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Evaluation of the gel system for abo grouping and D typing

TRANSFUSION 1999年第3期39卷 300-305页

作者： Langston, MM Procter, JL Cipolone, KM Stroncek, DF NIH Warren G Magnuson Clin Ctr Dept Transfus Med Bethesda MD 20892 USA

BACKGROUND: The gel agglutination assay has been approved by the Food and Drug Administration as an alternative to the tube assay for the detection of red cell antibodies. It has also been approved recently by the Food and Drug Administration for abo blood grouping and D typing. STUDY DESIGN AND METHODS: Tube and gel agglutination assays were compared for abo grouping and D typing of 100 donor and 100 patient specimens. abo grouping of 14 specimens of known abo groups and D typing of 10 specimens with weak D were also compared. When antigen typing or isohemagglutinin results differed, gel testing was repeated by the use of modified incubation times, reagent or specimen volumes, and red cell concentrations. RESULTS: abo grouping and D typing in all patient and donor specimens concurred. B isohemagglutinins were not detected in seven group A specimens. Six of seven discrepancies were resolved when gel tests were incubated at room temperature with increased serum or plasma volume. Weak D was detected in all 10 specimens tested by both assays. When weak A and/or B were tested with monoclonal antibody reagents, the correct phenotypes were identified in 9 specimens by gel assay and in 10 by tube assay. Using human antisera, 6 specimens were correctly phenotyped by gel assay and 7 by tube assay. CONCLUSION: The gel assay performed as well as the tube assay in detection of A, B, and D, but the tube assay was slightly better at detecting B isohemagglutinins. The gel assay can be used in place of the tube assay far abo blood grouping and D typing.

关键词： abo血型系统/免疫学抗体特异性抗凝药血型鉴定和交叉配血/方法螯合剂依地酸评价研究(主题) 凝胶类血凝集试验/方法血凝素类/血液血凝素类/免疫学同种抗体/血液肿瘤/血液肿瘤/免疫学 Rh-Hr血型系统/免疫学 Rho(D)免疫球蛋白敏感性与特异性温度青少年成年人老年人老年人 80以上儿童儿童学龄前女(雌)性人类男(雄)性中年人

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STUDIES ON IGA CLASS CIRCULATORY RED-CELL ANTIBODIES .2. PROPERTIES OF IGA CLASS ANTI-A, ANTI-B

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TRANSFUSION SCIENCE 1995年第2期16卷 161-166页

作者： BOOTH, JR SOKOL, RJ Trent Blood Transfusion Centre Sheffield U.K.

The properties of IgA class red cell alloantibodies were determined using an anti-A, anti-B preparation confirmed to be free from IgG and IgM. The following characteristics were found: a thermal optimum of 37 degrees;saline agglutination enhanced by anti-human IgA;activity substantially unchanged after 1 year's storage at 4 degrees C, but reduced by papain and destroyed by pepsin and by reduction and alkylation;neutralization by AB blood group substance;an inability to activate complement;and a distinctive pattern of apparent molecular sizes (reflecting dimeric and monomeric forms and complexes with serum proteins) which was significantly altered by the action of enzymes and by reduction and alkylation.

关键词： abo血型系统/免疫学红细胞/免疫学免疫球蛋白A/血液同种抗体/血液同种抗体/分类人类

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STUDIES ON IGA CLASS CIRCULATORY RED-CELL ANTIBODIES .1. PREPARATION AND IDENTIFICATION OF IGA CLASS ANTI-A, ANTI-B

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TRANSFUSION SCIENCE 1995年第2期16卷 155-160页

作者： BOOTH, JR SOKOL, RJ Trent Blood Transfusion Centre Sheffield U.K.

To elucidate the role of IgA class alloantibodies in transfusion practice, anti-A, anti-B was prepared using pooled serum from healthy group O donors. IgG was removed by protein A and protein G, whilst IgM was extracted by concanavalin A and anti-human mu chain. The final preparation contained both IgA1 and IgA2;it had an IgA concentration similar to the original serum pool and an environment as near to normal as possible;the absence of IgG and IgM was confirmed by sensitive enzyme-linked antiglobulin tests, and extensive haemagglutination studies showed that anti-A, anti-B was the only red cell anti-body present.

关键词： abo血型系统/免疫学红细胞/免疫学免疫球蛋白A/血液同种抗体/血液同种抗体/分类人类

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Removal of anti-A/B antibodies for successful kidney transplantation between abo blood type incompatible couples

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TRANSFUSION SCIENCE 1996年第3期17卷 455-462页

作者： Tanabe, K Takahashi, K Agishi, T Toma, H Ota, K Department of Urology Tokyo Women's Medical College Japan.

A growing shortage of cadaveric donors has prompted expansion of the criteria for acceptable living donors. Because of this, abo-incompatible kidney transplantation has been carried out. To remove anti-A and/or anti-B antibodies, the recipients received one or two sessions of double filtration plasmapheresis (DFPP) and three or four sessions of immunoadsorption prior to transplantation until the anti-A IgG/IgM titers and/or anti-B IgG/IgM titers decreased to 1:16 or less. Our immunosuppressive protocol involved treatment with the drugs methylprednisolone, cyclosporine, azathioprine, anti lymphocyte globulin and deoxyspergualin. The patient survival was 98% at 1 month, 98% at 3 months, 94% at 6 months, and 92% at 1-5 years. Graft survival was 92% at 1 month, 88% at 3 months, 85% at 6 months, 81% at 1 year, and 76% at 5 years. Both DFPP and/or immunoadsorption eliminated anti-abo antibodies from abo-incompatible kidney transplant recipients effectively and safely. The results of the abo-incompatible kidney transplantation were acceptable and not different from those of abo-compatible cases. Copyright (C) 1996 Elsevier Science Ltd

关键词： abo血型系统/免疫学抗体/血液血型不合血型鉴定和交叉配血移植物排斥/血液移植物排斥/免疫学移植物排斥/预防和控制免疫吸附技术肾移植肾透析人类

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Hypercoagulability state in hip and knee surgery:: influence of abo antigenic system and allogenic transfusion

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TRANSFUSION SCIENCE 1999年第1期20卷 17-20页

作者： Iturbe, T Cornudella, R de Miguel, R Olave, T Moreno, A Callén, L Gutiérrez, M Hosp Clin Univ Dept Hematol Zaragoza 50009 Spain

It is well know that people with blood group O have a lower risk for venous thromboembolic disease (VTED) than does the general population. Moreover blood transfusion has been identified as a risk factor for VTED in patients with major trauma. The aim of this work is to investigate the behaviour of several markers of hypercoagulability before and after substitutive surgery of hip and knee in order to evaluate the relationship between the plasmatic levels of these markers, the abo antigenic system and the allogenic blood transfusion. The plasmatic levels of D dimer (D-D), thrombin - antithrombin complex (TAT), and fragment 1 + 2 of prothrombin (F1 + 2) have been determined by the ELISA method in 79 patients subject to substitutive surgery of hip or knee one day before and one day after surgery. The 41 patients with blood groups different from O had presurgical levels of Fl + 2 higher than those of group O (p = 0.004), while no differences could be found for D-D and TAT. The 28 patients who received allogenic blood presented higher levels of D-D one day after surgery than non-transfused patients (p = 0.043);the practice of transfusion did not modify significantly the levels of TAT and Fl + 2 after surgery. In accordance with these results we suggest that blood group and transfusion are risk factors for hypercoagulability, and therefore we advise for a restrictive policy of transfusion practice. New therapies such as aprotinin should be assayed in order to minimize blood loss. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.

关键词： abo血型系统/免疫学关节成形术置换髋关节成形术置换膝输血同种抗原/血液回顾性研究血栓形成倾向/免疫学移植同种成年人老年人老年人 80以上女(雌)性人类男(雄)性中年人

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Production, crystallization and diffraction to atomic resolution of an antibody Fv specific for the blood-group A oligosaccharide antigen

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ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 1998年第6-2期54卷 1456-1459页

作者： Patenaude, SI MacKenzie, CR Bilous, D To, RJ Ryan, SE Young, NM Evans, SV Univ Ottawa Dept Biochem Ottawa ON K1H 8M5 Canada Natl Res Council Canada Inst Biol Sci Ottawa ON K1A 0R6 Canada

The histoblood-group abo carbohydrate antigens are well known as important factors in blood transfusions, but they can also act as receptors for infectious agents and have been implicated in susceptibility to certain carcinomas. A single-chain variable-domain antigen-binding fragment (scFv) gene based on the known sequence of an anti-blood-group-A monoclonal antibody (AC1001) has been synthesized and expressed in Escherichia coti. The purified scFv preparation existed primarily in the monomeric form but also contained large amounts of dimeric and higher oligomeric forms. The corresponding variable-domain antigen-binding fragment (Fv) was generated by cleaving the V-L-V-H linker with subtilisin, and its activity was demonstrated by surface plasmon resonance with an immobilized bovine serum albumin A-trisaccharide conjugate (K-D = 290 mu M). AC1001 Fv crystals grown in the presence of N-acetylgalactosamine diffracted to 0.93 Angstrom resolution. This is the first reported example of a crystal of an antibody antigen-binding fragment diffracting to atomic resolution.