Investigates the potential relationship between the extracellular matrix protein Slit and Robo as receptor in the extracellular matrix. Binding to Robo-expressing cells in fly and vertebrate proteins; Slit's repul...
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Investigates the potential relationship between the extracellular matrix protein Slit and Robo as receptor in the extracellular matrix. Binding to Robo-expressing cells in fly and vertebrate proteins; Slit's repulsion of motor and olfactory bulb axons; Promotion of axonal branching.
作者：
SHASHOUA, VEMailman Research Center
McLean Hospital Department of Biological Chemistry Harvard Medical School Belmont Mass. 02178 U.S.A.
Immunochemical and double labeling experiments were used to demonstrate that at least 2 out of 3 brain cytoplasmic proteins, whose metabolism is markedly influenced by behavior, are products which are secreted into th...
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Immunochemical and double labeling experiments were used to demonstrate that at least 2 out of 3 brain cytoplasmic proteins, whose metabolism is markedly influenced by behavior, are products which are secreted into the extracellular fluid (ECF) of goldfish [Carassius auratus] brain. Even after 1 h of labeling, the extracts of goldfish brains with 0.32 M sucrose contained highly labeled proteins. Electrophoretic analyses of the proteins, on SDS [sodium dodecyl sulfate] polyacrylamide gels, indicated that an increased incorporation of [3H]valine occurs for trained animals as compared to untrained controls ([14C]valine) at specific protein bands migrating at 32,000 and 26,000 daltons. The proteins in the ECF gave identical precipitin bands to goldfish brain proteins whose metabolism was responsive to the acquisition of new patterns of behavior and to proteins in CSF. These results are consistent with the hypothesis that the cells which contain the proteins and whose location in the ependymal zone was determined by immunohistochemistry can secrete the products into ECF. The functional sites of the proteins are possibly away from the locus of their synthesis.
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