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Development of conductometric biosensor array for simultaneous determination of maltose, lactose, sucrose and glucose

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TALANTA 2013年 115卷 200-207页

作者： Soldatkin, O. O. Peshkova, V. M. Saiapina, O. Y. Kucherenko, I. S. Dudchenko, O. Y. Melnyk, V. G. Vasylenko, O. D. Semenycheva, L. M. Soldatkin, A. P. Dzyadevych, S. V. Natl Acad Sci Ukraine Inst Mol Biol & Genet Lab Biomol Elect UA-03143 Kiev Ukraine Taras Shevchenko Natl Univ Kyiv Inst High Technol UA-01003 Kiev Ukraine Natl Acad Sci Ukraine Inst Electrodynam Dept Elect & Magnet Measurements UA-03680 Kiev Ukraine

The aim of this work was to develop an array of biosensors for simultaneous determination of four carbohydrates in solution. Several enzyme systems selective to lactose, maltose, sucrose and glucose were immobilised on the surface of four conductometric transducers and served as bio-recognition elements of the biosensor array. Direct enzyme analysis carried out by the developed biosensors was highly sensitive to the corresponding substrates. The analysis lasted 2 min. The dynamic range of substrate determination extended from 0.001 mM to 1.0-3.0 mM, and strongly depended on the enzyme system used. An effect of the solution pH, ionic strength and buffer capacity on the biosensors responses was investigated;the conditions of simultaneous operation of all biosensors were optimised. The data on cross-impact of the substrates of all biosensors were obtained;the biosensor selectivity towards possible interfering carbohydrates was tested. The developed biosensor array showed good signal reproducibility and storage stability. The biosensor array is suited for simultaneous, quick, simple, and selective determination of maltose, lactose, sucrose and glucose. (C) 2013 Elsevier B.V. All rights reserved.

关键词： Conductometric transducer biosensor array Sucrose Glucose Maltose Lactose

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Electrochemical biosensor array for liver diagnosis using silanization technique on nanoporous silicon electrode

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JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2007年第1期103卷 32-37页

作者： Song, Min-Jung Yun, Dong-Hwa Min, Nam-Ki Hong, Suk-In Korea Univ Dept Chem & Biol Engn Seoul 136701 South Korea Korea Univ Dept Control & Instrumentat Engn Yeongi 339700 Choongnam South Korea

An electrochemical biosensor array system was fabricated for the diagnosis and monitoring of liver diseases. Analysis on this array system with multiple samples was performed for point-of-care testing or home-use applications. Cholesterol, bilirubin and aminotransferases present in the serum are well-known biomarkers for liver diseases. For this study, we describe our biosensor array system consisting of cholesterol, bilirubin and glutamate sensors. To immobilize sensing enzymes on the array system, we employed a silanization technique. We observed that porous silicon layers formed on each working electrode notably increase the effective surface area. Sensing electrodes were placed in sampling wells to minimize the cross-interference effect so that multiple sampling would be possible with a low noise current. Compared with traditional analyte measurement procedures, our novel analytical device demonstrated acceptable sensitivities for the analyses of multiple samples and analytes without a marked cross-interference effect. The device sensitivities observed were 0.2656 mu A/mM for cholesterol, 0.15354 mA/mM for bilirubin, 0.13698 mu A/(U/l) for alanine aminotransferase (ALT) and 0.45439 mu A/(U/l) for aspartate aminotransferase (AST).

关键词： cholesterol sensor bilirubin sensor glutamate sensor alanine aminotransferase (ALT)/aspartate aminotransferase (AST) activities biosensor array porous silicon electrochemical biosensor

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An Electrochemical biosensor array for Rapid Detection of Alanine Aminotransferase and Aspartate Aminotransferase

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BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2009年第3期73卷 474-478页

作者： Song, Min-Jung Yun, Dong-Hwa Hong, Suk-In Korea Univ Dept Chem & Biol Engn Seoul 136701 South Korea Korea Univ Sch Elect Engn Seoul 136701 South Korea

An increment of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) in human serum indicates an abnormal symptom of the liver. Hence, an electrochemical biosensor array that uses micro electromechanical systems technology is required for rapid and integrated measurement of ALT/AST. Here we describe a biosensor array consisting of two glutamate sensors. It turned out that porous silicon layers formed on each working electrode were useful to increase the effective surface area. This biosensor array was constructed with platinum electrodes and a polydimethylsiloxane (PDMS) microchannel. Electrodes in sampling wells minimized a cross-interference effect and permitted multiple sampling by immobilization with glutamate oxidase using a silanization technique. The device sensitivities derived from semi-logarithmic plots were 0.145 mu A/(U/l) for ALT and 0.463 mu A/(U/l) for AST over a range of 1.3 U/l to 250 U/l. Hene, this ALT/AST biosensor array can be applied in diagnostic and home use.

关键词： glutamate sensor alanine aminotransferase (ALT)/aspartate aminotraseferase (AST) activities biosensor array porous silicon electrochemical biosensor

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Machine learning linked evolutionary biosensor array for highly sensitive and specific molecular identification

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biosensorS & BIOELECTRONICS 2020年 170卷 112670-112670页

作者： Kim, Haseong Seong, Wonjae Rha, Eugene Lee, Hyewon Kim, Seong Keun Kwon, Kil Koang Park, Kwang-Hyun Lee, Dae-Hee Lee, Seung-Goo Korea Res Inst Biosci & Biotechnol KRIBB Synthet Biol & Bioengn Res Ctr Daejeon 34141 South Korea KRIBB Dis Target Struct Res Ctr Daejeon 34141 South Korea Univ Sci & Technol KRIBB Sch Biotechnol Dept Biosyst & Bioengn Daejeon 34113 South Korea

Bacteria initiate complicated signaling cascades from the detection of intracellular metabolites or exogenous substances by hundreds of transcription factors, which have been widely investigated as genetically-encoded biosensors for molecular recognition. However, the limited number of transcription factors and their broad substrate specificity result in ambiguity in small molecule identification. This study presents a new small molecule fingerprinting technique using evolutionary biosensor arrays with a machine learning technique that can capture highly specific substrate signals. Employing multiple mutant transcription factors derived from a single transcription factor has effectively circumvented the limited availability of transcription factors induced by a small molecule of our interest. This method achieved up to 95.3% true positive rate for identifying small molecules, and the high-resolution protein engineering technique improved the limit of detection 75-fold. The signal trade-offs with background noises caused by the complex cellular biochemistry of mutant transcription factors enable the biosensor arrays to be more informative in terms of statistical variance. The machine learning technology, coupled with the single transcription factor-driven evolutionary biosensor array, will open new avenues for molecular fingerprinting technologies.

关键词： Genetically-encoded biosensors Machine learning Molecular identification Protein engineering biosensor array High-throughput screening system

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Amperometric Electrochemical Microsystem for a Miniaturized Protein biosensor array

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IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS 2009年第3期3卷 160-168页

作者： Yang, Chao Huang, Yue Hassler, Brian L. Worden, R. Mark Mason, Andrew J. Marvell Semicond Inc Santa Clara CA 95054 USA Michigan State Univ E Lansing MI 48824 USA

Protein-based bioelectrochemical interfaces offer great potential for rapid detection, continuous use, and miniaturized sensor arrays. This paper introduces a microsystem platform that enables multiple bioelectrochemical interfaces to be interrogated simultaneously by an onchip amperometric readout system. A post-complementary metal-oxide semiconductor (CMOS) fabrication procedure is described that permits the formation of planar electrode arrays and self assembly of biosensor interfaces on the electrodes. The onchip, 0.5-mu m CMOS readout electronics include a compact potentiostat that supports a very broad range of input currents-6 pA to 10 mu A-to accommodate diverse biosensor interfaces. The 2.3 x 2.2-mm chip operates from a 5-V supply at 0.6 mA. A prototype electrochemical sensor platform, including an onchip potentiostat and miniaturized biosensor array, was characterized by using cyclic voltammetry. The linear relationship between the oxidization peak values and the concentrations of target analytes in the solution verifies functionality of the system and demonstrates the potential for future implementations of this platform in high sensitivity, low cost, and onchip protein-based sensor arrays.

关键词： Amperometric readout bioelectronic sensor biosensor array complementary metal-oxide semiconductor (CMOS) potentiostat electrochemical sensor

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Mass producible miniaturized flow through a device with a biosensor array

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SENSORS AND ACTUATORS B-CHEMICAL 1997年第1-3期43卷 121-125页

作者： Jobst, G Moser, I Svasek, P Varahram, M Trajanoski, Z Wach, P Kotanko, P Skrabal, F Urban, G Vienna Tech Univ Inst Allgemeine Elektrotech & Elektron A-1040 Vienna Austria Vienna Tech Univ Ludwig Boltzmann Inst Biomed Mikrotech & Hirnkrei A-1040 Vienna Austria Graz Tech Univ Dept Biophys Inst Biomed Engn A-8010 Graz Austria Krankenhaus Barmherzigen Bruder Dept Internal Med A-8020 Graz Austria

A mass producible miniaturized device for the simultaneous monitoring of different metabolites was realized by assembling of a biosensor array produced by thin film technology with a flow through cell produced by printed circuit board technology. The biosensor array comprises four working electrodes which can be individually configured. Glucose-lactate devices were made for human whole blood monitoring. Ex vivo experiments, performed with human volunteers, where the device was continuously operated in an extra corporeal undiluted heparinized blood stream for 6 h, gave close tracing to laboratory techniques by using in vitro calibration without loss in sensitivity. (C) 1997 Elsevier Science S.A.

关键词： biosensor array microsystem technology whole blood monitoring

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A fully integrated biosensor array for measurement of metabolic parameters in human blood 2

A fully integrated biosensor array for measurement of metabo...

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2nd Annual International IEEE/EMBS Conference on Microtechnologies in Medicine and Biology

作者： Gao, C Choi, JW Dutta, M Chilukuru, S Nevin, JH Lee, JY Bissell, MG Ahn, CH Univ Cincinnati Dept Elect & Comp Engn MicroSyst & BioMEMS Lab Cincinnati OH 45221 USA

ISBN: (纸本)0780374800

This paper presents a fully integrated biosensor array, which includes an oxygen sensor and a glucose sensor, for measurement of metabolic parameters in human blood. Partial oxygen concentration and glucose concentration in human blood were successfully detected in less than one minute using the developed biosensor array. Total sensor design is based on low melting point gel-based solid electrolyte that simplified fabrication process. Most parameters that are relative to sensor performance were calibrated and compared to optimize the sensor design. Such parameters include membrane material, thickness of membrane and solid electrolyte layer, area of electrodes, distance between electrodes, and the shape of electrodes. We also integrated the developed biosensor array with microfluidic systems for precise control of sample delivery.

关键词： biosensor array metabolic parameters human blood

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Electrochemical biosensor array for liver diagnosis using a silanization technique on nano-porous silicon electrode

Electrochemical biosensor array for liver diagnosis using a ...

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IEEE Nanotechnology Materials and Devices Conference

作者： Song, Min-Jung Yun, Dong-Hwa Min, Nam-Ki Hong, Suk-In Korea Univ Dept Chem & Biol Engn Seoul 136701 South Korea

ISBN: (纸本)9781424405404

Cholesterol, bilirubin and ALT/AST present in the serum are often used as biomarkers for liver diseases. For this study, we describe our biosensor array system consisting of cholesterol, bilirubin and glutamate sensors. We determined that porous silicon (PS) layers formed on each working electrode greatly increased the effective surface area. The electrodes in the sampling wells minimized a cross-interference effect to permit multiple sampling by immobilization of the enzymes using a silanization technique. The device sensitivities observed were 0.2656 mu A/mM for cholesterol, 0.15354 mA/mM for bilirubin, 0.13698 mu A/(U/l) for ALT and 0.45439 mu A/(U/l) for AST.

关键词： cholesterol sensr bilirubin sensor glutamate sensor ALT/AST activities biosensor array porous silicon electrochemical biosensor

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Measurement of meat freshness in situ with a biosensor array

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Food Control 1993年第3期4卷 149-154页

作者： Kress-Rogers, Erika Sollars, Jane E. Gibbs, Paul A. Turner, Anthony P.F. ATI Sensor Applications Ltd Ripley Surrey GU23 6LF 4 Willow Drive Sendmarsh United Kingdom Cranfield Biotechnology Ltd Newport-Pagnell Bucks MK16 9QS 20 Howard Way United Kingdom Leatherhead Food Research Association Leatherhead Surrey KT22 7RY Randalls Road United Kingdom Cranfield Biotechnology Centre Cranfield Institute of Technology Cranfield Bedford MK43 OAL United Kingdom

A prototype probe intended for the ultrarapid assessment of meat freshness is described. It provides a glucose concentration measurement at depths of 2 and 4 mm below the meat surface with the help of a biosensor array. The probe was evaluated in a 14-day trial with chill-stored pork loins carrying a slaughterhouse flora. The results indicate that the development of a knife probe for the in situ assessment of meat freshness is feasible based on a biosensor array that provides glucose depth profiling close to the meat surface. Data from other trials are taken into account in the discussion. The probe development is presented as an application of the marker approach for the rapid assessment of complex food conditions such as microbial or oxidative spoilage, ripening or the progress of fermentation. © 1993.

关键词： biosensor array Meat freshness rapid methods

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A 16x20 Electrochemical CMOS biosensor array with In-Pixel Averaging Using Polar Modulation

A 16x20 Electrochemical CMOS Biosensor Array with In-Pixel A...

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IEEE Custom Integrated Circuits Conference (CICC)

作者： Hsu, Chung-Lun Sun, Alexander Zhao, Yunting Aronoff-Spencer, Eliah Hall, Drew A. Univ Calif San Diego Dept Elect & Comp Engn La Jolla CA 92093 USA Univ Calif San Diego Sch Med La Jolla CA 92093 USA

ISBN: (纸本)9781538624838

biosensors based on electrochemical impedance spectroscopy (EIS), an ultra-sensitive, label-free sensing technique, are a promising technology for precise and rapid disease diagnosis at the point-of-care (POC). However, EIS usually requires mixers and lock-in detection to measure both the magnitude and phase of the complex impedance. To address this issue, we report a 16x20 electrochemical biosensor array with on chip sensors that implements a polar-mode measurement method that allows the readout circuitry to be mostly digital and small enough to fit within a 140x140 mu m(2) pixel. The architecture enables in-pixel digitization and accumulation, which increases the SNR by 10 dB for each 10x increase in readout time. Implemented in a 0.18 mu m process, the 3x4 mm(2) chip achieves state-of-the-art performance with an rms phase error of 0.04% at 50 kHz and was used to measure hybridization of Zika virus oligonucleotides.

关键词： point-of-care (POC) electrochemical impedance spectroscopy (EIS) biosensor array phase-to-digital converter

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