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characterization of betalain-loaded liposomes and its bioactive potential in vivo after ingestion

FOOD cHEMISTRY 2023年 407卷 135180页

作者： Martinez-Rodriguez, Pedro Alejandra Guerrero-Rubio, M. Hernandez-Garcia, Samanta Henarejos-Escudero, Paula Garcia-carmona, Francisco Gandia-Herrero, Fernando Univ Murcia Dept Bioquim & Biol Mol A Unidad Docente Biol Fac Vet Reg Campus Int Excellence Campus Mare Nos Murcia Spain Univ Cambridge Dept Plant Sci Cambridge CB2 3EA England

Betalains are plant pigments characterized by showing a wide range of beneficial properties for health. Its bioactive potential has been studied for the first time after its encapsulation in liposomes and subsequent administration to the animal model caenorhabditis elegans. Phenylalanine-betaxanthin and indoline carboxylic acid-betacyanin encapsulated at concentrations of 25 and 500 mu M managed to reduce lipid accumulation and oxidative stress in the nematodes. Highly antioxidant betalains dopaxanthin and betanidin were also included in the survival analyses. The results showed that phenylalanine-betaxanthin was the most effective betalain by increasing the lifespan of c. elegans by 21.8%. In addition, the administration of encapsulated natural betanidin increased the nematodes' survival rate by up to 13.8%. The preservation of the bioactive properties of betalains manifested in this study means that the stabilization of the plant pigments through encapsulation in liposomes can be postulated as a new way for administration in pharmacological and food applications.

关键词： Antilipidemic Antioxidant Betalain c elegans In vivo Liposome

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Identification of Burkholderia cenocepacia non-coding RNAs expressed during caenorhabditis elegans infection

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APPLIED MIcROBIOLOGY AND BIOTEcHNOLOGY 2023年第11期107卷 3653-3671页

作者： Pita, Tiago Feliciano, Joana R. Leitao, Jorge H. Univ Lisbon iBB Inst Bioengn & Biosci Dept Bioengn P-1049001 Lisbon Portugal Univ Lisbon i4HB Inst Hlth & Bioecon Inst Super Tecn Associate Lab Inst Super Tecn P-1049001 Lisbon Portugal

Small non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Despite the identification of hundreds of bacterial sRNAs, their roles on bacterial physiology and virulence remain largely unknown, as is the case of bacteria of the Burkholderia cepacia complex (Bcc). Bcc is a group of opportunistic pathogens with relatively large genomes that can cause lethal lung infections amongst cystic fibrosis (cF) patients. To characterise sRNAs expressed by Bcc bacteria when infecting a host, the nematode caenorhabditis elegans was used as an infection model by the epidemic cF strain B. cenocepacia J2315. A total of 108 new and 31 previously described sRNAs with a predicted Rho independent terminator were identified, most of them located on chromosome 1. RIT11b, a sRNA downregulated under c. elegans infection conditions, was shown to directly affect B. cenocepacia virulence, biofilm formation, and swimming motility. RIT11b overexpression reduced the expression of the direct targets dusA and pyrc, involved in biofilm formation, epithelial cell adherence, and chronic infections in other organisms. The in vitro direct interaction of RIT11b with the dusA and pyrc messengers was demonstrated by electrophoretic mobility shift assays. To the best of our knowledge this is the first report on the functional characterization of a sRNA directly involved in B. cenocepacia virulence.

关键词： Burkholderia cenocepacia c elegans infection model RNA-seq Non-coding RNAs

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Activation of FGF signal in germline mediates transgenerational toxicity of polystyrene nanoparticles at predicted environmental concentrations in caenorhabditis elegans

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JOURNAL OF HAZARDOUS MATERIALS 2023年第1期451卷 131174页

作者： Hua, Xin cao, chang Zhang, Le Wang, Dayong Southeast Univ Med Sch Key Lab Environm Med Engn Minist Educ Nanjing 210009 Peoples R China Shenzhen Ruipuxun Acad Stem Cell & Regenerat Med Shenzhen 518122 Peoples R China

Nanoplastics in the environment could cause the ecological and health risks. Recently, the transgenerational toxicity of nanoplastic has been observed in different animal models. In this study, using caenorhabditis elegans as an animal model, we aimed to examine the role of alteration in germline fibroblast growth factor (FGF) signal in mediating the transgenerational toxicity of polystyrene nanoparticle (PS-NP). Exposure to 1-100 mu g/L PS-NP (20 nm) induced transgenerational increase in expressions of germline FGF ligand/EGL-17 and LRP-1 governing FGF secretion. Germline RNAi of egl-17 and lrp-1 resulted in resistance to transgenerational PS-NP toxicity, indicating the requirement of FGF ligand activation and secretion in formation of transgenerational PS-NP toxicity. Germline overexpression of EGL-17 increased expression of FGF receptor/EGL-15 in the offspring, and RNAi of egl-15 at F1 generation (F1-G) inhibited transgenerational toxicity of PS-NP exposed animals overexpressing germline EGL-17. EGL-15 functions in both the intestine and the neurons to control transgenerational PS-NP toxicity. Intestinal EGL-15 acted upstream of DAF-16 and BAR-1, and neuronal EGL-15 functioned upstream of MPK-1 to control PS-NP toxicity. Our results suggested the important role of activation in germline FGF signal in mediating the induction of transgenerational toxicity in organisms exposed to nanoplastics in the range of mu g/L.

关键词： Transgenerational toxicity Nanoplastic FGF c elegans

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A wideband accurate compact 6-bit phase shifter in 0.15-μm GaAs technology

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MIcROWAVE AND OPTIcAL TEcHNOLOGY LETTERS 2023年第2期65卷 534-540页

作者： Zeng, Jialong Xie, chunshuang Zhang, Desheng Feng, Jie Tan, cheng Yu, Zhongjun Chinese Acad Sci Aerosp Informat Res Inst Beijing Peoples R China Univ Chinese Acad Sci Sch Elect Elect & Commun Engn Beijing Peoples R China

A Ku wideband accurate compact 6-bit digital phase shifter is designed in this letter using three different switched phase shift structures: the switched L/c structure, T-type structure, and high-pass/low-pass network. To improve the bandwidth and the gain flatness, an improved switched L/c structure is used and the systematic cascade sequence of the phase shifter is optimized. The improved switched L/c structure solves the problem that conventional L/c networks are hypersensitive to the coupling capacitance at high-frequency input. As a result, the improved L/c structure can achieve wideband and better s-parameters. The proposed 6-bit switched phase shifter is implemented in 0.15-mu m GaAs pHEMT process. The measured root-mean-square (rms) phase error is less than 2.8 degrees at 12-18 GHz, and the average insertion loss (IL) is 6.5-6.9 dB. The chip size of the proposed 6-bit digital phase shifter is 3.1 x 1 mm(2), including all RF and dc pads, which is very suitable for radar systems.

关键词： digital phase shifter high-pass low-pass network root-mean-square (rms) switched L c T-type structure

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Relationship analyses on environmental factors-ablation performance based on Zrc-Tac system: Oxygen partial pressure and gas flow scouring

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JOURNAL OF THE EUROPEAN cERAMIc SOcIETY 2023年第6期43卷 2331-2344页

作者： Hu, Dou Zhang, Yutai Dong, Zhijie Wang, Hanhui Fu, Qiangang Northwestern Polytech Univ State Key Lab Solidificat Proc Shaanxi Key Lab Fiber Reinforced Light Weight Com Xian 710072 Shaanxi Peoples R China

For better understanding on effects of ablation environmental factors (oxygen partial pressure P(O2) and gas flow scouring), multiple experiments and multiscale simulations based on thermally sprayed Zrc-Tac system were conducted. High P(O2) was found helpful to accelerate Ta2O5/ZrO2 mutual diffusion by variable P(O2) oxidation at 1500 degrees c (5-50 kPa). Effects of gas flow scouring was evaluated by flow filed simulation coupling laser and oxyacetylene ablations, from which its promotion effect on Ta2O5/ZrO2 diffusion efficiency was found. The increased Ta2O5/ZrO2 diffusion efficiency will accelerate the structure instability of Ta doped c-ZrO2 skeletons due to Zr-O bond length extension around Ta atoms.

关键词： c c composites Tac Ablation First principal calculation Zrc

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Recent progress in the gasification reaction behavior of coal char under unconventional combustion modes

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APPLIED THERMAL ENGINEERING 2023年 220卷

作者： Zhang, Zewu Zhao, Zhenghong Zhang, Liqi Huazhong Univ Sci & Technol Sch Energy & Power Engn State Key Lab Coal Combust Wuhan 430074 Hubei Peoples R China

compared with conventional air combustion, the combustion mode and medium of unconventional combustion technologies (oxy-fuel combustion, moderate or intense low oxygen dilution (MILD) combustion, MILD-oxy combustion, pressurized oxy-fuel combustion (POc), and chemical looping combustion (cLc)) exhibit signifi-cant changes, and the gasification behavior of coal char also changes, which can influence the flame temperature, heat transfer, char combustion, NOx emissions, and even particulate matter (PM) formation. This study sys-temically reviews the current progress in coal char gasification reaction behavior under different unconventional combustion modes. First, the combustion principle and char gasification behavior during unconventional com-bustion modes of pulverized coal are summarized. Next, the effects of the operating conditions (such as tem-perature, bulk diffusivity, and species concentration, etc.) on the gasification behavior are discussed. The transformation between the combustion and gasification regimes during pulverized coal combustion can be realized by adjusting the operating parameters. In addition, the influencing mechanisms of the gasification re-action on pulverized coal combustion, including flame temperature, heat transfer, char burnout, NOx emissions and PM formation, are clarified. Especially, NOx emissions can be further reduced by enhancing char gasification with three aspects: the dilution oxygen effect, homogeneous reduction effect, and heterogeneous reduction ef-fect. Besides, the current challenges of these unconventional combustion technologies are summarized, and their application scenarios are expected to achieve cO2 mitigation targets. Finally, this work concludes it necessary to explore the internal association between enhanced gasification and reduced NOx emissions, which is expected to provide valuable guidance for c/N synergetic reduced emissions in the wide applications of carbon-based solid fuels.

关键词： Pulverized coal Oxy-fuel combustion Heat transfer char gasification NOx emissions c N synergetic reduced emissions

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氯化镧影响大鼠海马ATP含量、cOX IV及cyt c表达的研究

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毒理学杂志 2020年第1期34卷 1-5页

作者：袁慧孙文昌洪云婷张彦强王剑波蔡原杨敬华中国医科大学公共卫生学院卫生毒理学教研室辽宁沈阳110122

目的探究氯化镧对大鼠海马三磷酸腺苷(Adenosine triph osphate,ATP)含量、细胞色素c氧化酶4(cytochrome c oxidase IV,cOX IV)活性、cOX IV蛋白、线粒体和胞浆中细胞色素c(cytochrome c,cyt c)蛋白表达水平的影响,探究镧损害学习记忆... 详细信息

目的探究氯化镧对大鼠海马三磷酸腺苷(Adenosine triph osphate,ATP)含量、细胞色素c氧化酶4(cytochrome c oxidase IV,cOX IV)活性、cOX IV蛋白、线粒体和胞浆中细胞色素c(cytochrome c,cyt c)蛋白表达水平的影响,探究镧损害学习记忆能力的机制。方法32只成年雌性Wistar大鼠随机分为对照组、低、中和高剂量染镧组,在孕哺期各组分别饮用蒸馏水、0.125%、0.25%和0.5%的Lacl3溶液。仔鼠通过吸吮母乳染镧,哺乳期结束后进行跳台实验,取子代大鼠海马进行ATP含量和cOX IV活性测定,采用Western blot法检测子代大鼠海马组织中cOX IV蛋白、线粒体及胞浆内cyt c蛋白水平。结果与对照组相比,中和高剂量染镧组子代大鼠在跳台实验中学习记忆能力降低、差异具有统计学意义(P<0.05),海马组织中ATP含量和cOX IV活性降低,cOX IV蛋白表达水平降低,差异具有统计学意义(P<0.05)。与对照组相比,中和高剂量染镧组子代大鼠海马线粒体内cyt c蛋白水平降低且差异具有统计学意义(P<0.05),而中和高剂量染镧组胞浆内cyt c蛋白水平升高且差异具有统计学意义(P<0.05)。结论在该试验条件下,镧损害大鼠学习记忆能力的机制可能与海马ATP含量、cOX IV活性、cOX IV蛋白水平下降及线粒体内cyt c蛋白释放到胞浆中有关。

关键词：氯化镧海马 ATP cOX IV cyt c

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Dissecting Physical and Biochemical Effects in Nanotopographical Regulation of cell Behavior

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AcS NANO 2023年第3期17卷 2124-2133页

作者： Wang, Kai Man, Kun Liu, Jiafeng Meckes, Brian Yang, Yong Univ North Texas Dept Biomed Engn Denton TX 76207 USA Schoen Adams Res Inst Spaulding Discovery Ctr Musculoskeletal Recovery Charlestown MA 02129 USA Harvard Med Sch Dept Phys Med & Rehabil Boston MA 02115 USA

Regulating cell behavior using nanotopography has been widely implemented. To facilitate cell adhesion, physical nanotopography is usually coated with adhesive proteins such as fibronectin (FN). However, the confounding effects of physical and biochemical cues of nanotopography hinder the understanding of nanotopography in regulating cell behavior, which ultimately limits the biomedical applications of nanotopography. To delineate the roles of the physical and biochemical cues in cell regulation, we fabricate substrates that have either the same physical nanotopography but different biochemical (FN) nanopatterns or identical FN nanopatterns but different physical nanotopographies. We then examine the influences of physical and biochemical cues of nanotopography on spreading, nuclear deformation, mechanotransduction, and function of human mesenchymal stem cells (hMScs). Our results reveal that physical topographies, especially nanogratings, dominantly control cell spreading, YAP localization, proliferation, and differentiation of hMScs. However, biochemical FN nanopatterns affect hMSc elongation, YAP intracellular localization, and lamin a/c (LAMAc) expression. Furthermore, we find that physical nanogratings induce nanoscale curvature of nuclei at the basal side, which attenuates the osteogenic differentiation of hMScs. collectively, our study highlights the dominant effect of physical nanotopography in regulating stem cell functions, while suggesting that fine-tuning of cell behavior can be achieved through altering the presentation of biochemical cues on substrate surfaces.

关键词： nanotopography adhesive proteins contact printing stem cells nuclear curvature lamin A c

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Nontargeted metabolomic evidence for antagonism between tetracycline and its resistance bacteria underlying their obesogenic effects on caenorhabditis elegans

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ScIENcE OF THE TOTAL ENVIRONMENT 2023年第Part1期859卷 160223页

作者： Li, Zhuo Wu, Di Yu, Zhenyang cui, changzheng Yin, Daqiang Tongji Univ Coll Environm Sci & Engn State Key Lab Pollut Control & Resource Reuse Key Lab Yangtze River Water EnvironmMinist Educ Shanghai 200092 Peoples R China Jiaxing Tongji Inst Environm Jiaxing 314051 Zhejiang Peoples R China Shanghai Inst Pollut Control & Ecol Secur Shanghai 200092 Peoples R China Shanghai Univ Tradit Chinese Med Shuguang Hosp Shanghai 201203 Peoples R China East China Univ Sci & Technol Sch Resources & Environm Engn State Environm Protect Key Lab Environm Risk Asses Shanghai 200237 Peoples R China

Environmental antibiotics raise serious health concerns due to their contribution to the obesity prevalence. Moreover, antibiotics promote antibiotic-resistance bacteria (ARB) which represent another emerging pollutant. However, the interaction between antibiotic and ARB in the obesogenic effects remained unexplored. In the present study, the obesogenic effects of tetracycline antibiotic (TcH) and ARB containing tetA were studied on c. elegans, and E. coli OP50 (OP50) was referred as a normal bacterial food. Results showed that TcH stimulated nematode triglyceride contents, while ARB alone had no significant influences. The combination of TcH and ARB showed less obesogenic effects than TcH alone, showing antagonism. Biochemical assays showed that the combination of TcH and ARB showed similar effects to ARB alone, and had less increases in lipid metabolism enzymes or metabolites than those of TcH or ARB alone, supporting the antagonism. In the nontargeted metabolomic analysis, TcH with ARB showed less significantly changed metabolites (ScMs) in the nematodes than TcH or ARB alone, partially explaining the antagonism. The metabolomic results also pointed out the significant involvement of amino acids, the carboxylic acids and derivatives, and also the benzene and substituted derivatives in the obesogenic effects of TcH and ARB. The findings of the present study provided a direct support for interaction between antibiotics and ARB underlying their health risks.

关键词： Obesogenic effect Antibiotic Antibiotic -resistance bacteria Metabolomic analysis c elegans

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Influence of a rich sulphur atmosphere on the phase development and kinetic behaviour of differentially processed RNT650?- TiAl alloys

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JOURNAL OF MATERIALS RESEARcH AND TEcHNOLOGY-JMR&T 2023年 23卷 1753-1760页

作者： Dudziak, T. Rzad, E. Morgiel, J. Kirchner, A. Polczyk, T. Moskal, G. Kloeden, B. Weissgaerber, T. Tobola, D. Wojcicki, M. Krakow Inst Technol Lukasiewicz Res Network 73 Zakopianska Str PL-30418 Krakow Poland Polish Acad Sci Inst Met & Mat Sci 25 Reymonta Str PL-30059 Krakow Poland Inst Dresden Fraunhofer Inst Fertigungstech & Angew Mat Forsch Winterbergstr 28 D-01277 Dresden Germany Silesian Tech Univ Fac Mat Engn Dept Adv Mat & Technol 8 Krasinskiego Str PL-40019 Katowice Poland

The influence of sulphur atmosphere on conventionally cast (cM) and on electron beam melted (EBM) g -TiAl RNT650 alloy exposed to a 1% (vol.) H2S -air atmosphere at 650 degrees c for 500 h was investigated. The material produced by cM showed a lower mass gain than the EBM material. XRD and TEM/EDS analyses indicated the formation of rutile-TiO2/(a-Al2-O3 + rutile-TiO2)/amorphous-Al2O3 sequence of phases. In EBM a coarse crystalline layer rich in aluminium, oxygen and a small amount of sulphur was found. The higher content of sulphur was in the scale bottom intermixed with amorphous alumina.(c) 2023 Published by Elsevier B.V. This is an open access article under the cc BY-Nc-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： A intermetallics Titanium B XRD TEM c oxidation Sulphidation

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