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INVITRO ELIMINATION OF OOCYTE MELANOSOMES FROM RETINAL RUDIMENT OF FROG, RANA-PIPIENS

EXPERIMENTAL EYE RESEARCH 1976年第2期22卷 129-140页

作者： HOLLYFIELD, JG COLUMBIA UNIV COLL PHYS & SURGDEPT OPHTHALMOLNEW YORKNY 10032

The behaviour of oocyte melanosomes in cultured retinal rudiments of Rana pipiens embryos was studied with light and electron microscopy. Following isolation from stage 17 embryos, oocyte melanosomes are mobilized to the apical surface of the developing retinal cells and expelled during a period of nine days in culture. Electron microscopic observations of melanosomes in stages of elimination indicate that the perimelanosomal membrane interacts with the plasma membrane prior to exteriorization of the melanosome.

关键词：细胞膜/生理学黑素细胞/生理学黑素细胞/超微结构卵母细胞/生理学卵母细胞/超微结构类器官/生理学类器官/超微结构卵子/生理学豹纹蛙视网膜/细胞学视网膜/胚胎学视网膜/超微结构动物女(雌)性

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ELECTRON-MICROSCOPY OF AGING CELLS OF PSEUDOMONAS-RHODOS - FINE-STRUCTURE OF NATIVE AND ISOLATED TUBULAR MEMBRANES

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ARCHIVES OF MICROBIOLOGY 1975年第3期104卷 215-223页

作者： ACKER, G SCHMITT, R MAYER, F UNIV ERLANGEN INST KLIN MIKROBIOL & INFEKTIONSHYGWASSERTURM STR 3D-8520 ERLANGENFED REP GER

During a 10 day-incubation on agar surfaces at 30 degrees C, cells of the gram-negative soil bacterium Pseudomonas rhodos pass through three phases distinguishable by physiological and morphological criteria. When viewed by electron microscopy, typically "rolled" mesosomes could frequently be observed in young cells. In aged cells instead, loosely rolled or stretched-out, flattened tubules could be discerned, presumed to be degenerate mesosomes. Tubular flattened structures have been isolated from these cells by lysozyme treatment or sonication and were concentrated by differential centrifugation. Electron micrographs of these preparations showed long, straight tubules which sometimes appeared sealed at one end. Their width was 34 +/- 5 nm. They contained a lining of material, which could be digested by trypsin leaving behind an electron-transparent matrix. In rare cases, isolated tubules showed a periodic fine structure composed of ellipsoidal subunits. Optical diffraction analysis yielded a lattice consisting of subunits arranged in helices of pitch-angle 27 degrees; the unit cell dimensions were shown to be 112 X 56 A. Owing to their sensitivity to trypsin, components of the regular lattice are supposed to consist of protein. It is postulated that these protein components are layered onto a tubular membrane. These tubules are clearly distinguishable by their shape and fine structure from the periodic structure of a P. rhodos cell wall layer, which exhibits a tetragonal pattern, and also from polyheads and polysheaths of defective bacteriophages. Their possible origin from intact mesosomes in discussed.

关键词：衰老细胞分级分离细胞壁/超微结构显微镜检查电子类器官/超微结构假单胞菌属/超微结构

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FINE-STRUCTURE AND X-RAY MICROANALYSIS OF MELANOSOMES IN PIGMENTED NEVI AND MELANOMAS

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ARCHIV FUR DERMATOLOGISCHE FORSCHUNG 1975年第4期252卷 297-304页

作者： SZEKERES, L MED UNIV SZEGED DEPT DERMATOLPOB 480H-6701 SZEGEDHUNGARY

Further finestructural characteristics of melanosomes in pigmented nevi and melanomas are described. The differences in the calcium, iron and sulphur content of melanosomes derived from melanocytes, nevocytes and mali... 详细信息

关键词：钙/分析电子探针微区分析铁/分析黑素类/生物合成黑素细胞/分析黑素细胞/超微结构黑色素瘤/分析黑色素瘤/诊断黑色素瘤/病理学痣色素/分析痣色素/诊断痣色素/病理学类器官/分析类器官/超微结构皮肤肿瘤/诊断皮肤肿瘤/病理学硫/分析人类

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ULTRASTRUCTURAL OBSERVATIONS ON A FOLLICULAR CARCINOMA OF THYROID-GLAND

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AMERICAN JOURNAL OF CLINICAL PATHOLOGY 1976年第3期65卷 328-336页

作者： KAY, S TERZ, JJ MED COLL VIRGINIA DEPT SURGRICHMONDVA 23298 MED COLL VIRGINIA DIV SURG PATHOLRICHMONDVA 23298

Electron microscopic studies were performed on a follicular carcinoma removed from a 31-year-old woman. The tumor was poorly differentiated and inoperable but distinctly follicular in pattern. Ultrastructurally, the neoplasm had many of the features of papillary carcinoma, follicular adenoma, and anaplastic carcinoma. Due to the presence of secretory granules it even somewhat resembled medullary carcinoma, but the granules were distinctly larger, and the production of amyloid was definitely excluded.

关键词：腺癌/病理学纤毛/超微结构溶酶体/超微结构显微镜检查电子类器官/超微结构甲状腺肿瘤/病理学成年人女(雌)性人类

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LIGHT AND DARK ADAPTATIONAL CHANGES IN ACCESSORY EYE OF SHRIMP, PALAEMONETES

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TISSUE & CELL 1976年第4期8卷 583-590页

作者： ITAYA, SK UNIV TENNESSEE CTR HLTH SCI MEMPHIS TN 38103 USA

The fine structure of the accessory eye of the shrimp Palaemonetes was studied after light and dark adaptation. Adjacent to the principal compound eye, the accessory eye is a small compound eye composed of .apprx. 20 ommatidia which are smaller than the ommatidia of the principal compound eye. During dark adaptation rhabdomal microvilli increase in length, but little pigment migration occurs. The implications of these changes in relation to the function of the accessory eye are discussed.

关键词：角膜/超微结构暗适应十足目(甲壳纲)/生理学十足目(甲壳纲)/超微结构内质网/超微结构眼/超微结构光眼生理学类器官/超微结构光感受器/超微结构色素类生物学动物

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VEILS, MOUNDS, AND VESICLE AGGREGATES IN NEURONS ELONGATING INVITRO

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EXPERIMENTAL CELL RESEARCH 1979年第1期119卷 163-174页

作者： NUTTALL, RP WESSELLS, NK STANFORD UNIV DEPT BIOL SCI STANFORD CA 94305 USA

Veils are thin membranous expanses spread between growth cone microspikes of living and fixed cells. Some veils lack vesicular contents, while others contain membranous sacs, tubules and vesicles. Mounds are bulges from the cell surface that are filled with vesicles: they are present on somas, along axonal surfaces and on growth cones of fixed cells. Transmission electron microscopy (TEM) of whole, unsectioned cultured chick embryo cells shows that in many cases the vesicles seen in thin sections are sacs or tubular structures, twisting in complex ways within the interior of a mound. Veils and mounds have certain distinctive characteristics when compared with adjacent neuronal regions. Cortical microfilamentous material is lacking beneath the plasma membrane of mounds and veils and between the vesicular or tubular contents of these structures. Cationic ferritin does not bind to the outer surface of mounds and veils. Experiments done to determine if fixation has effects on mound formation suggest that the final morphology of mounds may be induced by glutaraldehyde fixation.

关键词：细胞膜/超微结构细胞运动细胞培养的固定剂/药理学戊二醛/药理学神经元/药物作用神经元/生理学神经元/超微结构类器官/超微结构

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FINE-STRUCTURE OF MUSCLE-SPINDLES IN MASSETER MUSCLE OF RAT

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ARCHIVES OF ORAL BIOLOGY 1975年第7期20卷 451-+页

作者： HINRICHSEN, CFL STEVENS, GE UNIV TASMANIA DEPT ANAT HOBART TASMANIA AUSTRALIA

Because of the unique central organization of primary sensory neurons subserving jaw muscle spindles, a study of the fine structure of the spindles was undertaken to compare them with known features of spindles of somatic origin. Particular interest was directed toward fibre ultrastructure because of possible functional implications and toward sensory endings as neurons are known to outnumber the spindles they *** fibres classified as nuclear bag and nuclear chain in light micrographs did not show significant ultrastructural differences with respect to sarcomere length, size and distribution of mitochondria and presence of glycogen. Nuclear chain fibres possessed a higher concentration of dilated cisternae of sarcoplasmic reticulum. There was no evidence of cross innervation or indication of more than two morphologically distinct sensory endings.

关键词：糖原/分析咀嚼肌/神经支配咀嚼肌/超微结构线粒体肌/超微结构肌梭/超微结构肌原纤维/超微结构神经末梢/超微结构神经元/超微结构类器官/超微结构大鼠/解剖学和组织学肌浆网/超微结构动物女(雌)性

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VASCULAR STRUCTURES IN BRAIN TUMORS

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HUMAN PATHOLOGY 1975年第5期6卷 611-621页

作者： HIRANO, A MATSUI, T ALBERT EINSTEIN COLL MED 1300 MORRIS PK AVEBRONXNY 10461 MONTEFIORE HOSP & MED CTR DEPT PATHOLDIV NEUROPATHOLBRONXNY

The blood vessels within brain tumors show alterations from the normal anatomy. Some of these seem to be related to an increased capacity to transfer materials between the lumen and the parenchyma and are probably intimately connected with the edema associated with the tumor. These alterations include fenestration, widened intercellular junctions, increase in pinocytotic vesicles, and infolding of the luminal surface. Other alterations are observed but their function is not as clear. The latter include an increase in the number of tubular bodies, the appearance of tubular structures within vacuoles, tubular arrays within the nuclear envelope and rough endoplasmic reticulum, and endothelial proliferation, among others.

关键词：基膜/超微结构脑肿瘤/血液供给毛细血管/超微结构颅咽管瘤/血液供给无性细胞瘤/血液供给内皮/超微结构神经胶质瘤/血液供给细胞连接/超微结构髓母细胞瘤/血液供给微体/超微结构微管/超微结构类器官/超微结构胞饮作用空泡/超微结构人类

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LYMPHATIC TRANSPORT AND PHAGOCYTOSIS OF MELANOSOMES IN BLUE NEVUS

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ARCHIV FUR DERMATOLOGISCHE FORSCHUNG 1975年第4期252卷 239-244页

作者： SATO, S NISHIJIMA, A HIRAGA, K TOKYO WOMENS MED COLL DEPT DERMATOL TOKYO 162 JAPAN

The present paper desoribes ultrastructural aspect of lymphatic transport of dermal melanosomes in blue nevus. At least two processes may be responsible for the transport across the lymphatic endothelial wallin vivo; one is the passage of melanophages through the intercellular junctions and the another of free melanosomes. Phagocytosis of melanosomes by lymphatic endothelial cells is discussed.

关键词：生物转运毛细血管/超微结构毛细血管通透性上皮细胞上皮/超微结构细胞间接合部/超微结构淋巴系统/代谢淋巴系统/超微结构黑素细胞/超微结构痣色素/病理学类器官/超微结构吞噬细胞/超微结构吞噬作用皮肤肿瘤/病理学人类

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UNUSUAL CELL STRUCTURES IN TUMOR-LIKE FORMATIONS OF GRACILARIA (RHODOPHYTA)

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ARCHIVES OF MICROBIOLOGY 1976年第2期108卷 167-174页

作者： TRIPODI, G BETH, K UNIV NAPOLI FAC AGRARIA IST BOT I-80055 PORTICI ITALY STAZ ZOOL ANTON DOHRN I-80122 Naples ITALY

This paper deals with EM observations on cultivated plants of the marine red alga G. verrucosa which developed simple galls;also sea collected material, without galls, was studied. The galls showed unusual but characteristic cell structures, caterpillar-like bodies, containing rows of fusiform bodies. These were found mostly in the cytoplasm near the plastids, in one case connected with the endoplasmic reticulum, occasionally even inside the nucleus, and are probably described here for the first time. It does not seem probable that the caterpillar-like bodies represent mitochondria or bacteria, but the hypothesis that fusiform bodies are related to virus-like structures is discussed. The normal tissues as well as the gall tissue of the laboratory plants contained, besides plastids typical for the red algae, another type of plastids characterized by tubular thylakoids.

关键词：藻类红/超微结构细胞质颗粒/超微结构内质网/超微结构显微镜检查电子类器官/超微结构植物病毒

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