检索结果-内蒙古大学图书馆

在昆明地区秋冬季腹泻病儿童粪便中发现轮状病毒的初步报告

引用

云南医药 1980年第2期 37-39页

来源：评论

学校读者我要写书评

暂无评论

婴幼儿腹泻的病原学研究：轮状病毒(rotarirus)提取与鉴定

引用

黑龙江医药 1980年第4期 32-34页

来源：评论

学校读者我要写书评

暂无评论

轮状病毒肠炎患儿的两种检测方法比较

引用

中国误诊学杂志 2011年第24期11卷 5865-5866页

作者：徐亮李萍陈建芳卞新南南京医科大学附属苏州医院苏州市立医院江苏苏州215001

目的研究粪便中轮状病毒病原体的两种快速检测方法的比较。方法荧光定量RT-PCR方法检测轮状病毒核酸,与WHO推荐的轮状病毒检测方法酶联免疫测定平行检测,对比评价。结果荧光定量RT-PCR体系具有高度灵敏度和特异性,与酶联免疫测定方法的... 详细信息

目的研究粪便中轮状病毒病原体的两种快速检测方法的比较。方法荧光定量RT-PCR方法检测轮状病毒核酸,与WHO推荐的轮状病毒检测方法酶联免疫测定平行检测,对比评价。结果荧光定量RT-PCR体系具有高度灵敏度和特异性,与酶联免疫测定方法的检出率差异有统计学意义(2χ=42,P<0.01)。结论轮状病毒荧光定量RT-PCR体系优于酶联免疫测定方法,可用于临床检测。对及时明确病因,防止轮状病毒暴发、流行,做好公共卫生工作具有重要意义。

关键词：肠炎/微生物学轮状病毒属/分离和提纯逆转录聚合酶链反应酶联免疫吸附测定

来源：评论

学校读者我要写书评

暂无评论

INVITRO TRANSCRIPTION AND TRANSLATION OF GENOMIC RNA FROM A PORCINE GROUP-C ROTAVIRUS - BRIEF REPORT

引用

ARCHIVES OF VIROLOGY 1992年第1-2期124卷 181-185页

作者： JIANG, BM SAIF, LJ OHIO STATE UNIV OHIO AGR RES & DEV CTRFOOD ANIM HLTH RES PROGRAM1680 MADISON AVEWOOSTEROH 44691

Eleven segments of ssRNA were synthesized from dsRNAs of a porcine group (Gp) C rotavirus (Cowden strain) using an in vitro transcription system. In vitro translation of unfractionated ssRNAs revealed at least nine viral proteins, ranging from 22 kDa to 93 kDa. The 37 kDa and 25 kDa proteins were glycosylated as demonstrated by the endoglycosidase H assay. In vitro translated products analyzed by SDS-polyacrylamide gel electrophoresis and partial protease peptide mapping were comparable to those synthesized in vivo (MA 104 cells).

关键词：无细胞系统粪便/微生物学肽谱蛋白质生物合成轮状病毒属/分离和提纯轮状病毒属/代谢猪/微生物学转录遗传病毒蛋白质类/生物合成病毒蛋白质类/分离和提纯动物

来源：评论

学校读者我要写书评

暂无评论

First detection of bovine group B rotavirus in Japan and sequence of its VP7 gene

引用

ARCHIVES OF VIROLOGY 1999年第4期144卷 805-815页

作者： Tsunemitsu, H Morita, D Takaku, H Nishimori, T Imai, K Saif, LJ Natl Inst Anim Hlth Shichinohe Res Unit Shichinohe Aomori 0392586 Japan Natl Inst Anim Hlth Hokkaido Res Stn Sapporo Hokkaido Japan Nemuro Livestock Hyg Ctr Betsukai Hokkaido Japan Ohio State Univ Ohio Agr Res & Dev Ctr Food Anim Hlth Res Program Wooster OH 44691 USA

An epizootic outbreak of diarrhea occulted in adult cows on a dairy farm in Hokkaido, Japan. One colostrum-fed calf inoculated with pooled feces of the 5 affected cows, developed mild diarrhea, and shed rotavirus-like particles which reacted with antiserum to group B rotavirus in immune electron microscopy. Cell culture immunofluorescence tests, RNA-polyacrylamide gel electrophoresis and RT-PCR confirmed that this virus was bovine group B rotavirus, which was designated the Nemuro strain. Additional 2 colostrum-deprived calves inoculated with feces of the first calf also developed diarrhea and shed virus, suggesting that this group B rotavirus might be the etiological agent of the outbreak of adult cow diarrhea. The identities of the nucleotide (nt) and deduced amino acid (aa) sequences of the Nemuro VP7 gene were high (93-95% in nt and 96-97% in aa) and low (61-63% in nt and 49-61% in aa) compared to those of the published corresponding genes from 3 bovine and 2 other mammalian (human and rat) strains of group B rotaviruses, respectively. To our knowledge, this is the first report on the presence of bovine group B rotavirus in Japan.

关键词：氨基酸序列抗原病毒/遗传学病毒壳体/遗传学衣壳蛋白质类牛疾病/流行病学牛疾病/传播牛疾病/病毒学细胞系初乳腹泻/流行病学腹泻/兽医学腹泻/病毒学疾病暴发流行/兽医学粪便/病毒学日本/流行病学分子序列数据轮状病毒属/分类轮状病毒属/遗传学轮状病毒属/分离和提纯轮状病毒感染/流行病学轮状病毒感染/传播轮状病毒感染/兽医学序列比对序列同源性氨基酸病毒脱落动物牛女(雌)性人类大鼠

来源：评论

学校读者我要写书评

暂无评论

Demonstration of group C rotaviruses in fecal samples of diarrheic dogs in Germany

引用

ARCHIVES OF VIROLOGY 1999年第12期144卷 2467-2473页

作者： Otto, P Schulze, P Herbst, W Fed Inst Hlth Protect Consumers & Vet Med Div 4 Jena Germany Fed Inst Hlth Protect Consumers & Vet Med Div 5 Berlin Germany Univ Giessen Inst Hyg & Infect Dis Anim Giessen Germany

Three out of 9 fecal samples from diarrheic dogs, which were positive for rotavirus by electron microscopy, revealed RNA-migration patterns identical to those of porcine group C rotavirus when studied by polyacrylamide gel electrophoresis (PAGE). The results were further confirmed by solid phase immune electron microscopy (SPIEM). This is the first report of the occurrence of group C rotaviruses in dogs.

关键词：腹泻/兽医学腹泻/病毒学狗疾病/病毒学电泳聚丙烯酰氨凝胶粪便/病毒学德国显微镜检查电子显微镜检查免疫电子/方法 RNA 病毒/分析轮状病毒属/分离和提纯轮状病毒感染/兽医学轮状病毒感染/病毒学动物狗

来源：评论

学校读者我要写书评

暂无评论

EXCLUSIVE ASYMPTOMATIC NEONATAL INFECTIONS BY HUMAN ROTAVIRUS STRAINS HAVING SUBGROUP-I SPECIFICITY AND LONG RNA ELECTROPHEROTYPE

引用

ARCHIVES OF VIROLOGY 1992年第1-4期126卷 239-251页

作者： SUKUMARAN, M GOWDA, K MAIYA, PP SRINIVAS, TP KUMAR, MS AIJAZ, S REDDY, RR PADILLA, L GREENBERG, HB RAO, CD INDIAN INST SCI DEPT MICROBIOL & CELL BIOL BANGALORE 560012 KARNATAKA INDIA MSR MED COLL HOSP BANGALORE INDIA ST MARTHAS HOSP BANGALORE INDIA STANFORD UNIV MED CTR SCH MED DIV GASTROENTEROL STANFORD CA 94305 USA INDIAN INST SCI CTR GENET ENGN BANGALORE 560012 KARNATAKA INDIA

A large number of stool specimes, of healthy new born infants, collected from various hospitals and clinics in Bangalore City, India, have been examined for the presence of asymptomatic rotaviral excretion. Out of 370 samples analysed during a three year period from 1988 to 1991, 133 specimens (36%) were positive for rotavirus RNA. All these asymptomatic neonatal strains, without exception, showed "long" RNA pattern, but subgroup I specificity. Serotype analysis by ELISA or by hybridization with serotype-specific probes indicated that these strains probably represent a new serotype in newborn children. We f-ind an exclusive association of human rotaviruses having "long" RNA pattern and subgroup I specificity with asymptomatic neonatal infections in contrast to the earlier observations of association of such unusual strains with acute gastroenteritis in young children.

关键词：抗原病毒病毒壳体/遗传学病毒壳体/免疫学衣壳蛋白质类 DNA探针/遗传学酶联免疫吸附测定粪便/微生物学印度/流行病学 RNA 病毒/遗传学轮状病毒属/分类轮状病毒属/遗传学轮状病毒属/免疫学轮状病毒属/分离和提纯轮状病毒感染/流行病学轮状病毒感染/微生物学血清分型人类婴儿新生

来源：评论

学校读者我要写书评

暂无评论

A FAMILY OUTBREAK OF GASTROENTERITIS CAUSED BY GROUP-C ROTAVIRUS

引用

ARCHIVES OF VIROLOGY 1992年第3-4期124卷 269-278页

作者： MAUNULA, L SVENSSON, L VONBONSDORFF, CH NATL BACTERIOL LAB S-10521 STOCKHOLMSWEDEN

A family outbreak of gastroenteritis caused by group C rotavirus described. All five members of the family, with children between 8 and 15 years of age, fell ill with diarrhea. The diagnosis was initially based on the detection of rotavirus RNA showing a typical group C profile in gel electrophoresis in stool samples, and it was serologically verified from patient sera using a cell culture adapted porcine group C rotavirus as a source of standard antigen. All collected serum samples from the family contained IgM and/or IgG class antibodies to group C rotavirus measurable by immunofluorescence antibody test (IFAT). Group C rotavirus specific IgM class antibodies were present in the early serum samples in 3/4 patients. A roller tube neutralization test (NT) was established to demonstrate neutralizing antibodies to porcine group C rotavirus in human sera. These methods can be used to detect serologically group C rotavirus infections.

关键词：抗体病毒/血液腹泻/微生物学疾病暴发流行粪便/微生物学芬兰/流行病学荧光抗体技术胃肠炎/微生物学中和试验轮状病毒属/分离和提纯轮状病毒属/超微结构轮状病毒感染/微生物学青少年儿童女(雌)性人类男(雄)性中年人

来源：评论

学校读者我要写书评

暂无评论

Characterization of human symptomatic rotavirus isolates MP409 and MP480 having 'long' RNA electropherotype and subgroup I specificity, highly related to the P6,G8 type bovine rotavirus A5, from Mysore, India

引用

ARCHIVES OF VIROLOGY 2000年第7期145卷 1339-1357页

作者： Jagannath, MR Vethanayagam, RR Reddy, BSY Raman, S Rao, CD Indian Inst Sci Dept Microbiol & Cell Biol Bangalore 560012 Karnataka India

In an epidemiological study of symptomatic human rotaviruses in Mysore, India during 1993 and 1994, isolates MP409 and MP480 were isolated from two children suffering from severe, acute dehydrating diarrhea. Both isolates exhibited 'long' RNA pattern and subgroup I specificity suggesting the likelihood of their animal origin. Both isolates did not react with monoclonal antibodies (MAbs) specific for serotypes G1 to G6 as well as CIO. To determine the genetic origin of these isolates, complete nucleotide sequences of genes encoding the outer capsid proteins VP4 and VP7, nonstructural proteins NSP1 and NSP3 and viral enterotoxin protein NSP4 from MP409 and partial sequences of genes from MP480 were determined. Comparison of the 5' and 3' terminal sequences of 250 nucleotides revealed complete identity of the gene sequences in both strains suggesting that MP409 and MP480 are two different isolates of a single strain. Comparison of the nucleotide and deduced amino acid sequences of VP4, VP7, NSP1 and NSP3 of MP409 with published sequences of strains belonging to different serotypes revealed that both outer capsid proteins VP4 and VP7 and NSP1 are highly related to the respective proteins from the P6[1], G8 type bovine rotavirus A5 isolated from a calf with diarrhoea in Thailand and that the NSP3 is highly homologous to that of bovine rotaviruses. The NSP 1 protein showed greatest sequence identity with NSP4s belonging to the KUN genetic group to which NSP4s from human G2 type strains and bovine rotaviruses belong. MP409 and MP480 likely signify interspecies transmission of P6[1], G8 type strains from cattle to humans and represent the first P6[1] type rotaviruses isolated in humans. These and our previous studies on the asymptomatic neonatal strain I321 are of evolutionary and epidemiological significance in the context of close association of majority of the Indian population with cattle.

关键词：氨基酸序列基因组病毒印度分子序列数据 RNA 病毒/遗传学轮状病毒属/分类轮状病毒属/遗传学轮状病毒属/分离和提纯序列比对序列分析病毒蛋白质类/遗传学动物牛人类

来源：评论

学校读者我要写书评

暂无评论

Ultrasonographic assessment of Salmonella enterocolitis in children

引用

PEDIATRIC RADIOLOGY 1999年第6期29卷 469-471页

作者： Ueda, D Sato, T Yoshida, M Sapporo Higashi Tokushukai Hosp Dept Paediat Higashi Ku Sapporo Hokkaido 065 Japan

Background. Salmonella enterocolitis (SE) is one of the important causes of acute infectious diarrhoea. Imaging studies are rarely performed on these patients. Consequently, ultrasound (US) features of SE are controversial. Objective. To identify the clinical significance of US in the evaluation of SE. Material and methods. Abdominal US was performed in 15 patients with SE and 9 patients with Rotavirus enterocolitis (RE). Results. Ascites was present in 60 % and mural thickening of the colon in 40 % of patients with SE on abdominal US, whereas we could not identify these features in patients with RE. In patients with SE, colonic wall thickening;and ascites, the levels of C-reactive protein (CRP) were significantly higher as compared to patients with SE and no colonic wall thickening or ascites. Also, the stool occult blood test was positive more often in patients with colonic wall thickening and ascites on US than in patients without these findings. The colonic wall thickness significantly correlated with CRP and stool occult blood level. Conclusions. US is able to identify pathological changes in bowel and intra-abdominal spaces. The US findings of ascites and colonic wall thickening may be useful for determining the severity of SE.

关键词：腹水/病因学腹水/超声检查诊断鉴别小肠结肠炎/并发症小肠结肠炎/超声检查粪便/微生物学粪便/病毒学随访研究潜血回顾性研究轮状病毒属/分离和提纯轮状病毒感染/并发症轮状病毒感染/超声检查沙门菌属/分离和提纯沙门菌感染/并发症沙门菌感染/超声检查疾病严重程度指数儿童学龄前女(雌)性人类婴儿男(雄)性

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：