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Long-term exposure to 6-PPD quinone reduces reproductive capacity by enhancing germline apoptosis associated with activation of both DNA damage and cell corpse engulfment in caenorhabditis elegans

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JOURNAL OF HAZARDOUS MATERIALS 2023年 454卷 131495页

作者： Hua, Xin Feng, Xiao Liang, Geyu chao, Jie Wang, Dayong Southeast Univ Med Sch Key Lab Environm Med Engn Minist Educ Nanjing 210009 Peoples R China Southeast Univ Sch Publ Hlth Nanjing 210009 Peoples R China

Recently, 6-PPD quinone (6-PPDQ), a derivative of tire antioxidant 6-PPD, was reported to have acute toxicity for organisms. However, the possible reproductive toxicity of 6-PPDQ is still largely unclear. In this study, the repro-ductive toxicity of 6-PPDQ after long-term exposure was further investigated in caenorhabditis elegans. Exposure to 1 and 10 mu g/L 6-PPDQ reduced the reproductive capacity. Meanwhile, exposure to 1 and 10 mu g/L 6-PPDQ enhanced the germline apoptosis, which was accompanied by upregulation of ced-3, ced-4, and egl-1 expressions and down -regulation of ced-9 expression. The observed increase in germline apoptosis in 1 and 10 mu g/L 6-PPDQ exposed nematodes was associated with the enhancement in DNA damage and increase in expressions of related genes of cep -1, clk-2, hus-1, and mrt-2. The detected enhancement in germline apoptosis in 1 and 10 mu g/L 6-PPDQ exposed nematodes was further associated with the increase in expressions of ced-1 and ced-6 governing the cell corpse engulfment process. Molecular docking analysis indicated the binding potentials of 6-PPDQ with three DNA damage checkpoints (cLK-2, HUS-1, and MRT-2) and corpse-recognizing phagocytic receptor cED-1. Therefore, our data suggested the toxicity on reproductive capacity by 6-PPDQ at environmentally relevant concentrations by enhancing DNA damage-and cell corpse engulfment-induced germline apoptosis in organisms.

关键词： 6-PPDQ Reproductive toxicity Long-term exposure c elegans

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Achieving Good Bonding Strength of the cu Layer on PET Films by Pretreatment of a Mixed Plasma of carbon and copper

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AcS APPLIED MATERIALS & INTERFAcES 2023年第9期15卷 12590-12602页

作者： Li, Yue Wang, Yongxin Wang, Ying Wu, Yutao Chinese Acad Sci Ningbo Inst Mat Technol & Engn Key Lab Marine New Mat & Related Technol Zhejiang Key Lab Marine Mat & Protect Technol Ningbo 315201 Peoples R China Changzhou Univ Jiangsu Collaborat Innovat Ctr Photovolt Sci & Eng Sch Mat Sci & Engn Jiangsu Prov Cultivat Base State Key Lab Photovolt Changzhou 213164 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China

cu layers were fabricated on PET films with and without pretreatment by a mixed plasma composed of carbon and copper using a magnetron sputtering technique for potential application as the flexible copper-clad laminate (FccL) in 5G technology. In order to evaluate the effect of carbon plasma on the composited layer, the graphite target current was adjusted from 0.5 to 2.0 A. The microstructures and properties of cu layers on PET films with different treatments were measured by an X-ray powder diffractometer, X-ray photoelectron spectroscope, Raman spectro-scope, scanning electron microscope, transmission electron micro-scope, scratching test, indentation test, and four-probe detector. The results showed that the organic polymer carbon structure on the surface of PET films was changed to inorganic amorphous carbon due to the effect of the carbon plasma. At the same time, the active free radicals formed in the transition process react with metal copper ions to form organometallic compounds. Under the treatment of a mixed plasma of carbon and copper, the c/cu mixed layer was formed on the PET film at the top of the substrate. Due to the presence of c/cu mixed interlayers, the bonding strengths between the final cu layers and the PET film substrates were improved, and the strongest bonding strength appeared when the graphite target current was 1.0 A. In addition, the presence of the c/cu mixed interlayer enhanced the toughness of the cu layer on PET film. It was proposed that the good bonding strength in combination and the enhanced toughness for the cu layer on a PET film was due to the formation of a c/cu mixed interlayer induced by the pretreatment of a mixed plasma of carbon and copper.

关键词： copper-clad plate PET film pretreatment c cu layer bonding strength flexibility

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characterization of betalain-loaded liposomes and its bioactive potential in vivo after ingestion

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FOOD cHEMISTRY 2023年 407卷 135180页

作者： Martinez-Rodriguez, Pedro Alejandra Guerrero-Rubio, M. Hernandez-Garcia, Samanta Henarejos-Escudero, Paula Garcia-carmona, Francisco Gandia-Herrero, Fernando Univ Murcia Dept Bioquim & Biol Mol A Unidad Docente Biol Fac Vet Reg Campus Int Excellence Campus Mare Nos Murcia Spain Univ Cambridge Dept Plant Sci Cambridge CB2 3EA England

Betalains are plant pigments characterized by showing a wide range of beneficial properties for health. Its bioactive potential has been studied for the first time after its encapsulation in liposomes and subsequent administration to the animal model caenorhabditis elegans. Phenylalanine-betaxanthin and indoline carboxylic acid-betacyanin encapsulated at concentrations of 25 and 500 mu M managed to reduce lipid accumulation and oxidative stress in the nematodes. Highly antioxidant betalains dopaxanthin and betanidin were also included in the survival analyses. The results showed that phenylalanine-betaxanthin was the most effective betalain by increasing the lifespan of c. elegans by 21.8%. In addition, the administration of encapsulated natural betanidin increased the nematodes' survival rate by up to 13.8%. The preservation of the bioactive properties of betalains manifested in this study means that the stabilization of the plant pigments through encapsulation in liposomes can be postulated as a new way for administration in pharmacological and food applications.

关键词： Antilipidemic Antioxidant Betalain c elegans In vivo Liposome

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A natural blue colorant from butterfly pea (clitoria ternatea) petals for traditional rice cooking

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JOURNAL OF FOOD ScIENcE AND TEcHNOLOGY-MYSORE 2023年第8期60卷 2255-2264页

作者： Maneechot, Oranit Hahor, Waraporn Thongprajukaew, Karun Nuntapong, Nutt Bubaka, Samsiyah Prince Songkla Univ Fac Sci Div Hlth & Appl Sci Hat Yai 90110 Thailand Prince Songkla Univ Fac Nat Resources Aquat Sci & Innovat Management Div Hat Yai 90110 Thailand

Butterfly pea (clitoria ternatea) flowers are widely used for culinary purposes in southern Asian countries. We assessed the optimal level of a butterfly pea petal extract (BPPE) to produce blue rice. Dried butterfly pea petals were extracted with water at 0.2, 0.4, 0.6, and 0.8% (w/v), and the extract was used to color boiled rice before cooking was completed. Rice cooked with BPPE acquired various shades of blue from light to dark. Significantly decreased lightness/darkness (L*), redness/greenness (a*), yellowness/blueness (b*), and color difference ( increment E*) were observed in response to BPPE levels, while hue (h*) and chroma (c*) were significantly increased (p < 0.05). Total phenolic (TPc) and total anthocyanin (TAc) contents of cooked rice increased with BPPE levels (p < 0.05), and both constituents contributed significant radical scavenging activity (r = 0.886 to 0.994, p < 0.01, n = 20). Sensory evaluation by consumers indicated a suitable level of BPPE at 0.6%. The color of blue cooked rice was stable under an opaque cover for 8 days, whereas TPc and TAc decreased significantly with storage time. Findings from the current study can be used to produce blue rice in traditional cooking, and to produce other ready-to-use BPPE products.

关键词： Anthocyanin Antioxidant Asian pigeonwings Blue rice c ternatea Phenolics

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Influence of a rich sulphur atmosphere on the phase development and kinetic behaviour of differentially processed RNT650?- TiAl alloys

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JOURNAL OF MATERIALS RESEARcH AND TEcHNOLOGY-JMR&T 2023年 23卷 1753-1760页

作者： Dudziak, T. Rzad, E. Morgiel, J. Kirchner, A. Polczyk, T. Moskal, G. Kloeden, B. Weissgaerber, T. Tobola, D. Wojcicki, M. Krakow Inst Technol Lukasiewicz Res Network 73 Zakopianska Str PL-30418 Krakow Poland Polish Acad Sci Inst Met & Mat Sci 25 Reymonta Str PL-30059 Krakow Poland Inst Dresden Fraunhofer Inst Fertigungstech & Angew Mat Forsch Winterbergstr 28 D-01277 Dresden Germany Silesian Tech Univ Fac Mat Engn Dept Adv Mat & Technol 8 Krasinskiego Str PL-40019 Katowice Poland

The influence of sulphur atmosphere on conventionally cast (cM) and on electron beam melted (EBM) g -TiAl RNT650 alloy exposed to a 1% (vol.) H2S -air atmosphere at 650 degrees c for 500 h was investigated. The material produced by cM showed a lower mass gain than the EBM material. XRD and TEM/EDS analyses indicated the formation of rutile-TiO2/(a-Al2-O3 + rutile-TiO2)/amorphous-Al2O3 sequence of phases. In EBM a coarse crystalline layer rich in aluminium, oxygen and a small amount of sulphur was found. The higher content of sulphur was in the scale bottom intermixed with amorphous alumina.(c) 2023 Published by Elsevier B.V. This is an open access article under the cc BY-Nc-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： A intermetallics Titanium B XRD TEM c oxidation Sulphidation

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Developing a high-efficiency predictive model for self-temperature-compensated piezoresistive properties of carbon nanotube/graphene nanoplatelet polymer-based nanocomposites

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cOMPOSITES PART A-APPLIED ScIENcE AND MANUFAcTURING 2023年 166卷

作者： Haghgoo, Mojtaba Ansari, Reza Jang, Sung-Hwan Hassanzadeh-Aghdam, Mohammad Kazem Nankali, Mohammad Univ Guilan Fac Mech Engn Rasht Iran Hanyang Univ ERICA Sch Engn Dept Civil & Environm Engn Ansan 15588 Gyeonggi Do South Korea Univ Guilan Fac Technol & Engn Dept Engn Sci Rudsar Vajargah Iran Iran Univ Sci & Technol Dept Mech Engn Tehran Iran

The thermo-resistive and piezoresistive responses of carbon nanotube (cNT)/graphene nanoplatelet (GNP) polymer-based nanocomposites are analytically investigated. The 3D representative volume element is generated by the Monte carlo approach to incorporate the random distribution of nanofillers. The Monte carlo approach is paired with the percolation model to investigate the percolation behavior of the nanocomposite. The validity of the analytical model is verified by comparing the predicted results with the experimental data. The Poisson's ratio and height of barrier potential influence on the piezoresistivity of nanocomposite are studied. Analytical results determine the aspect ratio and influence of carbon nanotube degree of orientation on thermoresistivity of nanocomposite. The effects of intrinsic and physical properties of GNPs on resistivity change with temperature are investigated. It is found that nanocomposite filled with cNTs presented lower percolation threshold than those filled with GNPs. The results also revealed that the filler alignment caused a higher piezoresistivity.

关键词： A Multifunctional composites B Hybrid c Electrical properties D Analytical modeling

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7-Ethoxycoumarin rescued caenorhabditis elegans from infection of cOPD derived clinical isolate Pseudomonas aeruginosa through virulence and biofilm inhibition via targeting Rhl and Pqs quorum sensing systems

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WORLD JOURNAL OF MIcROBIOLOGY & BIOTEcHNOLOGY 2023年第8期39卷 1-15页

作者： Bajire, Sukesh Kumar Prabhu, Ashwini Bhandary, Yashodhar P. Irfan, K. M. Shastry, Rajesh P. Yenepoya Yenepoya Res Ctr Div Microbiol & Biotechnol Univ Rd Mangaluru 575018 India Yenepoya Yenepoya Res Ctr Univ Rd Mangaluru 575018 India Yenepoya Med Coll Hosp Dept Pulm Med Mangaluru 575018 India

Pseudomonas aeruginosa is an ambidextrous Gram-negative contagium with density convoluted network defined quorum sensing, which enables the persistent survival within the host environment, contributing to various lung related diseases including chronic Obstructive Pulmonary Disease (cOPD). It is clear that P. aeruginosa is a powerful, exquisite pathogen that has adopted a variety of virulence properties through quorum sensing (QS) regulated phenomenon and that it dominates both in the development and exacerbations of cOPD. Interestingly, 7-Ethoxycoumarin (7-Ec), a compound that adequately mimics QS signaling molecule of P. aeruginosa, was introduced as part of the process of developing novel ways to treat the severe exacerbations. The results showed that, introduction of 7-Ec significantly decreased exopolysaccharide-mediated biofilm development of strains isolated from cOPD sputum, as evidenced by SEM analysis. Furthermore, 7-Ec was able to modulate a variety of virulence factors and motility without subjecting planktonic cells to any selection pressure. Bacterial invasion assay revealed the potential activity of the 7-Ec in preventing the active entry to A549 cells without causing any damage to the cells and found functionally active in protecting the c. elegans from P. aeruginosa infection and being non-toxic to the worms. Docking analysis was further proved that 7-Ec to be the potential anti-QS compound competing specifically with Rhl and Pqs Systems. Therefore, 7-Ec in the utilisation against the P. aeruginosa based infections, may open an avenue for the futuristic mechanistic study in chronic respiratory diseases and a initiator for the development of non-antibiotic based antibacterial therapy.

关键词： chronic Obstructive Pulmonary Disease Quorum sensing P aeruginosa c elegans Virulence factors Biofilm

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See the Unseen: Red-Emissive carbon Dots for Visualizing the Nucleolar Structures in Two Model Animals and In Vivo Drug Toxicity

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SMALL 2023年第31期19卷 e2205890页

作者： Xu, Ke-Fei Jia, Hao-Ran Wang, Zihao Feng, Hui-Heng Li, Ling-Yi Zhang, Rufeng Durrani, Samran Lin, Fengming Wu, Fu-Gen Southeast Univ Sch Biol Sci & Med Engn State Key Lab Bioelect 2 Sipailou Rd Nanjing 210096 Peoples R China

Nucleolus, which participates in many crucial cellular activities, is an ideal target for evaluating the state of a cell or an organism. Here, bright red-emissive carbon dots (termed cPcDs) with excitation-independent/polarity-dependent fluorescence emission are synthesized by a one-step hydrothermal reaction between congo red and p-phenylenediamine. The cPcDs can achieve wash-free, real-time, long-term, and high-quality nucleolus imaging in live cells, as well as in vivo imaging of two common model animals-zebrafish and caenorhabditis elegans (c. elegans). Strikingly, cPcDs realize the nucleolus imaging of organs/flowing blood cells in zebrafish at a cellular level for the first time, and the superb nucleolus imaging of c. elegans suggests that the germ cells in the spermatheca probably have no intact nuclei. These previously unachieved imaging results of the cells/tissues/organs may guide the zebrafish-related studies and benefit the research of c. elegans development. More importantly, a novel strategy based on cPcDs for in vivo toxicity evaluation of materials/drugs (e.g., Ag+), which can visualize the otherwise unseen injuries in zebrafish, is developed. In conclusion, the cPcDs represent a robust tool for visualizing the structures and dynamic behaviors of live zebrafish and c. elegans, and may find important applications in cell biology and toxicology.

关键词： c elegans carbon nanodots in vivo toxicity nucleolar imaging zebrafish

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Influence of citric Acid and Hydrochloric Acid with High-Pressure Processing on characteristics of Pectic Polysaccharide from choerospondias axillaris Fruit Peel

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FOOD AND BIOPROcESS TEcHNOLOGY 2023年第6期16卷 1235-1245页

作者： Li, Lu chen, Shupeng Lu, Jinghui Bu, Zhibin Yu, Yuanshan Wu, Jijun Yang, Wanyuan Xu, Yujuan Peng, Jian Guangdong Acad Agr Sci Sericultural & Argi Food Res Inst Minist Agr & Rural Affairs Guangdong Key Lab Agr Prod ProcKey Lab Funct Food 133 Yiheng StDongguanzhuang Rd Guangzhou 510610 Peoples R China Guangdong Jiabao Grp Co Ltd Chaozhou 515638 Guangdong Peoples R China

The aim of this study was to investigate the influence of citric acid combined with high-pressure processing (HPP) on the characteristics of pectic polysaccharide of choerospondias axillaris fruit peel (cPP). Therefore, the physicochemical, structural, and emulsifying properties of cPP extracted by hydrochloric acid (HEP), HPP-assisted hydrochloric acid (HEP + HPP), citric acid (cEP), and HPP-assisted citric acid (cEP + HPP) were analyzed. Results showed that citric acid and HPP had a synergetic impact on extraction yield, weight-average molecular weight (Mw), emulsion ability (EA), and apparent viscosity of cPP;hence, the highest extraction yield (48.49%) and EA (70.25%) coupled with the lowest Mw (263.80 kDa) and apparent viscosity were observed in cEP + HPP. However, the effects of citric acid and HPP on DA, DM, and GalA of cPP were opposite. The lowest DA and DM were found in HEP + HPP. Moreover, both citric acid and HPP had great influences on the structure of cPP. The cPP extracted by citric acid had a lower crystallinity, and the microstructure of cPP extracted with HPP assistance was rougher. In summary, HPP-assisted citric acid extraction could improve the extraction yield and emulsion property of cPP.

关键词： c axillaris fruit peel Pectic polysaccharide Extraction method Physicochemical characteristics Structural properties Emulsifying quality

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Using Pgst-4::GFP-transformed caenorhabditis elegans for drinking water quality monitoring

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ENVIRONMENTAL MONITORING AND ASSESSMENT 2023年第6期195卷 1-15页

作者： Wang, Yan Han, Xue Zhang, Jie Zhou, Meng-Xuan Liu, Ai-Lin Huazhong Univ Sci & Technol Minist Educ Key Lab Environm & Hlth Wuhan 430030 Peoples R China Huazhong Univ Sci & Technol Minist Environm Protect Wuhan 430030 Peoples R China Huazhong Univ Sci & Technol Tongji Med Coll Sch Publ Hlth State Key Lab Environm Hlth Incubating Wuhan 430030 Peoples R China Henan Univ Chinese Med Zhengzhou 450046 Peoples R China Wuhan Water Grp Co Ltd Ctr Water Qual Monitoring Wuhan 430034 Peoples R China

Biological effect-based monitoring is essential for predicting or alerting to a possible deterioration in drinking water quality. In the present study, a reporter gene assay based on oxidative stress-mediated Pgst-4::GFP induction in the caenorhabditis elegans strain VP596 (VP596 assay) was assessed for its applicability in evaluating drinking water safety and quality. This assay was used to measure the oxidative stress response in VP596 worms exposed to six ubiquitous components (As3+, Al3+, F-, NO3--N, cHcl3, and residual chlorine) in drinking water, eight mixtures of these six components designed through orthogonal design, ninety-six unconcentrated water samples from source to tap water in two supply systems, and organic extracts (OEs) of twenty-five selected water samples. Pgst-4::GFP fluorescence was not induced by Al3+, F-, NO3--N, and cHcl3, and was significantly enhanced by As3+ and residual chlorine only at concentrations higher than their respective drinking water guideline levels. Pgst-4::GFP induction was not detected in any of the six-component mixtures. Induction of Pgst-4::GFP was observed in 9.4% (3/32) of the source water samples but not in the drinking water samples. However, a notable induction effect was revealed in the three OEs of drinking water, with a relative enrichment factor of 200. These results suggest that the VP596 assay has limited utility for screening drinking water safety by testing unconcentrated water samples;however, it offers a supplemental in vivo tool for prioritizing water samples for an enhanced quality assessment, monitoring pollutant removal performance by drinking water treatment plants, and evaluating water quality in water supplies.

关键词： Drinking water Water quality Safety screening Biomonitoring Oxidative stress c elegans

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