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MORPHINE-LIKE PEPTIDES IN MAMMALIAN BRAIN - ISOLATION, STRUCTURE ELUCIDATION, AND INTERACTIONS WITH OPIATE RECEPTOR

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1976年第7期73卷 2515-2519页

作者： SIMANTOV, R SNYDER, SH JOHNS HOPKINS UNIV SCH MED DEPT PHARMACOL & EXPTL THERAPEUTICS BALTIMORE MD 21205 USA JOHNS HOPKINS UNIV SCH MED DEPT PSYCHIAT & BEHAV SCI BALTIMORE MD 21205 USA

A substance that competes for opiate receptor binding was isolated from calf brain and identified as 2 pentapeptides, H-Tyr-Gly-Gly-Phe-Met-OH (methionine enkephalin) and H-Tyr-Gly-Gly-Phe-Leu-OH (leucine enkephalin), with about 4 times more leucine enkephalin than methionine enkephalin. Na and Mg effects on opiate receptor interactions show that both peptides are agonists, whereas leucine enkephalin may be a purer agonist than methionine enkephalin.

关键词：氨基酸序列氨基酸类/分析结合竞争性脑化学配体锰/药理学纳洛酮/代谢神经组织蛋白质类/分析神经组织蛋白质类/分离和提纯寡肽类/分析寡肽类/分离和提纯寡肽类/代谢受体药物/药物作用钠/药理学动物牛

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Innervation of nociceptors in the menisci of the knee joint: an immunohistochemical study

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ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY 2000年第3-4期120卷 201-204页

作者： Mine, T Kimura, M Sakka, A Kawai, S Yamaguchi Univ Sch Med Dept Orthoped Surg Yamaguchi Japan

Using histology, we studied the innervation of nociceptors in the medial and lateral menisci of the knee joint. Specimens examined were taken from 16 patients during arthroplasty. The patients were 6 men and 10 women, with ages ranging from 14 to 76 years (mean 56 years). Immunohistochemistry with the unlabeled antibody biotin-streptavidin method was employed to detect protein gene product 9.5 (PGP 9.5) or substance P (SP) in the specimen. The antibody for PGP 9.5 detected nerve tissues in the menisci, Most but not all of the nerve fibers were associated with blood vessels. Nerve fibers and sensory receptors were found mainly in the peripheral, vascular zone, representing the outer one-third of the meniscus, and the innervated area was wider in the anterior and posterior hems. Pacinian and Ruffini corpuscles as well as free nerve endings were identified in these areas. Larger fibers coursed circumferentially in the peripheral zone, with smaller branches of nerve fibers running radially into the meniscus. Nerve fibers positive for SP were also detected in the menisci, but were fewer in number. Their branches also were fewer, oriented radially and paralleling blood vessels. This study showed that some of the pain in cases of meniscal tear could originate in the meniscus itself, especially with peripheral tears that may be accompanied by bleeding.

关键词：关节成形术置换膝组织学技术免疫组织化学机械感受器/生理学半月板胫骨/损伤半月板胫骨/神经支配神经纤维/代谢神经组织蛋白质类/分析疼痛感受器/解剖学和组织学疼痛感受器/代谢疼痛感受器/生理学环层小体/生理学 P物质/分析硫酯水解酶类/分析泛素硫酯酶青少年成年人老年人女(雌)性人类男(雄)性中年人

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TRANSLATION INVITRO OF RAT-BRAIN MESSENGER-RNA CODING FOR TUBULIN AND ACTIN

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1975年第2期72卷 701-705页

作者： GOZES, I SCHMITT, H LITTAUER, UZ WEIZMANN INST SCI DEPT BIOCHEM REHOVOTH ISRAEL

A partially purified fraction of poly(a)-rich brain mRNA coding for tubulin and actin was obtained by stepwise elution from an oligo(deoxythymidylate)-cellulose column and was efficiently translated in a wheat-germ cell-free system. The newly synthesized tubulin and actin migrated along with the authentic proteins on sodium dodecyl sulfate polyacrylamide gels and on sodium dodecyl sulfate-urea polyacrylamide gels, where tubulin alpha- and beta-subunits are separated. The two proteins synthesized in vitro were found to be biologically active; they could be induced to polymerize and were both precipitated by vinblastine. In addition, specific binding of tubulin to an affinity column of colchicine-Sepharose and actin to myosin were demonstrated.

关键词：肌动蛋白类/生物合成脑/代谢脑/超微结构细胞分级分离无细胞系统化学沉淀色谱法亲和电泳聚丙烯酰氨凝胶动力学微管神经组织蛋白质类/分析神经组织蛋白质类/生物合成植物多核糖体/代谢蛋白质生物合成 RNA 信使/代谢长春碱动物大鼠

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IMMUNOFLUORESCENCE LOCALIZATION OF OLFACTORY MARKER PROTEIN

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BRAIN RESEARCH 1975年第1期96卷 176-180页

作者： HARTMAN, BK MARGOLIS, FL WASHINGTON UNIV SCH MEDDEPT PSYCHIATST LOUISMO 63110 ROCHE INST MOLEC BIOL NUTLEYNJ 07110

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7S NERVE GROWTH-FACTOR PROTEIN IN GOLDEN-HAMSTER

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JOURNAL OF NEUROCHEMISTRY 1976年第6期27卷 1315-1318页

作者： SHINE, HD PEREZPOLO, JR UNIV TEXAS DEPT ZOOL AUSTIN TX 78712 USA UNIV TEXAS MED BRANCH INST MARINE BIOMED GALVESTON TX 77550 USA

Levels of 7S nerve growth factor [NGF] in various tissues were measured using a new assay procedure. The assay consisted of labeling native 7S NGF by allowing an 125I-labeled subunit ([125I]-.alpha.) of 7S NGF to compete with the native .alpha. subunit. The presence of 7S NGF was noted in a wide variety of non-neuronal and neuronal tissues. Levels of 7S NGF in the CNS decreased as the distance from the metencephalon increased both rostrally and caudally.

关键词：结合竞争性脑化学仓鼠亚科电泳聚丙烯酰氨凝胶碘放射性同位素/诊断应用金仓鼠方法神经生长因子类/分析神经生长因子类/代谢神经组织蛋白质类/分析神经组织蛋白质类/代谢动物小鼠

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ISOLATION, PRIMARY STRUCTURE, AND SYNTHESIS OF ALPHA-ENDORPHIN AND GAMMA-ENDORPHIN, 2 PEPTIDES OF HYPOTHALAMIC-HYPOPHYSIAL ORIGIN WITH MORPHINOMIMETIC ACTIVITY

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1976年第11期73卷 3942-3946页

作者： LING, N BURGUS, R GUILLEMIN, R SALK INST LA JOLLA CA 92037 USA

The isolation and primary structure of 2 peptides with morphinomimetic activity, obtained from an extract of porcine hypothalamus-neurohypophysis, are described. The amino acid sequence of the 2 peptides, named .alpha.-endorphin and .gamma.-endorphin, was determined by mass spectrometry and dansyl-Edman methods to be H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-OH and H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-OH, respectively. These correspond to the amino acid sequences present between residues 61 and 76 and residues 61 and 77 of the various .beta.-lipotropins. A 3rd peptide also obtained in pure form in these studies was an unstable salt of .alpha.-endorphin.

关键词：氨基酸序列下丘脑/化学神经组织蛋白质类/分析神经组织蛋白质类/化学合成肽类/分析肽类/化学合成垂体后叶/化学猪动物

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A procedure for the bulk preparation of brain microsomes with low speed centrifugation.

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Preparative biochemistry 1976年第6期6卷 489-501页

作者： GuarnieriM FinneganA DeMarchenaO [a]Neurochemistry Laboratories Department of Neurology Johns Hopkins Medical School Baltimore MD. 21205

A method is described for the rapid isolation of microsomes from brains of various species. The method depends on the interaction of calcium with microsomes. The procedure is limited because calcium inhibits certain microsomal enzymes. Magnesium and manganese may be substituted for calcium. However, the substitutions decrease the reliability and efficiency of the procedure.

关键词：脑/超微结构脑化学钙阳离子二价阳离子一价细胞分级分离/方法离心法胆固醇/分析细胞色素还原酶/分析镁锰微粒体/分析微粒体/超微结构神经组织蛋白质类/分析 RNA/分析动物大鼠

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Abnormal dentatorubral-pallidoluysian atrophy (DRPLA) protein complex is pathologically ubiquitinated in DRPLA brains

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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1999年第1期260卷 133-138页

作者： Yazawa, I Nakase, H Kurisaki, H Toranomon Gen Hosp Dept Neurol Minato Ku Tokyo 1058470 Japan Okinaka Mem Inst Med Res Minato Ku Tokyo 1058470 Japan Natl Tokyo Hosp Dept Neurol Kiyose Tokyo 2048585 Japan

Dentatorubral-pallidoluysian atrophy (DRPLA) is caused by expansion of a glutamine repeat in DRPLA protein. DRPLA protein undergoes greater complex formation in DRPLA brain tissue, and expanded glutamine repeat enhances complex formation of DRPLA protein. Immunoblots with and without reduction show that the DRPLA protein complex is ubiquitinated only in DRPLA brain tissue. Moreover, immunoblots of regional DRPLA brain tissues reveal that pathological ubiquitination of DRPLA protein complex is found selectively in affected lesions. Double-labeling immunohistochemical studies with antibodies against DRPLA protein and ubiquitin demonstrate that the DRPLA protein is co-localized with ubiquitin in DRPLA neurons and show characteristic neuronal cytoplasmic inclusions with ubiquitinated DRPLA protein complex in the center. Our findings suggest that DRPLA protein undergoes abnormal complex formation with expanded glutamine repeat, and then the complex is pathologically ubiquitinated in DRPLA brain tissue. Pathological ubiquitination of abnormal DRPLA protein complex plays a role in DRPLA pathology. (C) 1999 Academic Press.

关键词：萎缩脑/病理学小脑核/病理学苍白球/病理学免疫组织化学神经组织蛋白质类/分析神经组织蛋白质类/化学神经组织蛋白质类/代谢蛋白质折叠组织分布泛素类/分析泛素类/生理学成年人老年人人类中年人

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Perinatal ontogeny of brain growth in the domestic pig

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Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) 2000年第1期223卷 102-8页

作者： W G Pond S L Boleman M L Fiorotto H Ho D A Knabe H J Mersmann J W Savell D R Su USDA/ARS Children's Nutrition Research Center Houston Texas 77030 USA. wgp3@cornell.edu

The perinatal development of the brain is highlighted by a growth spurt whose timing varies among species. The growth of the porcine cerebrum was investigated from the third trimester of gestation (70 days postconception) through the first 3.5 weeks of postnatal life (140 days postconception). The shape of the growth curves for cerebrum weight, total protein mass, total cell number (estimated by DNA content), and myelination (estimated by cholesterol accretion) were described. The growth velocity of cerebrum weight had two peaks, one at 90 days and the other at 130 days postconception, whereas that of total protein was greatest from 90 to 130 days postconception, and that of total DNA was greatest between 90 and 110 days and again at 130 days postconception. The growth velocity for total cholesterol continued to increase during the entire period, suggesting that myelination continued after the growth spurts for cells (protein and DNA). The growth velocity patterns observed in these contemporary pigs suggest that this species may be an appropriate model for human brain development, not only in the perinatal pattern of increase in mass of the cerebrum, as established previously, but also with regard to the patterns of cellular development and myelination.

关键词：衰老体重脑/胚胎学脑/生长和发育脑化学小脑/胚胎学小脑/生长和发育胆固醇/代谢 DNA/分析胚胎和胎儿发育胎儿孕龄髓鞘/生理学神经组织蛋白质类/分析器官测量女(雌)性人类妊娠

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