The rhizome of Gastrodia elata(GE), a herb medicine, has been used for treatment of neuronal disorders in Eastern Asia for hundreds of years. Parishin c is a major ingredient of GE. In this study, the i.c.v. injection...
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The rhizome of Gastrodia elata(GE), a herb medicine, has been used for treatment of neuronal disorders in Eastern Asia for hundreds of years. Parishin c is a major ingredient of GE. In this study, the i.c.v. injection of soluble Aβ1–42oligomers model of LTP injury was used. We investigated the effects of parishin c on the improvement of LTP in soluble Aβ1–42oligomer–injected rats and the underlying electrophysiological mechanisms. Parishin c(i.p. or i.c.v.) significantly ameliorated LTP impairment induced by i.c.v. injection of soluble Aβ1–42oligomers. In cultured hippocampal neurons,soluble Aβ1–42oligomers significantly inhibited NMDAR currents while not affecting AMPAR currents and voltage-dependent currents. Pretreatment with parishin c protected NMDA receptor currents from the damage induced by Aβ. In summary, parishin c improved LTP deficits induced by soluble Aβ1–42oligomers. The protection by parishin c against Aβ-induced LTP damage might be related to NMDA receptors.
The aim of this study was to determine the feasibility of producing biogas by combining solid poultry slaughterhouse wastes (SPSWs) with fruit and vegetable solid wastes (FVSWs) and to obtain the optimum conditions fo...
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The aim of this study was to determine the feasibility of producing biogas by combining solid poultry slaughterhouse wastes (SPSWs) with fruit and vegetable solid wastes (FVSWs) and to obtain the optimum conditions for maximum biogas production by means of anaerobic digestion (AD). The ratio of available carbon to available nitrogen (c/N), as a critical parameter in producing methane, was adjusted by mixing the appropriate amount of SPSWs with FVSWs. For each load, a 50-L pilot-scale digester was operated for 40 days. For start-up and seeding, the biogas reactor was bioaugmented using the anaerobic sludge of an industrial poultry wastewater treatment plant. The analyzed SPSW, which had a c/N ratio of 5.75, was combined with FVSWs to obtain c/N ratios of 20, 30, and 40. Furthermore, it was found that the highest biogas yield occurred when the c/N ratio of the feed was 30. Also, for all three loading ratios, biogas production volumes were 215.1, 241, and 182.7 L, respectively, where methane's shares were 104.5, 120.6, and 91.7 L of the total gas volume that constituted 48.6, 50, and 50.2% of the produced biogas. Therefore, the obtained results from the process of mesophilic AD of this type of solid wastes illustrate that it is possible to efficiently produce biogas as a renewable energy source by combining SPSWs and FVSWs, instead of burning or burying them.
In the paper, the urchin-like TiO2 with rutile crystal structure was prepared under acidicconditions. When c powder was added in the reaction process, rutile TiO2 became rutile and anatase diphase structure. The yiel...
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In the paper, the urchin-like TiO2 with rutile crystal structure was prepared under acidicconditions. When c powder was added in the reaction process, rutile TiO2 became rutile and anatase diphase structure. The yields of cH4 and cO of c/Ag@TiO2 were 5.46 & mu;mol & BULL;g- 1 & BULL;h-1 and 1.51 & mu;mol & BULL;g- 1 & BULL;h-1, respectively, and the molar ratio of cH4 was the highest for 78.3%. Through characterization of optical and electrical properties, the addition of c and Ag improved the optical performance, carrier concentration, and electron mobility. Finally, the photo -catalytic reaction mechanism was proposed.
AIM To clone core gene cDNA of chinesehepatitis c virus(HcV)into eukaryoticexpression vector cosmid pTM3 and to expressHcV core antigen in HepG2 *** core gene cDNA of HcV wasintroduced into eukaryotic expression vecto...
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AIM To clone core gene cDNA of chinesehepatitis c virus(HcV)into eukaryoticexpression vector cosmid pTM3 and to expressHcV core antigen in HepG2 *** core gene cDNA of HcV wasintroduced into eukaryotic expression vectorcosmid *** vaccinia virus/bacteriophage T7 hybrid expression system,HepG2 cells were transfected with therecombinant plasmid pTM3-Q534 by *** From the transfected bacteriaTop10F’,2 pTM3-Q534 clones containing therecombinant plasmid were identified fromrandomly selected 10 *** reverse transcription PcR andindirect immunofluorescence technique,HcVRNA and core protein was identified in HepG2cells transfected with the recombinant *** The construction of arecombinant plasmid and the expression of coregene cDNA of HcV in HepG2 was successful.
The blending system of chitooligosaccharide (cOS) and polyethyleneimine (PEI) was studied as a drug carrier for tumor treatment. Nanoparticles cOS/PEI-PolyI:c-OVA-x (cP-P-O-x) (x=1, 2, 3) were prepared by electrostati...
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The blending system of chitooligosaccharide (cOS) and polyethyleneimine (PEI) was studied as a drug carrier for tumor treatment. Nanoparticles cOS/PEI-PolyI:c-OVA-x (cP-P-O-x) (x=1, 2, 3) were prepared by electrostatic self-assembly of cOS and PEI with the immune enhancing drug PolyI:c and the mimic antigen ovalbumin (OVA) using different feeding methods. The results showed that the nanoparticle solution could be stable only when the concentration of the added PEI was above 5.0% w/w. PolyI:ccould be coated well and protected from nuclease degradation. The OVA encapsulation efficiency was above 75%. The results of cell viability experiments showed that the blend of cOS and PEI had low cytotoxicity. The cP-P-O-1 had a suitable particle size, which was easy to be absorbed and expressed by cells. The results of in vitro immunization showed that due to the addition of PolyI:c, whether OVA was loaded on the inside or on the surface, nanoparticles significantly promoted the secretion of cytokines mouse tumor necrosis factor alpha (TNF-alpha) and mouse interferon-gamma (IFN-gamma). The feeding method mainly had a greater impact on the morphology and size of the nanoparticles, and had little effect on solution stability, OVA encapsulation efficiency, binding ability with PolyI:c, resistance to nuclease degradation and immune performance. cP-P-O-x prepared by the blend system of cOS and PEI will be a potential candidate for tumor treatment.
The fluorescence properties of c 60 in different organic solvents have been investigated at room temperature. Three fluorescence emission centers are discovered and ascribed to different aggregations of c 60 in solven...
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The fluorescence properties of c 60 in different organic solvents have been investigated at room temperature. Three fluorescence emission centers are discovered and ascribed to different aggregations of c 60 in solvent. A series of blue fluorescence peaks centered at 440 nm derive from c 60 nanoparticles; a distinctive yellow-green fluorescence band in 575 nm region arises from the aggregates of c 60 nanoparticles; a more informative salmon fluorescence band around 700 nm originates from c 60 microcrystals. And the distinct configurations of c 60 aggregations are closely associated with the characteristic interaction between c 60 and solvent molecules.
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FARRELL, KIstel Ltd.
PO Box 5 Grosvenor House Prospect Hill Redditch Worcs B97 4DQ UK
This paper details an experienced programmer's first impressions of working with the Unix operating system and c programming language. Use of the Unix shell, vi editor, ccompiler and some utilities are discussed,...
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This paper details an experienced programmer's first impressions of working with the Unix operating system and c programming language. Use of the Unix shell, vi editor, ccompiler and some utilities are discussed, as are the Unix documentation and some relevant textbooks. An assessment of the difficulties and advantages of starting with Unix is given.
In this study, pyrolysis of low-density polyethylene (LDPE) and LDPE with aluminum (c/LDPE) wastes was carried out with different heating rates (5, 10, 20 degrees c/min) at different temperatures (400, 600, 800 degree...
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In this study, pyrolysis of low-density polyethylene (LDPE) and LDPE with aluminum (c/LDPE) wastes was carried out with different heating rates (5, 10, 20 degrees c/min) at different temperatures (400, 600, 800 degrees c). Product yields of LDPE and c/LDPE wastes were compared, and optimum liquid products were analyzed to utilize as commercial waxes for future use. The properties of pyrolyzed wastes was investigated with proximate, elemental analysis, and TGA. The as-produced liquid from pyrolysis of wastes was characterized by different characteristic tools, such as elemental analyses, Gc-MS analyses, 1H-NMR tests, FT-IR spectra, the density, melting point, and carbon residue to compare commercial waxes. As a result of pyrolysis, the highest liquid product yield was achieved at 800 degrees c with 5 degrees c/min heating rate (85.87%) and at 600 degrees c with 5 degrees c/min heating rate (71.3%) for LDPE and c/LDPE, respectively. The results indicated that the derived liquid products are similar to commercial heavy wax.
Using the MEVVA ion source, carbon ions have been implanted in TiN coatings deposited by multi-arc ion plating The Vickers microhardness of the c+ -implanted TiN films increased with the increase in the ion flux and d...
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Using the MEVVA ion source, carbon ions have been implanted in TiN coatings deposited by multi-arc ion plating The Vickers microhardness of the c+ -implanted TiN films increased with the increase in the ion flux and dose. X-ray diffraction (XRD) analysis showed that the Tic phases had been formed in the films. In addition, the films had the preferred growth orientations of TiN and Tic, both of which were (111) orientation after annealing at 500℃ for 30 min. Auger electron spectra analysis indicated that c+ -implanted profile was in typical Gaussian-like distribution in single films. The distribution with multipeaks of c atoms was obtained in multi-layer TiN/Ti. The possibility of the multilayer films (Ti (c, N)/TiN/Ti(c, N)/TiN and Ti(c, N)/Tic/Ti(c, N)/Tic) forming using the c-implanted TiN/Ti films is presented for the first time.
The mechanism and effect of c/N ratios on the aerobic granules simultaneous nitrification, denitrification and phosphorus removal (SNDPR) system are still unclear. The reactor performance and microbial community dynam...
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The mechanism and effect of c/N ratios on the aerobic granules simultaneous nitrification, denitrification and phosphorus removal (SNDPR) system are still unclear. The reactor performance and microbial community dynamics of the system were investigated under variable c/N ratios (20, 10 and 5). The cOD, TP and NH4+-N removal remained unaffected with variable c/N ratios. The decreased c/N ratio of five strongly influenced the nitrogen removal. Further investigations revealed that candidatus_Accumulibacter, Acinetobacter, candidatus_competibacter were the predominant genera. classification of key groups involved in nitrogen and phosphorus removal indicated the lowest c/N ratio resulting in a large microbial community shift. This study might contribute to the application of SNDPR system for the treatment of wastewater. Different c/N ratios led to shift on the microbial community and the dominant was phosphorus-accumulating bacteria. The nitrogen removal efficiency decreased while the removal of cOD, TP and NH4+-N remained remarkable with the decreased c/N ratios.
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