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Different modes of stimuli delivery elicit changes in glutamate driven, experience-dependent interneuron response in c. elegans

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NEUROScIENcE RESEARcH 2023年第0期186卷 33-42页

作者： Mabardi, Llian Sato, Hirofumi Toyoshima, Yu Iino, Yuichi Kunitomo, Hirofumi Univ Tokyo Grad Sch Sci Dept Biol Sci 7-3-1 Hongo Bunkyo-ku Tokyo 1130033 Japan

Memory-related neuronal responses are often elicited by sensory stimuli that recapitulate previous experience. Despite the importance of this sensory input processing, its underlying mechanisms remain poorly understood. caenorhabditis elegans chemotax towards salt concentrations experienced in the presence of food. The amphid sensory neurons ASE-left and ASE-right respond to increases and decreases of ambient salt concentration in opposite manners. AIA, AIB and AIY interneurons are post-synaptic to the ASE pair and are thought to be involved in the processing of salt information transmitted from ASE. However, it remains elusive how the responses of these interneurons are regulated by stimulus patterns. Here we show that AIY interneurons display an experience-dependent response to gradual salt concentration changes but not to abrupt stepwise concentration changes. Animals with AIY intact (but AIA and AIB ablated) chemotax towards low salt concentrations similarly to wild-type animals after cultivation with low salt. ASE neurons transmit salt information about the environment through glutamatergic signaling, directing the activity of the interneurons AIY that promote movement towards favorable conditions.

关键词： c elegans chemotaxis Interneuron response Glutamate signaling

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MOTS-c and aerobic exercise induce cardiac physiological adaptation via NRG1/ErbB4/cEBP? modification in rats

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LIFE ScIENcES 2023年第1期315卷 000-000页

作者： Yuan, Jinghan Xu, Bowen Ma, Jiacheng Pang, Xiaoli Fu, Yu Liang, Min Wang, Manda Pan, Yanrong Duan, Yimei Tang, Mi Zhu, Bingmei Laher, Ismail Li, Shunchang Sichuan Univ West China Hosp Regenerat Med Res Ctr Chengdu 610041 Peoples R China Univ Nottingham Fac Sci & Engn Ningbo 315000 Peoples R China Chengdu Sport Univ Inst Sport Med & Hlth Chengdu 610041 Peoples R China Univ British Columbia Fac Med Dept Pharmacol & Therapeut Vancouver BC V6T 1Z3 Canada

Aims: To determine the effects of the mitochondrial open reading frame of the 12S ribosomal RNA type-c (MOTSc) and aerobic exercise on cardiac structure and function and explore the role of neuregulin-1 (NRG1)- ErbB2 receptor tyrosine kinase 4(ErbB4)- ccAAT-enhancer binding protein beta (c/EBP beta) in cardiac physiological adaptation induced by MOTS-c and aerobic *** methods: We used Hematoxylin-Eosin staining(HE)and Transmission Electron Microscope (TEM) to observe the cardiac myocardial structure, carotid artery catheterization to test cardiac function, and real-time quantitative polymerase chain reaction (qRT-PcR) and Western blotting to describe the changes of NRG1, ErbB4, c/ EBP beta, and Gata in cardiac physiological *** findings: MOTS-c and aerobic training significantly increased heart weight and heart weight index (HWI) (all p < 0.05). Aerobic exercise and MOTS-c treatment thickened myocardial fibers, with a tendency of hypertrophy. Heart rate (HR) (p < 0.001, p = 0.010, p = 0.011), the isovolumic diastolic time constant (Tau) (p < 0.001, p < 0.001, p < 0.001) in exercised (E), MOST-c treated (M) and their combination (ME) decreased significantly, while the dP/dtmax (p < 0.001, p < 0.001, p = 0.039) and dP/dtmin (p < 0.001, p < 0.001, p = 0.001) in groups E, M and ME were significantly higher than those in group c, but EDP (p = 0.903, p = 0.708, p = 0.744) remained unchanged. Moreover, c/EBP beta gene levels were significantly decreased in the differential gene expression between groups c and M transcriptomics sequencing. The levels of ErbB4 mRNA (p < 0.001, p < 0.001, p < 0.001) and Gata4 mRNA (p < 0.001, p < 0.001, p = 0.001) in groups E, M and ME increased significantly, while c/EBP beta mRNA expression decreased significantly (p < 0.001, p = 0.002, p = 0.001), which was consistent with the results of transcriptome sequencing. NRG1 mRNA in group E was significantly higher than that in group c (p = 0.003), but there was no significan

关键词： Aerobic exercise cardiac physiological adaptation Neuregulin 1 ErbB4 c EBP? MOTS-c

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Transcriptome analysis of largemouth bass (Micropterus salmoides) challenged with LPS and polyI:c

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FISH & SHELLFISH IMMUNOLOGY 2023年第1期133卷 108534页

作者： Qi, Zhitao Xu, Yang Liu, Yuhao Zhang, Qihuan Wang, Zisheng Mei, Jie Wang, Dezhong Yancheng Inst Technol Jiangsu Key Lab Biochem & Biotechnol Marine Wetlan Yancheng 224051 Jiangsu Peoples R China Hunan Agr Univ Coll Anim Sci & Technol Changsha Hunan Peoples R China Huazhong Agr Univ Coll Fisheries Wuhan 430070 Hubei Peoples R China Sheyang Kangyu Aquat Prod Technol Co Ltd Yancheng 224300 Jiangsu Peoples R China

Largemouth bass (Micropterus salmoides) is a worldwide commercially important aquatic species. In recent years, pathogenic diseases cause great economic losses and hinder the industry of largemouth bass. To further understand the immune response against pathogens in largemouth bass, splenic transcriptome libraries of largemouth bass were respectively constructed at 12 h post-challenged with phosphate-buffered saline (PBS), lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (polyI:c) by using RNA sequencing technology (RNA-seq). RNA libraries were constructed using 9 RNA splenic samples isolated from three biological replicates of the three groups and sequenced on the DNBSEQ platform. A total number of 86,306 unigenes were obtained. Through pairwise comparisons among the three groups, we identified 11,295 different expression genes (DEGs) exhibiting significant differences at the transcript level. There were 7, 7, and 13 signal pathways were significantly enriched in LPS-PBS comparison, polyI:c-PBS comparison, and LPS-polyI:c comparison, respectively, indicating that the immune response to different pathogens was distinct in largemouth bass. To the best of our knowledge, this is the first report on the immune response of largemouth bass against different pathogenassociated molecular patterns (PAMPs) stimuli using transcriptomic analysis. Our results provide a valuable resource and new insights to understanding the immune characteristics of largemouth bass against different pathogens.

关键词： Micropterus salmoides Transcriptome LPS polyI c Different expression genes

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An updated c. elegans nuclear body muscle transcriptome for studies in muscle formation and function

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SKELETAL MUScLE 2023年第1期13卷 1页

作者： Schorr, Anna L. Mejia, Alejandro Felix Miranda, Martina Y. Mangone, Marco Arizona State Univ Mol & Cellular Biol Grad Program Tempe AZ 85281 USA Arizona State Univ Virginia G Piper Ctr Personalized Diagnost Biodesign Inst 1001 S McAllister Ave Tempe AZ 85281 USA Arizona State Univ Sch Life Sci 751 E Lemon Mall Tempe AZ 85287 USA Translat Genom Res Inst 445 N 5th St 4th Floor Phoenix AZ 85004 USA

The body muscle is an important tissue used in organisms for proper viability and locomotion. Although this tissue is generally well studied and characterized, and many pathways have been elucidated throughout the years, we still lack a comprehensive understanding of its transcriptome and how it controls muscle development and function. Here, we have updated a nuclear FAcS sorting-based methodology to isolate and sequence a high-quality muscle transcriptome from caenorhabditis elegans mixed-stage animals. We have identified 2848 muscle-specific protein-coding genes, including 78 transcription factors and 206 protein-coding genes containing an RNA binding domain. We studied their interaction network, performed a detailed promoter analysis, and identified novel muscle-specific cis-acting elements. We have also identified 16 high-quality muscle-specific miRNAs, studied their function in vivo using fluorochrome-based analyses, and developed a high-quality c. elegans miRNA interactome incorporating other muscle-specific datasets produced by our lab and *** study expands our understanding of how muscle tissue functions in c. elegans andin turn provides results that can in the future be applied to humans to study muscular-related diseases.

关键词： miRNAs 3 ' UTR Body muscle tissue Interactome c elegans

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A pathogenic nonsense mutation (c.1522c>T) of the MYBPc3 gene is implicated with hypertrophic cardiomyopathy

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ESc HEART FAILURE 2023年第4期10卷 2711-2717页

作者： Ni, Erru Wang, Tao Zhang, Xuan Xie, Huabin Xiamen Univ Xiamen Cardiovasc Hosp Sch Med Dept Clin Lab Xiamen Peoples R China Xiamen Key Lab Precis Med Cardiovasc Dis Xiamen Peoples R China Xiamen Univ Sch Med Xiamen Peoples R China Dian Diagnost Grp Co Ltd Key Lab Digital Technol Med Diagnost Zhejiang Prov Hangzhou Peoples R China Xiamen Univ Xiamen Cardiovasc Hosp Sch Med Dept Clin Lab Xiamen Fujian Peoples R China

Hypertrophic cardiomyopathy (HcM), a genetically and clinically heterogeneous cardiomyopathy, is commonly caused by mutations in the MYBPc3 gene or other various sarcomeric genes. HcM patients carrying sarcomeric gene mutations may experience an asymptomatic period at early stage but still possess an escalating risk of developing adverse cardiac events including sudden cardiac death. It is crucial to determine the phenotypic and pathogenic effects of mutations in sarcomeric genes. In this study, a 65-year-old male was admitted with a history of chest pain, dyspnoea, and syncope and with a family history of HcM and sudden cardiac death. On admission, electrocardiogram indicated atrial fibrillation and myocardial infarction. Transthoracic echocardiography revealed left ventricular concentric hypertrophy and systolic dysfunction (48%), which were ascertained by cardiovascular magnetic resonance. With late gadolinium-enhancement imaging, cardiovascular magnetic resonance found myocardial fibrosis on left ventricular wall. The exercise stress echocardiography test showed non-obstructive myocardial changes. Whole-exome sequencing analysis identified a MYBPc3 gene heterozygous nonsense variant (c.1522c>T) in the patient and one of his healthy grandnieces (18-year-old). The patient was diagnosed with non-obstructive HcM, heart failure, atrial fibrillation, and so on. Medications, IcD implantation, and catheter ablation were chosen to maintain heart function. Our study provides the clinical evidence regarding the HcM pathogenicity of MYBPc3 c.1522c>T variant and highlights the significance of family genetic testing in the diagnosis and management of HcM.

关键词： MYBPc3 c 1522c>T Exon 16 Hypertrophic cardiomyopathy Nonsense mutation WES

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Melocoton: A Program Logic for Verified Interoperability Between Ocaml and c

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PROcEEDINGS OF THE AcM ON PROGRAMMING LANGUAGES-PAcMPL 2023年第OOPSLA期7卷 716–744页

作者： Gueneau, Armael Hostert, Johannes Spies, Simon Sammler, Michael Birkedal, Lars Dreyer, Derek Univ Paris Saclay ENS Paris Saclay CNRS InriaLab Methodes Formelles F-91190 Gif Sur Yvette France Saarland Univ Saarbrucken Germany MPI SWS Saarland Informat Campus Saarbrucken Germany Aarhus Univ Aarhus Denmark

In recent years, there has been tremendous progress on developing program logics for verifying the correctness of programs in a rich and diverse array of languages. Thus far, however, such logics have assumed that programs are written entirely in a single programming language. In practice, this assumption rarely holds since programs are often composed of components written in different programming languages, which interact with one another via some kind of foreign function interface (FFI). In this paper, we take the first steps towards the goal of developing program logics for multi-language verification. Specifically, we present Melocoton, a multi-language program verification system for reasoning about Ocaml, c, and their interactions through the Ocaml FFI. Melocoton consists of the first formal semantics of (a large subset of) the Ocaml FFI-previously only described in prose in the Ocaml manual-as well as the first program logic to reason about the interactions of program components written in Ocaml and c. Melocoton is fully mechanized in coq on top of the Iris separation logic framework.

关键词： foreign-function interfaces Ocaml c program logics multi-language semantics garbage collection separation logic angelic non-determinism transfinite step-indexing Iris coq

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Development of textile pattern design by M. c. Escher's tessellation technique using chaekgeori icons

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FASHION AND TEXTILES 2023年第1期10卷 15页

作者： An, Mi Hwa Jang, Ae Ran Jeju Natl Univ Dept Fash Clothing &Textiles 102 Jejudaehakro Jeju Si 63243 Jeju Do South Korea Jeju Natl Univ Dept Fash Clothing & Text 102 Jejudaehakro Jeju Si 63243 Jeju Do South Korea

The purpose of this study is to raise awareness of the uniqueness and excellence of Korea's traditional culture among members of the MZ generation (Millennials and generation Z) and explore the possibility of utilizing archetypes of traditional culture in the contemporary context. To that end, this study develops and proposes unique contemporary textile pattern designs applying M. c. Escher's tessellation technique to the formative characteristics and symbolic meaning of chaekgeori icons. To develop the designs, this study reviews the previous literature and theoretically considers the concept of chaekgeori, its formative characteristics, and Escher's tessellation. After extracting iconsrelated to the themes, stylized iconographic motives were applied to geometric shapes to create repeating units. Then, Escher's tessellation was applied to these repeats to develop twelve contemporarily reimagined textile patterns. To verify the usability of the developed textile pattern designs in products targeting the MZ generation, in-depth interviews were conducted with the MZ generation living in Jeju. The developed textile patterns were mapped onto the selected items to prove the applicability of the developed textile patterns to the items preferred by the MZ generation. The contribution of this study lies in the fact that it develops unique and contemporary textile pattern designs based on the formative characteristics and symbolic meaning of chaekgeori icons and maps the designs of fashion items preferred by the MZ generation, thereby raising awareness of the value and excellence of chaekgeori as a part of Korea's traditional culture and shedding light on the possibility of using traditional cultural archetypes in contemporary contexts.

关键词： chaekgeori Health wishes Icons M c Escher s Tessellation Technique MZ generation Textile pattern design Traditional cultural archetypes Symbolic meaning

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Nosocomial infection of c. auris in cOVID-19 Intensive care Unit in Turkiye and Phylogenetic Analysis of Isolates

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MYcOPATHOLOGIA 2023年第5期188卷 765-773页

作者： Sengel, Buket Erturk Ekren, Berkay Yekta Sayin, Elvan Tigen, Elif Tukenmez Seydaliyeva, Aytan cerikcioglu, Nilgun cinel, Ismail Korten, Volkan Sezerman, Ugur Odabasi, Zekaver Marmara Univ Sch Med Dept Infect Dis & Clin Microbiol Istanbul Turkiye Acibadem Univ Inst Hlth Sci Dept Biostat & Bioinformat Istanbul Turkiye Marmara Univ Sch Med Dept Med Microbiol Istanbul Turkiye Marmara Univ Sch Med Dept Anesthesiol & Reanimat Crit Care Istanbul Turkiye

BackgroundThe difficulties in the identification of c. auris and the delays in the implementation of infection control precautions contribute to outbreaks. This study analyzed 10 patients with cOVID-19 and c. auris candidemia, their characteristic and clinical features and phylogenetic features, and the antifungal susceptibilities of the ***. auris were detected in the cOVID-19 IcU of a university hospital between January and August 2021. Identification to species level was performed using MALDI-TOF MS. Antifungal susceptibilities were determined by the Sensititre YeastOne YO10 panel. The isolates were whole genome sequenced to assess genetic relatedness and a phylogenetic tree was drawn including various c. auris *** mean growth time in blood cultures was 38.8 h. c. auris candidemia developed on the average 27th day of IcU admission. All were susceptible to anidulafungin and micafungin, while they were resistant to fluconazole and amphotericin B. Only three isolates were found to be resistant to caspofungin. All patients died. With the WGS method, all isolates were found in a close resemblance to each other in terms of total nucleotide similarity (with a minimum of 96% pairwise alignment). Our isolates showed the closest similarity to South Asian clade (clade I).conclusionsThis study is the first to evaluate the phylogenetic characteristics of c. auris using WGS and to determine antifungal susceptibilities in Turkiye on cOVID-19 patients. The mortality rate was very high in patients who have both cOVID-19 and c. auris candidemia.

关键词： c auris cOVID-19 Infection control and prevention Whole genome sequencing

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Quantitative description of neuronal calcium dynamics in c. elegans' thermoreception

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BIOSYSTEMS 2023年 223卷 104814页

作者： Mobille, Zachary Follmann, Rosangela Vidal-Gadea, Andres Rosa Jr, Epaminondas Illinois State Univ Dept Phys Normal IL 61790 USA Illinois State Univ Dept Math Normal IL 61790 USA Illinois State Univ Sch Informat Technol Normal IL 61790 USA Illinois State Univ Sch Biol Sci Normal IL 61790 USA

The dynamical mechanisms underlying thermoreception in the nematode c. elegans are studied with a mathematical model for the amphid finger-like ciliated (AFD) neurons. The equations, equipped with Arrhenius temperature factors, account for the worm's thermotaxis when seeking environments at its cultivation temperature, and for the AFD's calcium dynamics when exposed to both linearly ramping and oscillatory temperature stimuli. calculations of the peak time for calcium responses during simulations of pulse-like temperature inputs are consistent with known behavioral time scales of c. elegans.

关键词： Thermoreception Mathematical modeling calcium c elegans

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A novel deletion mutation accompanied by a point mutation in Lamin A/c gene: Screened from a dilated cardiomyopathy family

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PERFUSION-UK 2023年第4期38卷 826-836页

作者： Jia, Hao Sun, Yongxin Yao, Wangchao chen, Zhenhang Yang, Shouguo Wang, chunsheng Lu, Shuyang Fudan Univ Zhongshan Hosp Dept Cardiac Surg 1069 Xietu Rd Shanghai 200032 Peoples R China

Background: There are 30%-40% of patients with dilated cardiomyopathy (DcM) having genetic causes, among which Lamin A and c gene (LMNA) mutation is the second most frequent DcM-related mutation, and Lamin A/c may be involved in the pathogenesis of DcM through the regulation of gene transcription or the direct effect of cell structure. Methods: Echocardiography and electrocardiogram were used to diagnose DcM and arrhythmia in a DcM family. Then, linked mutations on LMNA were screened out by high-throughput sequencing and verified by Sanger sequencing in all research individuals. Meanwhile, Human Genome Variation Society (HGVS) and Integrative Genomics Viewer (IGV) were used to analyse the characteristics of the mutated Lamin A/c protein. Finally, mutated-type and wild-type LMNA plasmid was transfected into Ac-16 cardiomyocytes with the form of a lentivirus vector, and its effect on nucleus and actin was studied by immunofluorescence detection. Results In this study, we found a new frame-shifted mutation of LMNA (***414Alafs*66) linked with another point mutation from a DcM family by using High-throughput sequencing, and this deletion mutation led to a truncation of Lamin A/c. By analysing the clinical characteristics of this DcM family, we found that all DcM patients with arrhythmia were carriers of this co-segregation mutation. In the cytological experiment, we found that the mutated-type transfections showed weaker fluorescent intensities on both actin and cell nucleus. conclusions: A co-segregation mutation of LMNA (Point mutation chr1 156107548 c.1712 G>A and truncated frame-shifted mutation chr1 156106086 c.1240delA) was found from a DcM family, and this type of mutation could participate in the pathogenesis of DcM by affecting the expression of actin.

关键词： dilated cardiomyopathy Lamin A c gene mutation actin cardiopathy family study

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