检索结果-内蒙古大学图书馆

Functional domains within the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels

JOURNAL OF PHYSIOLOGY-LONDON 1999年第3期520卷 631-644页

作者： Benos, DJ Stanton, BA Univ Alabama Birmingham Dept Physiol & Biophys Birmingham AL 35294 USA Dartmouth Med Sch Dept Physiol Hanover NH 03755 USA

Application of recombinant DNA technology and electrophysiology to the study of amiloride-sensitive Naf channels has resulted in an enormous increase in the understanding of the structure-function relationships of these channels. Moreover, this knowledge has permitted the elucidation of the physiological roles of these ion channels in cellular processes as diverse as transepithelial salt and water movement, taste perception, volume regulation, nociception, neuronal function, mechanosensation, and even defaecation. Although members of this ever-growing superfamily of ion channels (the Deg/ENaC superfamily) share little amino acid identity, they are all organized similarly, namely, two short N- and C-termini, two short membrane-spanning segments, and a very large extracellular loop domain. In this brief Topical Review, we discuss the structural features of each domain of this Deg/ENaC superfamily and, using ENaC as a model, show how each domain relates to overall channel function.

关键词：氨基酸序列/遗传学细胞质/代谢离子通道/遗传学离子通道/生理学分子序列数据神经组织蛋白质类/遗传学神经组织蛋白质类/生理学钠通道/遗传学钠通道/生理学动物

来源：评论

学校读者我要写书评

暂无评论

REGULATION OF TUBULIN EXPRESSION THROUGH CELL-CYCLE

引用

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1975年第JUN30期253卷 292-303页

作者： KLEVECZ, RR FORREST, GL CITY HOPE NATL MED CTR DEPT CELL BIOL DUARTE CA 91010 USA

来源：评论

学校读者我要写书评

暂无评论

Expression of a novel RNA-splicing factor, RA301/Tra2β, in vascular lesions and its role in smooth muscle cell proliferation

引用

AMERICAN JOURNAL OF PATHOLOGY 2001年第5期158卷 1685-1694页

作者： Tsukamoto, Y Matsuo, N Ozawa, K Hori, O Higashi, T Nishizaki, J Tohnai, N Nagata, I Kawano, K Yutani, C Hirota, S Kitamura, Y Stern, DM Ogawa, S Natl Cardiovasc Ctr Dept Pathol Suita Osaka 5658565 Japan Natl Cardiovasc Ctr Dept Neurosurg Suita Osaka 5658565 Japan Dainippon Pharmaceut Co Ltd R&D Operat Suita Osaka Japan Kanazawa Univ Sch Med Dept Neuroanat Kanazawa Ishikawa 920 Japan Osaka Rosai Hosp Dept Pathol Sakai Osaka Japan Osaka Univ Grad Sch Med Dept Pathol Suita Osaka Japan Columbia Univ Coll Phys & Surg Dept Physiol & Cellular Biophys New York NY 10032 USA

RA301/Tra2 beta, a sequence-specific RNA-binding protein, was first cloned as a stress molecule in re-oxygenated astrocytes. In human vascular tissues, we have found enhanced RA301/Tra2 beta expression in coronary artery with intimal thickening, and atherosclerotic aorta. Balloon injury to the rat carotid artery induced RA301/Tra2 beta transcripts followed by expression of the antigen, which was detected in medial and neointimal vascular smooth muscle cells (VSMCs). In cultured VSMCs, hypoxia/re-oxygenation caused induction of RA301/Tra2 beta and was accompanied by cell proliferation, both of which were blocked by the addition of either diphenyl iodonium, a NADPH oxidase inhibitor, PD98059, a mitogen-activated protein kinase kinase inhibitor, or antisense oligonucleotide for RA301/Tra2 beta, Consistent with a link between RA301/Tra2 beta and cell proliferation, platelet-derived growth factor also induced expression of RA301/Tra2 beta in cultured VSMCs. These data suggest a possible role for RA301/Tra2 beta in the regulation of VSMC proliferation, especially in the setting of hypoxia/reoxygenation-induced cell stress.

关键词：联苯化合物/药理学颈动脉/代谢颈动脉/病理学细胞分裂/药物作用细胞分裂/生理学 DNA 反义/药理学剂量效应关系药物类黄酮物质/药理学基因表达肌平滑血管/细胞学肌平滑血管/药物作用肌平滑血管/代谢神经组织蛋白质类/遗传学神经组织蛋白质类/生理学鎓类化合物/药理学氧/药理学 RNA剪接 RNA 信使/遗传学 RNA 信使/代谢 RNA结合蛋白质类/遗传学 RNA结合蛋白质类/生理学大鼠 Sprague-Dawley 超氧化物类/代谢青少年成年人老年人动物女(雌)性人类婴儿男(雄)性中年人大鼠

来源：评论

学校读者我要写书评

暂无评论

Regulation of neuregulin signaling by PSD-95 interacting with ErbB4 at CNS synapses

引用

NEURON 2000年第2期26卷 443-455页

作者： Huang, YZ Won, S Ali, DW Wang, Q Tanowitz, M Du, QS Pelkey, KA Yang, DJ Xiong, WC Salter, MW Mei, L Univ Alabama Birmingham Dept Neurobiol Pathol & Phys Med & Rehabil Birmingham AL 35294 USA Univ Virginia Dept Pharmacol Charlottesville VA 22908 USA Chinese Acad Sci Inst Neurosci Mol Neurobiol Lab Shanghai 200031 Peoples R China Shanghai Res Ctr Life Sci Shanghai 200031 Peoples R China Univ Toronto Hosp Sick Children Dept Physiol Program Brain & Behav Toronto ON M5G 1X8 Canada Univ Toronto Hosp Sick Children Dept Physiol Cell Biol Program Toronto ON M5G 1X8 Canada Georgetown Univ Vincent T Lombardi Canc Res Ctr Washington DC 20007 USA Univ Alabama Birmingham Dept Pathol Birmingham AL 35294 USA

Neuregulins (NRGs) and their receptors, the ErbB protein tyrosine kinases, are essential for neuronal development, but their functions in the adult CNS are unknown. We report that ErbB4 is enriched in the postsynaptic density (PSD) and associates with PSD-95. Heterologous expression of PSD-95 enhanced NRG activation of ErbB4 and MAP kinase. Conversely, inhibiting expression of PSD-95 in neurons attenuated NRG-mediated activation of MAP kinase. PSD-95 formed a ternary complex with two molecules of ErbB4, suggesting that PSD-95 facilitates ErbB4 dimerization. Finally, NRG suppressed induction of long-term potentiation in the hippocampal CA1 region without affecting basal synaptic transmission. Thus, NRG signaling may be synaptic and regulated by PSD-95. A role of NRG signaling in the adult CNS may be modulation of synaptic plasticity.

关键词：脑/细胞学脑/生理学细胞培养的电刺激海马/细胞学海马/代谢海马/生理学细胞内信号肽和蛋白质类长时程增强/药物作用膜蛋白质类神经组织蛋白质类/代谢神经组织蛋白质类/生理学神经调节蛋白类/药理学神经调节蛋白类/生理学神经元/代谢信号传导/生理学突触/生理学组织分布酵母菌动物大鼠

来源：评论

学校读者我要写书评

暂无评论

Bone morphogenetic proteins are required in vivo for the generation of sympathetic neurons

引用

NEURON 1999年第4期24卷 861-870页

作者： Schneider, C Wicht, H Enderich, J Wegner, M Rohrer, H Max Planck Inst Hirnforsch Neurochem Abt D-60528 Frankfurt Germany Univ Frankfurt Klinikum D-60590 Frankfurt Germany Univ Hamburg Klinikum Eppendorf Zentrum Mol Neurobiol D-20246 Hamburg Germany

Bone morphogenetic proteins (BMPs) induce autonomic neurogenesis in neural crest cultures and stimulate sympathetic neuron development when overexpressed in vivo. We demonstrate that inhibition of BMPs in the chick embryo by the BMP antagonist Noggin prevents sympathetic neuron generation. In Noggin-treated embryos, the noradrenergic marker genes tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), panneuronal neurofilament 160 (NF160) and SCG10 genes, and the transcriptional regulators Phox2b and Phox2a are not expressed in sympathetic ganglia. Whereas initial ganglion development is not affected, the expression of the basic helix-loop-helix transcription factor cash-1 is strongly reduced. These results demonstrate that BMPs are essential for sympathetic neuron development and establish Cash-1 and Phox2 genes as downstream effecters of BMPs in this lineage.

关键词：载体蛋白质类细胞分化/生理学细胞培养的 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/生理学神经节/细胞学神经节/生理学高迁移率族蛋白质类/遗传学同源盒结构域蛋白质类/遗传学同源盒结构域蛋白质类/生理学免疫组织化学原位杂交原位缺口末端标记神经组织蛋白质类/遗传学神经组织蛋白质类/生理学神经嵴/细胞学神经嵴/生理学神经元/生理学去甲肾上腺素/生理学蛋白质类/药理学交感神经系统/细胞学交感神经系统/生理学转录因子/遗传学转录因子/生理学转录遗传转化生长因子β 鸡胚

来源：评论

学校读者我要写书评

暂无评论

Neuropeptide FF and FMRFamide potentiate acid-evoked currents from sensory neurons and proton-gated DEG/ENaC channels

引用

NEURON 2000年第1期26卷 133-141页

作者： Askwith, CC Cheng, C Ikuma, M Benson, C Price, MP Welsh, MJ Univ Iowa Coll Med Howard Hughes Med Inst Iowa City IA 52242 USA Univ Iowa Coll Med Dept Internal Med Iowa City IA 52242 USA Univ Iowa Coll Med Dept Physiol & Biophys Iowa City IA 52242 USA

Acidosis is associated with inflammation and ischemia and activates cation channels in sensory neurons. Inflammation also induces expression of FMRFamide-like neuropeptides, which modulate pain. We found that neuropeptide FF (Phe-Leu-Phe-G In-Pro-Gln-Arg-Phe amide) and FMRFamide (Phe-Met-Arg-Phe amide) generated no current on their own but potentiated H+-gated currents from cultured sensory neurons and heterologously expressed ASIC and DRASIC channels. The neuropeptides slowed inactivation and induced sustained currents during acidification. The effects were specific;different channels showed distinct responses to the various peptides. These results suggest that acid-sensing ion channels may integrate multiple extracellular signals to modify sensory perception.

关键词：动作电位/药物作用动作电位/生理学细胞培养的 FMRF酰胺/药理学 FMRF酰胺/生理学神经节脊/药物作用神经节脊/生理学离子通道/药物作用离子通道/生理学神经组织蛋白质类/药物作用神经组织蛋白质类/生理学神经肽类/药理学神经肽类/生理学寡肽类/药理学寡肽类/生理学爪蟾属动物人类大鼠

来源：评论

学校读者我要写书评

暂无评论

Altered trafficking of membrane proteins in Purkinje cells of SCA1 transgenic mice

引用

AMERICAN JOURNAL OF PATHOLOGY 2001年第3期159卷 905-913页

作者： Skinner, PJ Vierra-Green, CA Clark, HB Zoghbi, HY Orr, HT Univ Minnesota Inst Human Genet Minneapolis MN 55455 USA Univ Minnesota Dept Lab Med & Pathol Minneapolis MN 55455 USA Univ Minnesota Dept Genet Cell Biol & Dev Minneapolis MN 55455 USA Univ Minnesota Dept Biochem Mol Biol & Biophys Minneapolis MN 55455 USA Baylor Coll Med Howard Hughes Med Inst Dept Mol & Human Genet Houston TX 77030 USA

Spinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disease caused by the expression of mutant ataxin-1 that contains an expanded polyglutamine tract. Overexpression of mutant ataxin-1 in Purkinje cells of transgenic mice results in a progressive ataxia and Purkinje cell pathology that are very similar to those seen in SCA1 patients. Two prominent aspects of pathology in the SCA1 mice are the presence of cytoplasmic vacuoles and dendritic atrophy. We found that the vacuoles;in Purkinje cells seem to originate as large invaginations of the outer cell membrane. The cytoplasmic vacuoles;contained proteins from the somatodendritic membrane, Including mGluR1, GluR Delta1/Delta2, GluR2/3, and protein kinase C (PKC) gamma. Further examination of PKC gamma revealed that its sequestration into cytoplasmic vacuoles was accompanied by concurrent loss of PKC gamma localization at the Purkinje cell dendritic membrane and decreased detection of PKC gamma by Western blot analysis. In addition, the vacuoles were immunoreactive for components of the ubiquitin/proteasome degradative pathway. These findings present a link between vacuole formation and loss of dendrites in Purkinje cells of SCA1 mice and Indicate that altered somatodendritic membrane trafficking and loss of proteins including PKC gamma, are a part of the neuronal dysfunction in SCA1 transgenic mice.

关键词：半胱氨酸内肽酶类/代谢细胞质/代谢细胞质/超微结构树突/代谢细胞内膜/代谢同工酶类/代谢膜蛋白质类/代谢小鼠转基因/遗传学多酶复合物/代谢神经组织蛋白质类/遗传学神经组织蛋白质类/代谢神经组织蛋白质类/生理学核蛋白质类/遗传学核蛋白质类/生理学蛋白酶体内肽酶复合物蛋白激酶C/代谢浦肯野细胞/代谢受体亲代谢性谷氨酸盐/代谢组织分布泛素类/代谢动物小鼠

来源：评论

学校读者我要写书评

暂无评论

Ionic interactions in the Drosophila serotonin transporter identify it as a serotonin channel

引用

NATURE NEUROSCIENCE 1999年第7期2卷 605-610页

作者： Petersen, CI DeFelice, LJ Vanderbilt Univ Med Ctr Dept Pharmacol Nashville TN 37232 USA Vanderbilt Univ Med Ctr Ctr Mol Neurosci Nashville TN 37232 USA

Serotonin transporters (SERTs) are targets for drugs such as Prozac that increase serotonin (5HT) levels by blocking 5HT reuptake. Although SERTs saturate in the micromolar range, synaptic 5HT may exceed 1 mM. To examine SERT's response to high 5HT concentrations, we expressed Drosophila SERT (dSERT) in Xenopus oocytes and found that transport continued to increase with concentration up to 0.3 mM 5HT. As 5HT is a monovalent cation, its entry through an ion channel in SERT might explain uptake at high concentrations. We therefore investigated dSERT using traditional ion channel methods, including mole-fraction experiments under voltage clamp. We propose that SERTs may function as 5HT-permeable channels, and that this mechanism may be important for clearance of the neurotransmitter at high concentrations.

关键词：载体蛋白质类/遗传学载体蛋白质类/生理学果蝇属/生理学果蝇蛋白质类锂/药理学膜糖蛋白类/遗传学膜糖蛋白类/生理学膜电位/药物作用膜电位/生理学膜转运蛋白质类神经组织蛋白质类/生理学卵母细胞/生理学帕罗西汀/药理学膜片钳术血清素/代谢血清素/药理学血清素/生理学血清素质膜转运蛋白质类钠/药理学爪蟾光滑动物女(雌)性大鼠

来源：评论

学校读者我要写书评

暂无评论

Aberrant development of motor axons and neuromuscular synapses in erbB2-deficient mice

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2000年第3期97卷 1299-1304页

作者： Lin, WC Sanchez, HB Deerinck, T Morris, JK Ellisman, M Lee, KF Salk Inst Biol Studies La Jolla CA 92037 USA Univ Calif San Diego Natl Ctr Microscopy & Imaging Res Dept Neurosci La Jolla CA 92093 USA

Receptor tyrosine kinase erbB2, which is activated by neuregulin, is expressed in Schwann and muscle cells in the developing neuromuscular junction (NMJ). in vitro studies have shown that neuregulin promotes the survival and migration of Schwann cells and stimulates acetylcholine receptor gene transcription in cultured muscle cells. These findings suggest an important role for erbB2 in the development of the NMJ. Here we examine erbB2-deficient mice to determine whether erbB2 is required for NMJ development in vivo. Our analysis shows that there are pre- and postsynaptic defects of developing NMJ in erbB2-deficient embryos. The presynaptic defects include defasciculation and degeneration of the motor nerves, and an absence of Schwann cells. The postsynaptic defect features an impairment of junctional folds at the neuromuscular synapse in the mutants. These results demonstrate that erbB2 is essential for in vivo development of the NMJ.

关键词：轴突/病理学细胞运动膈/胚胎学膈/病理学胚胎和胎儿发育基因表达调控发育期基因 erbB-2 原位杂交荧光肋间肌/胚胎学肋间肌/病理学小鼠基因敲除小鼠神经学突变型形态发生运动神经元/病理学肌蛋白质类/缺乏肌蛋白质类/遗传学肌蛋白质类/生理学神经变性神经组织蛋白质类/缺乏神经组织蛋白质类/遗传学神经组织蛋白质类/生理学神经调节蛋白类/生理学神经肌肉接头/畸形神经肌肉接头/胚胎学膈神经/胚胎学膈神经/病理学受体 erbB-2/缺乏受体 erbB-2/生理学受体胆碱能/生物合成受体胆碱能/遗传学许旺细胞/代谢许旺细胞/病理学动物小鼠

来源：评论

学校读者我要写书评

暂无评论

Trophic and tropic factors in the development of the central nervous system

引用

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY 1998年第3期106卷 161-202页

作者： Nagtegaal, ID Lakke, EAJF Marani, E Leiden Univ Dept Physiol LUMC Neuroregulat Grp NL-2300 RC Leiden Netherlands Leiden Univ LUMC Dept Pathol NL-2300 RC Leiden Netherlands

Click to increase image sizeClick to decrease image size

关键词：脑源性神经营养因子/生理学细胞黏附分子神经元/生理学中枢神经系统/胚胎学中枢神经系统/生长和发育中枢神经系统/生理学鸡/遗传学睫状神经营养因子细胞因子类/生理学果蝇属/遗传学表皮生长因子/生理学进化分子成纤维细胞生长因子/生理学生长物质/生理学小鼠基因敲除模型生物学神经生长因子类/生理学神经组织蛋白质类/生理学血小板源性生长因子/生理学信号素类生长调节素类/生理学转化生长因子α/生理学转化生长因子β/生理学肿瘤抑制蛋白质类动物人类小鼠

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：