In this work, buckypapers (BPs) were synthesised by different types of non-ionic surfactants. The prepared BPs were characterised by different methods. Using of non-ionic and also non-toxic surfactant, Tween 20, made ...
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In this work, buckypapers (BPs) were synthesised by different types of non-ionic surfactants. The prepared BPs were characterised by different methods. Using of non-ionic and also non-toxic surfactant, Tween 20, made the surface possible to immobilise the biological molecules on the surface for measuring the electrochemical properties changes. Moreover, this surface showed better electrochemical behaviour, higher conductivity, and better charge transfer kinetics than BP which was synthesised by conventional Triton X100. To our knowledge, this is the first research on the effect of surfactant type on the BP synthesis and characteristics.
In this work, a comparative analysis of vertical light-emitting diodes (VLEDs) examining the device properties is performed with different surface treatment schemes. The VLEDs on graphite substrate are fabricated by t...
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In this work, a comparative analysis of vertical light-emitting diodes (VLEDs) examining the device properties is performed with different surface treatment schemes. The VLEDs on graphite substrate are fabricated by the laser lift-off and the wafer bonding processes. The significance of KrF laser irradiation to form protrusions on the surfaces of undoped gallium nitride (u-GaN) and n-GaN is analysed in detail. The light output power of the fabricated VLEDs is significantly increased through the formation of micro-sized protrusions by the laser irradiation. For VLED irradiated with an energy density of 600 mJ/cm(2) on n-GaN surface with a thin u-GaN layer, the light output power is improved by 24.8% at 350 mA without degradation of electrical properties compared with that of the conventional VLED.
A method to measure the Amplitude and phase of the Fourier transform of the electron density function of complex materials is proposed. The method is based on the measurement of the interference between pairs of diffr...
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A method to measure the Amplitude and phase of the Fourier transform of the electron density function of complex materials is proposed. The method is based on the measurement of the interference between pairs of diffracted beams produced by two coherent non-collinear incident X-ray beams. The method is applicable to 2 dimensional crystals and 3 dimensional crystals with unit cells larger than about 80 angstrom. This method may open the way to a more direct determination of the structure of complex biological molecules.
A 8 MeV multicharged carbon or oxygen beam was used for Rutherford Backscattering Spectrometry (RBS) compositional analysis. Thin films of co, cu, Si, and Ni-cr of a few nm thickness were deposited on carbon substrate...
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A 8 MeV multicharged carbon or oxygen beam was used for Rutherford Backscattering Spectrometry (RBS) compositional analysis. Thin films of co, cu, Si, and Ni-cr of a few nm thickness were deposited on carbon substrates by vacuum evaporation or Ar sputtering. The measured results demonstrate that the mass resolution is much better for the heavy-ion RES than for the He-RES analysis. For the compositional analysis, a fitting function formula is proposed based on a simple model in which the thin film consists of many islands. The calculated results agree with the measured spectra, which show asymmetry of the peaks. Numerical analyses using the fitting function give reasonable agreement between the expected and measured ratios of mass elements in the films. (c) 1998 Published by Elsevier Science B.V.
Twenty-three patients with malignant lymphoma (13 cases of Hodgkin's disease and 10 cases of non-Hodgkin's Lymphoma) had their bone marrow studied based on a culture of medullary granulocytic progenitor cells ...
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Twenty-three patients with malignant lymphoma (13 cases of Hodgkin's disease and 10 cases of non-Hodgkin's Lymphoma) had their bone marrow studied based on a culture of medullary granulocytic progenitor cells (cFU c ) in methylcellulose. The number of cFU c per 10 5 nucleated medullary cells, which measures the medullary concentration in cFU c , has been established. These results also have been expressed in terms of the number of cFU c per 10 5 metamyelocytes plated, an index which measures the effectiveness of the leukopoiesis. Additionally, the number of cFU c per kg of body weight have been calculated. Before treatment, the number of cFU c is significantly lower than in normal subjects. There is a normal or elevated number of circulating granulocytes in these patients which can be explained by means of a mitotic amplification between the progenitor cell and the final differentiated cell. Ten patients were studied immediately after completion of radiotherapy. The number of cFU c returned from low to normal for 4 of them, remained low for 3 others, and was reduced from normal to below normal for the last 3. Four patients were studied between 6 and 8 months after radiotherapy. In all 4, the number of cFU c returned to normal, whether they had been initially lowered by radiotherapy or not. This normalization resulted from an increase in the concentration of cFU c in the non-irradiated zone, and for three of them resulted in part from a medullar regeneration in the irradiated zone.
Wastewater stabilization ponds (WSPs) rely on the metabolic activities of the inhabiting microbiota to treat wastewater. A selected consortium of chlorella vulgaris and chlorella protothecoides were used to manipulate...
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Wastewater stabilization ponds (WSPs) rely on the metabolic activities of the inhabiting microbiota to treat wastewater. A selected consortium of chlorella vulgaris and chlorella protothecoides were used to manipulate the natural resident microalgae assemblage to improve the treatment performance of a domestic wastewater pond treatment system in a coastal region. Since information is lacking about the resulting influence on the composition or succession of the phytoplankton or associated microbiota assemblage, the current study aimed to determine how dosing with the microalgae c. vulgaris and c. protothecoides change the efficiency of wastewater effluent treatment, as well as the composition and succession of the natural occurring phytoplankton and microbial assemblage throughout WSP system. After a year of specific microalgae inoculations, the effluent in part complied with the standards set by the Department of Water Affairs and Forestry (DWAF) and the USA, Environmental Protection Agency (EPA). The cyanobacteria Microcystis aeruginosa dominated the sixth (75%) and seventh pond (97%) before the inoculation with c. vulgaris and c. protothecoide commenced. After 12 inoculation events c. vulgaris and c. protothecoides were dominant in ponds three to seven while the dominant microbial groups were Bacteroidetes, cyanobacteria, Firmicutes, Planctomycetes, Proteobacteria, Spirochaetes, Synergistetes and Verrucomicrobia. After the microalgae treatment, the WSP effluent were more compliant regarding to the set guidelines for effluent than prior to microalgae treatment. Based on the ability of the c. vulgaris and c. protothecoides to improve the effluent water quality, it was evident that the consortium of microalgae can be use improve domestic wastewater effluent in rural nutrient sensitive catchments.
Nanomaterials synthesis using natural sources is the technology to up come with advanced materials through extracts of plant, microorganisms, poultry waste etc. In this study, the authors report the synthesis of porou...
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Nanomaterials synthesis using natural sources is the technology to up come with advanced materials through extracts of plant, microorganisms, poultry waste etc. In this study, the authors report the synthesis of porous carbon nanotubes using high-temperature decomposition technique facilitated by cobalt salt using chicken fats, a poultry waste as a precursor. Since chicken fats contain fatty acids which can decompose into short hydrocarbon chains and cobalt can act as the catalyst. The formation of carbon nanotubes was confirmed by Raman spectra, peaks at 1580 and 1350.46 cm(-1) confirmed the graphite mode G-band and structural imperfections defect mode D-band, respectively. Transmission electron microscopy showed the formation of tube-like structures. Nitrogen adsorption-desorption studies showed the high-surface area of 418.1 m(2)g(-1) with an estimated pore diameter of 8.1 nm. Thermogravimetry analysis-derivative thermogravimetric analysis-differential thermal analysis showed the instant weight loss at 517 degrees c attributed to the rapid combustion of nanotubes. A vibrating-sample magnetometer showed the paramagnetic nature of the so-formed carbon nanotubes formed.
OBJEcTIVE:In our study, we aimed to examine the effect of therapeutic hypothermia treatment on c-reactive protein (cRP) and interleukin-6 (IL-6) in infants with hypoxic ischemic encephalopathy (HIE).METHODS:The data o...
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OBJEcTIVE:In our study, we aimed to examine the effect of therapeutic hypothermia treatment on c-reactive protein (cRP) and interleukin-6 (IL-6) in infants with hypoxic ischemic encephalopathy (HIE).
METHODS:The data of the patients with the diagnosis of HIE we followed up in our unit between 2017 and 2018 were analyzed retrospectively. Patients who died during follow-up and patients with proven septicemia at the time of examination were excluded from the study. The routine cRP and IL-6 values of the patients included in the study were compared before and after hypothermia treatment.
RESULTS:Therapeutic hypothermia treatment applied for 72 hours was found to cause a statistically significant increase in cRP after treatment when compared with the values measured before treatment (0.6 (0.2-1.9) before and median (P25-75), and after treatment 7.5 (4-18) and median (P25-75) mg/L, p=0.00). While IL-6 was found to be high in the early period due to the effect of hypoxia, it was found to be low after hypothermia treatment (80.5 (40-200) median (P25-75) - 32 (18-50) median (P25-75) pg/ml, p=0.131). While the white blood cell count was high before hypothermia treatment due to hypoxia, it was found to be low after treatment (24600 (19600-30100) median (P25-75) -11300 (8800-14200) median (P25-75)/µL, p=0.001).
cONcLUSION:White blood cells and IL-6 can be found to be high due to hypoxia without infection, and cRP can be found to be high after therapeutic hypothermia treatment without infection. The effect of hypoxia and hypothermia should be considered when evaluating acute phase reactants.
candida albicans is a common opportunistic pathogen that causes a variety of diseases in immunocompromised hosts. In a pathogen, cell wall proteins are important virulence factors. We previously characterized Dse1 as ...
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candida albicans is a common opportunistic pathogen that causes a variety of diseases in immunocompromised hosts. In a pathogen, cell wall proteins are important virulence factors. We previously characterized Dse1 as a cell wall protein necessary for virulence and resistance to cell surface-disrupting agents, such as calcofluor white, chitin deposition, proper adhesion and biofilm formation. In the absence of decomplexation, our objectives were to investigate differential proteomic expression of a DSE1 mutant strain compared to the wild-type strain. The strains were grown under filamentous and non-filamentous conditions. The extracted cell proteome was subjected to tryptic digest, followed by generation of peptide profiles using MALDI-TOF MS. Generated peptide profiles were analysed and unique peaks for each strain and growth condition mined against a candida database, allowing protein identification. The DSE1 mutant was shown to lack the chitin biosynthesis protein chs5, explaining the previously observed decrease in chitin biosynthesis. The wild-type strain expressed Pra1, involved in pH response and zinc acquisition, Atg15, a lipase involved in virulence, and Sod1, required for oxidative stress tolerance, in addition to proteins involved in protein biosynthesis, explaining the increase in total protein content observed compared to the mutants strain. The mutant, on the other hand, expressed glucoamylase 1, a cell wall glycoprotein involved in carbohydrate metabolism cell wall degradation and biofilm formation. As such, MALDI-TOF MS is a reliable technique in identifying mutant-specific protein expression in c. albicans. copyright (c) 2014 John Wiley & Sons, Ltd.
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