检索结果-内蒙古大学图书馆

Crystallization and preliminary crystallographic analysis of the archaeal intron-encoded endonuclease I-DmoI

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 1998年第6-2期54卷 1435-1436页

作者： Dalgaard, JZ Silva, GH Belfort, M Van Roey, P New York State Dept Hlth Wadsworth Ctr Albany NY 12201 USA SUNY Albany Sch Publ Hlth Dept Biomed Sci Albany NY 12201 USA

Two forms of the archaeal intron-encoded site-specific endonuclease I-DmoI, namely I-DmoIc and I-DmoIl, have been purified and crystallized. Crystals of I-DmoIc are rod-shaped and diffract to 3.0 Angstrom resolution, but further analysis was hampered by twinning. Crystals of I-DmoIl, which is a six-amino-acid C-terminal truncation of I-DmoIc, are plate shaped and belong to space group C2 with cell parameters a = 93.72, b = 37.03, c = 55.56 Angstrom, beta = 113.4 degrees, with one molecule per asymmetric unit (V-m = 2.01 Angstrom(3) Da(-1)). The crystals diffract to at least 2.3 Angstrom resolution. A complete native data set has been measured and structure determination is on-going.

关键词：细菌蛋白质类/化学细菌蛋白质类/分离和提纯结晶结晶学 X线脱氧核糖核酸酶类位点特异性Ⅰ型/化学脱氧核糖核酸酶类位点特异性Ⅰ型/分离和提纯脱硫球菌科/酶学大肠杆菌蛋白质构象重组融合蛋白质类/化学重组融合蛋白质类/分离和提纯

来源：评论

学校读者我要写书评

暂无评论

Crystallization and preliminary X-ray diffraction studies of 6-phosphogluconate dehydrogenase from Lactococcus lactis

引用

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 1998年第6-2期54卷 1422-1424页

作者： Tetaud, E Hall, DR Gourley, DG Leonard, GA Arkison, S Barrett, MP Hunter, WN Univ Dundee Dept Biochem Dundee DD1 4HN Scotland Joint Struct Biol Grp ESRF F-38043 Grenoble France Univ Glasgow Inst Biomed & Life Sci Dept Biochem Parasitol Glasgow G12 8QQ Lanark Scotland

6-Phosphogluconate dehydrogenase is one of the seven enzymes involved in the pentose phosphate pathway. Crystals of a mammalian and a protozoan enzyme have been obtained previously and structures determined. It is reported here that a bacterial fi-phosphogluconate dehydrogenase, from Lactococcus lactis, has been purified and used in crystallization trials. Large prisms suitable for a detailed structural analysis have been obtained and characterized as orthorhombic, space group F222, with a = 70.4, b = 105.7, c = 474.6 Angstrom. Diffraction has been observed to 2.2 Angstrom resolution using synchrotron radiation. Structural analysis, in combination with ongoing biochemical characterization, will assist the elucidation of the structure-activity relationships of this enzyme.

关键词：细菌蛋白质类/化学细菌蛋白质类/分离和提纯结晶结晶学 X线二聚作用乳乳球菌/酶学磷酸戊糖途径磷酸葡糖脱氢酶/化学磷酸葡糖脱氢酶/分离和提纯蛋白质构象重组融合蛋白质类/化学重组融合蛋白质类/分离和提纯

来源：评论

学校读者我要写书评

暂无评论

GENETICS OF BACTERIAL STRESS RESPONSE AND ITS APPLICATIONS

引用

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES 1992年第1期665卷 1-15页

作者： MATIN, A Department of Microbiology and Immunology Stanford University School of Medicine Stanford California 94305-5402

来源：评论

学校读者我要写书评

暂无评论

金黄色葡萄球菌蛋白A的纯化

引用

上海医学 1980年第4期 39-41页

作者：司马蕙兰上海市卫生防疫站

来源：评论

学校读者我要写书评

暂无评论

MECHANISM OF REVERSIBLE GLYCINE CLEAVAGE REACTION IN ARTHROBACTER-GLOBIFORMIS - FUNCTION OF LIPOIC ACID IN CLEAVAGE AND SYNTHESIS OF GLYCINE

引用

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 1976年第1期173卷 71-81页

作者： KOCHI, H KIKUCHI, G TOHOKU UNIV SCH MEDDEPT BIOCHEMSENDAI 980JAPAN

The hydrogen carrier protein (H-protein) from Arthrobacter globiformis was found to contain one lipoic acid residue per molecule. In the glycine cleavage reaction, H-protein could be replaced by lipoic acid and in glycine synthesis, by dihydrolipoic acid. Lipoamide was less effective than lipoic acid in substituting for H-protein. In glycine synthesis with dihydrolipoic acid, even tetrahydrofolate and T-protein, an enzyme catalyzing the tetrahydrofolate-dependent step of the reaction, were not required, and the whole process of glycine synthesis could proceed in the presence of only one enzyme, P-protein, a pyridoxal phosphate-requiring enzyme. In the glycine decarboxylation reaction with lipoic acid, the decarboxylation product seemed to be bound to lipoic acid, possibly in the SCH2 NH2 form, which was extractable with benzene. The suspected intermediate compound could also be formed nonenzymically from dihydrolipoic acid, formaldehyde and ammonia, and glycine was obtained when the benzene extract containing the suspected intermediate compound was incubated with P-protein and NaHCO3 . Several lines of evidence suggest that a thiol group of dihydrolipoic acid reacts first with formaldehyde to form a hemimercaptal or mercaptal to which ammonia is subsequently fixed; the binding of ammonia, however, appears to be unstable. Based on observations with free lipoic acids, the sequence of the T-protein-dependent reaction steps in the enzymic, reversible glycine cleavage reaction is discussed.

关键词：节杆菌属/药物作用节杆菌属/代谢细菌蛋白质类/分离和提纯细菌蛋白质类/代谢细菌蛋白质类/生理学甘氨酸/代谢动力学脂类/分析脂类/药理学

来源：评论

学校读者我要写书评

暂无评论

SEPARATION OF INNER AND OUTER MEMBRANES OF RHODOPSEUDOMONAS-SPHEROIDES

引用

PREPARATIVE BIOCHEMISTRY 1976年第1期6卷 61-79页

作者： DING, DH KAPLAN, S UNIV ILLINOIS DEPT MICROBIOL URBANA IL 61801 USA

The separation of the inner and outer membranes of R. sphaeroides was achieved through sucrose density gradient (20%, 40%, 60%, wt/wt) centrifugation. The upper fraction of the gradient, with a specific density 1.181 (g/cm3), is high in cytochrome and succinate dehydrogenase activities, low in lipopolysaccharides, and it is designated the inner membrane fraction. The bottom fraction of the gradient, with a specific density 1.240, is high in lipopolysaccharide and contains neither cytochrome nor succinate dehydrogenase activities. This fraction is the cell wall or outer membrane fraction. The intermediate band on the gradient is an unseparated fraction of inner and outer membrane fragments. This fraction has a specific density of 1.211 and represents less than 3% of the total crude envelope. Thin sections of the vesicles of the inner membrane fraction and those of the outer membrane provide morphological evidence for the identity of the individual membrane fractions. At least 22 protein bands are resolved by employing sodium dodecyl sulfate slab gel electrophoresis. Six bands are present only in the inner membrane and 2 bands are found exclusively in the outer membrane. Most of the remaining polypeptides are present in greater amounts in the inner membrane relative to the outer membrane fractions.

关键词：细菌蛋白质类/分离和提纯细胞分级分离/方法细胞膜/超微结构离心法梯密度/方法电泳聚丙烯酰氨凝胶显微镜检查电子红杆菌球形/超微结构分光光度法

来源：评论

学校读者我要写书评

暂无评论

APPLICATION OF IMAGE INTENSIFIER FOR AUTORADIOGRAPHY OF POLYACRILAMIDE GELS

引用

ANALYTICAL BIOCHEMISTRY 1975年第1期66卷 100-103页

作者： BRISGUNOV, V REBENTISH, B GORDON, I DEBABOV, V ATOM ENERGY INST GENET & SELECTION IND MICROORG INSTMOSCOWUSSR

The new technique for autoradiography of polyacrilamide gels is described which utilizes image intensifier. Modified procedure reduces the exposure periods by factor of approximately 10 3 -fold. The special advantage ... 详细信息

关键词：放射自显影术/方法细菌蛋白质类/分离和提纯碳放射性同位素大肠杆菌噬菌体/分析光密度测定法电泳圆盘/仪器和设备大肠杆菌/分析图像增强蛋白质类/分离和提纯病毒蛋白质类/分离和提纯

来源：评论

学校读者我要写书评

暂无评论

MEMBRANE-BOUND SUBSTRATE RECOGNITION COMPONENTS OF DICARBOXYLATE TRANSPORT-SYSTEM IN ESCHERICHIA-COLI

引用

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1975年第1期63卷 278-285页

作者： LO, TCY SANWAL, BD UNIV WESTERN ONTARIO DEPT BIOCHEMLONDON N6A 5C1ONTARIOCANADA

Membranes isolated from Escherichia coli and solubilized with Lubrol 17A-10 contain two proteins, designated SBP 1 and SBP 2 which can be separated and purified on aspartate-coupled Sepharose 4B. Both of the proteins bind succinate, malate and fumarate but not aspartate, malonate, tartarate and acetate. The dissociation constants of SBP 1 and SBP 2 for succinate are 23 μM and 2.3 μM, respectively. The binding of succinate to these components is inhibited by sulfhydryl reagents. A mutant of Escherichia coli (dct A ) which is unable to transport succinate and other dicarboxylic acids has very small amounts of the membrane bound SBP 2 protein. Genetic and biochemical evidence strongly suggests that SBP 1 and SBP 2 cooperate in some unknown way in the transport of succinate in vivo .

关键词：细菌蛋白质类/分离和提纯细菌蛋白质类/代谢结合部位结合竞争性生物转运主动细胞膜/代谢色谱法亲和洗涤剂二羧酸类/代谢大肠杆菌/代谢动力学突变蛋白质结合受体药物溶解度种特异性

来源：评论

学校读者我要写书评

暂无评论

CHARACTERIZATION OF A NOVEL, LOW-MOLECULAR-WEIGHT DNA-BINDING PROTEIN FROM ESCHERICHIA-COLI

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1975年第9期72卷 3428-3432页

作者： ROUVIEREYANIV, J GROS, F INST PASTEUR DEPT BIOL MOLEC 25 RUE DR ROUX 75015 PARIS FRANCE

A low-molecular-weight (7000), heat-stable protein--HU--that stimulates transcription of bacteriophage lambda DNA by E. coli RNA polymerase was purified from E. coli extracts using affinity chromatography on DNA-cellulose. HU binds to native DNA, resulting in an apparent thickening of the DNA chains as revealed by electron microscopy. Contrary to DNA unwinding proteins, it causes no destabilization of the double helix. HU differs from previously described transcription factors (H1, D, etc.) and from the low-molecular-weight omega subunit of the RNA polymerase. By its amino-acid composition and characteristics, HU displays an interesting resemblance to some eukaryotic histones, such as H2B and H1.

关键词：氨基酸类/分析细菌蛋白质类/分离和提纯细菌蛋白质类/代谢结合部位大肠杆菌噬菌体 DNA 细菌/代谢 DNA 病毒/代谢大肠杆菌/代谢热温度分子量转录遗传/药物作用

来源：评论

学校读者我要写书评

暂无评论

INTERACTION OF ESCHERICHIA-COLI DNAB AND DNAC(D) GENE PRODUCTS INVITRO

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1975年第3期72卷 921-925页

作者： WICKNER, S HURWITZ, J ALBERT EINSTEIN COLL MED DEPT DEV BIOL & CANCDIV BIOL SCIBRONXNY 10461 NCI VIRAL CARCINOGENESIS BRANCHBETHESDAMD 20014

Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.

关键词：腺苷三磷酸/代谢细菌蛋白质类/分离和提纯细菌蛋白质类/代谢色谱法凝胶 DNA复制 DNA 细菌/代谢大肠杆菌/代谢乙基马来酰亚胺/药理学基因

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：