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The bHLH Transcription Factor PubHLH66 Improves Salt Tolerance in Daqing Poplar (Populus ussuriensis)

FORESTS 2024年第11期15卷 2051-2051页

作者： Li, Dandan Wang, Jindan Pan, Yuxin Wang, Hui Dang, Xinyao Zhao, Shihao Wang, Yucheng Shenyang Agr Univ Coll Forestry Shenyang 110866 Peoples R China Key Lab Forest Tree Genet Breeding & Cultivat Liao Shenyang 110866 Peoples R China

Elevated salinity negatively impacts plant growth and yield, presenting substantial challenges to agricultural and forestry productivity. The bHLH transcription factor family is vital for plants to cope with various abiotic stresses. However, it remains uncertain whether bHLH transcription factors can regulate salt stress in Populus ussuriensis. In the following study, a salt-induced bHLH transcription factor PubHLH66 was identified from P. ussuriensis. PubHLH66 has a typical and conserved bHLH domain. Subcellular localization and yeast two-hybrid (Y2H) assays confirmed that it is a nucleus-localized transactivator and the activation region is located at the N-terminus. PubHLH66-OE and PubHLH66-SRDX transgenic P. ussuriensis were obtained through Agrobacterium-mediated leaf disc transformation. Morphological and physiological results demonstrated that PubHLH66-OE enhanced salinity tolerance, as indicated by reduced electrolyte leakage (EL), malondialdehyde (MDA), and H2O2 levels, along with increased proline contents and activities of peroxidase (POD) and superoxide dismutase (SOD). In contrast, PuHLH66-SRDX poplar showed decreased salt tolerance. Quantitative real-time PCR (RT-qPCR) confirmed that PubHLH66 enhanced salt tolerance by regulating the expression of genes such as PuSOD, PuPOD, and PuP5CS, resulting in reduced reactive oxygen species (ROS) accumulation and an improved osmotic potential. Thus, PubHLH66 could be a candidate gene for molecular breeding to enhance salt tolerance in plants. These results laid a foundation for exploring the mechanisms of salt tolerance in P. ussuriensis, facilitating the development of more salt-tolerant trees to combat the increasing issue of soil salinization globally.

关键词： salt resistance basic helix-loop-helix gene function reactive oxygen species (ROS) scavenging proline

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The bHLH transcription factor GhPAS1 mediates BR signaling to regulate plant development and architecture in cotton

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The Crop Journal 2021年第5期9卷 1049-1059页

作者： Huanhuan Wu Zhongying Ren Lei Zheng Mengzhen Guo Jingyu Yang Liyong Hou Ghulam Qanmber Fuguang Li Zuoren Yang State Key Laboratory of Cotton Biology Cotton Research Institute of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001HenanChina

Cotton (Gossypium spp.) is the most important natural textile fiber crop in the *** ideal plant architecture of cotton is suitable for mechanical harvesting and productivity in modern agricultural ***,cotton genes regulating plant development and architecture have not been fully *** identified a basic helix-loop-helix (b HLH) transcription factor,GhPAS1 (PAGODA1 SUPPRESSOR1) in *** (Upland cotton).GhPAS1 was located in the nucleus and showed a strong transcription activation ***-specific expression patterns showed that GhPAS1 was highly expressed in floral organs,followed by high expression in early stages of ovule development and rapid fiber ***1 overexpression in Arabidopsis and BRZ (brassinazole,BR biosynthesis inhibitor) treatment indicated that GhPAS1 positively regulates and responds to the BR (brassinosteroid) signaling pathway and promotes cell ***1 overexpression in Arabidopsis mediated plant development in addition to increasing plant ***-induced gene silencing of GhPAS1 indicated that down-regulation of GhPAS1 inhibited cotton growth and development,as plant height,fruit branch length,and boll size of silenced plants were lower than in control *** length and seed yield were also lower in silenced *** conclude that GhPAS1,a b HLH transcription factor,regulates plant development and architecture in *** findings may help breeders and researchers develop cotton cultivars with desirable agronomic characteristics.

关键词： Cotton fiber basic helix-loop-helix GhPAS1 Plant architecture Virus induced gene silencing

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Transcriptional integration of plant responses to iron availability

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JOURNAL OF EXPERIMENTAL BOTANY 2021年第6期72卷 2056-2070页

作者： Gao, Fei Dubos, Christian Univ Montpellier Inst Agro INRAE CNRSBPMP Montpellier France

Iron is one of the most important micronutrients for plant growth and development. It functions as the enzyme cofactor or component of electron transport chains in various vital metabolic processes, including photosynthesis, respiration, and amino acid biosynthesis. To maintain iron homeostasis, and therefore prevent any deficiency or excess that could be detrimental, plants have evolved complex transcriptional regulatory networks to tightly control iron uptake, translocation, assimilation, and storage. These regulatory networks are composed of various transcription factors;among them, members of the basic helix-loop-helix (bHLH) family play an essential role. Here, we first review recent advances in understanding the roles of bHLH transcription factors involved in the regulatory cascade controlling iron homeostasis in the model plant Arabidopsis, and extend this understanding to rice and other plant species. The importance of other classes of transcription factors will also be discussed. Second, we elaborate on the post-translational mechanisms involved in the regulation of these regulatory networks. Finally, we provide some perspectives on future research that should be conducted in order to precisely understand how plants control the homeostasis of this micronutrient.

关键词： Arabidopsis thaliana basic helix-loop-helix bHLH iron homeostasis rice transcription factor

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Genome-Wide Identification and Expression Profiling of the bHLH Transcription Factor Gene Family in Saccharum spontaneum Under Bacterial Pathogen Stimuli

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TROPICAL PLANT BIOLOGY 2021年第3期14卷 283-294页

作者： Ali, Ahmad Javed, Talha Zaheer, Uroosa Zhou, Jing-Ru Huang, Mei-Ting Fu, Hua-Ying Gao, San-Ji Fujian Agr & Forestry Univ Natl Engn Res Ctr Sugarcane Fuzhou 350002 Fujian Peoples R China

The basic helix-loop-helix (bHLH) families that act as transcription factors play a central role in regulating a wide range of physiological, metabolic, and developmental processes in higher organisms. Systematic identification and characterization of bHLH gene families has not yet been performed in sugarcane. In the present study, 37 SsbHLH genes (101 total alleles) were identified in Saccharum spontaneum (Ss), with 1-4 alleles each. Phylogenetic analysis indicated that these SsbHLH could be classified into 17 subfamilies according to bHLH domains. All of the SsbHLHs were localized on 20 chromosomes, and the SsbHLH member's likely arose through gene duplication. Based on a sugarcane transcriptome dataset trigged by Acidovorax avenae subsp. avenae (Aaa) causing red stripe, SsbHLH27 and SsbHLH36 were significantly upregulated by 1.12- to 5.96-fold [log(2)(Fold Change)] in cultivar ROC22 (red stripe resistant), while both genes were significantly depressed in cultivar MT11-610 (red stripe susceptible) under Aaa infection. Based on another sugarcane transcriptome dataset trigged by Xanthomonas albilineans (Xa) causing leaf scald, four genes (SsbHLH03/04/36/37) were highly upregulated by 1.23- to 5.11-fold [log(2)(Fold Change)] in LCP85-384 (leaf scald-resistant), but downregulated or non-significantly changed in cultivar ROC20 (leaf scald-susceptible) under Xa infection. SsbHLH15/17 were positively induced by both Aaa and Xa stimuli, whereas SsbHLH30/33 were negatively regulated by both pathogens. Some genes, such as SsbHLH06/11, had different expression profiles under the two pathogens infections, showing increased expression after Aaa infection but reduced expression with Xa. These findings provide important candidate genes for further functional analyses in sugarcane in response to bacterial stress.

关键词： Sugarcane basic helix-loop-helix Expression analysis Biotic stress Transcriptome

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Genome-wide investigation of bHLH genes and expression analysis under salt and hormonal treatments in Andrographis paniculata

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INDUSTRIAL CROPS AND PRODUCTS 2022年 183卷

作者： Xu, Jiahao Xu, Hongliang Zhao, Hongguang Liu, Hui Xu, Ling Liang, Zongsuo Zhejiang Sci Tech Univ Coll Life Sci & Med Key Lab Plant Secondary Metab & Regulat Zhejiang P Hangzhou 310018 Peoples R China Tianjin Tasly modern TCM Resources Co Ltd Tianjin 300410 Peoples R China Tianjin Key Lab Modern Chinese Med Resources Res E Tianjin 300410 Peoples R China Shaanxi Tasly Plant Pharmaceut Co Ltd Shaanxi 726000 Peoples R China Univ Western Australia UWA Inst Agr Fac Sci UWA Sch Agr & Environm Perth WA 6009 Australia

Andrographis paniculata, a traditional bulk herb known as a natural antibiotic, is widely cultivated in tropical and subtropical regions. The basic helix-loop-helix (bHLH) transcription factors (TFs) act in a variety of physiological processes in plants, such as plant development, secondary metabolism and abiotic stress responses. There has been no report so far in the research on the bHLH gene family in A. paniculata. In our study, genome-wide analysis was performed for the A. paniculata bHLH (ApbHLH) gene family, and a total of 122 ApbHLH genes (ApbHLHs) were identified, which were renamed on the basis of their physical locations on the chromosomes and the contig numbers. They were classified into 26 subfamilies through phylogenetic analysis, validated by analyses of conserved motifs and gene structures. Subsequently, chromosome distribution, cis-regulatory elements (CREs), syntenic analysis and gene expression profiling were undertaken. The ApbHLHs were randomly distributed on 24 chromosomes, of which three ApbHLHs were localized on unassembled scaffolds. Gene duplication events indicated that ApbHLHs were mainly subject to strong purifying selection during evolution and were affinely closer to dicotyledons, especially Sesamum indicum and Solanum lycopersicum. In addition, the qRT-PCR results revealed that ApbHLHs had specific expression profiles under salinity, salicylic acid (SA) and 6benzylaminopurine (6-BA) treatments, especially ApbHLH47 and ApbHLH109, which were significantly down regulated under all three treatments. It implies that they have essential functions in response to abiotic stresses and plant development, and thus can be considered as candidate regulatory factors for further study.

关键词： Andrographis paniculata Abiotic stress basic helix-loop-helix Evolution Expression profile

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Cryptochrome 1 Inhibits Shoot Branching by Repressing the Self-Activated Transciption loop of PIF4 in Arabidopsis

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Plant Communications 2020年第3期1卷 98-106页

作者： Huawei Zhai Lu Xiong Hongyu Li Xiangguang Lyu Guodong Yang Tao Zhao Jun Liu Bin Liu The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI) Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingP.R.China State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandongP.R.China

Cryptochrome 1(CRY1)is an important light receptor essential for de-etiolation of Arabidopsis ***,its function in regulating plant architecture remains ***,we show that mutation in CRY1 resulted in increased branching of Arabidopsis *** investigate the underlying mechanism,we analyzed the expression profiles of branching-related genes and found that the mRNA levels of Phytochrome Interaction Factor 4(PIF4)and PIF5 are significantly increased in the cry1 *** analysis showed that the pif4 or pif4pif5 mutant is epistatic to the cry1 mutant,and overexpression of PIF4 conferred increased ***,we demonstrated that PIF4 proteins physically associate with the G-box motif within the PIF4 promoter to form a self-activated transcriptional feedback loop,while CRY1 represses this process in response to blue *** together,this study suggests that the CRY1–PIF4 module regulates gene expression via forming a regulatory loop and shoot branching in response to ambient light conditions.

关键词： cryptochrome PIF branching basic helix-loop-helix photoreceptor Arabidopsis

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Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana

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PLANTA 2020年第3期251卷 61-61页

作者： Nuraini, Latifa Ando, Yukiko Kawai, Kentaro Tatsuzawa, Fumi Tanaka, Kotomi Ochiai, Masaki Suzuki, Katsumi Aragones, Veronica Daros, Jose-Antonio Nakatsuka, Takashi Gifu Univ United Grad Sch Agr Sci 1-1 Yanagido Gifu Gifu 5011193 Japan Shizuoka Univ Grad Sch Integrated Sci & Technol Suruga Ku 836 Ohya Shizuoka 4228529 Japan Shizuoka Univ Fac Agr Suruga Ku 836 Ohya Shizuoka 4228529 Japan Iwate Univ Fac Agr 3-18-8 Ueda Morioka Iwate 0208550 Japan Gifu Univ Fac Appl Biol Sci Yanagido 1-1 Gifu 5011193 Japan Shizuoka Univ Coll Agr Acad Inst Suruga Ku 836 Ohya Shizuoka 4228529 Japan Univ Politecn Valencia Inst Biol Mol & Celular Plantas CSIR E-46022 Valencia Spain

Main conclusion MiMYB1 and MibHLH2 play key roles in anthocyanin biosynthesis in Matthiola incana flowers. We established a transient expression system using Turnip mosaic virus vector in M. incana. Garden stock (Matthiola incana (L.) R. Br.) is a popular flowering plant observed from winter to spring in Japan. Here we observed that anthocyanin accumulation in 'Vintage Lavender' increased with flower development, whereas flavonol accumulation remained constant throughout flower development. We obtained five transcription factor genes, MiMYB1, MibHLH1, MibHLH2, MiWDR1, and MiWDR2, from M. incana floral cDNA contigs. Yeast two-hybrid analyses revealed that MiMYB1 interacted with MibHLH1, MibHLH2, and MiWDR1, but MiWDR2 did not interact with any transcription factor. Expression levels of MiMYB1 and MibHLH2 increased in petals during floral bud development. Their expression profiles correlated well with the temporal profiles of MiF3MODIFIER LETTER PRIMEH, MiDFR, MiANS, and Mi3GT transcripts and anthocyanin accumulation profile. On the other hand, MibHLH1 was expressed weakly in all organs of 'Vintage Lavender'. However, high expression levels of MibHLH1 were detected in petals of other cultivars with higher levels of anthocyanin accumulation than 'Vintage Lavender'. MiWDR1 and MiWDR2 maintained constant expression levels in petals during flower development and vegetative organs. Transient MiMYB1 expression in 1-month-old M. incana seedlings using a Turnip mosaic virus vector activated transcription of the endogenous anthocyanin biosynthetic genes MiF3MODIFIER LETTER PRIMEH, MiDFR, and MiANS and induced ectopic anthocyanin accumulation in leaves. Therefore, MiMYB1 possibly interacts with MibHLH2 and MiWDR1, and this trimeric protein complex activates the transcription of anthocyanin biosynthetic genes in M. incana flowers. Moreover, MibHLH1 acts as an enhancer of anthocyanin biosynthesis with the MiMYB1-MibHLH2-MiWDR1 complex. This study revealed the molecular mechanism

关键词： basic helix-loop-helix Garden stock Turnip mosaic virus WD40 repeat

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The bHLH protein OsIRO3 is critical for plant survival and iron (Fe) homeostasis in rice (Oryza sativa L.) under Fe-deficient conditions

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SOIL SCIENCE AND PLANT NUTRITION 2020年第4期66卷 579-592页

作者： Wang, Fan Itai, Reiko N. Nozoye, Tomoko Kobayashi, Takanori Nishizawa, Naoko K. Nakanishi, Hiromi Univ Tokyo Grad Sch Agr & Life Sci Dept Global Agr Sci Tokyo 1138657 Japan Meiji Gakuin Univ Ctr Liberal Arts Tokyo Japan Ishikawa Prefectural Univ Res Inst Bioresources & Biotechnol Nonoichi Ishikawa Japan

Iron (Fe) is an essential element for most organisms since it is involved in various biological reactions. Fe deficiency is a major constraint for plant growth and leads to low crop yields and poor nutritional quality. Fe uptake and translocation in plants require the coordinated expression of multiple genes, which is regulated by various transcription factors (TFs), especially members of the basic helix-loop-helix (bHLH) family. OsIRO3 was first reported as a repressor related to Fe homeostasis gene regulation in roots based on evidence in overexpressing rice. However, its functions in regulating rice growth and Fe homeostasis between roots and shoots during Fe deficiency is poorly understood. Here, we developed osiro3 knockout mutants by the CRISPR/Cas9 system. The leaves of osiro3 knockout mutants showed abnormal growth and development after Fe-deficiency treatment. Strong lesions occurred on the young leaves of osiro3 knockout mutants, which even led to plant death during severe Fe deficiency. Furthermore, accumulation of reactive oxygen species (ROS), Fe and manganese concentrations were all elevated in the shoots of osiro3 knockout mutants under the Fe deficiency stress. Quantitative RT-PCR showed that OsNAC4 for ROS induced plant hypersensitive cell death and OsFerritins for Fe storage and overload were all significantly activated in osiro3 knockout shoots after Fe deficiency treatment. In osiro3 knockout mutants, Fe-deficiency response genes were induced in roots, but suppressed in shoots, and furthermore Fe sufficiency/overload genes were induced in shoots under Fe-deficient conditions according to DNA microarray. Yeast two-hybrid analysis revealed a positive interaction between OsIRO3 and the homologous protein OsbHLH062, which was also transcriptionally increased in Fe-deficient roots. OsIRO3 is essential for maintaining plant survival in rice under Fe-deficient conditions and plays an important role in signal transmission from shoots to roots which is cr

关键词： Fe deficiency Fe sufficiency transcription factor basic helix-loop-helix signal transmission

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Phylogenetic analysis of achaete-scute complex genes in metazoans

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MOLECULAR GENETICS AND GENOMICS 2020年第3期295卷 591-606页

作者： Wang, Yong Wang, Guang-Dong He, Qin-Liu Luo, Zhi-Peng Yang, Liu Yao, Qin Chen, Ke-Ping Jiangsu Univ Sch Food & Biol Engn 301 Xuefu Rd Zhenjiang 212013 Jiangsu Peoples R China Jiangsu Univ Inst Life Sci 301 Xuefu Rd Zhenjiang 212013 Jiangsu Peoples R China

Achaete-scute complex (ASC) genes play essential roles in regulating neurogenesis of metazoans. Various metazoan species have greatly different numbers of genes in ASCa, ASCb and ASCc families. To explore evolutionary mechanisms of metazoan ASC genes, Blast (basic local alignment search tool) searches and phylogenetic analyses were conducted to identify ASC genes in metazoan species and to infer phylogenetic relationship between various ASC genes. As a result, 2784 ASC genes were identified in 804 metazoan species. The phylogenetic tree constructed using 1237 unique bHLH motifs shows that metazoan ASCa, ASCb and ASCc families contain six (a1-a6), five (b1-b5) and three (c1-c3) bHLH genes, respectively. Further phylogenetic analyses suggest that ASC genes in metazoans are derived from a primitive c gene, those in insects are derived from c2 gene, and those in chordates are derived from a2 and a3 genes. Data of gene linkage demonstrate that insect a6 is derived from a4 but not from a5, and chordate a2 is ancestral to b5 only, whilst a3 is ancestral to both b3 and b5. It is concluded that current ASC gene families in metazoans were established through a series of sub- and/or neo-functionalization to duplicated ancestral ASC gene(s). These results provide good references for exploring evolutionary mechanisms of other bHLH genes in metazoans. Besides, gene subtyping is considered as an efficient method for evolutionary studies on closely related homologous genes.

关键词： Development Evolution Gene subtype basic helix-loop-helix Duplication

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Further insights into the role of bHLH121 in the regulation of iron homeostasis in Arabidopsis thaliana

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PLANT SIGNALING & BEHAVIOR 2020年第10期15卷 1795582-1795582页

作者： Gao, Fei Robe, Kevin Dubos, Christian Univ Montpellier BPMP CNRS INRAEInst Agro Montpellier France

Iron (Fe) is an important micronutrient for plant growth and development but any excess of Fe is toxic because of the Fe-dependent generation of reactive oxygen species (ROS). Thus, Fe homeostasis must be tightly regulated. In Arabidopsis thaliana, a cascade of transcription factors has been identified as involved in the regulation of this process by modulating the expression of genes related to Fe uptake, transport, and storage. Recently, it was demonstrated that in response to Fe deficiency, bHLH121/URI (UPSTREAM REGULATOR OF IRT1) directly activates the expression of several genes involved in this regulatory network. It was also shown that bHLH121 interacts with ILR3 (bHLH105) and its homologs. Herein it is shown that bHLH121 is necessary for the expression of the main markers of the plant responses to Fe excess, the ferritin genes (i.e. FER1, FER3, and FER4). bHLH121 regulates ferritin genes expression by directly binding to their promoters, at the same locus than the ILR3-PYE repressive complex. Therefore, this study highlight that bHLH121, PYE, and ILR3 form a chain of antagonistic switches that regulate the expression of ferritin genes. The implication of this finding is discussed.

关键词： Arabidopsis thaliana basic helix-loop-helix bHLH47 bHLH105 bHLH121 ferritins ILR3 iron homeostasis PYE transcription factor

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