Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal *** had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly s...
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Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal *** had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub *** biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.
氨基酰化酶(Aminoacylase)是一种高分子量,双亚基的蛋白质酶,广泛分布于各种生物的各种组织中,是生物体内N端乙酰化氨基酸代谢过程中的一种重要水解酶,在中性pH值附近,氨基酰化酶在体外专一性催化N-乙酰-L氨基酸水解,当pH值升高时,氨基酰化酶也能催化多肽水解。氨基酰化酶Ⅰ最早是从动物的肝脏和肾脏中发现的,后来从曲霉菌发酵液中提取到了高效氨基酰化酶,从此开始了工业化生产。从植物中分离纯化此酶的报道仅见Lugay et al.从Palo Verde种子及施华芳等人从水稻黄化苗,绿化苗和种子提取两例,其它一些只是关于高等植物中氨基酰化酶分布的报道,从海藻中分离纯化此酶的研究尚未见报道。
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