Impression Cytology (IC) is a noninvasive and easily repeated technique for investigating many pathologies of the conjunctiva such as dry eye, drug toxicities, and melanoma. In addition to skin tests and determination...
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Impression Cytology (IC) is a noninvasive and easily repeated technique for investigating many pathologies of the conjunctiva such as dry eye, drug toxicities, and melanoma. In addition to skin tests and determination of serum IgE levels, the presence of eosinophilia in the nasal mucosa and the conjunctiva is an important diagnostic factor in allergic rhinoconjunctivitis. Eosinophilia can classically be shown by the nasal smear technique as well as IC. The nasal smear is a difficult test to carry out and does not always give adequate results. With this in mind, we collected cells from the nasal mucosa and superior palpebral conjunctiva from 27 patients with allergic rhinoconjunctivitis (17 female, IO male) using 5 X 5 mm, 0.22 mu m pore-sized cellulose acetate paper. The specimens were fixed in 95% alcohol and were then stained with hematoxylin eosin and periodic acid-Schiff stain for examination by light microscopy. Taking the free and intraepithelial eosinophilia into consideration, the specimens were grouped Of all patients, 92.6% and 85.2% had nasal and conjunctival eosinophilia, whereas 44.4% and 25.9% had nasal and conjunctival mononuclear cells, respectively. Our study has shown that IC is a very reliable and practical technique that offers a valuable alternative test for investigating both the intraepithelial and free eosinophilia in allergic rhinoconjunctivitis and other pathologies that cause cytologic changes.
To make an exfoliative cytological diagnosis, the specimens must contain enough cells for examination. To achieve this, a cell aspirator was designed consisting of a disposable syringe connected to a vacuum system and...
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To make an exfoliative cytological diagnosis, the specimens must contain enough cells for examination. To achieve this, a cell aspirator was designed consisting of a disposable syringe connected to a vacuum system and equipped with a cannula. When taking the specimen, physiological saline is applied drop by drop and the fluid is aspirated into the syringe together with the material from the lesion. This suspension is fixed and centrifuged, and the material in the pellet is transferred to a glass slide for staining and diagnosis. This cell aspirator technique produced specimens containing a large number of cells, which is a necessary condition for a reliable cytological diagnosis. An account is given of the results of a comparative histopathologic study of areas of the human oral mucosa in which cytological specimens were collected with a wooden spatula and with a cell aspirator. To judge from the histopathologic picture, the wooden spatula collected superficial epithelial cells with compression of the epithelium in the area of collection and leveling out of the cells in the direction of movement of the spatula. The cell aspirator collected cells from most layers of the epithelium and in a sharply circumscribed portion of the mucosal change. No subepithelial damage was observed with the 2 methods of collection.
The Millipore filter imprint technique was applied to oral cytology using modification of the Papanicolaou and Shorr stains. This technique has the major advantage of demonstrating on each imprint the topographic rela...
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The Millipore filter imprint technique was applied to oral cytology using modification of the Papanicolaou and Shorr stains. This technique has the major advantage of demonstrating on each imprint the topographic relationship between cellular zones and also allows for repeated sampling of superficial changes in the same area without tissue. This method provides a wide variety of cytological information about gingival anatomy and demonstrates a number of regions of differing cellular composition.
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