检索结果-内蒙古大学图书馆

中医杂志 2006年第8期47卷 613-615页

作者：姚晖张继平陈芝喜李志强佛山市第二人民医院药剂科科教科广东省佛山市卫国路78号528000 广州中医药大学核医学实验室

目的:探讨补阳还五汤及黄芪体外对家兔血小板活化因子(PAF)受体活性的作用,阐明黄芪在该方中的配伍机制。方法:将动物分为补阳还五汤全方组、活血组和补气组,采用放射配基受体结合实验,以家兔血小板PAF受体特异结合抑制率为评价指标,探... 详细信息

目的:探讨补阳还五汤及黄芪体外对家兔血小板活化因子(PAF)受体活性的作用,阐明黄芪在该方中的配伍机制。方法:将动物分为补阳还五汤全方组、活血组和补气组,采用放射配基受体结合实验,以家兔血小板PAF受体特异结合抑制率为评价指标,探讨3组体外对家兔血小板PAF受体活性的影响。结果:3组均能抑制3H-PAF与家兔血小板PAF受体的特异结合,对PAF受体特异结合抑制程度由大到小依次为活血组、全方组、补气组,各组之间抑制率差异均有显著性(P<0.01)。结论:单味黄芪(120g)对家兔血小板PAF受体与3H-PAF的特异结合有一定的抑制作用,但黄芪与方中活血药配伍后,能使后者拮抗血小板PAF受体的作用明显减弱,使全方拮抗血小板PAF受体的作用缓和,防止大量活血药攻伐太过,体现了补阳还五汤“祛瘀而不伤正”的配伍机制。

关键词：补阳还五汤/药理学血小板活化因子/代谢

来源：评论

学校读者我要写书评

暂无评论

Inflammatory platelet-activating factor-like phospholipids in oxidized low density lipoproteins are fragmented alkyl phosphatidylcholines

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 1999年第40期274卷 28395-28404页

作者： Marathe, GK Davies, SS Harrison, KA Silva, AR Murphy, RC Castro-Faria-Neto, H Prescott, SM Zimmerman, GA McIntyre, TM Univ Utah Dept Pathol Salt Lake City UT 84112 USA Univ Utah Dept Internal Med Salt Lake City UT 84112 USA Univ Utah Huntsman Canc Inst Salt Lake City UT 84112 USA Natl Jewish Med & Res Ctr Dept Pediat Denver CO 80206 USA Fiocruz MS Dept Fisiol & Farmacodinam IOC BR-21045900 Rio De Janeiro Brazil

Oxidation of human low density lipoprotein (LDL) generates proinflammatory mediators and underlies early events in atherogenesis. We identified mediators in oxidized LDL that induced an inflammatory reaction in vivo, and activated polymorphonuclear leukocytes and cells ectopically expressing human platelet-activating factor (PAF) receptors, Oxidation of a synthetic phosphatidylcholine showed that an sn-l ether bond confers an 800-fold increase in potency. This suggests that rare ether-linked phospholipids in LDL are the likely source of PAF-like activity in oxidized LDL, Accordingly, treatment of oxidized LDL with phospholipase A, greatly reduced phospholipid mass, but did not decrease its PAF-like activity. Tandem mass spectrometry identified traces of PAF, and more abundant levels of 1-O-hexadecyl-2-(butanoyl or butenoyl)-sn-glycero-3-phosphocholines (C-4-PAF analogs) in oxidized LDL that comigrated with PAF-like activity. Synthesis showed that either C-4-PAF was just 10-fold less potent than PAF as a PAF receptor ligand and agonist. Quantitation by gas chromatography-mass spectrometry of pentafluorobenzoyl derivatives shows the C-4-PAF analogs were 100-fold more abundant in oxidized LDL than PAF, Oxidation of synthetic alkyl arachidonoyl phosphatidylcholine generated these C-4-PAFs in abundance. These results show that quite minor constituents of the LDL phosphatidylcholine pool are the exclusive precursors for PAF-like bioactivity in oxidized LDL.

关键词：色谱法高压液相炎症介导素类/化学炎症介导素类/代谢脂蛋白类 LDL/代谢质谱分析法氧化还原磷脂酰胆碱类/化学磷脂酰胆碱类/代谢血小板活化因子/化学血小板活化因子/代谢重组蛋白质类/化学重组蛋白质类/代谢人类

来源：评论

学校读者我要写书评

暂无评论

Platelet-activating factor induces an imbalance between matrix metalloproteinase-1 and tissue inhibitor of metalloproteinases-1 expression in human uterine cervical fibroblasts

引用

BIOLOGY OF REPRODUCTION 2000年第3期62卷 540-546页

作者： Sugano, T Nasu, K Narahara, H Kawano, Y Nishida, Y Miyakawa, I Oita Med Univ Dept Obstet & Gynecol Oita 8795593 Japan

Platelet-activating factor (PAF) is involved in such reproductive processes as parturition. We investigated the effect of PAF on the expression of matrix metalloproteinase-1 (MMP-1) and that of tissue inhibitor of metalloproteinases-1 (TIMP-1) in human uterine cervical fibroblasts. Uterine cervical tissue was obtained from patients who underwent cesarean section at term. Collagenase-dispersed fibroblasts were cultured and used in the experiments. PAF receptor was identified in the uterine cervical fibroblasts by use of reverse transcription-polymerase chain reaction and Southern blot analysis. Northern blot analysis showed that PAF increased the expression of MMP-1 mRNA in a time-dependent manner, whereas expression of TIMP-1 mRNA was not affected by PAF. Concentration of MMP-1 protein in the PAF-treated culture media significantly exceeded that in control cultures. The PAF-induced production of MMP-1 protein was abolished by treatment with WEB 2170, a specific PAE receptor antagonist. Results suggest that PAF may accelerate collagenolysis in the human uterine cervix by inducing an imbalance in the activity between MMP-1 and TIMP-1, thus contributing to the cervical ripening during parturition.

关键词：细胞培养的子宫颈/细胞学子宫颈/药物作用子宫颈/代谢成纤维细胞/药物作用成纤维细胞/代谢基因表达调控基质金属蛋白酶1/遗传学基质金属蛋白酶1/代谢血小板活化因子/代谢血小板活化因子/药理学血小板膜糖蛋白类/遗传学前胶原/遗传学 RNA 信使受体细胞表面受体 G-蛋白偶联金属蛋白酶1组织抑制剂/遗传学金属蛋白酶1组织抑制剂/代谢成年人女(雌)性人类妊娠

来源：评论

学校读者我要写书评

暂无评论

Motility induced by human immunodeficiency virus-1 Tat on Kaposi's sarcoma cells requires platelet-activating factor synthesis

引用

AMERICAN JOURNAL OF PATHOLOGY 1999年第5期155卷 1731-1739页

作者： Biancone, L Cantaluppi, V Boccellino, M Bussolati, B Del Sorbo, L Conaldi, PG Albini, A Toniolo, A Camussi, G Univ Turin Dipartimento Med Interna Cattedra Nefrol Turin Italy Ist Nazl Ric Canc I-16132 Genoa Italy Univ Insubria Dipartimento Sci Clin & Biol Cattedra Microbiol Varese Italy

In the present study, we evaluated whether motility of Kaposi's sarcoma (KS) spindle cells induced by HIV-1 Tat protein is dependent on the synthesis of platelet-activating factor (PAF). The results obtained indicate that Tat induced a dose-dependent synthesis of PAF from KS cells at a concentration as low as 0.1 ng/ml. PAF production started rapidly after Tat stimulation, peaking at 30 minutes and declining thereafter. Tat-induced cell migration was also a rapid event starting at 30 minutes. The motility was abrogated by addition of a panel of chemically unrelated PAF receptor antagonists (WEB 2170, CV 3988, CV 6209, and BN 52021), suggesting that the synthesized PAF mediates the motogenic effect of Tat. This effect was also present on cells plated on a type-I collagen-, fibronectin-, or basement membrane extract-coated surface. Expression of PAF receptor-specific mRNA was detected in KS cells. In addition, examination of the cytoskeletal organization showed that Tat-mediated KS cell redistribution of actin filaments and shape change was also inhibited by a PAF receptor antagonist. Moreover, PAF receptor blockade prevented the up-regulation of pi integrin and the down-regulation of alpha v beta 3 observed after stimulation of KS cells with Tat In conclusion, the results of the present study indicate that Tat-induced PAF synthesis plays a critical role in triggering the events involved in motility of KS cells.

关键词：吖庚因类/药理学细胞运动/药物作用二萜类剂量效应关系药物纤维蛋白溶解药/药理学基因产物 tat/代谢基因产物 tat/药理学银杏苦内酯类 HIV-1 内酯类/药理学磷脂醚类/药理学血小板活化因子/代谢血小板聚集抑制剂/药理学血小板膜糖蛋白类/拮抗剂和抑制剂血小板膜糖蛋白类/代谢吡啶鎓化合物/药理学受体细胞表面受体 G-蛋白偶联肉瘤卡波西/代谢肉瘤卡波西/病理学三唑类/药理学肿瘤细胞培养的 tat基因产物人免疫缺陷病毒人类

来源：评论

学校读者我要写书评

暂无评论

Platelet-activating factor acetylhydrolase activity in cerebrospinal fluid of children with acute systemic or neurological illness

引用

ANNALS OF NEUROLOGY 2002年第6期51卷 760-763页

作者： Chang, HW Kwon, S Kim, H Lee, K Kim, M Moon, T Baek, S Kyungpook Natl Univ Hosp Dept Pediat Joong Gu 700721 Daegu South Korea Yeungnam Univ Coll Pharm Gyongsan South Korea Yeungnam Univ Coll Med Taegu South Korea

Platelet-activating factor acetylhydrolase was analyzed in cerebrospinal fluid samples taken from children with a variety of neurological conditions (85 patients;mean age, 3.8 years) to determine it is involved in the defense mechanism against the toxic effect of inflammatory mediators in the central nervous system. A significant increase in cerebrospinal fluid activity was seen in the patients with meningitis and acute febrile illness in comparison with the control subjects. The activity was also significantly higher in the patients with meningitis than in the patients with inflammatory neurological diseases. In addition, the biochemical profile of cerebrospinal fluid platelet-activating factor acetylhydrolase was different from other known acetylhydrolases. These findings suggest that cerebrospinal fluid platelet-activating factor acetylhydrolase activity may be a sensitive marker of the host response to central nervous system infections.

关键词： 1-烷基-2-乙酰甘油磷酸胆碱酯酶年龄因素中枢神经系统疾病/脑脊髓液中枢神经系统疾病/酶学磷脂酶A/脑脊髓液磷脂酶A/化学血小板活化因子/代谢青少年儿童儿童学龄前女(雌)性人类婴儿新生男(雄)性

来源：评论

学校读者我要写书评

暂无评论

Vasoactive side effects of intravenous immunoglobulin preparations in a rat model and their treatment with recombinant platelet-activating factor acetylhydrolase

引用

BLOOD 2000年第5期95卷 1856-1861页

作者： Bleeker, WK Teeling, JL Verhoeven, AJ Rigter, GMM Agterberg, J Tool, ATJ Koenderman, AHL Kuijpers, TW Hack, CE CLB Dept PDH NL-1066 CX Amsterdam Netherlands

Previously, we observed in a rat model that intravenous administration of intramuscular immunoglobulin preparations induced a long-lasting hypotension, which appeared to be associated with the presence of IgG polymers and dimers in the preparations, but unrelated to complement activation. We found evidence that this hypotensive response is mediated by platelet-activating factor (PAF) produced by macrophages. In this study, we compared the vasoactive effects of 16 intravenous immunoglobulin (IVIG) products from 10 different manufacturers, in anesthetized rats, Eight of the IVIG preparations showed no hypotensive effects (less than 15% decrease), whereas the other 8 had relatively strong effects (15%-50% decrease). The hypotensive effects correlated with the IgG dimer content of the preparations. Pretreatment of the rats with recombinant PAF acetylhydrolase completely prevented the hypotensive reaction on IVIG infusion, and administration after the onset of hypotension resulted in normalization of the blood pressure. We also observed PAF production on in vitro incubation of human neutrophils with IVIG, which could be blocked by anti-Fc gamma receptor antibodies. This indicates that induction of PAF generation may also occur in a human system. Our findings support the hypothesis that the clinical side effects of IVIG in patients may be caused by macrophage and neutrophil activation through interaction of IgG dimers with Fc gamma receptors, Because phagocyte activation may also lead to the release of other inflammatory mediators, recombinant PAF acetylhydrolase (rPAF-AH) provides a useful tool to determine whether PAF plays a role in the clinical side effects of IVIG. If so, rPAF-AH can be used for the treatment of those adverse reactions. (C) 2000 by The American Society of Hematology.

关键词： 1-烷基-2-乙酰甘油磷酸胆碱酯酶二聚作用药物稳定性低血压/病因学低血压/预防和控制免疫球蛋白G/药理学免疫球蛋白G/毒性免疫球蛋白类静脉内/毒性巨噬细胞/药物作用巨噬细胞/分泌中性白细胞/药物作用中性白细胞/代谢磷脂酶A/遗传学磷脂酶A/药理学磷脂酶A/治疗应用血小板活化因子/代谢血小板活化因子/分泌大鼠 Wistar 受体 IgG/拮抗剂和抑制剂受体 IgG/生理学重组融合蛋白质类/药理学重组融合蛋白质类/治疗应用动物女(雌)性人类大鼠

来源：评论

学校读者我要写书评

暂无评论

Role of platelet-activating factor in cardiovascular pathophysiology

引用

PHYSIOLOGICAL REVIEWS 2000年第4期80卷 1669-1699页

作者： Montrucchio, G Alloatti, G Camussi, G Univ Turin Lab Immunopatol Renale Turin Italy Univ Turin Dipartimento Med Interna Turin Italy Univ Turin Dipartimento Biol Anim & Uomo Turin Italy Univ Turin Ist Nazl Fis Mat Turin Italy

Platelet-activating factor (PAF) is a phospholipid mediator that belongs to a family of biologically active, structurally related alkyl phosphoglycerides. PAF acts via a specific receptor that is coupled with a G protein, which activates a phosphatidylinositol-specific phospholipase C. In. this review we focus on the aspects that are more relevant for the cell biology of the cardiovascular system. The in vitro studies provided evidence for a role of PAF both as intercellular and intracellular messenger involved in cell-to-cell communication. In the cardiovascular system, PAF may have a role in embryogenesis because it stimulates endothelial cell migration and angiogenesis and may affect cardiac function because it exhibits mechanical and electrophysiological actions on cardiomyocytes. Moreover, PAF may contribute to modulation of blood pressure mainly by affecting the renal vascular circulation. In pathological conditions, PAF has been involved in the hypotension and cardiac dysfunctions occurring in various cardiovascular stress situations such as cardiac anaphylaxis and hemorrhagic, traumatic, and septic shock syndromes. In addition, experimental studies indicate that PAF has a critical role in the development of myocardial ischemia-reperfusion injury. Indeed, PAF cooperates in the recruitment of leukocytes in inflamed tissue by promoting adhesion to the endothelium and extravascular transmigration of leukocytes. The finding that human heart can produce PAF, expresses PAF receptor, and is sensitive to the negative inotropic action of PAF suggests that this mediator may have a role also in human cardiovascular pathophysiology.

关键词：过敏反应/代谢心血管疾病/代谢心血管疾病/病理生理学细胞培养的内皮血管/药物作用内皮血管/免疫学内皮血管/代谢心脏/药物作用心脏/病理生理学血流动力学/药物作用微循环/药物作用心肌/细胞学心肌/代谢新生血管化病理性/代谢新生血管化生理性/药物作用器官特异性/药物作用血小板活化因子/代谢血小板活化因子/药理学血小板膜糖蛋白类/代谢受体细胞表面受体 G-蛋白偶联再灌注损伤/代谢信号传导/药物作用动物人类

来源：评论

学校读者我要写书评

暂无评论

Plasma platelet-activating factor acetylhydrolase activity is not associated with premature coronary atherosclerosis

引用

AMERICAN JOURNAL OF CARDIOLOGY 1999年第1期83卷 109-+页

作者： Shohet, RV Anwar, A Johnston, JM Cohen, JC Univ Texas SW Med Ctr Ctr Human Nutr Dallas TX 75235 USA Univ Texas SW Med Ctr Dept Med Dallas TX 75235 USA Univ Texas SW Med Ctr Dept Biochem Dallas TX 75235 USA Baylor Univ Med Ctr Dallas TX USA

Platelet-activating factor acetylhydrolase activity in plasma was compared between 72 subjects with angiographically normal coronary arteries and matched controls with clinically significant obstruction. No difference was seen, and we conclude that variation in plasma platelet-activating factor acetylhydrolase activity is not a risk factor for coronary artery disease.

关键词： 1-烷基-2-乙酰甘油磷酸胆碱酯酶冠状血管造影术磷脂酶A/血液血小板活化因子/代谢危险因素成年人女(雌)性人类男(雄)性中年人

来源：评论

学校读者我要写书评

暂无评论

慢性肺心病患者血小板活化状态的检测及临床意义

引用

中国医师杂志 2010年第2期12卷 262-264页

作者：张志强马海英河南省新乡医学院一附院呼吸科河南省卫辉453100

目的探讨慢性肺心病患者血小板膜糖蛋白CD62p、PAC-1的变化及其临床意义。方法检测40例慢性肺心病患者外周血中血小板CD62p、PAC-1的表达水平，并与30例健康对照者比较。用超声心动图RVPEP／AT值估测肺心病患者肺动脉压。结果肺心病组CD... 详细信息

目的探讨慢性肺心病患者血小板膜糖蛋白CD62p、PAC-1的变化及其临床意义。方法检测40例慢性肺心病患者外周血中血小板CD62p、PAC-1的表达水平，并与30例健康对照者比较。用超声心动图RVPEP／AT值估测肺心病患者肺动脉压。结果肺心病组CD62p、PAC-1的表达均明显高于对照组（P〈0．01），并与肺心病组RVPEP／AT值呈正相关。结论慢性肺心病患者血小板明显活化，其活化程度与肺动脉高压关系密切。

关键词：肺心病/代谢血小板活化血小板活化因子/代谢

来源：评论

学校读者我要写书评

暂无评论

过敏性哮喘豚鼠血小板激活因子的变化及川芎嗪的影响

引用

中华结核和呼吸杂志 1993年第3期16卷 152-152页

作者：于化鹏李平升府军第一军医大学珠江医院呼吸科第二军医大学长海医院呼吸科南京八一医院呼吸科

血小板激活因子(PAF)最近10余年被认识的重要炎性介质,与气道的炎症反应及滑肌痉挛关系密切。为探讨PAF在过敏性哮喘中的作用及川芎嗪对PAF的影响,我们采用生物定量法和放射免疫方法观察了40只豚鼠血浆溶血血小板激活因子(lyso—PAF)、肺泡

关键词：川芎嗪/药理学哮喘/病理生理学哮喘/中药疗法血小板活化因子/代谢动物豚鼠

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：