A novel technique has been developed for selecting non-chemotactic mutants of Salmonella typhimurium that are defective in transmission of information from the chemoreceptor to the flagella. This technique utilizes a ...
详细信息
A novel technique has been developed for selecting non-chemotactic mutants of Salmonella typhimurium that are defective in transmission of information from the chemoreceptor to the flagella. This technique utilizes a preformed gradient in a vertical column of liquid medium, which separates wild types, non-motile and motile but non-chemotactic into three regions. In the first application of the technique, 71 such mutants were obtained, of which 67 exhibited smooth motility and four had unco-ordinated motility. Complementation analysis, done by abortive transduction of recombination-defective recipients, indicates that there may be as many as six genetically distinct classes of che mutants in Salmonella .
暂无评论