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江西医学院学报 1986年第3期 28-30页

作者：高摄渊袁龙庆曾司鲁王桂秀江西医学院组织胚胎学教研室江西医学院人体解剖学教研室

应用电镜观察了人类成体相对正常和二月胎儿的脑内血管,发现前者进入脑内的血管有微动脉、中间微动脉及毛细血管;后者脑内仅见毛细血管。脑内毛细血管衬以单层无孔连续性内皮,内皮细胞向管腔面和基膜面发出突起,在细胞间连接处有突向管... 详细信息

应用电镜观察了人类成体相对正常和二月胎儿的脑内血管,发现前者进入脑内的血管有微动脉、中间微动脉及毛细血管;后者脑内仅见毛细血管。脑内毛细血管衬以单层无孔连续性内皮,内皮细胞向管腔面和基膜面发出突起,在细胞间连接处有突向管腔的边缘褶。脑肿瘤患者及胎脑血管中内皮细胞向管腔的突起长而分枝,呈皱襞状。并对毛细血管内皮细胞的细微结构进行了观察描述。

关键词：血管/超微结构血管/胚胎学血脑屏障星状细胞瘤/血液供给人类

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墨汁灌注和血管铸型方法检测山羊胫骨血管应用性能的不同

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中国临床康复 2006年第21期10卷 94-96,i0005页

作者：唐光辉裴国献王兴海陈滨曾宪利程文俊任义军魏宽海南方医科大学南方医院创伤骨科中心广东省广州市510515 南方医科大学人体解剖学实验室广东省广州市510515

目的:比较血管墨汁灌注和甲基丙烯酸甲酯血管铸型两种方法在山羊胫骨血管检测中的优缺点,为组织工程骨血管化检测寻找一种直观可行的方法。方法:实验于2004-05/2005-07在南方医科大学南方医院创伤骨科骨组织工程实验室完成。①12只正常... 详细信息

目的:比较血管墨汁灌注和甲基丙烯酸甲酯血管铸型两种方法在山羊胫骨血管检测中的优缺点,为组织工程骨血管化检测寻找一种直观可行的方法。方法:实验于2004-05/2005-07在南方医科大学南方医院创伤骨科骨组织工程实验室完成。①12只正常中国青山羊随机分为墨汁灌注组,甲基丙烯酸甲酯血管铸型灌注组,每组6只。②墨汁灌注组采用中华墨汁、甲醛及生理盐水体积比为3∶1∶6的复合墨汁,行双侧股动脉插管,经血管冲洗后进行灌注,低温放置24h后取下胫骨标本行进一步处理,甲基丙烯酸甲酯血管铸型灌注组,每只山羊均行双侧后肢股动脉插管,血管彻底冲洗后将预聚后的甲基丙烯酸甲酯灌注,低温放置24～28h后取下胫骨标本行下一步处理。③灌注后两组标本分别采用大体解剖,未脱钙骨磨片和脱钙后组织切片等方法观察青山羊胫骨血管的走行与分布。结果:①山羊胫骨经墨汁灌注后大体解剖、未脱钙骨磨片和脱钙骨组织切片观察可清楚显示骨膜、骨皮质和骨髓腔血管;可以观察骨组织的显微结构,但不便于直观地观察胫骨与周围血管的空间立体结构。②血管铸型后的山羊胫骨制作的大体解剖标本,在胫骨中段制作2cm×1cm骨窗,可见髓腔内的铸型血管,骨皮质横、纵切面上可见微细的铸型血管断面;可以直观的观察山羊胫骨与周围血管的空间立体构筑,显示胫骨营养血管的分布、走行,未脱钙磨片能够清楚显示骨皮质血管网,扫描电镜可以观察到哈佛氏管内血管。结论:血管墨汁灌注适合用于微血管检测,便于染色后同时观察骨组织的显微结构;而血管铸型标本则适合大体观察血管的空间立体构筑,较为直观,两者在组织工程骨血管化检测中有不同的应用价值。

关键词：组织工程血管/解剖学和组织学血管/超微结构山羊胫骨

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培养人血管内皮细胞冷冻复型的电镜观察

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中华病理学杂志 1992年第5期21卷 271-271,328页

作者：杨向红陈铁镇中国医科大学实验病理学研究室

冷冻复型技术是细胞生物学和分子生物学研究的重要方法,其特点是能够显示生物膜的内部形态结构。目前应用范围已较广泛,本实验应用此技术对培养人血管内皮细胞的微细结构进行了观察。

关键词：内皮血管/超微结构

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Pathogenesis of classical swine fever:: Renal haemorrhages and erythrodiapedesis

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JOURNAL OF COMPARATIVE PATHOLOGY 2000年第1期123卷 47-54页

作者： Gómez-Villamandos, JC Ruiz-Villamor, E Bautista, MJ Quezada, M Sánchez, CP Salguero, FJ Sierra, MA Univ Cardoba Fac Vet Dept Pathol Anat Cordoba 14014 Spain Univ Concepcion Dept Patol & Med Prevent Chillan Chile INIA Ctr Invest Sanidad Anim Madrid Spain

Thirty pigs were inoculated with a virulent isolate (Quillota strain) of classical swine fever (hog cholera) virus to establish the chronological occurrence of lesions in the kidney and to determine the mechanism responsible for renal haemorrhages. The study included the use of histopathological, ultrastructural, immunohistochemical (detection of viral antigen gp55, MAC387, lambda chains, CD3 and Clq) and morphometrical techniques (vascular area). Renal interstitial oedema and haemorrhages were detected from 7 da's post-inoculation (dpi), associated with a slight interstitial mononuclear infiltrate and evidence of viral infection in macrophages and fibroblasts, and in a small proportion of lymphocytes. Viral infection was not detected in capillary endothelial cells. An intense mononuclear infiltrate, with B cells, T cells and small numbers of macrophages, was detected from 10 dpi. In the final phase of the experiment (14 dpi, slight proliferation and degranulation of mast cells were observed. Increased expression of the Clq component of complement was also detected. A significant increase in vascular area was observed from 7 dpi. These results suggest that haemorrhages observed in the kidneys of pigs inoculated with the Quillota strain resulted from erythrodiapedesis and increased vascular permeability, probably aggravated by mast cell degranulation in the final stage of the experiment. The results suggested that mast cell degranulation was linked to activation of the complement system. (C) 2000 Harcourt Publishers Ltd.

关键词：抗原病毒/分析生物学标记/分析毛细血管/代谢毛细血管/超微结构毛细血管/病毒学毛细血管脆性/生理学毛细血管通透性/生理学细胞脱颗粒/生理学细胞运动/生理学猪瘟/病因学猪瘟/免疫学猪瘟/病理学猪瘟病毒/免疫学胞质小泡/生理学胞质小泡/超微结构水肿/病因学水肿/病理学内皮血管/代谢内皮血管/超微结构内皮血管/病毒学红细胞/细胞学红细胞/生理学出血/病因学出血/病理学肾疾病/病因学肾疾病/病理学肥大细胞/生理学肥大细胞/超微结构猪动物女(雌)性男(雄)性

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Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae

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JOURNAL OF BIOLOGICAL CHEMISTRY 2000年第3期275卷 2057-2062页

作者： Koziak, K Kaczmarek, E Kittel, A Sévigny, J Blusztajn, JK Esch, JSA Imai, M Guckelberger, O Goepfert, C Qawi, I Robson, SC Harvard Univ Sch Med Beth Israel Deaconess Med Ctr Dept MedCtr Immunobiol Boston MA 02215 USA Hungarian Acad Sci H-1450 Budapest Hungary Boston Univ Sch Med Boston MA 02118 USA

Ectonucleotidases influence purinergic receptor function by the hydrolysis of extracellular nucleotides, CD39 is an integral membrane protein that is a prototype member of the nucleoside 5'-triphosphate diphosphohydrolase family. The native CD39 protein has two intracytoplasmic and two transmembrane domains. There is a large extracellular domain that undergoes extensive glycosylation and can be post-translationally modified by limited proteolysis, We have identified a potential thioester linkage site for S-acylation within the N-terminal region of CD39 and demonstrate that this region undergoes palmitoylation in a constitutive manner. The covalent lipid modification of this region of the protein appears to be important both in plasma membrane association and in targeting CD39 to caveolae. These specialized plasmalemmal domains are enriched in G protein-coupled receptors and appear to integrate cellular activation events. We suggest that palmitoylation could modulate the function of CD39 in regulating cellular signal transduction pathways.

关键词：腺苷三磷酸酶类抗原 CD/代谢腺苷三磷酸双磷酸酶/代谢印迹法蛋白质 COS细胞细胞膜/代谢细胞培养的色谱法薄层细胞质/代谢内皮血管/酶学内皮血管/超微结构显微镜检查电子诱变棕榈酸/代谢信号传导转染动物人类

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Pathogenesis of African horse sickness:: Ultrastructural study of the capillaries in experimental infection

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JOURNAL OF COMPARATIVE PATHOLOGY 1999年第2期121卷 101-116页

作者： Gómez-Villamandos, JC Sánchez, C Carrasco, L Laviada, MD Bautista, MJ Martínez-Torrecuadrada, J Sánchez-Vizcaíno, JM Sierra, MA Univ Cordoba Fac Vet Dept Anat & Anat Patol Comparadas Cordoba 14005 Spain Inst Nacl Invest Agr Ctr Invest Sanidad Anim Madrid Spain Ingn Genet Aplicada SA Madrid 28037 Spain

African horse sickness (AHS) was induced in five horses by inoculation, to determine the ultrastructural changes in endothelial cells of capillaries in the myocardium, lung, spleen and liver. The animals developed cardiac and mixed forms of the disease. Alterations detected in the endothelial cells of the vessels of infected animals included: the presence of structures associated with viral infection, hypertrophy, degenerative changes, appearance of cytoplasmic projections, changes in permeability, alteration of intercellular junctions, loss of endothelium, subendothelial deposition of cell debris and fibrin, and vascular repair. In association with these changes, oedema, haemorrhages and microthromboses were detected, particularly in the myocardium and lung. This study showed that infection of, and changes to, the capillary endothelial cells of the organs under study was independent of the form in which the disease manifested itself but was dependent on the organ and blood vessel tl;pe Thus, different levels of viral tropism were observed for the endothelial cells of the vessels in different organs. Viral infection was commonest in the endothelial cells of myocardial vessels, followed by those in the lung, whereas in the spleen and liver, endothelial cell infection was rare and, in the case of the liver, limited to the interstitial capillaries. (C) 1999 W.B. Saunders Company Limited.

关键词：非洲马疾病/病理学毛细血管/病理学毛细血管/超微结构毛细血管通透性致细胞病变病毒细胞质/超微结构内皮血管/病理学内皮血管/超微结构马细胞间接合部/超微结构肝/血液供给肝/超微结构肺/血液供给肺/超微结构心肌/病理学心肌/超微结构脾/血液供给脾/超微结构动物女(雌)性

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RECENT STUDIES ON VASCULAR-ENDOTHELIUM

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ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1976年第AUG27期275卷 64-75页

作者： SIMIONESCU, N SIMIONESCU, M PALADE, GE YALE UNIV SCH MEDCELL BIOL SECTNEW HAVENCT 06510

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In vitro endothelialization of PTFE vascular grafts:: A comparison of various substrates, cell densities, and incubation times

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ANNALS OF VASCULAR SURGERY 1999年第2期13卷 141-150页

作者： Vinard, E Lesèche, G Andreassian, B Costagliola, D Hop Beaujon Serv Chirurg Vasc & Thorac F-92110 Clichy France

To establish the optimal conditions for achieving endothelial cell coverage of the luminal surfaces of small-caliber vascular grafts in vitro, the attachment of endothelial cells (ECs) cultured from human umbilical veins to polytetrafluoroethylene (PTFE) grafts was studied. Cell attachment and spreading were compared after PTFE grafts were (a) precoated with fibronectin (HFN), type I collagen, type IV collagen, plasma and fibrin with or without thrombin, singly or in combination;(b) seeded with cell densities varying from 0.5 x 10(5) to 6 x 10(5) cells/cm(2);and (c) incubated at 30, 60, or 90 min. Cell coverage and spreading were assessed by means of scanning electron microscopy. Quantification of graft surface coverage was performed with computer-assisted image analysis. To determine optimal conditions of endothelialization among the 189 treatment combinations, analysis of variance was used. We conclude that a virtually confluent cell monolayer can be established on small-caliber PTFE grafts when precoated with fibrin glue or plasma, seeded with cell densities greater than or equal to 4 x 10(5) cells/cm(2), and incubated for 60 min. These parameters are compatible with an operating room vascular procedure.

关键词：人工血管细胞计数细胞培养的内皮血管/细胞学内皮血管/超微结构显微镜检查电子扫描聚四氟乙烯时间因素脐静脉/细胞学人类

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Vascular anatomy of the rat ventral prostate

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ANATOMICAL RECORD 1999年第4期256卷 403-411页

作者： Shabsigh, A Tanji, N D'Agati, V Burchardt, T Burchardt, M Hyek, O Shabsigh, R Buttyan, R Columbia Univ Coll Phys & Surg Dept Urol New York NY 10032 USA Columbia Univ Coll Phys & Surg Dept Pathol New York NY 10032 USA

The rat ventral prostate gland is a model tissue to study the effects of androgenic steroids on prostate cells. Recent reports suggest that the prostatic vascular system is a primary target of androgen action in this tissue. In order to better understand how the vascular system of the ventral prostate supports the tissue in an androgenically normal adult male rat we utilized a variety of microscopic imaging techniques to more fully characterize its structural anatomy and its interaction with other prostatic cell types. Vascular corrosion casts were produced from the mature ventral prostate glands of rats. These casts were analyzed by scanning electron microscopy (SEM) to describe gross and fine details of the prostate vascular anatomy. Fixed thin sections of ventral prostates were immunostained with anti-Factor XIII and analyzed by Light microscopy for the presence of capillary elements within the prostatic glands. Other sections were directly analyzed by transmission electron microscopy (TEM) to describe the anatomical relationship between the capillaries and the prostatic ducts and their associated glands. The rat ventral prostate is supplied with blood by branches of the inferior vesical artery which enters the apex of the tissue from the base of the urinary bladder. Visualization of the prostatic vascular network under SEM shows that this major vessel is found on the posterior-medial surface of the tissue (closest to the bladder). This surface also has numerous serpentine vessels that appear to facilitate a stable blood supply to the prostate in accommodation of urinary bladder distension. Examination of the opposing surface of the casts allowed a crude description of the structure of the prostatic ductal system with the distal tips of the ducts (containing the prostate glands) oriented towards the anterior-lateral surface of the tissue. On this surface, one can discern a series of adjacent basket-shaped vascular structures of distributing arterioles that supply

关键词：血管/解剖学和组织学血管/超微结构腐蚀铸型/方法显微镜检查电子扫描前列腺/血液供给大鼠 Sprague-Dawley 动物男(雄)性大鼠

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Altered structure and mechanics of skeletal muscle arteries with high-salt diet and reduced renal mass hypertension

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MICROVASCULAR RESEARCH 2000年第2期59卷 323-328页

作者： Frisbee, JC Weber, DS Liu, Y DeBruin, JA Lombard, JH Med Coll Wisconsin Dept Physiol Milwaukee WI 53226 USA

It is well established that hypertension alters the mechanics and composition of arterial walls in many vascular beds {Cox, 1989). Although it has been previously accepted that hypertension reduces arterial distensibility, previous studies indicate that these alterations can be variable and depend upon the specific vessel, its size, and the etiology of the hypertension (Baumbach and Hajdu, 1993; Brayden et al, 1983; Win-quist and Bohr, 1983). Ongoing studies in our laboratory have demonstrated that, in addition to the effectsof reduced renal mass hypertension (RRMHT), high-salt diet alone (independent of blood pressure) may have considerable effects on the structure of skeletal muscle arterioles that could significantly impact the mechanics of the vessel wall (Frisbee and Lombard, 1998). In addition, Hansen-Smith et al. (1991) reported that structural alterations to individual vascular smooth muscle cells develop in skeletal muscle arterioles of rats with RRMHT and in normotensive rats on a high-salt diet. However, changes in the structure and passive wall mechanics of skeletal muscle resistance arteries of rats with RRMHT and in normotensive rats on a high-salt diet have not been *** present study tested the hypothesis that RRMHT and/or a high salt diet lead to significant alterations in the structure and passive mechanics (i.e., an increased wall stiffness and reduced distensibility) of the small muscular arteries that lie immediately upstream from the skeletal muscle microcirculation and have a major role in controlling the resistance to blood flow in that vascular bed.

关键词：胶原/分析弹性股动脉/病理学高血压/化学诱导高血压/病因学高血压/病理学肌骨骼/血液供给肌骨骼/病理学肌平滑血管/化学肌平滑血管/超微结构氯化钠膳食/毒性血管阻力动物大鼠

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