检索结果-内蒙古大学图书馆

comparative mRNA analysis of behavioral and genetic mouse models of aggression

AMERIcAN JOURNAL OF MEDIcAL GENETIcS PART B-NEUROPSYcHIATRIc GENETIcS 2016年第3期171卷 427-436页

作者： Malki, Karim Tosto, Maria G. Pain, Oliver Sluyter, Frans Mineur, Yann S. crusio, Wim E. de Boer, Sietse Sandnabba, Kenneth N. Kesserwani, Jad Robinson, Edward Schalkwyk, Leonard c. Asherson, Philip Kings Coll London MRC Social Genet & Dev Psychiat Ctr Inst Psychiat London WC2R 2LS England Tomsk State Univ Lab Cognit Invest & Behav Genet Tomsk 634050 Russia Univ London Birkbeck Ctr Brain & Cognit Dev London England London Sch Hyg & Trop Med Dept Noncommunicable Dis Epidemiol London WC1 England Yale Univ Sch Med Dept Psychiat New Haven CT USA Univ Bordeaux Aquitaine Inst Cognit & Integrat Neurosci Bordeaux France CNRS Aquitaine Inst Cognit & Integrat Neurosci Bordeaux France Univ Groningen Groningen Inst Evolutionary LifeSci GELIFES Groningen Netherlands Abo Akad Univ Fac Arts Psychol & Theol Turku Finland Univ Essex Sch Biol Sci Colchester CO4 3SQ Essex England

Mouse models of aggression have traditionally compared strains, most notably BALB/cJ and c57BL/6. However, these strains were not designed to study aggression despite differences in aggression-related traits and distinct reactivity to stress. This study evaluated expression of genes differentially regulated in a stress (behavioral) mouse model of aggression with those from a recent genetic mouse model aggression. The study used a discovery-replication design using two independent mRNA studies from mouse brain tissue. The discovery study identified strain (BALB/cJ and c57BL/6J) x stress (chronic mild stress or control) interactions. Probe sets differentially regulated in the discovery set were intersected with those uncovered in the replication study, which evaluated differences between high and low aggressive animals from three strains specifically bred to study aggression. Network analysis was conducted on overlapping genes uncovered across both studies. A significant overlap was found with the genetic mouse study sharing 1,916 probe sets with the stress model. Fifty-one probe sets were found to be strongly dysregulated across both studies mapping to 50 known genes. Network analysis revealed two plausible pathways including one centered on the UBc gene hub which encodes ubiquitin, a protein well-known for protein degradation, and another on P38 MAPK. Findings from this study support the stress model of aggression, which showed remarkable molecular overlap with a genetic model. The study uncovered a set of candidate genes including the Erg2 gene, which has previously been implicated in different psychopathologies. The gene networks uncovered points at a Redox pathway as potentially being implicated in aggressive related behaviors. (c) 2016 Wiley Periodicals, Inc.

关键词： aggression mouse BALB c transcriptomics

来源：评论

学校读者我要写书评

暂无评论

Impact of micro-environmental changes on respiratory tract infections with intracellular bacteria

引用

FEBS LETTERS 2016年第21期590卷 3887-3904页

作者： Shima, Kensuke coopmeiners, Jonas Graspeuntner, Simon Dalhoff, Klaus Rupp, Jan Univ Lubeck Dept Infect Dis & Microbiol Ratzeburger Allee 160 D-23538 Lubeck Germany Univ Hosp Schleswig Holstein Med Clin 3 Campus Lubeck Lubeck Germany

community-acquired pneumonia is caused by intra- and extracellular bacteria, with some of these bacteria also being linked to the pathogenesis of chronic lung diseases, including asthma and chronic obstructive pulmonary disease. chlamydiapneumoniae is an obligate intracellular pathogen that is highly sensitive to micro-environmental conditions controlling both pathogen growth and host immune responses. The availability of nutrients, as well as changes in oxygen, pH and interferon- levels, have been shown to directly influence the chlamydial life cycle and clearance. Although the lung has been traditionally regarded as a sterile environment, sequencing approaches have enabled the identification of a large number of bacteria in healthy and diseased lungs. The influence of the lung microbiota on respiratory infections has not been extensively studied so far and data on chlamydial infections are currently unavailable. In the present study, we speculate on how lung microbiota might interfere with acute and chronic infections by focusing exemplarily on the obligate intracellular ***. Furthermore, we consider changes in the gut microbiota as an additional player in the control of lung infections, especially in view the increasing evidence suggesting the involvement of the gut microbiota in various immunological processes throughout the human body.

关键词： anaerobic bacteria c pneumonia chronic infection cOPD lung microbiome micro-environment respiratory tract infection ScFAs

来源：评论

学校读者我要写书评

暂无评论

Identification and characterization of LIN-2(cASK) as a Regulator of Kinesin-3 UNc-104(KIF1A) Motility and clustering in Neurons

引用

TRAFFIc 2016年第8期17卷 891-907页

作者： Wu, Gong-Her Shanmugam, Muniesh Muthaiyan Bhan, Prerana Huang, Yu-Hsin Wagner, Oliver Ingvar Natl Tsing Hua Univ Inst Mol & Cellular Biol Hsinchu 30013 Taiwan Natl Tsing Hua Univ Dept Life Sci Hsinchu 30013 Taiwan

Kinesin-3 UNc-104(KIF1A) is the major axonal transporter of synaptic vesicles. Employing yeast two-hybrid and co-immunoprecipitation (co-IP) assays, we characterized a LIN-2(cASK) binding site overlapping with that of reported UNc-104 activator protein SYD-2(Liprin-) on the motor's stalk domain. We identified the L27 and GUK domains of LIN-2 to be the most critical interaction domains for UNc-104. Further, we demonstrated that the L27 domain interacts with the sterile alpha motifs (SAM) domains of SYD-2, while the GUK domain is able to interact with both the coiled coils and SAM domains of SYD-2. LIN-2 and SYD-2 colocalize in caenorhabditis elegans neurons and display interactions in bimolecular fluorescence complementation (BiFc) assays. UNc-104 motor motility and Synaptobrevin-1 (SNB-1) cargo transport are largely diminished in neurons of LIN-2 knockout worms, which cannot be compensated by overexpressing SYD-2. The absence of the motor-activating function of LIN-2 results in increased motor clustering along axons, thus retaining SNB-1 cargo in cell bodies. LIN-2 and SYD-2 both positively affect the velocity of UNc-104, however, only LIN-2 is able to efficiently elevate the motor's run lengths. From our study, we conclude that LIN-2 and SYD-2 act in a functional complex to regulate the motor with LIN-2 being the more prominent activator.

关键词： BiFc c elegans cASK KIF1A LIN-2 Liprin- MAGUK SNB-1 SYD-2 Synaptobrevin-1 UNc-104

来源：评论

学校读者我要写书评

暂无评论

脉冲占空比和脉冲频率对Ni-co-Sic复合涂层组织和性能的影响

引用

铸造技术 2017年第7期38卷 1615-1619页

作者：姚艳玲包头职业技术学院内蒙古包头014030

在碳钢X65上通过脉冲电流沉积的方法制备Ni-co-Sic复合涂层,研究了脉冲占空比和脉冲频率对涂层性能的影响。结果表明,随着脉冲占空比的减少和脉冲频率的增加,Ni-co-Sic复合涂层形态从结节变为针状结构,沉积在涂层中的Sic颗粒增加,晶粒... 详细信息

在碳钢X65上通过脉冲电流沉积的方法制备Ni-co-Sic复合涂层,研究了脉冲占空比和脉冲频率对涂层性能的影响。结果表明,随着脉冲占空比的减少和脉冲频率的增加,Ni-co-Sic复合涂层形态从结节变为针状结构,沉积在涂层中的Sic颗粒增加,晶粒尺寸变得更加细小。当显微硬度在5100N/mm^2和6700N/mm^2之间变化时,涂层的显微硬度随着脉冲占空比的增加而降低,随着频率的增加而增加。随着脉冲占空比的减小,电荷转移电阻值增加,复合涂层材料的耐腐蚀性增强;随着脉冲频率的增加,电荷转移电阻增加,复合涂层材料的耐腐蚀性增强。

关键词：脉冲电沉积 Ni-co-Si c 脉冲占空比脉冲频率

来源：评论

学校读者我要写书评

暂无评论

红树林内生真菌Aspergillus sp.085035次级代谢产物研究

引用

中药材 2017年第2期40卷 342-346页

作者：张柳红王嘉健洪葵林永成李静刘岚中山大学海洋科学学院广东广州510006 中山大学化学化工学院广东广州510275 武汉大学药学院湖北武汉430072 南海生物资源开发与利用协同创新中心广东广州510275

目的:研究红树林内生真菌Aspergillus sp.085035的次级代谢产物。方法:采用柱色谱和半制备高效液相色谱技术对Aspergillus sp.085035的次级代谢产物进行分离纯化,并利用波谱数据和X-射线单晶衍射等方法鉴定化合物结构;采用MTT法对所得... 详细信息

目的:研究红树林内生真菌Aspergillus sp.085035的次级代谢产物。方法:采用柱色谱和半制备高效液相色谱技术对Aspergillus sp.085035的次级代谢产物进行分离纯化,并利用波谱数据和X-射线单晶衍射等方法鉴定化合物结构;采用MTT法对所得化合物进行细胞毒活性检测。结果:从红树林内生真菌Aspergillus sp.085035的乙酸乙酯粗提物中分离得到9个化合物,分别鉴定为:13R-fumigaclavine c(1)、8'-O-methylasterric acid(2)、1,2-secotrypacidin(3)、rhizoctonic acid(4)、chaetominine(5)、helvolic acid(6)、trypacidin(7)、emodin-8-methylether(8)和fumitremorgin c(9)。结论:化合物1为新天然产物,化合物1、8为首次从曲霉属中分离得到,所有化合物均为首次从该真菌中分离得到,化合物7对MDA-MB-435细胞株具有细胞毒活性。

关键词：红树林内生真菌次级代谢产物 Aspergillus sp.085035 13R-fumigaclavine c

来源：评论

学校读者我要写书评

暂无评论

Role of cystatin c and renal resistive index in assessment of renal function in patients with liver cirrhosis

引用

World Journal of Gastroenterology 2014年第21期20卷 6573-6579页

作者： Dorde culafi Milos tuli Radmila Obrenovi Danijela Mileti Dragana Mija Milica Stojkovi Marija Jovanovi Milica culafi Clinic of Gastroenterology and Hepatology Clinical Center of Serbia and School of Medicine University of Belgrade Institute of Medical Biochemistry Clinical Center of Serbia Department of Pharmacoki-netics and Clinical Pharmacy Faculty of Pharmacy University of Belgrade

AIM:To evaluate the clinical significance of cystatin c and renal resistive index for the determination of renal function in patients with liver ***:We conducted a study of 63 patients with liver cirrhosis.A control group comprised of 30 age and gender-matched healthy *** cystatin c was determined in all study subjects and renal Doppler ultrasonography was *** glomerular filtration rate from serum creatinine(GFRcr)and cystatin c(GFRcys)was ***:We confirmed significant differences in val-ues of cystatin c between patients with different stages of liver cirrhosis according to child-Pugh(P=0.01),and a significant correlation with model of end stage liver disease(MELD)score(rs=0.527,P<0.001).More patients with decreased glomerular filtration rate were identified based on GFRcys than on GFRcr(P<0.001).Significantly higher renal resistive index was noted in child-Pugh c than in A(P<0.001)and B stage(P=0.001).Also,a significant correlation between renal resistive index and MELD score was observed(rs=0.607,P<0.001).Renal resistive index correlated significantly with cystatin c(rs=0.283,P=0.028)and showed a negative correlation with GFRcys(rs=-0.31,P=0.016).cONcLUSION:cystatin c may be a more reliable marker for assessment of liver ***,cystatin c and renal resistive index represent sensitive indicators of renal dysfunction in patients with liver cirrhosis.

关键词： Liver cirrhosis cystatin c Renal resistive index

来源：评论

学校读者我要写书评

暂无评论

N-Ethyl-N-Nitrosourea (ENU) Mutagenesis Reveals an Intronic Residue critical for caenorhabditis elegans 3 Splice Site Function in Vivo

引用

G3-GENES GENOMES GENETIcS 2016年第6期6卷 1751-1756页

作者： Itani, Omar A. Flibotte, Stephane Dumas, Kathleen J. Guo, chunfang Blumenthal, Thomas Hu, Patrick J. Univ Michigan Inst Gerontol Sch Med 3027 BSRB109 Zina Pitcher Pl Ann Arbor MI 48109 USA Univ Michigan Dept Internal Med Sch Med 3027 BSRB109 Zina Pitcher Pl Ann Arbor MI 48109 USA Univ Michigan Dept Cell & Dev Biol Sch Med 3027 BSRB109 Zina Pitcher Pl Ann Arbor MI 48109 USA Univ British Columbia Dept Zool Vancouver BC V6T 123 Canada Univ Colorado Dept Mol Cellular & Dev Biol Boulder CO 80309 USA Univ Washington Sch Med Dept Anesthesiol & Pain Med Seattle WA 98105 USA Buck Inst Res Aging Novato CA 94945 USA

Metazoan introns contain a polypyrimidine tract immediately upstream of the AG dinucleotide that defines the 3 splice site. In the nematode caenorhabditis elegans, 3 splice sites are characterized by a highly conserved UUUUcAG/R octamer motif. While the conservation of pyrimidines in this motif is strongly suggestive of their importance in pre-mRNA splicing, in vivo evidence in support of this is lacking. In an N-ethyl-N-nitrosourea (ENU) mutagenesis screen in caenorhabditis elegans, we have isolated a strain containing a point mutation in the octamer motif of a 3 splice site in the daf-12 gene. This mutation, a single base T-to-G transversion at the -5 position relative to the splice site, causes a strong daf-12 loss-of-function phenotype by abrogating splicing. The resulting transcript is predicted to encode a truncated DAF-12 protein generated by translation into the retained intron, which contains an in-frame stop codon. Other than the perfectly conserved AG dinucleotide at the site of splicing, G at the -5 position of the octamer motif is the most uncommon base in c. elegans 3 splice sites, occurring at closely paired sites where the better match to the splicing consensus is a few bases downstream. Our results highlight both the biological importance of the highly conserved -5 uridine residue in the c. elegans 3 splice site octamer motif as well as the utility of using ENU as a mutagen to study the function of polypyrimidine tracts and other AU- or AT-rich motifs in vivo.

关键词： c elegans splicing 3 splice site polypyrimidine tract daf-12 ENU

来源：评论

学校读者我要写书评

暂无评论

Targeting inflammation in metabolic syndrome

引用

TRANSLATIONAL RESEARcH 2016年第1期167卷 257-280页

作者： Welty, Francine K. Alfaddagh, Abdulhamied Elajami, Tarec K. Harvard Univ Beth Israel Deaconess Med Ctr Sch Med Div Cardiol Boston MA 02215 USA

The metabolic syndrome (MetS) is comprised of a cluster of closely related risk factors, including visceral adiposity, insulin resistance, hypertension, high triglyceride, and low high-density lipoprotein cholesterol;all of which increase the risk for the development of type 2 diabetes and cardiovascular disease. A chronic state of inflammation appears to be a central mechanism underlying the pathophysiology of insulin resistance and MetS. In this review, we summarize recent research which has provided insight into the mechanisms by which inflammation underlies the pathophysiology of the individual components of MetS including visceral adiposity, hyperglycemia and insulin resistance, dyslipidemia, and hypertension. On the basis of these mechanisms, we summarize therapeutic modalities to target inflammation in the MetS and its individual components. current therapeutic modalities can modulate the individual components of MetS and have a direct anti-inflammatory effect. Lifestyle modifications including exercise, weight loss, and diets high in fruits, vegetables, fiber, whole grains, and low-fat dairy and low in saturated fat and glucose are recommended as a first line therapy. The Mediterranean and dietary approaches to stop hypertension diets are especially beneficial and have been shown to prevent development of MetS. Moreover, the Mediterranean diet has been associated with reductions in total and cardiovascular mortality. Omega-3 fatty acids and peroxisome proliferator-activated receptor alpha agonists lower high levels of triglyceride;their role in targeting inflammation is reviewed. Angiotensin-converting enzyme inhibitors, angiotensin receptor blockers, and aldosterone blockers comprise pharmacologic therapies for hypertension but also target other aspects of MetS including inflammation. Statin drugs target many of the underlying inflammatory pathways involved in MetS.

关键词： AA arachidonic acid ABcA1 adenosine triphosphate-binding cassette transporter A1 AcE-I angiotensin-converting enzyme inhibitor AcSL1 acyl-coA synthase 1 ADMA asymmetric dimethylarginine Akt protein kinase B Aldo aldosterone AMPK adenosine monophosphate-activated protein kinase ANGPTL angiopoietin-like protein Ang angiotensin AngII angiotensin II AP-1 activator protein 1 Apo apolipoprotein ARB angiotensin receptor blocker Aspirin-cOX-2 aspirin-acetylated cyclooxygenase-2 AT-LX aspirin-triggered lipoxins AT-PD1 aspirin-triggered protectins AT-RvD aspirin-triggered resolvins c cholesterol cAD coronary artery disease cD cluster of differentiation cE cholesterol ester cETP cE transfer protein coQ10 coenzyme Q10 cOX cyclooxygenase cOX-2 cyclooxygenase-2 cRP c-reactive protein c-Src tyrosine-protein kinase c-Src Kinase cVD cardiovascular disease DAG diacylglycerides DHA docosahexaenoic acid EF ejection fraction eNOS endothelial nitric oxide synthase EPA eicosapentaenoic acid ET endothelin FetA Fetuin A FFA free fatty acids FoxO1 forkhead box protein O1 Glu glucose GLUT-4 glucose transporter type 4 GPR G-protein–coupled receptor GRK2 G-protein–coupled receptor kinase 2 HDL high-density lipoprotein HL hepatic lipase HR hazard ratio IcAM-1 intercellular adhesion molecule-1 IDL intermediate-density lipoprotein IKK inhibitor of nuclear factor kappa-B kinase IL interleukin ILR interleukin receptor iNOS inducible nitric oxide synthase LT leukotriene IRS insulin receptor substrate IκB inhibitor of nuclear factor kappa-B JNK c-Jun N-terminal kinase LOX lipoxygenase LPL lipoprotein lipase LX lipoxin MAPK Mitogen-activated protein kinases McP-1 monocyte chemoattractant protein-1 MetS metabolic syndrome MI myocardial infarction MMP matrix metalloproteinase MTP microsomal triglyceride transfer protein NF-κB nuclear factor kappa-B NLR nod-like receptor NO nitric oxide Omega-3 FAs omega-3 fatty acids oxLDL oxidized low-density lipoprotein PAI-1 plasminogen activator inhibitor-1 PD1 protectins PG prostaglandins PI3K phosphatidylinosito

来源：评论

学校读者我要写书评

暂无评论

creating a Transducer Electronic Datasheet using I2c Serial EEPROM Memory and PIc32-Based Microcontroller Development Board 7

引用

7th International conference on Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies (ATOM-N)

作者： croitoru, Bogdan Tulbure, Adrian Abrudean, Mihail Secara, Mihai 1 Decembrie 1918 Univ Alba Iulia Dept Precise & Engn Sci Alba Iulia Romania Tech Univ Cluj Napoca Dept Automat Cluj Napoca Romania

ISBN: (纸本)9781628413250

The present paper describes a software method for creating / managing one type of Transducer Electronic Datasheet (TEDS) according to IEEE 1451.4 standard in order to develop a prototype of smart multi-sensor platform (with up to ten different analog sensors simultaneously connected) with Plug & Play capabilities over ETHERNET and Wi-Fi. In the experiments were used: one analog temperature sensor, one analog light sensor, one PIc32-based microcontroller development board with analog and digital I/O ports and other computing resources, one 24Lc256 I2c (Inter Integrated circuit standard) serial Electrically Erasable Programmable Read Only Memory (EEPROM) memory with 32KB available space and 3 bytes internal buffer for page writes (1 byte for data and 2 bytes for address). It was developed a prototype algorithm for writing and reading TEDS information to / from I2c EEPROM memories using the standard c language (up to ten different TEDS blocks coexisting in the same EEPROM device at once). The algorithm is able to write and read one type of TEDS: transducer information with standard TEDS content. A second software application, written in VB. NET platform, was developed in order to access the EEPROM sensor information from a computer through a serial interface (USB).

关键词： IEEE 1451 smart sensors TEDS EEPROM microcontroller PIc embedded c Plug&Play

来源：评论

学校读者我要写书评

暂无评论

The Stress Granule RNA-Binding Protein TIAR-1 Protects Female Germ cells from Heat Shock in caenorhabditis elegans

引用

G3-GENES GENOMES GENETIcS 2016年第4期6卷 1031-1047页

作者： Huelgas-Morales, Gabriela Giovanni Silva-Garcia, carlos Salinas, Laura S. Greenstein, David Navarro, Rosa E. Univ Nacl Autonoma Mexico Inst Fisiol Celular Dept Biol Celular & Desarrollo Circuito Exterior S-NCiudad Univ Mexico City 04510 DF Mexico Univ Minnesota Dept Genet Cell Biol & Dev 4-208 MCB420 Washington Ave SE Minneapolis MN 55455 USA

In response to stressful conditions, eukaryotic cells launch an arsenal of regulatory programs to protect the proteome. One major protective response involves the arrest of protein translation and the formation of stress granules, cytoplasmic ribonucleoprotein complexes containing the conserved RNA-binding proteins TIA-1 and TIAR. The stress granule response is thought to preserve mRNA for translation when conditions improve. For cells of the germlinethe immortal cell lineage required for sexual reproductionprotection from stress is critically important for perpetuation of the species, yet how stress granule regulatory mechanisms are deployed in animal reproduction is incompletely understood. Here, we show that the stress granule protein TIAR-1 protects the caenorhabditis elegans germline from the adverse effects of heat shock. Animals containing strong loss-of-function mutations in tiar-1 exhibit significantly reduced fertility compared to the wild type following heat shock. Analysis of a heat-shock protein promoter indicates that tiar-1 mutants display an impaired heat-shock response. We observed that TIAR-1 was associated with granules in the gonad core and oocytes during several stressful conditions. Both gonad core and oocyte granules are dynamic structures that depend on translation;protein synthesis inhibitors altered their formation. Nonetheless, tiar-1 was required for the formation of gonad core granules only. Interestingly, the gonad core granules did not seem to be needed for the germ cells to develop viable embryos after heat shock. This suggests that TIAR-1 is able to protect the germline from heat stress independently of these structures.

关键词： stress granules TIA-1 TIAR germ cells stress c elegans

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：