检索结果-内蒙古大学图书馆

中山大学学报（医学科学版） 1996年第S1期16卷 66-68页

作者：徐瑞华管忠震何友兼姜文奇喻丽华廖海中山医科大学肿瘤医院内科

ｒｈＧ－ＣＳＦ扩增外周血造血祖细胞徐瑞华，管忠震，何友兼，姜文奇，喻丽华，廖海（中山医科大学肿瘤医院内科；广州，５１００６０）主题词粒细胞集落刺激因子／药物疗法，造血干细胞／药物作用中图号Ｒ９７３．４；３３１．１４；... 详细信息

ｒｈＧ－ＣＳＦ扩增外周血造血祖细胞徐瑞华，管忠震，何友兼，姜文奇，喻丽华，廖海（中山医科大学肿瘤医院内科；广州，５１００６０）主题词粒细胞集落刺激因子／药物疗法，造血干细胞／药物作用中图号Ｒ９７３．４；３３１．１４；７３０．５９自体外周血造血干细胞移...

关键词：粒细胞集落刺激因子/药物疗法造血干细胞/药物作用

来源：评论

学校读者我要写书评

暂无评论

Utilizing granulocyte colony-stimulating factor to enhance vascular graft endothelialization from circulating blood cells

引用

ANNALS OF VASCULAR SURGERY 2002年第3期16卷 314-320页

作者： Shi, Q Bhattacharya, V Hong-De Wu, M Sauvage, LR Providence Seattle Med Ctr Hope Heart Inst Seattle WA 98122 USA Univ Washington Sch Med Dept Surg Seattle WA 98195 USA

Cells in the blood circulating through a vascular graft can contribute to endothelialization of its flow surface. We hypothesized that granulocyte colony-stimulating factor (G-CSF) could enhance this process by increasing circulating bone marrow progenitor cells. Ten dogs received composite grafts that were shielded from any source of endothelialization other than the circulating blood. On the seventh postoperative day and for 7 days thereafter, five dogs were injected subcutaneously with 10 mug/kg/day of human G-CSF. The additional five dogs, used as controls, received no G-CSF. Grafts were retrieved at 4 weeks. All dogs recovered promptly postoperatively. White cell counts in G-CSF dogs increased by an average of 9.5-fold at the end of treatment, and had returned to normal before retrieval. All grafts remained patent. G-CSF grafts had significantly higher endothelialization than the controls (82.2 +/- 9.2% vs. 23.7 +/- 4.4%, p = 0.0004), with extensive flow surface neointima, covered with a single layer of endothelium verified by FVIII/vWF and CD34 staining. Control grafts had virtually no neointima and were covered with a thin layer of fibrin coagulum. Significantly more endothelial-lined microvessels were also found in the G-CSF grafts than in the controls. Dogs treated with G-CSF have increased endothelialization of synthetic vascular grafts due to increased circulating bone marrow progenitor cells.

关键词：人工血管血管假体植入内皮血管/药物作用粒细胞集落刺激因子/药理学造血干细胞/药物作用白细胞计数血管未闭动物狗女(雌)性男(雄)性

来源：评论

学校读者我要写书评

暂无评论

Functional characterization of B lymphocytes generated in vitro from embryonic stem cells

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1999年第17期96卷 9797-9802页

作者： Cho, SK Webber, TD Carlyle, JR Nakano, T Lewis, SM Zúñiga-Pflücker, JC Univ Toronto Dept Immunol Toronto ON M5S 1A8 Canada Osaka Univ Microbial Dis Res Inst Dept Mol Cell Biol Suita Osaka 565 Japan Hosp Sick Children Program Genet & Genom Biol Res Inst Toronto ON M5G 1X8 Canada

To study molecular events involved in B lymphocyte development and V(D)J rearrangement, we have established an efficient system for the differentiation of embryonic stem (ES) cells into mature Ig-secreting B lymphocytes. Here, we show that B lineage cells generated in vitro from ES cells are functionally analogous to normal fetal liver-derived or bone marrow-derived B lineage cells at three important developmental stages: first, they respond to Flt-3 ligand during an early lymphopoietic progenitor stage;second, they become targets for Abelson murine leukemia virus (A-MuLV) infection at a pre-B cell stage;third, they secrete Ig upon stimulation with lipopolysaccharide at a mature mitogen-responsive stage. Moreover, the ES cell-derived A-MuLV-transformed pre-B (EAB) cells are phenotypically and functionally indistinguishable from standard A-MuLV-transformed pre-B cells derived from infection of mouse fetal liver or bone marrow. Notably, EAB cells possess functional V(D)J recombinase activity. In particular, the generation of A-MuLV transformants from ES cells will provide an advantageous system to investigate genetic modifications that will help to elucidate molecular mechanisms in V(D)J recombination and in A-MuLV-mediated transformation.

关键词： Abelson鼠白血病病毒 B淋巴细胞/生理学细胞分化/药物作用共同培养技术基因重排 B淋巴细胞血细胞生成造血干细胞/药物作用造血干细胞/生理学配体膜蛋白质类/药理学聚合酶链反应动物小鼠

来源：评论

学校读者我要写书评

暂无评论

Interleukin-3 supports expansion of long-term multilineage repopulating activity after multiple stem cell divisions in vitro

引用

BLOOD 2000年第5期96卷 1748-1755页

作者： Bryder, D Jacobsen, SEW Univ Lund Hosp Inst Lab Med Stem Cell Lab S-22185 Lund Sweden

Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. This has triggered the search for the optimal combination of cytokines for HSC expansion. Through such studies, c-kit ligand (KL), flt3 ligand (FL), thrombopoietin, and IL-ll have emerged as likely positive regulators of HSC self-renewal. In contrast, numerous studies have implicated a unique and potent negative regulatory role of IL-3, suggesting perhaps distinct regulation of Introduction HSC fate by different cytokines, However, the interpretations of these findings are complicated by the fact that different cytokines might target distinct subpopulations within the HSC compartment and by the lack of evidence for HSC undergoing self-renewal. Here, in the presence of KL+FL+megakaryocyte growth and development factor (MGDF), which recruits virtually all Lin(-)Sca-1(+)kit(+) bone marrow cells into proliferation and promotes their self-renewal under serum-free conditions, IL-3 and IL-ll revealed an indistinguishable ability to further enhance proliferation. Surprisingly, and similar to IL-ll, IL-3 supported KL+FL+MGDF-induced expansion of multilineage, long-term reconstituting activity in primary and secondary recipients. Furthermore, high-resolution cell division tracking demonstrated that all HSC underwent a minimum of 5 cell divisions, suggesting that long-term repopulating HSC are not compromised by IL-3 stimulation after multiple cell divisions. In striking contrast, the ex vivo expansion of murine HSC in fetal calf serum-containing medium resulted in extensive loss of reconstituting activity, an effect further facilitated by the presence of IL-3. (C) 2000 by The American Society of Hematology.

关键词：抗原 CD34/血液抗原 Ly/血液细胞分裂/药物作用细胞谱系细胞培养的流式细胞术造血干细胞移植造血干细胞/化学造血干细胞/细胞学造血干细胞/药物作用白细胞介素11/药理学白细胞介素3/药理学膜蛋白质类/血液膜蛋白质类/药理学小鼠同源小鼠近交C57BL 小鼠近交系原癌基因蛋白质c-kit/血液重组蛋白质类/药理学干细胞因子/药理学血小板生成素/药理学时间因素动物人类小鼠

来源：评论

学校读者我要写书评

暂无评论

Nf1 and Gmcsf interact in myeloid leukemogenesis

引用

MOLECULAR CELL 2000年第1期5卷 189-195页

作者： Birnbaum, RA O'Marcaigh, A Wardak, Z Zhang, YY Dranoff, G Jacks, T Clapp, DW Shannon, KM Univ Calif San Francisco Dept Pediat San Francisco CA 94143 USA Indiana Univ Sch Med Dept Pediat Indianapolis IN 46202 USA Indiana Univ Sch Med Herman B Wells Ctr Indianapolis IN 46202 USA Harvard Univ Sch Med Dana Farber Canc Inst Dept Adult Oncol Boston MA 02115 USA MIT Howard Hughes Med Inst Dept Biol Cambridge MA 02139 USA

The NF1 tumor suppressor gene encodes neurofibromin, a GTPase-activating protein (GAP) for p21(ras) (Ras). Children with NF1 are predisposed to juvenile myelomonocytic leukemia (JMML). Some heterozygous Nf1 mutant mice develop a similar myeloproliferative disorder (MPD), and adoptive transfer of Nf1-deficient fetal liver cells consistently induces this MPD. Human JMML and murine Nf1-deficient cells are hypersensitive to granulocyte-macrophage colony-stimulating factor (GM-CSF) in methylcellulose cultures. We generated hematopoietic cells deficient in both Nf1 and Gmcsf to test whether GM-CSF is required to drive excessive proliferation of Nf1(-/-) cells in vivo. Here we show that GM-CSF plays a central role in establishing and maintaining the MPD and that recipients engrafted with Nf1(-/-) Gmcsf(-/-) hematopoietic cells are hypersensitive to exogenous GM-CSF.

关键词：过继转移细胞分裂交叉遗传基因神经纤维瘤病1型疾病遗传易感性粒细胞巨噬细胞集落刺激因子/缺乏粒细胞巨噬细胞集落刺激因子/遗传学粒细胞巨噬细胞集落刺激因子/药理学造血干细胞移植造血干细胞/细胞学造血干细胞/药物作用杂合子白血病粒-单核细胞慢性/遗传学白血病粒-单核细胞慢性/病理学肝/胚胎学小鼠近交BALBC 小鼠近交系小鼠基因敲除神经纤维素1 蛋白质类/遗传学动物儿童女(雌)性人类男(雄)性小鼠

来源：评论

学校读者我要写书评

暂无评论

Tissue- and epitope-specific mechanisms account for the diverse effects of anti-CD44 antibodies on the maintenance of primitive hematopoietic progenitors in vitro

引用

BLOOD CELLS MOLECULES AND DISEASES 2000年第4期26卷 291-302页

作者： Müller-Sieburg, CE Deryugina, E Khaldoyanidi, S O'Rourke, A Sidney Kimmel Canc Ctr San Diego CA 92121 USA Burnham Inst La Jolla CA 92037 USA Scripps Res Inst La Jolla CA USA

The identification of rare stromal cells that support high levels of stem cells has opened avenues to identify molecules that contribute to the maintenance of these cells. We show that the maintenance of long-term culture initiating cells (LTC-IC) in stromal cell-supported cultures can be modulated via mAbs specific for CD44. mAb IM7.8.1 suppressed while mAb RAMBM44 enhanced LTC-IC levels in culture. Genetic polymorphisms in CD44 were used to show that the stromal cell compartment is targeted by mAb RAMBM44 and the hematopoietic compartment by mAb IM7.8. Neither of the CD44-specific mAbs inhibited adhesion of LTC-IC to the stroma, suggesting alternative mechanisms of action. In support of this interpretation, we show that mAb RAMBM44 directly induces signal transduction in the stromal cell line S17 but not in hematopoietic cells. Conversely, mAb IM7.8 elicited the appearance of phosphorylated bands in hematopoietic cells, but not in stromal cells. Collectively, the data indicate that the opposing effects of CD44-mediated regulation can be explained by different cellular programs that are elicited in distinct cell compartments. The binding of the enhancing mAb RAMBM44 to CD44 is specifically inhibited by collagen TV, while binding of the suppressive mAb IM7.8.1 is inhibited by a substance contained in the supernatant of the stromal cell line AC3.U. Thus, the CD44 epitopes defined by the mAbs bind distinct ligands and the ligands provide a potential physiological counterpart for the regulatory actions Of the mAbs. (C) 2000 Academic Press.

关键词：抗体单克隆/药理学抗原 CD44/免疫学细胞黏附/药物作用细胞间通讯/药物作用细胞培养的共同培养技术表位/免疫学造血干细胞/细胞学造血干细胞/药物作用配体小鼠近交C57BL 小鼠近交DBA 小鼠基因敲除信号传导间质细胞/细胞学动物女(雌)性男(雄)性小鼠

来源：评论

学校读者我要写书评

暂无评论

Myeloblastin is a granulocyte colony-stimulating factor-responsive gene conferring factor-independent growth to hematopoietic cells

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2000年第4期97卷 1601-1606页

作者： Lutz, PG Moog-Lutz, C Coumau-Gatbois, E Kobari, L Di Gioia, Y Cayre, YE Hop St Antoine INSERM U417 F-75012 Paris France Thomas Jefferson Univ Kimmel Canc Inst Dept Microbiol Immunol Philadelphia PA 19107 USA

Hematopoiesis depends on a pool of quiescent hematopoietic stem/progenitor cells. When exposed to specific cytokines, a portion of these cells enters the cell cycle to generate an amplified progeny. Myeloblastin (MBN) initially was described as involved in proliferation of human leukemia cells. The granulocyte colony-stimulating factor (G-CSF), which stimulates the proliferation of granulocytic precursors, up-regulates MBN expression. Here we show that constitutive overexpression of MBN confers factor-independent growth to murine bone marrow-derived Ba/F3/G-CSFR cells. Our results point to MBN as a C-CSF responsive gene critical to factor-independent growth and indicate that expression of the G-CSF receptor is a prerequisite to this process. A 91-bp MBN promoter region containing PU.1, C/EBP, and c-Myb binding sites is responsive to G-CSF treatment. Although PU.1. C/EBP, and c-Myb transcription factors all were critical for expression of MEN, its up-regulation by G-CSF was associated mainly with PU.1. These findings suggest that MBN is an important target of PU.1 and a key protease for factor-independent growth of hematopoietic cells.

关键词：抗原 CD34/免疫学 CCAAT增强子结合蛋白类 DNA结合蛋白质类/代谢基因表达调控粒细胞集落刺激因子/药理学造血干细胞/药物作用成髓细胞素核蛋白质类/代谢启动区遗传原癌基因蛋白质类/代谢原癌基因蛋白质c-myb/代谢受体粒细胞集落刺激因子/代谢丝氨酸内肽酶类/遗传学丝氨酸内肽酶类/代谢反式激活因子类/代谢转录因子/代谢转染肿瘤细胞培养的动物人类小鼠

来源：评论

学校读者我要写书评

暂无评论

Interleukin-7 stimulates osteoclast formation by up-regulating the T-cell production of soluble osteoclastogenic cytokines

引用

BLOOD 2000年第5期96卷 1873-1878页

作者： Weitzmann, MN Cenci, S Rifas, L Brown, C Pacifici, R Barnes Jewish Hosp Div Bone & Mineral Dis St Louis MO 63110 USA Washington Univ Sch Med Div Bone & Mineral Dis St Louis MO USA Univ Calgary Div Hematol Calgary AB Canada

In unstimulated conditions osteoclast renewal occurs as a result of the stromal cell production of the key osteoclastogenic factors, receptor activator of NFkB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), Inflammation is known to cause increased osteoclastogenesis;however, the mechanisms responsible for this phenomenon are poorly understood. We now show that interleukin-1 (II-1) and tumor necrosis factor alpha (TNF alpha), cytokines typically produced in inflammatory conditions, increase the stromal cell production of IL-7, This factor, in turn, up-regulates production of osteoclastogenic cytokines by T cells leading to stimulation of osteoclast (OC) formation. Although T cells were found to produce soluble forms of both RANKL and M-CSF, saturating concentrations of osteoprotegerin failed to inhibit approximately 40% of the OC formation, suggesting that IL-7 acts via both RANKL-dependent and RANKL-independent pathways, Despite the identification of T-cell-secreted M-CSF, this cytokine was not essential for either RANKL-dependent or -independent OC formation, suggesting that T cells secrete other cytokines capable of substituting for M-CSF action, On the basis of our data, we propose a novel mechanism for inflammatory bone loss in which induction of IL-7 from stromal cells by IL-l and TNF alpha leads to the production of soluble osteoclastogenic cytokines by T cells. Thus, the mechanism by which IL-7 causes bone resorption involves the activation of T cells and the T-cell-dependent augmentation of osteoclastogenesis, (C) 2000 by The American Society of Hematology.

关键词：酸性磷酸酶/药物作用酸性磷酸酶/代谢载体蛋白质类/药物作用载体蛋白质类/遗传学载体蛋白质类/代谢细胞培养的细胞因子类/药物作用细胞因子类/代谢剂量效应关系药物造血干细胞/细胞学造血干细胞/药物作用白细胞介素1/药理学白细胞介素7/代谢白细胞介素7/药理学同工酶类/药物作用同工酶类/代谢膜糖蛋白类/药物作用膜糖蛋白类/遗传学膜糖蛋白类/代谢成骨细胞/细胞学成骨细胞/药物作用成骨细胞/代谢 RANK配体 RNA 信使/药物作用 RNA 信使/遗传学 RNA 信使/代谢核因子κB受体活化因子重组蛋白质类/代谢重组蛋白质类/药理学间质细胞/细胞学间质细胞/药物作用间质细胞/代谢 T淋巴细胞/细胞学 T淋巴细胞/药物作用 T淋巴细胞/代谢肿瘤坏死因子α/药理学增量调节人类

来源：评论

学校读者我要写书评

暂无评论

Distinct signals control the hematopoiesis of lymphoid-related dendritic cells

引用

BLOOD 2000年第1期95卷 128-137页

作者： Galy, A Christopherson, I Ferlazzo, G Liu, G Spits, H Georgopoulos, K Wayne State Univ Barbara Ann Karmanos Canc Inst Detroit MI 48201 USA Netherlands Canc Inst Amsterdam Netherlands Harvard Univ Sch Med Massachusetts Gen Hosp Cutaneous Biol Res Ctr Charlestown MA USA

The molecular and cellular requirements for the development of different populations of human dendritic cells (DC) were studied. Conditions were defined that support DC production from lymphoid progenitors but that fail to induce DC formation from peripheral monocytes. The production of these lymphoid-related DC was severely blocked when hematopoietic progenitors overexpressed Ik7, a mutant dominant-negative Ikaros protein. In contrast, Ik7 did not block the formation of DC in conditions supporting the development of monocyte-derived DC. Furthermore, Ik7 did not block the formation of monocyte/macrophages and enhanced granulopoiesis. One of the molecular mechanisms mediated by Ik7 appears to be down-regulation of the flt3-receptor mRNA, Thus, distinct signals control the formation of DC demonstrating that some aspects of DC diversity are determined in part by distinct molecular cues at the hematopoietic level. (C) 2000 by The American Society of Hematology.

关键词：血细胞生成/药物作用血细胞生成/生理学造血干细胞/细胞学造血干细胞/药物作用造血干细胞/生理学 Ikaros转录因子白细胞介素类/药理学淋巴细胞/细胞学淋巴细胞/药物作用淋巴细胞/生理学巨噬细胞/细胞学单核细胞/细胞学脑啡肽酶/分析原癌基因蛋白质类/遗传学受体蛋白质酪氨酸激酶类/遗传学受体细胞表面/遗传学重组蛋白质类/代谢重组蛋白质类/药理学逆转录聚合酶链反应信号传导干细胞因子/药理学 T淋巴细胞/细胞学 T淋巴细胞/药物作用 T淋巴细胞/生理学转录因子/遗传学转录因子/代谢肿瘤坏死因子α/药理学锌指 fms样酪氨酸激酶3 人类

来源：评论

学校读者我要写书评

暂无评论

The hemoregulatory peptide pEEDCK may inhibit stem cell proliferation via hydropathic binding to antisense sequence motifs in interleukin-11 and other growth factors

引用

MOLECULAR PHARMACOLOGY 1999年第4期56卷 665-674页

作者： Paukovits, JB Rutter, R Ganglberger, E Karlic, HI Marian, B Paukovits, WR Univ Vienna Inst Tumor Biol Canc Res A-1090 Vienna Austria Hanuschspital Boltzmann Inst Leukemia Res Vienna Austria

In undisturbed bone marrow, most hemopoietic stem cells are nonproliferating despite the presence of multiple growth factors. Endogenous inhibitory factors are responsible for maintenance of this quiescence. Previously we sequenced and synthesized the inhibitory pentapeptide pGlu-Glu-Asp-Cys-Lys (pEEDCK), which originally derives from granulocytes, and investigated the role of this peptide in stem cell quiescence. To provide some mechanistic insight, in the present work we studied the structural relationship of this peptide to specific growth-factor-derived sequence motifs. In the murine system in vivo as well as in long-term bone marrow, antiserum to pEEDCK produced a significant stimulation of formation of colony-forming units-granulocyte/macrophage. Binding of peptides to proteins often takes place at hydropathically complementary sites.

关键词：抗体/药理学结合部位骨髓细胞/药物作用细胞黏附/药物作用细胞分裂细胞培养的粒细胞/细胞学粒细胞/药物作用生长抑制物/免疫学生长抑制物/药理学造血干细胞/细胞学造血干细胞/药物作用免疫法白细胞介素11/代谢巨噬细胞/细胞学巨噬细胞/药物作用小鼠近交BALBC 寡肽类/免疫学寡肽类/药理学肽类/药理学吡咯烷酮羧酸/类似物和衍生物干细胞/药物作用动物女(雌)性小鼠

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：