检索结果-内蒙古大学图书馆

Effects of acute and subchronic administration of typical and atypical antipsychotic drugs on the neurotensin system of the rat brain

引用

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 2000年第1期295卷 67-73页

作者： Kinkead, B Shahid, S Owens, MJ Nemeroff, CB Emory Univ Sch Med Dept Psychiat & Behav Sci Lab Neuropsychopharmacol Atlanta GA 30322 USA

The acute and subchronic effects of a variety of doses of a prototype typical (haloperidol) or one of several atypical antipsychotic drugs (clozapine, olanzapine, risperidone, quetiapine, or sertindole) on regional brain neurotensin (NT) tissue concentrations, and NT receptor binding were examined. Acute administration of haloperidol, clozapine, olanzapine, and risperidone dose-dependently increased NT tissue concentrations in the nucleus accumbens. Haloperidol, olanzapine, risperidone, and sertindole also increased NT tissue concentrations in the caudate nucleus. NT tissue concentrations in the nucleus accumbens and caudate remained elevated after 14-day administration of haloperidol, olanzapine, sertindole, and risperidone. In contrast, at the doses studied, quetiapine decreased NT tissue concentrations in the nucleus accumbens;clozapine had no effect. Haloperidol significantly increased NT receptor binding in the substantia nigra after 14-day administration. All of the atypical antipsychotic drugs decreased NT receptor binding in the nucleus accumbens and in the substantia nigra. Although these studies do not conclusively support the hypothesis that increased NT neurotransmission is involved in the clinically relevant effects of all antipsychotic drugs, the extant evidence clearly suggests that further study is warranted. Inconsistencies in the data suggest that differential effects of antipsychotic drug administration on subpopulations of NT neurons must be scrutinized further.

关键词：抗精神病药/药理学脑/药物作用脑/代谢剂量效应关系药物神经降压肽/分析大鼠 Sprague-Dawley 受体神经降压肽/药物作用受体神经降压肽/代谢动物男(雄)性大鼠

来源：评论

学校读者我要写书评

暂无评论

Agonist induced conformation alteration of neurotensin receptor and the mechanism behind NA⁺ inhibition of ¹²⁵I-NT binding

引用

JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH 1999年第6期19卷 995-1021页

作者： Mitra, SP Carraway, RE Blute, R Luber-Narod, J Univ Massachusetts Med Ctr Div Urol Worcester MA 01655 USA Univ Massachusetts Med Ctr Dept Physiol Worcester MA 01655 USA

In the absence of Na+, I-125-Neurotensin (I-125-NT) binding to the Neurotensin receptor (NTR) produces a stable noncovalent I-125-NT-NTR complex whose dissociation rate is extremely low even after the addition of 1 mu M NT, 100 mu M SR48692 (antagonist), 100 mu M GPPNHP or 100mM NaCl. Lowering the medium pH to 4.5 enhances the process (similar to 70% in 10 minutes). Labeling by photoactivatable I-125-Tyr(3)-Azo(4)-NT identifies a similar to 50 KD Mr band along with several ether minor components. Interestingly, the labeling intensity is drastically reduced when binding is performed in the presence of Na or GPPNHP. However, a minor reduction is noticed when Na+ or GPPNHP is added to the medium after binding. The binding kinetics indicates that Na+ lowers the rate of I-125-NT association by acting as a noncompetitive inhibitor. On the contrary, Na+ favors the interaction of antagonist, SR48692 by lowering the value of K-i. GTP gamma(35)S binding to membranes in the presence of 30mM NaCl suggests that Na+ inhibition of I-125-NT binding is due to the uncoupling of NTR associated G protein(s). In order to explain the entire phenomenon, a two-step, binding model has been proposed. In Step-1, interaction between NT and NTR produces a transient complex, which attains a stable state in the absence of NaCl via step-2, thereby altering the native NTR conformation. The presence of Na+ prevents step-2 by dissociating the transition complex.

关键词：结合部位细胞膜/代谢鸡肝/代谢神经降压肽/化学神经降压肽/代谢蛋白质结合蛋白质构象受体神经降压肽/化学受体神经降压肽/代谢钠/代谢动物

来源：评论

学校读者我要写书评

暂无评论

Protein 7B2 is essential for the targeting and activation of PC2 into the regulated secretory pathway of rMTC 6-23 cells

引用

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1999年第2期257卷 473-479页

作者： Barbero, P Kitabgi, P Univ Nice Sophia Antipolis CNRS Inst Pharmacol Mol & Cellulaire F-06560 Valbonne France

Among the prohormone convertases, PC2 is unique in that it specifically binds to the neuroendocrine-specific protein 7B2 in the endoplasmic reticulum (ER) and is activated late in the regulated secretory pathway of neuroendocrine cells. Several roles, sometimes contradictory, have been suggested for 7B2 with regard to PC2 cellular fate. Thus, 7B2 was proposed to act as a PC2 chaperone in the ER, or to facilitate 7B2 transport from the ER to the trans-Golgi network and to be necessary for proPC2 activation, or to inhibit PC2 enzymatic activity until the latter reaches the secretory granules. To gain insight into the function of 7B2, we sought to block its expression in PC2-expressing endocrine cells using antisense strategies. We have previously shown that the endocrine rMTC 6-23 cell line expresses PC2 and that the enzyme is responsible for the processing of pro-neurotensin/neuromedin N (proNT/NN). Here, we show that rMTC 6-23 cells express 7B2 and that the protein was coordinately induced with PC2 and proNT/NN by dexamethasone. Stable transfection of rMTC 6-23 cells with 7B2 antisense cDNA led to a marked reduction (>90%) in 7B2 levels. ProPC2 was expressed to normal levels and cleaved to yield a PC2 form that was constitutively released, was not stored within secretory granules and was unable to process proNT/NN. We conclude that 7B2 is essential for the sorting and activation of PC2 into the regulated secretory pathway of endocrine cells. (C) 1999 Academic Press.

关键词：印迹法蛋白质细胞系细胞质颗粒/代谢细胞质颗粒/分泌 DNA 反义/遗传学地塞米松/药理学减量调节内分泌腺/细胞学内分泌腺/分泌内质网/酶学内质网/代谢内质网/分泌酶激活酶诱导/药物作用免疫组织化学分子量神经组织蛋白质类/遗传学神经组织蛋白质类/代谢神经内分泌蛋白质7B2 神经降压肽/代谢垂体激素类/遗传学垂体激素类/代谢前蛋白转化酶2 蛋白质前体/代谢枯草杆菌蛋白酶类/代谢枯草杆菌蛋白酶类/分泌转染动物大鼠

来源：评论

学校读者我要写书评

暂无评论

Preparation and receptor binding affinities of cyclic C-terminal neurotensin (8-13) and (9-13) analogues

引用

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 1999年第17期9卷 2579-2582页

作者： Lundquist, JT Dix, TA Med Univ S Carolina Dept Pharmaceut Sci Charleston SC 29425 USA

Cyclic analogues of neurotensin (NT) C-terminal fragments NT(8-13) and NT(9-13) were produced via intramolecular nucleophilic substitution of the Tyr(11) phenoxide anion on a 6-bromohexanoyl side chain substituted at ... 详细信息

关键词：氨基酸序列神经降压肽/类似物和衍生物神经降压肽/化学合成神经降压肽/代谢肽碎片/化学合成肽碎片/代谢蛋白质结合受体神经降压肽/代谢人类

来源：评论

学校读者我要写书评

暂无评论

Synthesis of neurotensin(9-13) analogues exhibiting enhanced human neurotensin receptor binding affinities

引用

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 2000年第5期10卷 453-455页

作者： Lundquist, JT Büllesbach, EE Dix, TA Med Univ S Carolina Dept Pharmaceut Sci Charleston SC 29425 USA Med Univ S Carolina Dept Biochem & Mol Biol Charleston SC 29425 USA

Recent evidence is consistent with neurotensin (NT)(8-13) adopting a Type I beta-turn conformation while binding the NT receptor, which would place the cationic side-chains of Arg(8) and Arg(9) in close proximity. This was the basis for the design, synthesis and analysis of truncated NT(9-13) analogues 1-5 with dicationic position 9 side-chains to emulate the functions of the 8 and 9 side-chains of NT(8-13). (C) 2000 Published by Elsevier Science Ltd. All rights reserved.

关键词： CHO细胞色谱法高压液相仓鼠亚科分子量神经降压肽/类似物和衍生物神经降压肽/化学合成神经降压肽/化学神经降压肽/药理学肽碎片/化学合成肽碎片/化学肽碎片/药理学受体神经降压肽/代谢构效关系动物人类

来源：评论

学校读者我要写书评

暂无评论

EFFECTS OF CHRONIC ETHANOL ADMINISTRATION ON NEUROTENSINERGIC PROCESSES - CORRELATIONS WITH TOLERANCE IN LS AND SS MICE

引用

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1992年第1期654卷 441-443页

作者： ERWIN, VG CAMPBELL, AD RADCLIFFE, R Alcohol Research Center School of Pharmacy University of Colorado Boulder Colorado 80309-0297

来源：评论

学校读者我要写书评

暂无评论

肠管益生菌及微量喂养对早产儿血清表皮生长因子及神经降压素的影响

引用

陕西医学杂志 2011年第9期40卷 1143-1146页

作者：刘世平薛继红成钧延安大学附属医院儿科二病区延安716000

目的:探讨早期口服肠管益生菌及微量喂养对早产儿血清表皮生长因子(EGF)及神经降压素(NT)的影响。方法:将128例早产儿病例随机分为对照组36例、微量喂养组44例及混合喂养组(口服肠道益生菌及微量喂养组)48例。采用EL ISA法对3组早产儿生... 详细信息

目的:探讨早期口服肠管益生菌及微量喂养对早产儿血清表皮生长因子(EGF)及神经降压素(NT)的影响。方法:将128例早产儿病例随机分为对照组36例、微量喂养组44例及混合喂养组(口服肠道益生菌及微量喂养组)48例。采用EL ISA法对3组早产儿生后24h、72h及生后7d晨9时喂奶之前采血取血清,测定GEF及NT含量,并进行组间比较。结果:微量喂养组和混合喂养组GEF在生后24h、72h及生后7d呈持续上升趋势,混合喂养组生后7d GEF水平明显高于生后24h(P<0.01);微量喂养组生后24h与72h比较有极显著性差异(P<0.01),72h与生后7d比较有显著性差异(P<0.05);对照组GEF在生后3d最低,7d时增高,组间24h、72h及生后7d比较无显著性差异(P>0.05);3组在生后24h、72h及生后7d血清GEF含量不同(P<0.05)。3组NT在生后24h、72h及生后7d呈持续上升趋势,生后7d NT水平明显高于生后24h(P<0.01);微量喂养组和混合喂养组NT水平在生后24h、72h及生后7d比较无显著性差异(P>0.05),两组与对照组比较均有显著性差异(P<0.05)。结论:早期微量喂养可使早产儿血清内EGF及NT明显增高,减少喂养不耐受的发生,而口服益生菌虽不能使NT大量分泌,但可以帮助胃肠管尽快建立正常菌群,适应胃肠内营养,两者结合可以使NEC的发生率明显降低。

关键词：婴儿,早产生长物质/代谢神经降压肽/代谢 @肠管益生菌及微量喂养

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：