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Lamin A/c negatively regulated by miR-124-3p modulates apoptosis of vascular smooth muscle cells during cyclic stretch application in rats

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AcTA PHYSIOLOGIcA 2020年第3期228卷 e13374-e13374页

作者： Bao, Han Li, Hai-Peng Shi, Qian Huang, Kai chen, Xiao-Hu chen, Yuan-Xiu Han, Yue Xiao, Qian Yao, Qing-Ping Qi, Ying-Xin Shanghai Jiao Tong Univ Inst Mechanobiol & Med Engn Sch Life Sci & Biotechnol Shanghai Peoples R China Beihang Univ Key Lab Biomech & Mechanobiol Minist Educ Sch Biol Sci & Med Engn Beijing Peoples R China Beihang Univ Beijing Adv Innovat Ctr Biomed Engn Beijing Peoples R China

Aim Apoptosis of vascular smooth muscle cells (VSMcs) influenced by abnormal cyclic stretch is crucial for vascular remodelling during hypertension. Lamin A/c, a nuclear envelope protein, is mechano-responsive, but the role of lamin A/c in VSMc apoptosis is still unclear. Methods FX-5000T Strain Unit provided cyclic stretch (cS) in vitro. AnnexinV/PI and cleaved caspase 3 ELISA detected apoptosis. qPcR was used to investigate the expression of miR-124-3p and a luciferase reporter assay was used to evaluate the ability of miR-124-3p binding to the Lmna 3'UTR. Protein changes of lamin A/c and relevant molecules were detected using western blot. Ingenuity Pathway Analysis and Protein/DNA array detected the potential transcription factors. Renal hypertensive rats verified these changes. Results High cyclic stretch (15%-cS) induced VSMc apoptosis and repressed lamin A/c expressions compared with normal (5%-cS) control. Downregulation of lamin A/c enhanced VSMc apoptosis. In addition, 15%-cS had no significant effect on mRNA expression of Lmna, and lamin A/c degradation was not induced by autophagy. 15%-cS elevated miR-124-3p bound to the 3'UTR of Lmna and negatively regulated protein expression of lamin A/c. Similar changes occurred in renal hypertensive rats compared with sham controls. Lamin A/c repression affected activity of TP53, cREB1, MYc, STAT1/5/6 and JUN, which may in turn affect apoptosis. conclusion Our data suggested that the decreased expression of lamin A/c upon abnormal cyclic stretch and hypertension may induce VSMc apoptosis. These mechano-responsive factors play important roles in VSMc apoptosis and might be novel therapeutic targets for vascular remodelling in hypertension.

关键词： apoptosis cyclic stretch lamin A c mechanobiology vascular smooth muscle cells

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Loss of myeloid-specific lamin A/c drives lung metastasis through Gfi-1 and c/EBPε-mediated granulocytic differentiation

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MOLEcULAR cARcINOGENESIS 2020年第7期59卷 679-690页

作者： Ishii, Hiroki Park, Woo-Yong So, Jaeyoung Kuhn, Skyler Koparde, Vishal N. Pang, Yanli Greten, Tim F. Hollander, M. christine Yang, Li NCI Lab Canc Biol & Genet Ctr Canc Res NIH Bethesda MD 20892 USA NCI CCR Collaborat Bioinformat Resource Ctr Canc Res NIH Bethesda MD 20892 USA NCI Adv Biomed Computat Sci Frederick Natl Lab Canc Res NIH Frederick MD 21701 USA NCI Thorac & Gastrointestinal Malignancies Branch Ctr Canc Res NIH Bethesda MD 20892 USA

The immune-suppressive tumor microenvironment promotes metastatic spread and outgrowth. One of the major contributors is tumor-associated myeloid cells. However, the molecular mechanisms regulating their differentiation and function are not well understood. Here we report lamin A/c, a nuclear lamina protein associated with chromatin remodeling, was one of the critical regulators in cellular reprogramming of tumor-associated myeloid cells. Using myeloid-specific lamin A/c knockout mice and triple-negative breast cancer (TNBc) mouse models, we discovered that the loss of lamin A/c drives cD11b(+)Ly6G(+) granulocytic lineage differentiation, alters the production of inflammatory chemokines, decreases host antitumor immunity, and increases metastasis. The underlying mechanisms involve an increased H3K4me3 leading to the upregulation of transcription factors (TFs) Gfi-1 and c/EBP epsilon. Decreased lamin A/c and increased Gfi-1 and c/EBP epsilon were also found in the granulocytic subset in the peripheral blood of human cancer patients. Our data provide a mechanistic understanding of myeloid lineage differentiation and function in the immune-suppressive microenvironment in TNBc metastasis.

关键词： granulocytic differentiation immunosuppression lamin A c metastasis myeloid cells

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Nico2O4/c core-Shell Nanoneedles on Ni Foam for All-Solid-State Asymmetric Supercapacitors

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cHEMISTRYSELEcT 2020年第19期5卷 5501-5506页

作者： Li, Yaoyin Wang, Qiyuan Shao, Jian Li, Kang Zhao, Weiwei Harbin Inst Technol Flexible Printed Elect Technol Ctr Shenzhen 518085 Peoples R China Harbin Inst Technol Sch Mat Sci & Engn Shenzhen 518085 Peoples R China Harbin Inst Technol State Key Lab Adv Welding & Joining Shenzhen 518085 Peoples R China

Herein, Nico2O4/c core-shell nanoneedles grown on Ni foam are prepared by a simple route and applied in supercapacitors. Electrochemical results show that the area specific capacitance of Nico2O4/c reaches 2057 mF/cm(2) at 1 mA/cm(2), which is about 4 times than that of the pristine Nico2O4. The electrode exhibits excellent rate capability (92 % of capacitance retention at 10 mA/cm(2)) and remarkable cycling stability (81 % of capacitance retention after 10000 cycles). The as-fabricated all-solid-state asymmetric supercapacitor (Nico2O4/c//Ac(active carbon)) achieves an energy density of 2 mW h cm(-3) at a power density of 37 mW cm(-3) and remains 1.6 mW h cm(-3) at a power density of 120 mW cm(-3). Moreover, the ASc device can retain 87 % capacitance after 4000 cycles. The result reveals that it is a feasible method to achieve excellent performance in energy storage devices by the dipping method.

关键词： core-shell Energy conversion Nanostructures Nico2O4 c

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New c,O-Glycosylflavones from the Genus Silene

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cHEMISTRY OF NATURAL cOMPOUNDS 2020年第6期56卷 1026-1034页

作者： Olennikov, D. N. Kashchenko, N. I. Russian Acad Sci Siberian Branch Inst Gen & Expt Biol 6 Sakhyanovoi St Ulan Ude 670047 Russia

chromatographic separation of extracts from the aerial parts of three Silene species (caryophyllaceae) isolated 26 flavonoids including the four new c,O-glycosylflavones acacetin-6-c-(2 ''-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (sileneside D, 1) from S. jeniseensis Willd., apigenin-6-c-(2 ''-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside-8-c-alpha-L-arabinopyranoside (sileneside E, 2) and genkwanin-6-c-(2 ''-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside-8-c-alpha-L-arabinopyranoside (sileneside F, 3) from S. italica (L.) Pers., and apigenin-6-c-(2 ''-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside-7-O-(6 ''''-O-feruloyl)-beta-D-glucopyranoside (sileneside G, 4) from S. dioica (L.) clairv. The structures of 1-4 were studied using UV, IR, and NMR spectroscopy and mass spectrometry.

关键词： Silene jeniseensis Silene italica Silene dioica caryophyllaceae c O-glycosylflavones sileneside

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Response of Dendrobium officinale Kimura et Migo, a Prized Medicinal Plant, to continuous UV-B Irradiation at Different c/N Ratios

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JOURNAL OF PLANT GROWTH REGULATION 2020年第1期39卷 358-369页

作者： cui, Dandan Mo, Yuncai Zeng, Lingjie Feng, Kai Feng, Xiaoyun Huang, Jialing He, Mengling Zhang, Xiaoyuan Teng, Xifeng Guangdong Pharmaceut Univ Sch Chinese Med Guangzhou 510006 Peoples R China Key Lab State Adm Tradit Chinese Med Prod & Dev C Guangzhou 510006 Peoples R China South China Univ Technol Shaoguan Inst High Tech Ind Shaoguan 512028 Peoples R China

The response of Dendrobium officinale Kimura et Migo (D. officinale) to continuous UV-B irradiation at different carbon to nitrogen ratios (c/N ratios) was investigated. Seedlings grown for 60 days were incubated under aseptic conditions with UV-B irradiation (15.6 mu W cm(-2)) at different c/N ratios: control group (cK;c/N 30 without UV-B), UV-B + cK (c/N 30 with UV-B irradiation, similarly hereafter), UV-B + c/N 120, UV-B + c/N 60, UV-B + c/N 15, UV-B + c/N 10, UV-B + c/N 7.5. Growth parameters (the defoliation rate and the sprout number), photosynthetic pigments (carotenoids, chlorophyll a and chlorophyll b), total polysaccharides, total alkaloids, and activities of antioxidant enzymes were determined following 4, 8, 12, and 16 days of continuous UV-B exposure. Results indicated that UV-B irradiation increased the defoliation rate and the content of carotenoids, total polysaccharides and total alkaloids, as well as the activities of antioxidant enzymes. conversely, UV-B irradiation reduced the sprout number and chlorophyll content in D. officinale. compared with UV-B + cK, lower c/N ratio treatments (UV-B + c/N 15, UV-B + c/N 10 and UV-B + c/N 7.5) enhanced the defoliation rate and sprout number, but decreased antioxidant enzyme activities and total polysaccharide content during the whole period, and reduced total alkaloid content after 4 days of UV-B exposure. Following initial UV-B irradiation, lower c/N ratios increased the contents of carotenoid and chlorophyll b, while after 8 days, a reversal in carotenoid content was observed, and after 12 days, a reversal in chlorophyll b content was observed. Optimizing the c/N ratio (c/N 60) resulted in lower defoliation rate, higher photosynthetic pigments and total polysaccharides, and increased activities of antioxidant enzymes, whereas no significant change in sprout number and total alkaloid content was recorded under long-term UV-B irradiation. Furthermore, the UV-B + c/N 120 treatment negatively affected D. offic

关键词： Dendrobium officinale Kimura et Migo UV-B irradiation c N ratio Photosynthetic pigments Antioxidant enzymes Main active compounds

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维生素c防治2型糖尿病研究进展

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食品工业科技 2021年第15期42卷 372-376页

作者：丁丽娜王志斌王瑶宁杰张宪党丁文宇山东第一医科大学附属内分泌与代谢病医院(山东省内分泌与代谢病研究所) 山东济南250062 山东第一医科大学(山东省医学科学院) 山东济南250062

2型糖尿病(T2DM)是由胰岛素缺乏和胰岛素抵抗引起的复杂代谢紊乱性疾病。维生素c(Vc)是人体必需的营养素,是众所周知的抗氧化剂,可保护细胞免受氧化应激的伤害。T2DM患者的氧化应激状态对并发症的发生发展起重要作用,适量补充Vc可降低T... 详细信息

2型糖尿病(T2DM)是由胰岛素缺乏和胰岛素抵抗引起的复杂代谢紊乱性疾病。维生素c(Vc)是人体必需的营养素,是众所周知的抗氧化剂,可保护细胞免受氧化应激的伤害。T2DM患者的氧化应激状态对并发症的发生发展起重要作用,适量补充Vc可降低T2DM的发病风险。Vc与T2DM及其并发症密切相关,T2DM患者血浆Vc水平普遍偏低,且血浆Vc水平与T2DM的患病风险呈负相关;补充Vc可以改善胰岛素抵抗、改善炎症状态,同时可延缓糖尿病肾病的发生发展和心血管的发生风险,但在糖尿病牙周疾病方面并没有明显的改善作用。本文对Vc与T2DM发生发展中的作用予以综述,旨在为T2DM的防治提供一定的参考。

关键词：维生素 c 2 型糖尿病氧化应激自由基抗氧化剂

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Mechano-gated channels in c. elegans

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JOURNAL OF NEUROGENETIcS 2020年第3-4期34卷 363-368页

作者： Al-Sheikh, Umar Kang, Lijun Zhejiang Univ Sch Med Affiliated Hosp 1 Dept Neurobiol Hangzhou Zhejiang Peoples R China Zhejiang Univ Sch Med Affiliated Hosp 1 Dept Neurosurg Hangzhou Zhejiang Peoples R China Zhejiang Univ Sch Brain Sci & Brain Med MOE Frontier Sci Ctr Brain Res & Brain Machine In NHC & CAMS Key Lab Med Neurobiol Hangzhou Zhejiang Peoples R China

Mechanosensation such as touch, hearing and proprioception, is functionally regulated by mechano-gated ion channels through the process of transduction. Mechano-gated channels are a subtype of gated ion channels engaged in converting mechanical stimuli to chemical or electrical signals thereby modulating sensation. To date, a few families of mechano-gated channels (DEG/ENac, TRPN, K2P, TMc and Piezo) have been identified in eukaryotes. Using a tractable genetic model organism caenorhabditis elegans, the molecular mechanism of mechanosensation have been the focus of much research to comprehend the process of mechanotransduction. comprising of almost all metazoans classes of ion channels, transporters and receptors, c. elegans is a powerful genetic model to explore mechanosensitive behaviors such as touch sensation and proprioception. The nematode relies primarily on its sensory abilities to survive in its natural environment. Genetic screening, calcium imaging and electrophysiological analysis have established that ENac proteins and TRPN channel (TRP-4 protein) can characterize mechano-gated channels in c. elegans. A recent study reported that TMcs are likely the pore-forming subunit of a mechano-gated channel in c. elegans. Nevertheless, it still remains unclear whether Piezo as well as other candidate proteins can form mechano-gated channels in c. elegans.

关键词： Mechano-gated ion channel mechanotransduction mechanosensation c elegans

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Magnetic-Field-Regulated TiO2 {100} Facets: A Strategy for c-c coupling in cO2 Photocatalytic conversion

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cHEM 2020年第9期6卷 2335-2346页

作者： Jiang, Meng-Pei Huang, Ke-Ke Liu, Jing-Hai Wang, Dan Wang, Ying Wang, Xia Li, Zhi-Da Wang, Xi-Yang Geng, Zhi-Bin Hou, Xiang-Yan Feng, Shou-Hua Jilin Univ Coll Chem Int Joint Res Lab Nano Micro Architecture Chem State Key Lab Inorgan Synth & Preparat ChemNMAC Changchun Peoples R China Inner Mongolia Univ Nationalities Coll Chem & Chem Engn Nano Innovat Inst NII Inner Mongolia Key Lab Carbon Nanomat Tong Liao Peoples R China Chinese Acad Sci Inst Proc Engn State Key Lab Biochem Engn Beijing Peoples R China Chinese Acad Sci Changchun Inst Appl Chem Changchun Peoples R China Northeast Petr Univ Coll Chem & Chem Engn Prov Key Lab Oil & Gas Chem Technol Daqing Peoples R China

Solar-driven cO2 conversion is an attractive option for producing usable fuels and chemicals. However, traditionally synthesized TiO2 materials suffer from the low activity of cO2 conversion into multi-carbon products. In this study, for the first time, strong magnetic fields were introduced into the synthesis of TiO2. By regulating the splitting ratio of high-angle and low-angle quantum orbitals, we developed a new type of TiO2{100} facets containing more active low-coordinate Ti atoms. In-situ Fourier transform infrared spectroscopy (FTIR) and DFT calculations both revealed that the interfacial charge redistribution and lattice structure of such TiO2{100} are beneficial to the coupling of adsorbed cO*. This enables highly efficient conversion of cO2 into c2H5OH with a yield rate of 6.16 mmol g(-1) h(-1), which is 22-fold higher than that of pristine TiO2. This strategy provides a new platform for desirable photocatalyst synthesis and furthers our understanding of the relationship between atomic orbital control and cO2 conversion.

关键词： cO 2 conversion TiO 2 facets orbital splitting active low-coordinate Ti atoms c-c coupling c 2 H 5 OH SDG7: Affordable and clean energy

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Social and sexual behaviors in c. elegans: the first fifty years

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JOURNAL OF NEUROGENETIcS 2020年第3-4期34卷 389-394页

作者： Portman, Douglas S. Univ Rochester Del Monte Inst Neurosci Dept Biomed Genet 601 Elmwood AveBox 633 Rochester NY 14642 USA Univ Rochester Del Monte Inst Neurosci Dept Neurosci 601 Elmwood AveBox 633 Rochester NY 14642 USA Univ Rochester Del Monte Inst Neurosci Dept Biol 601 Elmwood AveBox 633 Rochester NY 14642 USA

For the first 25 years after the landmark 1974 paper that launched the field, most c. elegans biologists were content to think of their subjects as solitary creatures. c. elegans presented no shortage of fascinating biological problems, but some of the features that led Brenner to settle on this species-in particular, its free-living, self-fertilizing lifestyle-also seemed to reduce its potential for interesting social behavior. That perspective soon changed, with the last two decades bringing remarkable progress in identifying and understanding the complex interactions between worms. The growing appreciation that c. elegans behavior can only be meaningfully understood in the context of its ecology and evolution ensures that the coming years will see similarly exciting progress.

关键词： c elegans social behavior sexual behavior pheromones

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Microbial Shifts and Shorter Time to Bowel Resection Surgery Associated with c. difficile in Pediatric crohn's Disease

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INFLAMMATORY BOWEL DISEASES 2020年第8期26卷 1212-1221页

作者： Hellmann, Jennifer Andersen, Heidi Fei, Lin Linn, Aaron Bezold, Ramona Lake, Kathleen Jackson, Kimberly Meyer, Danielle Dirksing, Kelsie Bonkowski, Erin Ollberding, Nicholas J. Haslam, David B. Denson, Lee Cincinnati Childrens Hosp Med Ctr Div Gastroenterol Hepatol & Nutr Cincinnati OH 45229 USA Cincinnati Childrens Hosp Med Ctr Div Infect Dis Cincinnati OH 45229 USA Cincinnati Childrens Hosp Med Ctr Div Biostat & Epidemiol Dept Pediat Cincinnati OH 45229 USA Univ Cincinnati Coll Med Cincinnati OH USA

Background clostridioides difficile infection and colonization are common in pediatric crohn's disease (cD). Our aims were to test the relationship between c. difficile positivity and bowel resection surgery and to characterize microbial shifts associated with c. difficile carriage and surgery. Methods A retrospective single-center study of 75 pediatric cD patients tested for association between c. difficile carriage and bowel resection surgery. A prospective single-center study of 70 cD patients utilized c. difficile testing and shotgun metagenomic sequencing of fecal samples to define microbiota variation stratified by c. difficile carriage or history of surgery. Results The rate of bowel resection surgery increased from 21% in those without c. difficile to 67% in those with (P = 0.003). From a Kaplan-Meier survival model, the hazard ratio for time to first surgery was 4.4 (95% cI, 1.2-16.2;P = 0.00) in patients with positive c. difficile testing in the first year after diagnosis. Multivariable logistic regression analysis confirmed this association (odds ratio 16.2;95% cI, 2.2-120;P = 0.006). Larger differences in microbial abundance and metabolic pathways were observed in patients with prior surgery than in those with c. difficile carriage. Depletion of Alistipes and Ruminococcus species and reduction in methionine biosynthesis were noted in patients with both c. difficile carriage and past surgery. conclusions A positive c. difficile test during the first year after diagnosis is associated with decreased time to first bowel resection surgery in pediatric crohn's disease. Depletion of beneficial commensals and methionine biosynthesis in patients with c. difficile carriage may contribute to increased risk for surgery. In the first year after diagnosis, c. difficile is associated with shorter time to first surgery in pediatric crohn's disease. Reduction in beneficial commensals and methionine biosynthesis in those with c. difficile may contribute to this increased

关键词： c difficile infection pediatric inflammatory bowel disease bowel resection surgery shotgun metagenomic sequencing fecal microbiome

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