of alpha-smooth muscle actin-expressing myofibroblasts, probably by apoptosis. We suggest that the pressure acts by accelerating the r

关键词：瘢痕肥大性/病理学显微镜检查电子皮肤/病理学皮肤/超微结构应力物理伤口愈合青少年成年人女(雌)性人类男(雄)性中年人

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Ampullary organ morphology of freshwater salmontail catfish, Arius graeffei

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JOURNAL OF MORPHOLOGY 2000年第2期246卷 142-149页

作者： Whitehead, DL Tibbetts, IR Daddow, LYM Univ Queensland Ctr Marine Studies Brisbane Qld 4072 Australia Univ Queensland Dept Zool & Entomol Brisbane Qld Australia

Two types of ampullary organs are present in the skin of the freshwater salmontail catfish, Arius graeffei, each consisting of a short canal (0.2-0.5 mm) oriented perpendicular to the basement membrane and ending in an ampulla. Histochemical staining techniques (Alcian blue and Lillie's allochrome) indicate that the ampullary canals contain an acidic mucopolysaccharide gel, which is uniform in its staining properties along the canals. Type II ampullary organs consist of a canal, the wall of which is lined with cuboidal epithelial cells. The canal opens into an ampulla with 50-60 receptor cells. Electron microscopy reveals that the pear-shaped receptor cells bear microvilli on their luminal surface and lie adjacent to an unmyelinated neuron. Type III ampullary organs differ from Type II in that the canal wall consists of cells that;possess a protein-rich sac at the luminal apex and have a polymorphic nucleus. The canals of Type III ampullary organs open to an ampulla with 8-30 receptor cells similar in both staining properties and structure to those of the Type II organ. In both types of ampullary organs, supportive cells surround each receptor cell except at the apex of the receptor cell. J. Morphol. 246:142-149, 2000. (C) 2000 Wiley-Liss, Inc.

关键词：鲇鱼类/解剖学和组织学鲇鱼类/生理学淡水葡糖氨基聚糖类/分析皮肤/解剖学和组织学皮肤/化学皮肤/细胞学皮肤/超微结构动物

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