Human palatine secretion contains blood-group substances in high concentration, but the viscous substances have been difficult to fractionate without pretreatment to render them water-soluble. We show that it is possi...
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Human palatine secretion contains blood-group substances in high concentration, but the viscous substances have been difficult to fractionate without pretreatment to render them water-soluble. We show that it is possible to fractionate human palatine secretion with retention of the viscous properties of the blood group ***-stimulated human palatine secretion from secretors of blood group A was subjected to isoelectric fractionation on a polytetrafluoroethylene-coated column. Blood-group substance activity was recovered in an acidic (pI < 3), a neutral (pI 6–7) and an alkaline (pI > 10) component. Virus-haemagglutination inhibition activity was found in the acidic and neutral components. The neutral component was the largest and retained the gel-like properties of the original secretion. Boiling of aliquots did not appear to affect the acidic and alkaline components. In the neutral component, an increase in the titres of blood-group substance activity and virus-haemagglutination inhibition activity was observed, concomitant with loss of viscous properties and change in electrophoretic *** acid analysis did not reveal any significant differences between the acidic and neutral components. The alkaline one was different from the two others. It appeared possible that the water-soluble acidic and alkaline components were derived from the viscous, neutral component.
A case of delayed hemolytic transfusion reaction in a 43-year-old patient is presented. Pretransfusion tests revealed no antibody, but tests performed when the reaction occurred demonstrated four. Ten months and 15 mo...
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A case of delayed hemolytic transfusion reaction in a 43-year-old patient is presented. Pretransfusion tests revealed no antibody, but tests performed when the reaction occurred demonstrated four. Ten months and 15 months later, when the patient's serum was again tested, the antibodies were undetectable. The significance of such antibodies is discussed.
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