检索结果-内蒙古大学图书馆

Enumeration of circulating tumor cells and investigation of cellular responses using aptamer-immobilized AlGaN/GaN high electron mobility transistor sensor array

引用

SENSORS AND ACTUATORS B-CHEMICAL 2018年 257卷 96-104页

作者： Pulikkathodi, Anil Kumar Sarangadharan, Indu Hsu, Chen-Pin Chen, Yi-Hong Hung, Lien-Yu Lee, Geng-Yen Chyi, Jen-Inn Lee, Gwo-Bin Wang, Yu-Lin Natl Tsing Hua Univ Inst NanoEngn & MicroSyst Hsinchu 300 Taiwan Natl Tsing Hua Univ Dept Power Mech Engn Hsinchu 300 Taiwan Natl Tsing Hua Univ Inst Biomed Engn Hsinchu 300 Taiwan Natl Cent Univ Dept Elect Engn Jhongli 320 Taoyuan County Taiwan

This study reports the fabrication and characterization of electrical double layer gated AlGaN/GaN High electron mobility (HEMT) biosensor array to capture, detect and count circulating tumor cells (CTCs) of colorectal cancer (CRC). GaN HEMT chips were assembled into a sensor array on a thermo-curable polymer substrate in a simple and robust packaging process. The array had multiple aptamer immobilized sensing sites and was highly sensitive and selective with up to single cell resolution. The present method can detect cells in their native electrolyte composition, in a very short time with extremely low cost compared to the conventional circulating tumor cell assays. The effect of cellular binding in different test environments was further studied and reported. The electrical response of the sensor was discovered to be relevant to the transmembrane potential of the cell. This whole cell sensor array has the potential to be used as a point-of-care diagnostic tool in varied applications such as detection of rare cells, pathogens and studying the dynamics of cellular interactions. (c) 2017 Elsevier B.V. All rights reserved.

关键词： Whole cell sensor AlGaN/GaN HEMT biosensor array Colorectal cancer Circulating tumor cells

来源：评论

学校读者我要写书评

暂无评论

Process Integration of Ion Sensitive Field Effect Transistor Bio-Sensor array Platform

引用

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 2017年第11期17卷 8321-8325页

作者： Ha, Taemin Lee, Wooje Hong, Sang Jeen Myongji Univ Dept Elect Engn Yongin 17058 South Korea

Ion sensitive field effect transistor (ISFET) is a chemically activating electronic device for measuring pre-determined ion concentration of a solution in a way of measuring proton concentration of the solution. In this study, we present the process integration of ISFET based arrayed biosensor consists of 9 channels including reference electrode. The biosensor arrays, individual die size is 6.55 X 4.55 mm(2), are fabricated on a 6 inch silicon wafer with 15 photolithography steps. 17 process modules, including source/drain implanting, gate oxide, signal path, passivation and the forming of electrode, are integrated for the fabrication of the biosensor array platform. With the test circuit constructed for pH measure on chips, it is demonstrated that the basic functionality of the fabricated biosensor array with the sensor reading accuracy in two decimal points in the mixture of buffer solution. We convinced that the presented platform fabrication technology can lead the development of biosensor chip applications with further investigation of membrane technology for the detection of various bio and chemical related matters.

关键词： ISFET Process Integration biosensor array

来源：评论

学校读者我要写书评

暂无评论

Magnetoresistive biosensors with on-chip pulsed excitation and magnetic correlated double sampling

引用

SCIENTIFIC REPORTS 2018年第1期8卷 16493-16493页

作者： Kim, Kyunglok Hall, Drew A. Yao, Chengyang Lee, Jung-Rok Ooi, Chin C. Bechstein, Daniel J. B. Guo, Yue Wang, Shan X. Stanford Univ Dept Elect Engn Stanford CA 94305 USA Univ Calif San Diego Dept Elect & Comp Engn La Jolla CA 92093 USA Ewha Womans Univ Div Mech & Biomed Engn Seoul South Korea Stanford Univ Dept Chem Engn Stanford CA 94305 USA Stanford Univ Dept Mech Engn Stanford CA 94305 USA Stanford Univ Dept Mat Sci & Engn Stanford CA 94305 USA

Giant magnetoresistive (GMR) sensors have been shown to be among the most sensitive biosensors reported. While high-density and scalable sensor arrays are desirable for achieving multiplex detection, scalability remains challenging because of long data acquisition time using conventional readout methods. In this paper, we present a scalable magnetoresistive biosensor array with an on-chip magnetic field generator and a high-speed data acquisition method. The on-chip field generators enable magnetic correlated double sampling (MCDS) and global chopper stabilization to suppress 1/f noise and offset. A measurement with the proposed system takes only 20 ms, approximately 50x faster than conventional frequency domain analysis. A corresponding time domain temperature correction technique is also presented and shown to be able to remove temperature dependence from the measured signal without extra measurements or reference sensors. Measurements demonstrate detection of magnetic nanoparticles (MNPs) at a signal level as low as 6.92 ppm. The small form factor enables the proposed platform to be portable as well as having high sensitivity and rapid readout, desirable features for next generation diagnostic systems, especially in point-of-care (POC) settings.

来源：评论

学校读者我要写书评

暂无评论

A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic biosensor array

引用

SENSORS 2011年第10期11卷 9300-9312页

作者： Lee, Dongjin Ondrake, Janet Cui, Tianhong Univ Minnesota Dept Mech Engn Minneapolis MN 55455 USA Ohio No Univ Dept Mech Engn Ada OH 45810 USA

We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I-V measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4-12 nA/mM for channel lengths of 5-20 mu m, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.

关键词： biosensor array nanoparticle conductometric sensor microsensor array glucose sensor

来源：评论

学校读者我要写书评

暂无评论

Prediction of wastewater quality using amperometric bioelectronic tongues

引用

biosensorS & BIOELECTRONICS 2016年 75卷 375-382页

作者： Czolkos, Ilja Dock, Eva Tonning, Erik Christensen, Jakob Winther-Nielsen, Margrethe Carlsson, Charlotte Mojzikova, Renata Skladal, Petr Wollenberger, Ulla Norgaard, Lars Ruzgas, Tautgirdas Emneus, Jenny Tech Univ Denmark DTU Nanotech Dept Micro & Nanotechnol DK-2800 Lyngby Denmark AkzoNobel Ind Coatings AB S-20517 Malmo Sweden Fiberline Composites AS DK-5500 Middelfart Denmark Umetrics AB S-21134 Malmo Sweden DHI Water & Environm Dept Environm Risk Assessment DK-2970 Horsholm Denmark AnoxKaldnes AB S-22647 Lund Sweden Palacky Univ Olumouc Fac Dent & Med Dept Biol Olomouc 77515 Czech Republic Masaryk Univ Inst Biochem CS-61137 Brno Czech Republic Univ Potsdam Inst Biochem & Biol Analyt Biochem D-14476 Golm Germany Univ Copenhagen Dept Food Sci Fac Sci DK-1958 Frederiksberg Denmark Malmo Univ Fac Hlth & Soc Dept Biomed Sci S-20506 Malmo Sweden

Wastewater samples from a Swedish chemi-thermo-mechanical pulp (CTMP) mill collected at different purification stages in a wastewater treatment plant (WWTP) were analyzed with an amperometric enzyme-based biosensor array in a flow-injection system. In order to resolve the complex composition of the wastewater, the array consists of several sensing elements which yield a multidimensional response. We used principal component analysis (PCA) to decompose the array's responses, and found that wastewater with different degrees of pollution can be differentiated. With the help of partial least squares regression (PLS-R), we could link the sensor responses to the Microtox (R) toxicity parameter, as well as to global organic pollution parameters (COD, BOD, and TOC). From investigating the influences of individual sensors in the array, it was found that the best models were in most cases obtained when all sensors in the array were included in the PLS-R model. We find that fast simultaneous determination of several global environmental parameters characterizing wastewaters is possible with this kind of biosensor array, in particular because of the link between the sensor responses and the biological effect onto the ecosystem into which the wastewater would be released. In conjunction with multivariate data analysis tools, there is strong potential to reduce the total time until a result is yielded from days to a few minutes. (C) 2015 Elsevier B.V. All rights reserved.

关键词： biosensor array Electronic tongue Amperometric sensor Screen-printed electrode Multivariate data analysis Chemometrics Wastewater Toxicity Phenolic compounds

来源：评论

学校读者我要写书评

暂无评论

CMOS Amperometric Instrumentation and Packaging for biosensor array Applications

引用

IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS 2011年第5期5卷 439-448页

作者： Li, Lin Liu, Xiaowen Qureshi, Waqar A. Mason, Andrew J. Michigan State Univ Dept Elect & Comp Engn E Lansing MI 48824 USA

An integrated CMOS amperometric instrument with on-chip electrodes and packaging for biosensor arrays is presented. The mixed-signal integrated circuit supports a variety of electrochemical measurement techniques including linear sweep, constant potential, cyclic and pulse voltammetry. Implemented in 0.5 mu m CMOS, the 3 x 3 mm(2) chip dissipates 22.5 mW for a 200 kHz clock. The highly programmable chip provides a wide range of user-controlled stimulus rate and amplitude settings with a maximum scan range of 2 V and scan rates between 1 mV/sec and 400 V/sec. The amperometric readout circuit provides +/- 500 fA linear resolution and supports inputs up to +/- 47 mu A. A 2 x 2 gold electrode array was fabricated on the surface of the CMOS instrumentation chip. An all-parylene packaging scheme was developed for compatibility with liquid test environments as well as a harsh piranha electrode cleaning process. The chip was tested using cyclic voltammetry of different concentrations of potassium ferricyanide at 100 mV/s and 200 mV/s, and results were identical to measurements using commercial instruments.

关键词： biosensor array biosensor packaging CMOS amperometry CMOS potentiostat

来源：评论

学校读者我要写书评

暂无评论

A plasmonic biosensor array by block copolymer lithography

引用

JOURNAL OF MATERIALS CHEMISTRY 2010年第34期20卷 7241-7247页

作者： Shin, Dong Ok Jeong, Jong-Ryul Han, Tae Hee Koo, Chong Min Park, Hye-Jeong Lim, Yong Taik Kim, Sang Ouk Chungnam Natl Univ Grad Sch Analyt Sci & Technol Taejon 305764 South Korea Korea Adv Inst Sci & Technol Dept Mat Sci & Engn Taejon 305701 South Korea Chungnam Natl Univ Dept Mat Sci & Engn Taejon 305764 South Korea Chungnam Natl Univ Grad Sch Green Energy Technol Taejon 305764 South Korea Korea Inst Sci & Technol Polymer Hybrids Ctr Seoul South Korea Samsung Mobile Display OLED Product Dev Team Cheonan Chungcheong Nam South Korea

Highly uniform and dense, hexagonal noble metal nanoparticle arrays were achieved on large-area transparent glass substrates via scalable, parallel processing of block copolymer lithography. Exploring their localized surface plasmon resonance (LSPR) characteristics revealed that the Ag nanoparticle array displayed a UV-vis absorbance spectrum sufficiently narrow and intense for biosensing application. A highly-sensitive, label-free detection of prostate cancer specific antibody (anti-PSA) with sub-ng ml(-1) level detection limit (0.1 similar to 1 ng ml(-1)) has been accomplished with the plasmonic nanostructure. Our approach offers a valuable route to a low-cost, manufacture-scale production of plasmonic nanostructures, potentially useful for various photonic and optoelectronic devices.

关键词： .Plasmonic LSPR biosensor array Ag nanoparticle

来源：评论

学校读者我要写书评

暂无评论

Label-free electrochemical DNA biosensor array for simultaneous detection of the HIV-1 and HIV-2 oligonucleotides incorporating different hairpin-DNA probes and redox indicator

引用

biosensorS & BIOELECTRONICS 2010年第5期25卷 1088-1094页

作者： Zhang, Dongdong Peng, Yage Qi, Honglan Gao, Qiang Zhang, Chengxiao Shaanxi Normal Univ Sch Chem & Mat Sci Minist Educ Key Lab Appl Surface & Colloid Chem Xian 710062 Peoples R China

A label-free electrochemical DNA biosensor array was developed as a model system for simultaneous detection of multiplexed DNAs using microlitres of sample. A novel multi-electrode array was comprised of six gold working electrodes and a gold auxiliary electrode, which were fabricated by gold sputtering technology, and a printed Ag/AgCl reference electrode was fabricated by screen-printing technology. The DNA biosensor array for simultaneous detection of the human immunodeficiency virus (HIV) oligonucleotide sequences, HIV-1 and HIV-2, was fabricated in sequence by self-assembling each of two kinds of thiolated hairpin-DNA probes onto the surfaces of the corresponding three working electrodes, respectively. The hybridization events were monitored by square wave voltammetry using methylene blue(MB) as a hybridization redox indicator. The oxidation currents of MB accumulated on the array decreased with increasing the concentration of HIVs due to higher affinity of MB for single strand rather than double strands of DNA. Under the optimized conditions, the peak currents were linear over ranges from 20 to 100 nM for HIV-1 and HIV-2, with the same detection limits of 0.1 nM (S/N = 3), respectively. The biosensor array showed a good specificity without the obvious cross-interference. Furthermore, single-base mutation oligonucleotides and random oligonucleotides can be easily discriminated from complementary target DNAs. This work demonstrates that different hairpin-DNA probes can be used to design the label-free electrochemical biosensor array for simultaneous detection of multiplexed DNA sequences for various clinical applications. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

关键词： biosensor array DNA hybridization Electroanalysis Simultaneous detection Human immunodeficiency virus

来源：评论

学校读者我要写书评

暂无评论

Disposable micro-fluidic biosensor array for online parallelized cell adhesion kinetics analysis on quartz crystal resonators

引用

MEASUREMENT SCIENCE AND TECHNOLOGY 2010年第8期21卷 085801-085801页

作者： Cama, G. Jacobs, T. Dimaki, M. I. Svendsen, W. E. Hauptmann, P. Naumann, M. Otto VonGuericke Univ Magdegurg Inst Micro & Sensor Syst D-39106 Magdeburg Germany Otto VonGuericke Univ Magdegurg Inst Expt Internal Med D-39120 Magdeburg Germany Tech Univ Denmark Dept Micro & Nanotechnol DK-2800 Lyngby Denmark

In this contribution we present a new disposable micro-fluidic biosensor array for the online analysis of adherent Madin Darby canine kidney (MDCK-II) cells on quartz crystal resonators (QCRs). The device was conceived for the parallel cultivation of cells providing the same experimental conditions among all the sensors of the array. As well, dedicated sensor interface electronics were developed and optimized for fast spectra acquisition of all 16 QCRs with a miniaturized impedance analyzer. This allowed performing cell cultivation experiments for the observation of fast cellular reaction kinetics with focus on the comparison of the resulting sensor signals influenced by different cell distributions on the sensor surface. To prove the assumption of equal flow circulation within the symmetric micro-channel network and support the hypothesis of identical cultivation conditions for the cells living above the sensors, the influence of fabrication tolerances on the flow regime has been simulated. As well, the shear stress on the adherent cell layer due to the flowing media was characterized. Injection molding technology was chosen for the cheap mass production of disposable devices. Furthermore, the injection molding process was simulated in order to optimize the mold geometry and minimize the shrinkage and the warpage of the parts. MDCK-II cells were cultivated in the biosensor array. Parallel cultivation of cells on the gold surface of the QCRs led to first observations of the impact of the cell distribution on the sensor signals during cell cultivation. Indeed, the initial cell distribution revealed a significant influence on the changes in the measured acoustic load on the QCRs suggesting dissimilar cell migrations as well as proliferation kinetics of a non-confluent MDCK-II cell layer.

关键词： acoustic biosensors microfluidics injection molding cell adhesion biosensor array impedance spectroscopy

来源：评论

学校读者我要写书评

暂无评论

An Electrochemical biosensor array for Rapid Detection of Alanine Aminotransferase and Aspartate Aminotransferase

引用

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2009年第3期73卷 474-478页

作者： Song, Min-Jung Yun, Dong-Hwa Hong, Suk-In Korea Univ Dept Chem & Biol Engn Seoul 136701 South Korea Korea Univ Sch Elect Engn Seoul 136701 South Korea

An increment of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) in human serum indicates an abnormal symptom of the liver. Hence, an electrochemical biosensor array that uses micro electromechanical systems technology is required for rapid and integrated measurement of ALT/AST. Here we describe a biosensor array consisting of two glutamate sensors. It turned out that porous silicon layers formed on each working electrode were useful to increase the effective surface area. This biosensor array was constructed with platinum electrodes and a polydimethylsiloxane (PDMS) microchannel. Electrodes in sampling wells minimized a cross-interference effect and permitted multiple sampling by immobilization with glutamate oxidase using a silanization technique. The device sensitivities derived from semi-logarithmic plots were 0.145 mu A/(U/l) for ALT and 0.463 mu A/(U/l) for AST over a range of 1.3 U/l to 250 U/l. Hene, this ALT/AST biosensor array can be applied in diagnostic and home use.

关键词： glutamate sensor alanine aminotransferase (ALT)/aspartate aminotraseferase (AST) activities biosensor array porous silicon electrochemical biosensor

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：