Purpose: This study sought to evaluate the effect of repeated implant drilling on the immediate bone-cell viability, and to evaluate drill wear by scanning electron microscopy. Materials and Methods: The tibiae of 10 ...
详细信息
Purpose: This study sought to evaluate the effect of repeated implant drilling on the immediate bone-cell viability, and to evaluate drill wear by scanning electron microscopy. Materials and Methods: The tibiae of 10 rabbits were used, divided into 5 groups (G): G1 corresponded to new drills, and G2, G3, G4, and G5 corresponded to drills used 10, 20, 30, and 40 times, respectively. The animals received 10 sequential osteotomies in each tibia. The animals were euthanized immediately after the osteotomies by perfusion with 4% formaldehyde. Samples then underwent immunohistochemistry processing for ordinal qualitative analysis of osteoprotegerin (OPG), the RANK ligant (RANKL;a tumor-related necrosis factor receptor family), and osteocalcin protein immunolabels, as detected by the immunoperoxidase method and revealed with 3,3-diaminobenzidine. Drill wear and plastic deformation were analyzed by scanning electron microscopy (SEM). Results: The proteins were expressed in osteocytes of the superior bone cortical during the 40 drillings. However, in G4 and G5, a discrete increase in the expression of RANKL was observed, when compared with OPG;this increase was statistically significant in G5 (P = .016). The SEM analysis revealed major plastic deformation and drill wear in G4 and G5. Conclusion: Based on the present methodology, it may be concluded that cell viability is preserved if a less traumatic surgical protocol is used. However, the repeated use of drills alters the protein balance as of the thirtieth perforation. (C) 2008 American Association of Oral and Maxillofacial Surgeons.
Background: Proper disposal of sharps in the clinical setting is a key factor in infection control. Previous research studies suggest that reprocessed, reusable medical devices and infectious waste containers are pote...
详细信息
Background: Proper disposal of sharps in the clinical setting is a key factor in infection control. Previous research studies suggest that reprocessed, reusable medical devices and infectious waste containers are potential sources of microorganisms capable of causing infection in immunocompromised patients. This pilot study was a single-center, prospective, hospital-based, microbiologic evaluation of reusable sharps disposal containers returned to the hospital from a reprocessing company. Methods: A New England area, 130-bed community hospital performed the evaluation. Following delivery to the hospital's shipping/receiving area, 30 newly processed, reusable sharps disposal containers were swabbed for the presence of bacteria and viruses. Results: Twenty-seven containers (90%) tested positive for bacteria, and 10% of the recovered isolates were gram-negative rods. Nine out of 30 (30%) cultures were positive for viruses: HIV (10%), hepatitis A (6.7%), hepatitis B (6.7%), and hepatitis C (13.3%), and several containers tested positive for multiple viruses and bacteria. Conclusion: Reusable sharps containers were returned to this medical facility with bacterial and viral contamination. Further testing is warranted to determine the scope of the problem and potential clinical implications.
暂无评论