Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal *** had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly s...
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Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal *** had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub *** biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.
The insecticidal protein from Pseudomonas pseudoaligenes was and exotoxin which had toxicity on *** order to elucidate its molecular properties an amino acid sequence,the insecticidal protein was purified from the cul...
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The insecticidal protein from Pseudomonas pseudoaligenes was and exotoxin which had toxicity on *** order to elucidate its molecular properties an amino acid sequence,the insecticidal protein was purified from the culture supernatant by ultrafiltration,ion\|exchange chromatography and gel filtration,and showed a single band on SDS\|*** of the purified insecticidal protein dentified N\|terminal sequence of ten amino acid *** polyclonal antibody was also obtained by immunizing rabbit with the insecticidal protein recovered form SDS\|PAGE *** antibody titer determined by ELISA method was 1∶12800,indicating that it had high *** blot analysis revealed that the antibody was spectific to 26kD insecticidal protein,and did not cross\|react with other proteins produced by the bacterium,suggesting that a specific antibody with high titer was obtained and could be used for further investigations of the gene cloning and expression of insecticidal protein.
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