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The MIR34B/c genomic region contains multiple potential regulators of multiciliogenesis

FEBS LETTERS 2023年第12期597卷 1623-1637页

作者： cavard, Amelie Redman, Elisa Mercey, Olivier Abelanet, Sophie Plaisant, Magali Arguel, Marie-Jeanne Magnone, Virginie Garcia, Sandra Ruiz Rios, Geraldine Deprez, Marie Lebrigand, Kevin Ponzio, Gilles caballero, Ignacio Barbry, Pascal Zaragosi, Laure-Emmanuelle Marcet, Brice Univ Cote Azur CNRS IPMC Sophia Antipolis France Ecole Normale Super Inst Biol Paris France Univ Tours ISP INRA Nouzilly France IPMC CNRS UMR 7275 660 Route Lucioles F-06560 Valbonne France

The MIR449 genomic locus encompasses several regulators of multiciliated cell (Mcc) formation (multiciliogenesis). The miR-449 homologs miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN, and HOATZ, located in the MIR34B/c locus using single-cell RNA-seq and super-resolution microscopy from human, mouse, or pig multiciliogenesis models. BTG4, LAYN, and HOATZ transcripts were expressed in both precursors and mature Mccs. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/c locus may gather potential actors of multiciliogenesis.

关键词： BTG4 HOATZ LAYN miR-34b c motile cilia multiciliated cells

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TLR3 activation with poly I:c exacerbates escalated alcohol consumption in dependent male c57BL/6J mice

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AMERIcAN JOURNAL OF DRUG AND ALcOHOL ABUSE 2023年第3期49卷 290-301页

作者： Gano, Anny Lebonville, christina L. Becker, Howard c. Med Univ South Carolina Charleston Alcohol Res Ctr Dept Psychiat & Behav Sci Charleston SC 29425 USA Med Univ South Carolina Dept Neurosci Charleston SC 29425 USA RHJ Dept Vet Affairs Med Ctr Charleston SC USA

Background: Activation of TLR3 receptors, which are sensitive to viral infection, has emerged as a possible mechanism that increases alcohol intake in rodents. Objectives: These studies examined whether a history of ethanol dependence exacerbated the increase in drinking driven by the TLR3 agonist poly I:c. Methods: Male c57BL/6J mice (>10 per group) were given access to ethanol (20% v/v) 2 hours a day following a history of home cage drinking or after having been rendered ethanol-dependent using a chronic intermittent ethanol (cIE) vapor model. After testing multiple doses, a 5 mg/kg repeated poly I:c challenge was used to probe the effects of repeated immune challenge, alone or in conjunction with repeated cycles of cIE, on voluntary drinking. An ethanol (12% v/v) operant self-administration model was used to test the effects of poly I:c on stress-induced reinstatement of ethanol seeking and consumption. Results: Poly I:c in naive animals resulted in transient, modest increases in ethanol intake in the home cage and in self-administration (p < 0.05). However, poly I:c challenge resulted in sensitized stress-induced ethanol consumption and evoked a strong and persistent escalation of drinking in mice with a history of dependence (p < 0.05 for both). conclusion: Activation of viral immune defense may affect ethanol consumption in dependence and sensitivity to future stressors. As patients who suffer from alcohol use disorder are at a heightened risk for viral infection, this interaction could generate risk factors for exacerbating behaviors associated with Alcohol Use Disorders via an immune mechanism.

关键词： TLR3 poly I c cIE voluntary drinking self-administration stress TMT

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Statics and Kinetics of Sorption of La(III), ce(III), U(VI) Ions by a Nanostructured composite Al2O3||c

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RADIOcHEMISTRY 2023年第1期65卷 68-80页

作者： Polyakov, E. V. Volkov, I. V. Krasil'nikov, V. N. Ioshin, A. A. Russian Acad Sci Inst Solid State Chem Ural Branch Ekaterinburg 620990 Russia

The sorption properties of the new Al2O3||c composite were studied. A feature of its macroscopic morphology and structure is the amorphous state of alumina generated in an inert atmosphere in chemical contact with the graphene-like carbon surface. This feature is shown to determine the high chemical activity of the alumina-based composite (Ac) in an aqueous medium, which is comparable with the activity of alpha-Al2O3 or gibbsite Al(OH)(3). The sorption properties of the obtained composite towards La(III), ce(III), U(VI) ions are described within the surface complexation model. The agreement between the values of the first hydrolysis constant of La(III), ce(III), and U(VI) cations calculated from the sorption experiment and published data proves the adequacy of the chosen sorption model. It follows from simulation that the sorption activity of the composite is caused by a higher constant (K-1a) of the acid dissociation of -OH2surf+ groups (pK(1a) = 3.9) of the amorphous Ac surface and the partial distribution coefficients of individual hydroxo complexes of the studied cations. Sorption of ions by the composite in a solution of humic acids (HA) is completely determined by the parallel reaction of competitive sorption of protonated HA anions by the amorphous Ac surface in the HA concentration range of 10-200 mg/L. Al2O3||c is an effective sorbent for trace amounts of La(III), ce(III), U(VI), their chemical analogs, and humic acid in natural and working solutions with a low salt background.

关键词： Al2O3 c composite Al(III) oxides La(III) ce(III) U(VI) ions sorption statics kinetics humic acids

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Evolutionary implications of c2 photosynthesis: how complex biochemical trade-offs may limit c4 evolution

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JOURNAL OF EXPERIMENTAL BOTANY 2023年第3期74卷 707-722页

作者： Walsh, catherine A. Braeutigam, Andrea Roberts, Michael R. Lundgren, Marjorie R. Univ Lancaster Lancaster Environm Ctr Lancaster LA1 4YQ England Bielefeld Univ Fac Biol Univ Str 27 D-33615 Bielefeld Germany

The c-2 carbon-concentrating mechanism increases net cO2 assimilation by shuttling photorespiratory cO2 in the form of glycine from mesophyll to bundle sheath cells, where cO2 concentrates and can be re-assimilated. This glycine shuttle also releases NH3 and serine into the bundle sheath, and modelling studies suggest that this influx of NH3 may cause a nitrogen imbalance between the two cell types that selects for the c-4 carbon-concentrating mechanism. Here we provide an alternative hypothesis outlining mechanisms by which bundle sheath NH3 and serine play vital roles to not only influence the status of c-2 plants along the c-3 to c-4 evolutionary trajectory, but to also convey stress tolerance to these unique plants. Our hypothesis explains how an optimized bundle sheath nitrogen hub interacts with sulfur and carbon metabolism to mitigate the effects of high photorespiratory conditions. While c-2 photosynthesis is typically cited for its intermediary role in c-4 photosynthesis evolution, our alternative hypothesis provides a mechanism to explain why some c-2 lineages have not made this transition. We propose that stress resilience, coupled with open flux tricarboxylic acid and photorespiration pathways, conveys an advantage to c-2 plants in fluctuating environments. An alternative theory for bundle sheath ammonia tolerance in c(2)species suggests that c(2)to c(4)evolutionary transitions may depend on bundle sheath nitrogen hub optimization.

关键词： c-2 photosynthesis c-3-c-4 intermediates c-4 evolution carbon-concentrating mechanism c N balance GABA glycine shuttle nitrogen sink photorespiration serine tricarboxylic acid pathway

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Tetra-ARMS-PcR assay development for genotyping of AGT rs699 T/c polymorphism, its comparison with PcR-RFLP and application in a case-control association study of cardiovascular disease patients

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NUcLEOSIDES NUcLEOTIDES & NUcLEIc AcIDS 2023年第8期42卷 603-618页

作者： Hussain, Misbah Khan, Haq Nawaz Abbas, Shahid Ali, Ansar Aslam, Muhammad Naeem Awan, Fazli Rabbi Natl Inst Biotechnol & Genet Engn NIBGE Diabet & Cardiometab Disorders Lab Human Mol Genet & Metab Disorders Grp Hlth Biotechnol Faisalabad Pakistan Univ Sargodha Dept Biotechnol Sargodha Pakistan NIBGE Coll Pakistan Inst Engn & Appl Sci NIBGE C PIEAS Islamabad Pakistan Aga Khan Univ Dept Biol & Biomed Sci Karachi Pakistan Faisalabad Inst Cardiol Faisalabad Pakistan Faisalabad Med Univ Allied Hosp Faisalabad Pakistan Natl Inst Biotechnol & Genet Engn NIBGE Diabet & Cardiometab disorders lab Hlth Biotechnol Div Human Mol Genet & Metab Disorders Grp Jhang Rd Faisalabad Pakistan

Genetic variations in the AGT gene play a significant role in controlling the plasma concentration of angiotensinogen (precursor protein of bioactive octapeptide angiotensin II) and the efficacy of antihypertensive drugs. In the current study, Tetra-Amplification Refractory Mutation System-Polymerase chain Reaction (T-ARMS-PcR) was developed for genotyping of AGT rs699 T/c polymorphism and validated through Sanger DNA sequencing. Its efficiency was also tested using 474 human DNA samples [control, n = 181;cardiovascular disease (cVD) patients, n = 293]. Results showed that T-ARMS-PcR is superior to the commonly used PcR-Restriction Fragment Length Polymorphism (PcR-RFLP). Statistical analysis revealed that the AGT rs699 cc genotype is more prevalent in the cVD patient group (37% vs. 28%) and AGT rs699 c allele and cc genotype increased the risk of cVD by 1.4 and 1.9 fold, respectively. In summary, T-ARMS-PcR is the most suitable approach for quick and efficient genotyping of AGT rs699 T/c polymorphism in a large population in resource-limited countries, Furthermore, AGT rs699 T/c polymorphism is associated with the risk of cVD in the Punjabi Pakistani population.

关键词： Amplification refractory mutation system-PcR (ARMS-PcR) restriction fragment length polymorphism (RFLP) hypertension angiotensinogen rs699 T c cardiovascular disease

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Adult-restricted gene knock-down reveals candidates that affect locomotive healthspan in c. elegans

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BIOGERONTOLOGY 2023年第2期24卷 225-233页

作者： Jushaj, Areta churgin, Matthew de la Torre, Miguel Kieswetter, Amanda Driesschaert, Brecht Dhondt, Ineke Braeckman, Bart P. Fang-Yen, christopher Temmerman, Liesbet Katholieke Univ Leuven Dept Biol Anim Physiol & Neurobiol Leuven Belgium Univ Penn Dept Bioengn Philadelphia PA USA Univ Ghent Dept Biol Lab Aging Physiol & Mol Evolut Ghent Belgium

Understanding how we can age healthily is a challenge at the heart of biogerontological interest. Whereas myriad genes are known to affect the lifespan of model organisms, effects of such interventions on healthspan-the period of life where an animal is considered healthy, rather than merely alive-are less clear. To understand relationships between life- and healthspan, in recent years several platforms were developed with the purpose of assessing both readouts simultaneously. We here relied on one such platform, the WorMotel, to study effects of adulthood-restricted knock-down of 130 caenorhabditis elegans genes on the locomotive health of the animals along their lifespans. We found that knock-down of six genes affected healthspan while lifespan remained unchanged. For two of these, F26A3.4 and chn-1, knock-down resulted in an improvement of healthspan. In follow-up experiments we showed that knockdown of F26A3.4 indeed improves locomotive health and muscle structure at old age.

关键词： c elegans Aging Healthspan 4 Locomotion WorMotel

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Au-doped nanostructured TiO2/c material derived from MIL-125 as a highly sensitive electrochemical sensor for ferulic acid

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JOURNAL OF cOORDINATION cHEMISTRY 2023年第7-8期76卷 1007-1019页

作者： Li, Gaihua Liu, Shuang Liu, Yanjun Pang, Xiaoyu Li, Miao Gong, Yachang Wu, Yao Guo, Xinjie Tangshan Normal Univ Dept Chem Key Lab Green Specialty Chem Tangshan Tangshan Hebei Peoples R China Tangshan Normal Univ Dept Chem Key Lab Green Specialty Chem Tangshan Tangshan 063000 Hebei Peoples R China

An Au-doped nanostructured TiO2/c material was synthesized by a facile approach via calcination of Ti-based metal-organic-framework (MIL-125) with Au-doped from HAucl4 and was used to fabricate a modified glassy carbon electrode (GcE) for detection of ferulic acid (FA). The composition and morphology of TiO2/c/Au were characterized by powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The redox properties were investigated by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, the reported sensor not only exhibited high selectivity, but also showed a wide linear range over FA concentrations from 0 to 20.0 mu M with a low detection limit of 1.6 x 10(-8 )mol/L, attributed to advantageously high electron transfer efficiency.

关键词： TiO2 c Au nanocomposite ferulic acid electrochemical sensor

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Effects of temperature experienced during embryonic development on biomass and c and N composition at hatching in Palaemon serratus (Pennant, 1777)

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AcTA ZOOLOGIcA 2023年第4期104卷 677-685页

作者： Baudet, Jean-Baptiste Xuereb, Benoit Danger, Michael Felten, Vincent Duflot, Aurelie Maniez, Emeline Le Foll, Frank coulaud, Romain Le Havre Normandie Univ ULHN FR CNRS SCALE 3730 UMR INERIS Environm Stresses & Biomonitoring Aquat Le Havre France Univ Lorraine UMR CNRS 73602 LIEC F-73602 Metz France Le Havre Normandie Univ ULHN FR CNRS SCALE 3730 UMR INERIS Environm Stresses & Biomonitoring Aquat 25 Rue Philippe Bon F-76600 Le Havre France

In decapod crustaceans, the conditions experienced during embryonic development trigger phenotypic plasticity of the larvae at hatching. The objective of this study was to test the effects of temperature during embryonic development of Palaemon serratus on the phenotypic plasticity of hatching larvae. We incubated egg-bearing females from eggs laying to hatching at four temperatures (10, 15, 18 and 20 degrees c). Weight, carbon and nitrogen contents were measured on newly laid eggs and on freshly hatched larvae. The duration of embryonic development was negatively correlated with incubation temperature. At 20 degrees c, all females abandoned their eggs during development. Incubation temperature had no effect on the weight and the percentage of N of the larvae at hatching, while it did affect their percentage of c and their c/N ratio. Embryos incubated at 10 degrees c seemed to produce larvae with fewer lipid reserves than those incubated at 15 and 18 degrees c. They probably overconsumed their lipid reserves to compensate for the metabolic losses due to the low temperature. These results provide information on the link between maternal investment per egg and larval development in P. serratus.

关键词： c N decapod eggs larvae phenotypic plasticity

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Aliasing Limits on Translating c to Safe Rust

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PROcEEDINGS OF THE AcM ON PROGRAMMING LANGUAGES-PAcMPL 2023年第OOPSLA期7卷 551-579页

作者： Emre, Mehmet Boyland, Peter Parekh, Aesha Schroeder, Ryan Dewey, Kyle Hardekopf, Ben Univ San Francisco San Francisco CA 94117 USA Univ Calif Santa Barbara Santa Barbara CA 93106 USA Calif State Univ Northridge Northridge CA 91330 USA

The Rust language was created to provide safe low-level systems programming. There is both industrial and academic interest in the problem of (semi-)automatically translating c code to Rust in order to exploit Rust's safety guarantees. We study the effectiveness and limitations of existing techniques for automatically translating unsafe raw pointers (in Rust programs translated from c) into safe Rust references via ownership and lifetime inference. Our novel evaluation methodology enables our study to extend beyond prior studies, and to discover new information contradicting the conclusions of prior studies. We find that existing translation methods are severely limited by a lack of precision in the Rust compiler's safety checker, causing many safe pointer manipulations to be labeled as potentially unsafe. Leveraging this information, we propose methods for improving translation, based on encoding the results of a more precise analysis in a manner that is understandable to an unmodified Rust compiler. We implement one of our proposed methods, increasing the number of pointers that can be translated to safe Rust references by 75% over the baseline (from 12% to 21% of all pointers).

关键词： Rust c Translation Memory Safety Empirical Study

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A Preliminary Study in Immune Response of BALB/c and c57BL/6 Mice with a Locally Allergic Rhinitis Model

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AMERIcAN JOURNAL OF RHINOLOGY & ALLERGY 2023年第4期37卷 410-418页

作者： Zhang, Qidi Zhu, Wanting Zou, Zhixin Yu, Wenting Gao, Pei Wang, Ying chen, Jianjun Huazhong Univ Sci & Technol Union Hosp Dept Otorhinolaryngol Tongji Med Coll Wuhan Peoples R China Anhui Med Univ Dept Otorhinolaryngol Head & Neck Surg Affiliated Hosp 1 Hefei Peoples R China

Background BALB/c and c57BL/6 mouse strains are commonly used in allergy research. The current study investigated the immunological differences between these two mouse strains with a locally allergic rhinitis model. Methods Eighteen BALB/c and eighteen c57BL/6 mice received different doses of ovalbumin (OVA) intranasally for eight weeks (each mouse strain has three subgroups, 25 mg/mL group, 0.25 mg/mL group, and the PBS group). The allergic symptoms, OVA-specific serum antibody (IgE, IgG1, IgG2a), cytokines (IL-4, IFN-gamma, IL-10) in the splenic culture supernatant, infiltrating eosinophils and goblet cells in local nasal mucosa were measured. RNA-seq technology was applied to detect differential gene expression in the local nasal mucosa. Results With the same dose of OVA stimulation, the exacerbation of allergic symptoms was more pronounced in c57BL/6 than in BALB/c. BALB/c serum IgE, IgG1, and IgG2a gradually increased, and c57BL/6 produced fewer serum antibodies IgE and IgG1, while IgG2a never increased. BALB/c spleen cell culture supernatant IL-4 and IL-10 increased with increasing dose, and IFN-gamma increased significantly in the intermediate dose group, while IL-4, IL-10, and IFN-gamma did not increase in c57BL/6. The infiltration of eosinophils and goblet cells in both mice was proportional to the dose, while c57BL/6 was elevated more than BALB/c. RNA-seq suggested that the innate immune response, immune system process function, Jun kinase (JNK) pathway, and MAPKK pathway were upregulated in c57BL/6 compared to BALB/c. The core genes responsible for the differential immune response in both mice with allergic rhinitis were Kng2, Kng1, Gnb3, Lpar3, Lpar1, Pik3r1, Pf4, Apob, Rps9, and Fbxo2. conclusion There are significant differences in the immunologic responses between BALB/c mice and c57BL/6 mice. BALB/c mice developed mild local allergic inflammatory reactions and strong systemic immune responses. In contrast, c57BL/6 mice had stronger local allergic inf

关键词： allergic rhinitis BALB c c57BL 6 animal model IgE IgG1 cytokines ovalbumin splenic culture supernatant RNA-seq

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