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The effect of growth hormone-releasing peptide-2 (KP102) administration on plasma insulin-like growth factor (IGF)-1 and IGF-binding proteins in Holstein steers on different planes of nutrition

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DOMESTIC ANIMAL ENDOCRINOLOGY 2000年第3期18卷 293-308页

作者： Lee, HG Vega, RA Phung, LT Matsunaga, N Kuwayama, H Hidari, H Obihiro Univ Agr & Vet Med Dept Anim Sci Lab Anim Prod Obihiro Hokkaido 0808555 Japan

This study was conducted to investigate the nutrition-dependent changes in insulin-like growth factor (IGF)-1 and IGF-binding proteins (IGFBPs) with growth hormone releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2;GHRP-2 or KP102) treatment in growing Holstein steers. Eight 13 month-old Holstein steers were grouped on two levels of feed intake (high intake (HI);2.43% body weight or low intake (LI);1.22%) and each group was daily injected with KP102 (12.5 mu g/kg body weight/day) or saline solution into the jugular vein during 6-day period. The concentration of plasma GH showed an increase after an i.v. bolus injection of KP102 on Day 1 and Day 6 in both the LI and HI groups. Plasma IGF-1 began to increase 10 hr following an i.v. bolus injection of KP102, but this was only observed in the HI group (P < 0.05). Also, the plasma IGF-1 in the HI group with daily injections was significantly greater than the LI group from Day 1 of KP102 administration (P < 0.05). It reached maximum values of 125.1 +/- 7.6 ng/ml after Day 2, and returned to pre-injection levels after Day 4, however, no change in plasma IGF-1 was observed in LI with administration of KP102. During 6 days of treatment, plasma 38-43 kDa IGFBP-3 and 24 kDa IGFBP-4 were significantly higher in KP102 treated steers but only in the HI group (P < 0.05). Plasma 34 kDa IGFBP-2 decreased in the HI group and did not show any change following an injection of KP102. In conclusion, the effect of stimulated endogenous GH with KP102 administration increased plasma IGF-1, 38-43 kDa IGFBP-3 and 24 kDa IGFBP-4 levels in the HI group of growing Holstein steers, but not in the LI one. Thus, we strongly believe that the plasma IGF-1 and IGFBPs response to KP102 treatment is modulated by the nutritional status of growing Holstein steers and the increased plasma IGF-1 concentration with KP102 treatment may be regulated by plasma 38-43 kDa IGFBP-3 and 24 kDa IGFBP-4 in Holstein steers. (C) 2000 Elsevier Science Inc. All r

关键词：动物营养生理学曲线下面积血尿素氮印迹法蛋白质/兽医学牛/生理学进食/生理学生长激素/血液生长激素/生理学生长激素释放激素/投药和剂量生长激素释放激素/药理学激素类/投药和剂量激素类/药理学胰岛素/血液胰岛素样生长因子结合蛋白质类/血液胰岛素样生长因子结合蛋白质类/分泌胰岛素样生长因子Ⅰ/分析胰岛素样生长因子ⅰ/分泌线性模型氮/分析营养状况寡肽类/投药和剂量寡肽类/药理学放射免疫测定/兽医学动物男(雄)性

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mRNA of insulin-like growth factor (IGF) quantification and presence of IGF binding proteins, and receptors for growth hormone, IGF-I and insulin, determined by reverse transcribed polymerase chain reaction, in the liver of growing and mature male cattle

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DOMESTIC ANIMAL ENDOCRINOLOGY 2000年第3期19卷 191-208页

作者： Cordano, P Hammon, HM Morel, C Zurbriggen, A Blum, JW Univ Bern Fac Med Vet Div Nutr Pathol CH-3012 Bern Switzerland Univ Bern Fac Med Vet Inst Anim Nuerol CH-3012 Bern Switzerland

Plasma insulin-like growth factor-I (IGF-I) concentrations were related to hepatic levels of IGF-I mRNA measured by competitive reverse transcription polymerase chain reaction (PCR) (RT-PCR) in neonatal (8 d old) calves, veal calves, fattened castrated bulls and mature intact bulls. Furthermore, the presence of mRNAs of IGF-II and of receptors for IGF-I (ICF-IR), growth hormone (GHR) and insulin (IR), as well as mRNAs of IGF binding proteins (IGFBP-1, -2 and -3) were assessed by RT-PCR. Hepatic ICF-I mRNA levels and plasma IGF-I concentrations in veal calves, fattened castrated bulls and in intact bulls were 4 to 8 times higher than in 8-d old calves and were 2 to 3 times higher in calves fed colostrum than in calves fed only milk replacer. Hepatic IGF-I mRNA concentrations were closely correlated (r = 0.92) with plasma IGF-I concentrations, suggesting that hepatic IGF-I production largely determines plasma IGF-I levels. The presence of ICF II, IGF-IR, GHR, IR and IGFBP-1, -2 and -3 mRNA was confirmed in the liver of 8-d old calves and older cattle as well, and among newborn calves their presence was independent of differences in nutrition. In conclusion, the major hepatic components of the GH-IGF axis were present in neonatal calves, but the IGF-I expression and therefore also plasma IGF-I levels were relatively low. (C) 2000 Elsevier Science Inc. All rights reserved.

关键词：氨基酸序列动物新生碱基序列牛/遗传学牛/生长和发育牛/生理学初乳/生理学 DNA/化学 DNA引物/化学电泳琼脂凝胶/兽医学胰岛素样生长因子结合蛋白质类/分析胰岛素样生长因子结合蛋白质类/遗传学胰岛素样生长因子Ⅰ/分析胰岛素样生长因子Ⅰ/遗传学胰岛素样生长因子ⅰ/分泌胰岛素样生长因子Ⅱ/分析胰岛素样生长因子Ⅱ/遗传学肝/化学肝/生理学分子序列数据 RNA 信使/分析 RNA 信使/化学放射免疫测定/兽医学受体胰岛素/分析受体胰岛素/遗传学受体生长调节素/分析受体生长调节素/遗传学受体促生长素/分析受体促生长素/遗传学逆转录聚合酶链反应/兽医学动物女(雌)性男(雄)性妊娠

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Route of estrogen administration helps to determine growth hormone (GH) replacement dose in GH-deficient adults

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JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM 1999年第11期84卷 3956-3960页

作者： Cook, DM Ludlam, WH Cook, MB Oregon Hlth & Sci Univ Portland OR 97201 USA

We prospectively studied two groups of GH-deficient patients during GH therapy based upon exposure of the liver to elevated (oral estrogen) or not elevated (endogenous or transdermal) sources of estrogen. We wondered whether higher concentrations of estrogen at the liver level (oral estrogen) might inhibit insulin-like growth factor I (IGF-I) secretion and alter exogenous GH requirements. In this study we compared GH replacement requirements in these two groups of women as well as with GH-treated adult hypopituitary males. The final GH dose was based upon maintenance IGF-I levels in the mid- to high normal range adjusted for age and sex or symptom tolerance. Each group [women taking oral estrogen (n = 12), women not taking oral estrogen (n = 13), and men (n = 12)] was similar in age and final IGF-I concentration. Women taking oral estrogen required 10.6 +/- 0.7 mu g/*** or 867 +/- 45 mu g/day GH, women not taking oral estrogen required 5.0 +/- 0.7 mu g/*** or 424 +/- 57 mu g/day, and men required 4.1 +/- 0.6 mu g/*** of 376 +/- 49 mu g/day to achieve similar IGF-I concentrations. GH requirements in men were not different from those in women not taking oral estrogen, but the GH requirements in both groups were significantly different from GH requirements in women taking oral estrogen. These observations may be useful in anticipating appropriate starting and final doses of GH in adult hypopituitary patients.

关键词：投药皮肤投药口服雌二醇/投药和剂量激素替代疗法人生长激素/投药和剂量人生长激素/缺乏人生长激素/治疗应用胰岛素样生长因子ⅰ/分泌前瞻性研究成年人女(雌)性人类男(雄)性中年人

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