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Bioelectrochemistry for miRNA detection

cURRENT OPINION IN ELEcTROcHEMISTRY 2017年第1期5卷 183-192页

作者： ciui, Bianca Jambrec, Daliborka Sandulescu, Robert cristea, cecilia Iuliu Hatieganu Univ Med & Pharm Dept Analyt Chem 4 Louis Pasteur St Cluj Napoca 400349 Romania Ruhr Univ Bochum AnalyticalChem CES Univ Str 150 D-44780 Bochum Germany Univ Calif San Diego Dept Nanoengn La Jolla CA 92093 USA

Anomalous expression of miRNAs has been linked with cell cycles such as cell proliferation, stress response, differentiation, cell apoptosis and inflammation, which present a manifestation of multitude types of disorders and malignancies. For this reason, miRNAs became a class of biomarkers that is in the research focus in recent years. Their detection in an early stage of diseases represents a difficult challenge for analytical chemists who need to design not only specific but also highly sensitive tools for their detection. The progress achieved in the bio-electrochemical detection of miRNAs in the last 2 years is highlighted and commented, focusing on the applicability of developed strategies for point-of-care sensing.

关键词： LOD limit of detection LR linear range cNT carbon nanotube MWcNT multiwalled carbon nanotube PAMAM polyamidoamine MB methylene blue DPV differential pulse voltammetry MNPs magnetic nanoparticles SPEs screen-printed electrodes HT hybridization time chitin-MBs chitin-modified magnetic beads HQ hydroquinone SWV squre wave voltammetry cV cyclic voltammetry MSA mercaptosuccinicacid TcEP tris(2-carboxyethyl) phosphine hydrochloride AuNPs gold nanoparticles TDNs self-assembled tetrahedral DNA nanostructures GO graphene oxide HRP horseradish peroxidase TMB-3,3′,5,5′ tetramethylbenzidine α-NP α naphthyl phosphate ST streptavidin ALP alkaline phosphatase HQ hydroquinone Fc ferrocene RGO reduced graphene oxide MMB magnetic microbeads HcR hybridization chain reaction cyt c cytochrome c AOx alcohol oxidase RcA rolling-circle amplification AgNcs silver nanoclusters QDs quantum dots GQDs graphene quantum dots MM mismatch Nc non-complementary

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竹叶青蛇伤患者c反应蛋白和凝血功能变化及其临床意义

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中华卫生应急电子杂志 2016年第4期2卷 -页

作者：姚为学李志文陈粤明陈翠珍湛江中心人民医院 524037

目的分析竹叶青蛇伤患者 c 反应蛋白（cRP）和凝血功能变化及其临床意义。方法以2011年5月至2013年5月广东湛江中心人民医院急诊科收治的竹叶青蛇咬伤患者138例为研究对象（男性85例，女性53例；年龄14～70岁）患者按照被竹叶青蛇咬伤... 详细信息

目的分析竹叶青蛇伤患者 c 反应蛋白（cRP）和凝血功能变化及其临床意义。方法以2011年5月至2013年5月广东湛江中心人民医院急诊科收治的竹叶青蛇咬伤患者138例为研究对象（男性85例，女性53例；年龄14～70岁）患者按照被竹叶青蛇咬伤后的就诊时间分为5组（2 h 内组、3～6 h 组、7～12 h 组、13～24 h 组合25～48 h 组），比较各组间症状、体征、凝血功能及cRP 水平的差异。结果竹叶青蛇咬伤2 h 内的患者有疼痛、局部肿胀等症状，但凝血功能及 cRP 与正常对照组差异无统计学意义，咬伤12 h 后与2 h 内组相比，血小板技术降低、活化部分凝血活酶时间（APTT）增高且凝血功能及 cRP 均增高（P ＜0．05）。结论竹叶青蛇毒不仅可引起凝血功能障碍，同时可引起局部的快速炎症反应，导致肢体肿胀及激烈疼痛等，应重视抗炎治疗。

关键词：竹叶青蛇炎症 c 反应蛋白凝血功能

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Mechanical Ventilation: State of the Art

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MAYO cLINIc PROcEEDINGS 2017年第9期92卷 1382-1400页

作者： Pham, Tai Brochard, Laurent J. Slutsky, Arthur S. Univ Toronto Interdept Div Crit Care Toronto ON Canada St Michaels Hosp Keenan Res Ctr Biomed Sci Li Ka Shing Knowledge Inst Toronto ON Canada

Mechanical ventilation is the most used short-term life support technique worldwide and is applied daily for a diverse spectrum of indications, from scheduled surgical procedures to acute organ failure. This state-of-the-art review provides an update on the basic physiology of respiratory mechanics, the working principles, and the main ventilatory settings, as well as the potential complications of mechanical ventilation. Specific ventilatory approaches in particular situations such as acute respiratory distress syndrome and chronic obstructive pulmonary disease are detailed along with protective ventilation in patients with normal lungs. We also highlight recent data on patient-ventilator dyssynchrony, humidified high-flow oxygen through nasal cannula, extracorporeal life support, and the weaning phase. Finally, we discuss the future of mechanical ventilation, addressing avenues for improvement. (c) 2017 Mayo Foundation for Medical Education and Research.

关键词： ARDS acute respiratory distress syndrome cOPD chronic obstructive pulmonary disease c RS compliance of the respiratory system E RS elastance of the respiratory system FIO 2 fraction of inspired oxygen IcU intensive care unit MV mechanical ventilation NIV noninvasive ventilation ΔP pressure change PBW predicted body weight PEEP positive end-expiratory pressure P 0 initial alveolar pressure Pplat plateau pressure PSV pressure support ventilation RcT randomized controlled trial SBT spontaneous breathing trial SpO 2 pulse oximetry oxygen saturation VILI ventilator-induced lung injury Vt tidal volume

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前清蛋白、心肌酶、c 反应蛋白检测在重症手足口病合并心肌损伤患儿诊治中的意义

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中国基层医药 2016年第22期23卷 3500-3503页

作者：敖小艳浙江省长兴县人民医院儿科 313100

目的：探讨前清蛋白（PA）、心肌酶、c 反应蛋白（cRP）检测在重症手足口病（HFMD）合并心肌损伤患儿诊治中的应用价值。方法选取183例手足口病患儿作为观察组，其中重症患儿54例，普通患儿129例，另选取100例同期健康体检儿童作为对照... 详细信息

目的：探讨前清蛋白（PA）、心肌酶、c 反应蛋白（cRP）检测在重症手足口病（HFMD）合并心肌损伤患儿诊治中的应用价值。方法选取183例手足口病患儿作为观察组，其中重症患儿54例，普通患儿129例，另选取100例同期健康体检儿童作为对照组，检测所有研究对象血清 PA、α-丁酸脱氮酶（α-HBDH），肌酸激酶（cK），肌酸激酶同工酶（cK-MB）、cRP 的水平并进行比较。结果观察组血清 PA 水平为（194．37±42．86）mg／L，对照组为（267．15±52．04）mg／L，观察组血清 PA 水平明显低于对照组（t ＝5．942， P ＜0．05）；观察组血清α-HBDH、cK、cK-MB 及 cRP 分别为（261．58±61．40）U ／L、（124．80±67．51）U ／L、（38．79±16．26）U ／L 和（18．06±5．27）mg／L，对照组血清α-HBDH、cK、cK-MB 及 cRP 分别为（112．93±28．56）U ／L、（103．94±60．86）U ／L、（15．17±9．34）U ／L 和（4．82±0．95）mg／L，观察组α-HBDH、cK、cK-MB及 cRP 均明显高于对照组（t ＝8．356、5．087、9．446、10．755，均 P ＜0．05）。重症组血清 PA 水平为（144．92±40．36）mg／L，普通组为（230．89±46．27）mg／L，重症组血清 PA 水平明显低于普通组（t ＝7．035，P ＜0．05）；重症组血清α-HBDH、cK、cK-MB 及 cRP 分别为（301．58±71．40）U ／L、（145．31±67．84）U ／L、（43．66±18．95）U ／L 和（21．86±6．55）mg／L，普通组血清α-HBDH、cK、cK-MB 及 cRP 分别为（162．93±28．56）U ／L、（116．05±61．37）U ／L、（24．75±12．04）U ／L 和（7．25±3．82）mg／L，重症组患儿血清α-HBDH、cK、cK-MB 及cRP 均明显高于普通组（t ＝9．440、6．204、11．038、13．118，均 P ＜0．05）。重症 HFMD 合并心肌损伤患儿血清PA 水平为（131．02±36．54）mg／L，重症组为（149．73±42．81）mg／L，重症 HFMD 合并心肌损伤组血清 PA 水平明显低于重症组（t ＝7．801，P ＜0．05）；重症 HFMD 合并心肌损伤患儿血清α-HBDH、cK、cK-MB 及 cRP 分别为（327．84±74．95）U ／L、（152．92±68．75）U ／L、（45．17±20．68）U ／L 和（23．07±7．46）mg／L，重症组血清α-HBDH、cK、cK-MB 及 cRP 分别为（286．30±70．16）U ／L、（134．80±66．31）U ／L、（41．08±17．23）U ／L 和（20．95±6．20）mg／L，重症 HFMD 合并心肌损伤患儿血清α-HBDH、cK、cK-MB 及 cRP 均明显高于重症HFMD 组（t ＝4．394、5．938、4．077、4．165，均 P ＜0．05）。结论前清蛋白、心肌酶、cRP 检测利于手足口病患儿病情严重程度的判断，且利于患儿心肌损伤的早期诊断。

关键词：手足口病心肌损伤前清蛋白心肌酶 c 反应蛋白质

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cysc、Hcy、HbA1c、hs-cRP在糖尿病早期肾损伤中的应用价值

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中国继续医学教育 2016年第14期8卷 32-33页

作者：云绍丽董彩霞河南省临颍县人民医院检验科河南临颍462600

目的分析cys c、Hcy、Hb A1c、hs-cRP在糖尿病早期肾损伤中的应用价值。方法将我院2012年4月－2014年9月收治的62例糖尿病早期肾损伤患者作为研究组;将同期62例健康体检者作为对照组,检测两组Hb A1c、hs-cRP、cys c、Hcy等指标水平。... 详细信息

目的分析cys c、Hcy、Hb A1c、hs-cRP在糖尿病早期肾损伤中的应用价值。方法将我院2012年4月－2014年9月收治的62例糖尿病早期肾损伤患者作为研究组;将同期62例健康体检者作为对照组,检测两组Hb A1c、hs-cRP、cys c、Hcy等指标水平。统计两组研究对象Hb A1c、hs-cRP、cys c、Hcy水平,比较研究组相关指标联合检测阳性率及单独检测阳性率。结果研究组Hb A1c、hs-cRP、cys c、Hcy等指标水平均高于对照组,两组差异显著（P〈0.05）;研究组相关指标单独检测阳性率显著低于联合检测阳性率,差异显著（P〈0.05）。结论 Hb A1c、hs-cRP、cys c、Hcy均可作为糖尿病早期肾损伤的检测指标,且四项指标联合检测可有效提高其诊断的阳性率及准确率,在糖尿病早期肾损伤患者的早期诊断与治疗中具有重要意义。

关键词： cys c Hcy HbA1c hs-cRP 糖尿病早期肾损伤

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Tensile and compression Behaviour of Boron carbide Reinforced 6061Al MMc’s processed through conventional Melt Stirring

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Materials Today: Proceedings 2018年第8期5卷 16141-16145页

作者： V. Ashok Kumar M.P. Anil G.L. Rajesh Vijaykumar Hiremath V. Auradi R & D Centre Department of Mechanical Engineering Siddaganga Institute of Technology Tumakuru Karnataka India

Metal matrix composites have large application in various fields like aerospace and automobile applications due to higher strength to weight ratio owing to their excellent combination properties. considering this fact, the present work is taken up with objective of evaluating tensile and compressive behavior of 6061Al-B 4 c composite at room temperature. The composite is prepared via melt stirring method with 9wt% of boron carbide particulates of size 8 microns at processing temperature of 750°c. Microstructural studies of prepared composites were carried out using SEM analysis. Hardness measurements of the composite were carried out using micro-Vickers hardness tester with a load of 250gm and dwell time of 30sec. Tensile and compressive behaviors were studied using universal testing machine at room temperature condition. Microstructural studies revealed fairly uniform distribution of B 4 c particles in 6061Al matrix. Al-B 4 c composite has exhibited better tensile and compressive strength in comparison with matrix alone, which increases its durability and load carrying capacity. Therefore, 6061Al-B 4 c system could be used as replacement of steel and other ferrous materials in structural applications.

关键词： 6061Al B 4 c MMc’s Tensile Strength compressive Strength

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Genetic biomarkers for hepatocellular cancer risk in a caucasian population

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World Journal of Gastroenterology 2017年第36期23卷 6674-6684页

作者： Elena De Mattia Erika cecchin Jerry Polesel Alessia Bignucolo Rossana Roncato Francesco Lupo Marina crovatto Angela Buonadonna claudio Tiribelli Giuseppe Toffoli Clinical and Experimental Phar-macology “Centro di Riferimento Oncologico”-National Cancer Institute Unit of Cancer Epidemiology “Centro di Riferimento Oncologico”-National Cancer Institute General Surgery 2U and Liver Transplantation Center A.O.U.Cittàdella Salute e della Scienza di TorinoUniversity of Torino Cytogenetics and Molecular Biology Unit Santa Maria degli Angeli Hospital Pordenone Medical Oncology Unit “Centro di Riferimento Oncologico”-National Cancer Institute Fondazione Italiana Fegato AREA Science Park

AIM To uncover novel genetic markers that could contribute to predicting hepatocellular carcinoma(Hcc)susceptibility in caucasians. METHODS The present retrospective case-control study compared genotype frequencies between a cohort of Hcc cases and two,independent,Hcc-free,age/sex-matched control *** Hcc cohort comprised 192 homogeneous patients that had undergone orthotopic liver *** first control group comprised167 patients that were matched to the Hcc cohort for the percentage of hepatitis B(HBV)and/or hepatitis c(HcV)infections.A second control group included192 virus-free,healthy individuals that were used to evaluate the generalizability of the identified predictive *** cases and controls were *** three study populations were characterized with a panel of 31 markers derived from 21 genes that encoded key proteins involved in hepatocarcinogenesis-related *** study end-point was to assess the association between genetic variants and Hcc onset. RESULTS Five genetic markers were identified as risk factors for Hcc in high-risk patients infected with HBV/*** to a dominant model,reduced Hcc risk was associated with three polymorphisms:ERcc1rs3212986(OR=0.46,95%cI:0.30-0.71,P=0.0005),GST-P1 rs1138272(OR=0.41,95%cI:0.21-0.81,P=0.0097),and cYP17A1 rs743572(OR=0.50,95%cI:0.31-0.79,P=0.0032).conversely,according to a recessive model,increased Hcc risk was associated with two polymorphisms:XRcc3 rs1799794(OR=3.70,95%cI:1.02-13.39,P=0.0461)and ABcB1 rs1128503(OR=2.06,95%cI:1.18-3.61,P=0.0111).These associations remained significant in a subgroup analysis,where patients were stratified according to viral status(HBV-or HcV-positive serology).Two variants exhibited a serology-specific effect:ABcB1 rs1128503(OR=4.18,95%cI:1.55-11.29,P=0.0048)showed an effect in the HBV-positive subgroup;and ERcc1 rs3212986(OR=0.33,95%cI:0.18-0.60,P=0.0003)showed an effect in the HcV-positive *** the five markers identified,E

关键词： ERcc1 XRcc3 GST-P1 cYP17A1 MDR-1 Polymorphisms Hepatocellular carcinoma risk Early diagnosis Antiviral therapy Hepatitis B viral/hepatitis c viral

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Pegylated interferon α/ribavirin therapy enhances bone mineral density in children with chronic genotype 4 HcV infection

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World Journal of Pediatrics 2017年第4期13卷 346-352页

作者： Ahmed Megahed Nanees Salem Abeer Fathy Tarik Barakat Mona Abd El Latif Alsayed Suzy Abd El Mabood Khaled R Zalata Ahmed F Abdalla Pediatric Gastroenterology and Hepatology Unit Mansoura University Children's HospitalFaculty of MedicineMansoura UniversityEgypt Pediatric Endocrinology Unit Mansoura University Children's HospitalFaculty of MedicineMansoura UniversityEgypt Pediatric Hematology&Oncology Unit Mansoura University Children's HospitalFaculty of MedicineMansoura UniversityEgypt Pathology Department Faculty of MedicineMansoura UniversityEgypt

Background:The impact of chronic hepatitis c (cHc) on bone mineral density (BMD) has been well studied in adults with a relative paucity of data in children,especially concerning effect of treatment with pegylated interferon (PEG-IFN) plus ribavirin (RV).In the current work,we assessed prospectively changes in BMD in children with cHc before,during,and after ***:Forty-six consecutive children with noncirrhotic genotype 4 cHc were subjected to dual-energy X-ray absorptiometry at baseline,24 weeks,48 weeks of therapy and 24 weeks after ***,bone mineral content (BMc),and Z score of lumbar spine (L2-L4) were *** pubertal stage,viral load,liver function tests,serum calcium,phosphorus,alkaline phosphatase,parathyroid hormone,and liver histopathology were assessed in all included ***:Thirty (65.2%) patients had normal BMD,10 (21.7%) were at risk for low BMD,and 6 (13.1%) had low BMD for chronological *** with low BMD were significantly older (P=0.001),with higher frequency of delayed puberty than other groups (P=0.002).Baseline densitometric parameters (BMD & BMc) were significantly positively correlated with patients' age,weight,height,body mass index and hemoglobin level;while they were insignificantly correlated with basal viral load,histopathology activity index and fibrosis *** parameters improved significantly on PEG-IFN plus RV treatment,this improvement was found to be sustainable 24 weeks after ***:Low BMD is detectable in a proportion of cHc *** therapy leads to a sustainable increase in BMD.

关键词： bone mineral density chronic hepatitis c pegylated interferon ribavirin

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Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (McE) during adipocyte differentiation

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JOURNAL OF cELLULAR PHYSIOLOGY 2018年第5期233卷 4205-4215页

作者： chan, chien-Yi Yu, Ping chang, Feng-Tzu chen, Zih-Hua Lee, Ming-Fen Huang, chun-Yin China Med Univ Dept Nutr 91 Hsueh Shih Rd Taichung 40402 Taiwan Chang Jung Christian Univ Dept Nutr & Hlth Sci Tainan Taiwan Asia Univ Dept Hlth & Nutr Biotechnol Taichung Taiwan

Transcription factor HMG box-containing protein 1 (HBP1) has been found to be up-regulated in rat adipose tissue and differentiated preadipocyte;however, how HBP1 is involved in adipocyte formation remains unclear. In the present study, we demonstrated that under a standard differentiation protocol HBP1 expression fluctuates with down-regulation in the mitotic clonal expansion (McE) stage followed by up-regulation in the terminal differentiation stage in both 3T3-L1 and MEF cell models. Also, HBP1 knockdown accelerated cell cycle progression in the McE stage, but it impaired final adipogenesis. To gain further insight into the role of HBP1 in the McE stage, we found that the HBP1 expression pattern is reciprocal to that of c/EBP, and ectopic expression of HBP1suppresses c/EBP expression. These data indicate that HBP1 functions as a negative regulator of McE. In contrast, when HBP1 expression was gradually elevated along with a concomitant induction of c/EBP at the end of the McE, HBP1 knockdown leads to a significant reduction of c/EBP expression, suggesting that HBP1-mediated c/EBP expression may be needed for the termination of the cell cycle at the end of McE for terminal differentiation. All told, our findings show that HBP1 is a key transcription factor in the already complicated regulatory cascade during adipocyte differentiation.

关键词： adipocyte differentiation c EBP c EBP HBP1 mitotic clonal expansion

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长寿命、高倍率超级电容器电极材料的研究——三明治型MoO_3/c插层复合物的原位合成

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常熟理工学院学报 2016年第2期30卷 120-122页

作者：季红梅杨刚常熟理工学院化学与材料工程学院江苏常熟215500

利用典型的二维材料α-MoO_3为前驱物,设计并合成了一种特殊的α-MoO_3层和石墨烯插层复合材料.三明治型的MoO_3/c杂化电极材料具有宽的离子扩散通道,低的电荷迁移电阻和稳定的结构,因此具有优异的储能特性.在1 A·g^(-1)的电流密度... 详细信息

利用典型的二维材料α-MoO_3为前驱物,设计并合成了一种特殊的α-MoO_3层和石墨烯插层复合材料.三明治型的MoO_3/c杂化电极材料具有宽的离子扩散通道,低的电荷迁移电阻和稳定的结构,因此具有优异的储能特性.在1 A·g^(-1)的电流密度下,MoO_3/c复合物的比容量为331 F·g^(-1);10 A·g^(-1)时,比容量保持率达到71%.除此,该材料还具有良好的循环稳定性,在1 000~10 000圈的循环过程中比容量基本无衰减.优异的倍率性能使该电极材料具有高功率密度(12.0 k W·kg^(-1))和能量密度(41.2 Wh·kg^(-1)).

关键词：二维材料 Mo O_3 c 插层超级电容器

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