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Evaluating the Performance of a New Model for Predicting the Growth of clostridium perfringens in cooked, Uncured Meat and Poultry Products under Isothermal, Heating, and Dynamically cooling conditions

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JOURNAL OF FOOD ScIENcE 2016年第7期81卷 M1754-M1765页

作者： Huang, Lihan ARS USDA Eastern Reg Res Ctr 600 E Mermaid Lane Wyndmoor PA 19038 USA

clostridium perfringens type A is a significant public health threat and its spores may germinate, outgrow, and multiply during cooling of cooked meats. This study applies a new c. perfringens growth model in the USDA Integrated Pathogen Modeling Program-Dynamic Prediction (IPMP Dynamic Prediction) Dynamic Prediction to predict the growth from spores of c. perfringens in cooked uncured meat and poultry products using isothermal, dynamic heating, and cooling data reported in the literature. The residual errors of predictions (observation-prediction) are analyzed, and the root-mean-square error (RMSE) calculated. For isothermal and heating profiles, each data point in growth curves is compared. The mean residual errors (MRE) of predictions range from -0.40 to 0.02 Log colony forming units (cFU)/g, with a RMSE of approximately 0.6 Log cFU/g. For cooling, the end point predictions are conservative in nature, with an MRE of -1.16 Log cFU/g for single-rate cooling and -0.66 Log cFU/g for dual-rate cooling. The RMSE is between 0.6 and 0.7 Log cFU/g. compared with other models reported in the literature, this model makes more accurate and fail-safe predictions. For cooling, the percentage for accurate and fail-safe predictions is between 97.6% and 100%. Under criterion 1, the percentage of accurate predictions is 47.5% for single-rate cooling and 66.7% for dual-rate cooling, while the fail-dangerous predictions are between 0% and 2.4%. This study demonstrates that IPMP Dynamic Prediction can be used by food processors and regulatory agencies as a tool to predict the growth of c. perfringens in uncured cooked meats and evaluate the safety of cooked or heat-treated uncured meat and poultry products exposed to cooling deviations or to develop customized cooling schedules. This study also demonstrates the need for more accurate data collection during cooling. Practical Applicationclostridium perfringens is a significant public health hazard, and can be found in many meat and po

关键词： c perfringens cooling evaluation predictive modeling

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Evidence for facultative deciduousness in colophospermum mopane in semi-arid African savannas

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AUSTRAL EcOLOGY 2016年第1期41卷 87-96页

作者： Stevens, Nicola Archibald, Sally A. Nickless, Alecia Swemmer, Anthony Scholes, Robert J. Univ Cape Town Dept Biol Sci ZA-7700 Rondebosch South Africa CSIR Nat Resources & Environm ZA-0001 Pretoria South Africa South African Environm Observat Network Fynbos Node Newlands South Africa Univ Witwatersrand Sch Anim Plant & Environm Sci Johannesburg South Africa South African Environm Observat Network Ndlovu Node Phalaborwa South Africa

Leaf phenology dictates the time available for carbon assimilation, transpiration and nutrient uptake in plants. Understanding the environmental cues that control phenology is therefore vital for predicting climate-related changes to plant and ecosystem function. In contrast to temperate systems, and to a lesser degree, tropical forest systems, the cues initiating leaf drop in tropical savannas are poorly studied. We investigated the cues for leaf fall in a tropical monodominant arid savanna species, colophospermum mopane, using an irrigation experiment. We tracked soil moisture, solar radiation, air temperature, leaf water status, leaf health and leaf carbon balance through the dry season in both irrigated and control plants. Water was the primary cue driving leaf loss of *** rather than temperature or light. Trees watered throughout the dry season retained their canopies. These leaves remained functional and continued photosynthesis throughout the dry season. Leaf carbon acquisition rates did not decline with leaf age but were affected by soil moisture availability and temperature. Leaf loss did not occur when leaf carbon gain was zero, or when a particular leaf carbon threshold was reached. colophospermum mopane is facultatively deciduous as water availability determines leaf drop in this widespread arid savanna species. Obligate deciduosity is not the only successful strategy in climates with a long dry season.

关键词： c mopane irrigation leaf age leaf carbon phenology savanna

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The cREB Transcription Factor controls Transcriptional Activity of the Human RIc8B Gene

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JOURNAL OF cELLULAR BIOcHEMISTRY 2016年第8期117卷 1797-1805页

作者： Maureira, Alejandro Sanchez, Rodolfo Valenzuela, Nicole Torrejon, Marcela Hinrichs, Maria V. Olate, Juan Gutierrez, Jose L. Univ Concepcion Fac Ciencias Biol Dept Bioquim & Biol Mol Concepcion Chile

Proper regulation of gene expression is essential for normal development, cellular growth, and differentiation. Differential expression profiles of mRNA coding for vertebrate Ric-8B during embryo and adult stages have been observed. In addition, Ric-8B is expressed in few cerebral nuclei subareas. These facts point to a dynamic control of RIc8B gene expression. In order to understand the transcriptional regulation of this gene, we searched for cis-elements in the sequence of the human RIc8B promoter region, identifying binding sites for the basic/leucine zipper (bZip) cREB transcription factor family (cRE sites) and c/EBP transcription factor family (c/EBP sites). cRE sites were found clustered near the transcription start site, while the c/EBP sites were found clustered at around 300bp upstream the cRE sites. Here, we demonstrate the ability of cREB1 and c/EBP to bind their respective elements identified in the RIc8B promoter. comparative protein-DNA interaction analyses revealed only the proximal elements as high affinity sites for cREB1 and only the distal elements as high affinity sites for c/EBP. chromatin immunoprecipitation analyses, carried out using a human neuroblastoma cell line, confirmed the preferential association of cREB to the proximal region of the RIc8B promoter. By performing luciferase reporter assays, we found the cRE sites as the most relevant elements for its transcriptional activity. Taken together, these data show the existence of functional cREB and c/EBP binding sites in the human RIc8B gene promoter, a particular distribution of these sites and demonstrate a relevant role of cREB in stimulating transcriptional activity of this gene. J. cell. Biochem. 117: 1797-1805, 2016. (c) 2016 Wiley Periodicals, Inc.

关键词： Ric-8B RIc8B cREB c EBP TRANScRIPTION

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一种低时钟频率下UHF RFID标签芯片PIE解码电路的实现方案

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电子技术应用 2017年第8期43卷 52-54,61页

作者：周芝梅张彤刘亮张海峰北京智芯微电子科技有限公司国家电网公司重点实验室电力芯片设计分析实验室北京100192 北京智芯微电子科技有限公司北京市电力高可靠性集成电路设计工程技术研究中心北京100192

对于标签芯片,降低系统时钟频率是降低功耗、提高通讯距离的最有效手段。首先从理论上按照一种等效判决方法推导出PIE解码电路的更低时钟频率,提出了一种低时钟频率下基于ISO 18000-6 TYPE c协议的UHF RFID标签芯片解码电路的实现方案... 详细信息

对于标签芯片,降低系统时钟频率是降低功耗、提高通讯距离的最有效手段。首先从理论上按照一种等效判决方法推导出PIE解码电路的更低时钟频率,提出了一种低时钟频率下基于ISO 18000-6 TYPE c协议的UHF RFID标签芯片解码电路的实现方案。设计的解码电路大幅度降低了标签芯片解码电路功耗,提高了标签响应灵敏度。

关键词：射频识别标签 PIE 解码电路 ISO18000-6 TYPE c

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Fine-Scale crossover Rate Variation on the caenorhabditis elegans X chromosome

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G3-GENES GENOMES GENETIcS 2016年第6期6卷 1767-1776页

作者： Bernstein, Max R. Rockman, Matthew V. NYU Dept Biol New York NY 10003 USA NYU Ctr Genom & Syst Biol 12 Waverly Pl New York NY 10003 USA

Meiotic recombination creates genotypic diversity within species. Recombination rates vary substantially across taxa, and the distribution of crossovers can differ significantly among populations and between sexes. crossover locations within species have been found to vary by chromosome and by position within chromosomes, where most crossover events occur in small regions known as recombination hotspots. However, several species appear to lack hotspots despite significant crossover heterogeneity. The nematode caenorhabditis elegans was previously found to have the least fine-scale variation in crossover distribution among organisms studied to date. It is unclear whether this pattern extends to the X chromosome given its unique compaction through the pachytene stage of meiotic prophase in hermaphrodites. We generated 798 recombinant nested near-isogenic lines (NILs) with crossovers in a 1.41Mb region on the left arm of the X chromosome to determine if its recombination landscape is similar to that of the autosomes. We find that the fine-scale variation in crossover rate is lower than that of other model species, and is inconsistent with hotspots. The relationship of genomic features to crossover rate is dependent on scale, with Gc content, histone modifications, and nucleosome occupancy being negatively associated with crossovers. We also find that the abundances of 4- to 6-bp DNA motifs significantly explain crossover density. These results are consistent with recombination occurring at unevenly distributed sites of open chromatin.

关键词： crossovers recombination rate variation meiosis c elegans

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Polarity and cell division orientation in the cleavage embryo: from worm to human

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MOLEcULAR HUMAN REPRODUcTION 2016年第10期22卷 691-703页

作者： Ajduk, Anna Zernicka-Goetz, Magdalena Univ Warsaw Dept Embryol Fac Biol Miecznikowa 1 PL-02096 Warsaw Poland Univ Cambridge Dept Physiol Dev & Neurosci Downing St Cambridge CB2 3DY England

cleavage is a period after fertilization, when a 1-cell embryo starts developing into a multicellular organism. Due to a series of mitotic divisions, the large volume of a fertilized egg is divided into numerous smaller, nucleated cells-blastomeres. Embryos of different phyla divide according to different patterns, but molecular mechanism of these early divisions remains surprisingly conserved. In the present paper, we describe how polarity cues, cytoskeleton and cell-to-cell communication interact with each other to regulate orientation of the early embryonic division planes in model animals such as caenorhabditis elegans, Drosophila and mouse. We focus particularly on the Par pathway and the actin-driven cytoplasmic flows that accompany it. We also describe a unique interplay between Par proteins and the Hippo pathway in cleavage mammalian embryos. Moreover, we discuss the potential meaning of polarity, cytoplasmic dynamics and cell-to-cell communication as quality biomarkers of human embryos.

关键词： polarity par proteins Hippo signalling cytoplasmic flow embryo c elegans Drosophila mouse human preimplantation development

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Melittin induces human gastric cancer cell apoptosis via activation of mitochondrial pathway

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World Journal of Gastroenterology 2016年第11期22卷 3186-3195页

作者： Gui-Mei Kong Wen-Hua Tao Ya-Li Diao Peng-Hua Fang Ji-Jun Wang Ping Bo Feng Qian Department of Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesMedical School of Yangzhou University Department of Gastroenterology 1st Hospital of Yangzhou

AIM: To investigate the apoptotic effects of melittin on SGc-7901 cells via activation of the mitochondrial signaling pathway in ***: SGc-7901 cells were stimulated by melittin, and its effect on proliferation and apoptosis of was investigated by methyl thiazolyl tetrazolium assay, morphologic structure with transmission electron microscopy, annexin-V/propidium iodide double-staining assay, measuring mitochondrial membrane potential(MMP) levels, and analyzing reactive oxygen species(ROS) concentrations were analyzed by flow cytometry. cytochrome c(cyt c), apoptosis-inducing factor(AIF), endonuclease G(Endo G), second mitochondria-derived activator of caspases(Smac)/direct IAP binding protein with low isoelectric point(Diablo), and FAS were analyzed by western blot. The expression of caspase-3 and caspase-8 was measured using activity assay ***: Melittin was incubated at 1.0, 2.0, 4.0, or 6.0 μg/m L for 1, 2, 4, 6, or 8 h and showed a timeand concentration-dependent inhibition of SGc-7901 cell growth. Melittin induced SGc-7901 cell apoptosis, which was confirmed by typical morphological changes. Treatment with 4 μg/m L melittin induced early apoptosis of SGc-7901 cells, and the early apoptosis rates were 39.97% ± 3.19%, 59.27% ± 3.94%, and 71.50% ± 2.87% vs 32.63% ± 2.75% for 1, 2, and 4 h vs 0 h(n = 3, P < 0.05); the ROS levels were 616.53% ± 79.78%, 974.81% ± 102.40%, and 1330.94% ± 93.09% vs 603.74% ± 71.99%(n = 3, P < 0.05); the MMP values were 2.07 ± 0.05, 1.78 ± 0.29, and 1.16 ± 0.25 vs 2.55 ± 0.42(n = 3, P < 0.05); caspase-3 activity was significantly higher compared to the control(5492.3 ± 321.1, 6562.0 ± 381.3, and 8695.7 ± 449.1 vs 2330.0 ± 121.9), but the caspase activity of the non-tumor cell line L-O2 was not different from that of the control. With the addition of the caspase-3 inhibitor(Ac-DEVD-cHO), caspase-3 activity was significantly decreased compared to the control group(1067.0 ± 132.5 U/g vs 8695.7 ± 449.1 U/g). The expression

关键词： Melittin Gastric cancer Mitochondrial Apoptosis cytochrome c

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Streamlining Whole Function Vectorization in c using Higher Order Vector Semantics 29

Streamlining Whole Function Vectorization in C using Higher ...

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29th IEEE International Parallel and Distributed Processing Symposium (IPDPS)

作者： Rapaport, Gil Zaks, Ayal Ben-Asher, Yosi Intel Corp Haifa Israel Univ Haifa Dept Comp Sci Haifa Israel

ISBN: (纸本)9781467376846

Taking full advantage of SIMD instructions in c programs still requires tedious and non-portable programming using intrinsics, despite considerable efforts spent developing auto-vectorization capabilities in recent decades. Whole Function Vectorization (WFV) is a recent technique for extending the use of SIMD across entire functions. WFV has so far only been used in data-parallel languages such as OpencL and ISPc. We propose a vector-oriented programming framework that facilitates WFV directly in c. We show that our framework achieves competitive performance to OpencL and ISPc while maintaining c's original syntax and semantics. This allows c programmers to gain better performance for their applications by improving SIMD utilization, without stepping out of c.

关键词： SIMD Whole Function Vectorization c

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Stability and Sensory Quality of Dried Papaya

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Food and Nutrition Sciences 2018年第5期9卷 489-501页

作者： Ronielli cardoso Reis Eliseth de Souza Viana Siomara costa Santana da Silva Maria Eug&ecirc nia de Oliveira Mamede &Iacute dila Maria da Silva Ara&uacute jo National Cassava &amp amp Fruits Research Center Brazilian Agricultural Research Corporation (EMBRAPA) Cruz das Almas Brazil Federal University of Bahia (UFBA) Salvador Brazil State University of Feira de Santana (UEFS) Feira de Santana Brazil Tropical Agroindustry Center Brazilian Agricultural Research Corporation (EMBRAPA) Fortaleza Brazil

Fruit dehydration is a way of supplying the population with healthy and nutritious foods. The shelf life of dried fruit can be defined by the evaluation of changes occurred in chemical characteristics during storage. This study aims to evaluate the sensory quality and the stability of papaya cv. Tainung n° 1 dehydrated by convective drying. Fresh and dried papaya were evaluated for color, moisture, pH, acidity, water activity, soluble solids, vitamin c, carotenoids, total extractable polyphenols (TEP) and antioxidant activity (ABTS). The sensorial acceptance of the dried papaya was evaluated using a structured nine-point hedonic scale. For the stability study, the analysis of moisture, pH, titratable acidity, water activity, total carotenoids and vitamin c were carried out every 30 days of storage until 120 days. During storage, the moisture content of dried papaya remained constant, but there were undesirable changes in color, increase of acidity and reduction of soluble solids. The degradation of total carotenoids and vitamin c followed the first order reaction, and the half-life time was 346 days for carotenoids, whereas for vitamin c it was only 29 days. In the sensory analysis, the dried papaya received grades between 5.0 and 6.0 for all evaluated attributes. Dried papaya is recommended to be consumed up to 30 days, since within this period a product with higher total carotenoids content, vitamin c and with satisfactory physicochemical and sensorial characteristics were obtained.

关键词： carica papaya carotenoids color Dehydrated Fruit Drying Vitamin c

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Anti-aging treatments slow propagation of synucleinopathy by restoring lysosomal function

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AUTOPHAGY 2016年第10期12卷 1849-1863页

作者： Kim, Dong-Kyu Lim, Hee-Sun Kawasaki, Ichiro Shim, Yhong-Hee Vaikath, Nishant N. El-Agnaf, Omar M. A. Lee, He-Jin Lee, Seung-Jae Seoul Natl Univ Coll Med Dept Biomed Sci 103 Daehak Ro Seoul 03080 South Korea Seoul Natl Univ Coll Med Neurosci Res Inst Seoul South Korea Konkuk Univ Dept Biomed Sci & Technol Seoul South Korea Konkuk Univ Dept Biosci & Biotechnoloy Seoul South Korea Konkuk Univ Inst KU Biotechnol Seoul South Korea United Arab Univ Coll Med & Hlth Sci Dept Biochem Al Ain U Arab Emirates QBRI Neurol Disorders Ctr Doha Qatar HBKU Qatar Fdn Coll Sci & Engn Doha Qatar Konkuk Univ Sch Med Dept Anat Seoul South Korea Celltrion Inc Inchon South Korea

Aging is the major risk factor for neurodegenerative diseases that are also associated with impaired proteostasis, resulting in abnormal accumulation of protein aggregates. However, the role of aging in development and progression of disease remains elusive. Here, we used caenorhabditis elegans models to show that aging-promoting genetic variations accelerated the rate of cell-to-cell transmission of SNcA/-synuclein aggregates, hallmarks of Parkinson disease, and the progression of disease phenotypes, such as nerve degeneration, behavioral deficits, and reduced life span. Genetic and pharmacological anti-aging manipulations slowed the spread of aggregates and the associated phenotypes. Lysosomal degradation was significantly impaired in aging models, while anti-aging treatments reduced the impairment. Transgenic expression of hlh-30p::hlh-30, the master controller of lysosomal biogenesis, alleviated intercellular transmission of aggregates in the aging model. Our results demonstrate that the rate of aging closely correlates with the rate of aggregate propagation and that general anti-aging treatments can slow aggregate propagation and associated disease progression by restoring lysosomal function.

关键词： aggregate propagation aging bimolecular fluorescence complementation c elegans lysosome Parkinson disease SNcA -synuclein

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